International Journal of Chemical and Analytical Science最新文献

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Antidermatophytic activity of Eclipta prostrata L. against human infective Trichophyton and Microsporum spp. 黄花对人感染性毛癣菌和小孢子菌的抑菌作用。
International Journal of Chemical and Analytical Science Pub Date : 2013-06-01 DOI: 10.1016/j.ijcas.2013.05.003
Nagabhushan, Koteshwara Anandrao Raveesha, Dyamavvanahalli Lakshmanachar Shrisha
{"title":"Antidermatophytic activity of Eclipta prostrata L. against human infective Trichophyton and Microsporum spp.","authors":"Nagabhushan,&nbsp;Koteshwara Anandrao Raveesha,&nbsp;Dyamavvanahalli Lakshmanachar Shrisha","doi":"10.1016/j.ijcas.2013.05.003","DOIUrl":"https://doi.org/10.1016/j.ijcas.2013.05.003","url":null,"abstract":"<div><p>Emergence of drug resistance is a serious problem in controlling fungal infectious diseases, thus there is a need to look for new drugs from various sources to combat with the problem. Many plants are used in traditional medicine as therapeutic agents which need scientific validation. In the present investigation, <em>Eclipta prostrata</em> L. a known medicinal plant of tropics is evaluated for antifungal potential against species of human infectious <em>Microsporum</em> and <em>Trichophyton</em> following CLSI protocols. Of the five extracts significant activity was observed in petroleum ether extract with a MIC of 0.15 mg mL<sup>−1</sup> against all the test fungi. Attempts to isolate the active principle by TLC revealed 5 bands, but none of the bands showed activity in bioautography, suggesting the synergistic effect. Further work is in progress to identify the combination of molecules responsible for the synergism.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 2","pages":"Pages 136-138"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90029161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
MM/QM study: Interactions of copper(II) and mercury(II) with food dyes in aqueous solutions MM/QM研究:水溶液中铜(II)和汞(II)与食用染料的相互作用
International Journal of Chemical and Analytical Science Pub Date : 2013-06-01 DOI: 10.1016/j.ijcas.2013.04.003
Ismail Daoud, Mourad Mesmoudi, Said Ghalem
{"title":"MM/QM study: Interactions of copper(II) and mercury(II) with food dyes in aqueous solutions","authors":"Ismail Daoud,&nbsp;Mourad Mesmoudi,&nbsp;Said Ghalem","doi":"10.1016/j.ijcas.2013.04.003","DOIUrl":"10.1016/j.ijcas.2013.04.003","url":null,"abstract":"<div><h3>Aim</h3><p>In this work we studied four azo dyes which are proposed for synthetic colorants in foods applications and named <strong>L</strong><sub><strong>1</strong></sub>: Sunset Yellow, E110, <strong>L</strong><sub><strong>2</strong></sub>: Tartrazin, E102, <strong>L</strong><sub><strong>3</strong></sub>: Amaranth, E123, <strong>L</strong><sub><strong>4</strong></sub>: Red Ponceau 4R, E124. In addition, electron density analysis has been carried out to highlight the possible strengths of interaction of these four azo dyes with metal ions.</p></div><div><h3>Materials and methods</h3><p>Density Functional Theory (DFT) was utilized, using the B3LYP functional and the 6-31G(d) basis set. This level of calculation was used to find the optimized molecular structure and to predict the molecular orbitals energies, dipole moment, Fukui function values <span><math><msub><mrow><msup><mi>f</mi><mo>−</mo></msup></mrow><mi>k</mi></msub></math></span>, locals nucleophilicity indexes N<sub>k</sub> and the chemical reactivity parameters that arise from Conceptual DFT. Also, the steric energies values from the four complexes were calculated with the EMO program using MM2 method.</p></div><div><h3>Results</h3><p>Complexes forms with mercury are easier to form and more stable than copper. These show that the mercury intoxication is faster than the copper, knowing that mercury is toxic even with low dose. For that, the men who carry amalgams, we advise may be avoid to eat food and drugs which contain azo dyes because there exists a high trend between these azo dyes and the metal ions which released by the amalgams.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 2","pages":"Pages 49-56"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.04.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73162231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
A rational approach for HPLC calibration using mass spectrometer as detector 一种以质谱仪为检测器的高效液相色谱定标方法
International Journal of Chemical and Analytical Science Pub Date : 2013-06-01 DOI: 10.1016/j.ijcas.2013.04.001
Paresh Varshney , Atul Kaushik , Alok Sharma , Sajal Srivastava , Parmesh Kumar Dwivedi
{"title":"A rational approach for HPLC calibration using mass spectrometer as detector","authors":"Paresh Varshney ,&nbsp;Atul Kaushik ,&nbsp;Alok Sharma ,&nbsp;Sajal Srivastava ,&nbsp;Parmesh Kumar Dwivedi","doi":"10.1016/j.ijcas.2013.04.001","DOIUrl":"10.1016/j.ijcas.2013.04.001","url":null,"abstract":"<div><h3>Background/Objective</h3><p>A direct, accurate, precise, reproducible and cost-time efficient approach for calibration of HPLC using mass spectrometer as detector has been described which simulates the routine analysis in bioanalytical laboratories.</p></div><div><h3>Methods</h3><p>Injector position check and injection volume repeatability test for the calibration of autosampler; and pump flow rate accuracy test for the calibration of pumps were illustrated using direct weighing method. Carryover test, pump repeatability test, linearity and precision were carried out for holistic (overall system) calibration of the instrument using a sensitive high throughput LC–MS/MS pre-validated method.</p></div><div><h3>Results/Conclusion</h3><p>The results of calibration experiments for individual modules suggested that pumps are yielding accurate flow rate, autosampler is aspirating the correct injection volume irrespective to position or volume, and column oven and autosampler chambers are maintaining steady temperatures. Holistic calibration verified the accuracy, precision, repeatability and reproducibility of the instrument with insignificant carryover.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 2","pages":"Pages 102-107"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78696144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Utilization of 2T engine oil by Pseudomonas sp. isolated from automobile workshop contaminated soil 汽车车间污染土壤中分离假单胞菌对2T发动机油的利用
International Journal of Chemical and Analytical Science Pub Date : 2013-06-01 DOI: 10.1016/J.IJCAS.2013.05.002
S. Gagandeep, D. Malik
{"title":"Utilization of 2T engine oil by Pseudomonas sp. isolated from automobile workshop contaminated soil","authors":"S. Gagandeep, D. Malik","doi":"10.1016/J.IJCAS.2013.05.002","DOIUrl":"https://doi.org/10.1016/J.IJCAS.2013.05.002","url":null,"abstract":"","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"1 1","pages":"80-84"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89437083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Invitro free radical scavenging, cytotoxic and acetylcholinesterase inhibitory activities of Leucas martinicensis 马提尼松体外自由基清除、细胞毒及乙酰胆碱酯酶抑制活性研究
International Journal of Chemical and Analytical Science Pub Date : 2013-06-01 DOI: 10.1016/j.ijcas.2013.04.005
Ramani Ramalingam , Anisetti Ravinder Nath , Boddupalli Bindu Madhavi , Malothu Nagulu
{"title":"Invitro free radical scavenging, cytotoxic and acetylcholinesterase inhibitory activities of Leucas martinicensis","authors":"Ramani Ramalingam ,&nbsp;Anisetti Ravinder Nath ,&nbsp;Boddupalli Bindu Madhavi ,&nbsp;Malothu Nagulu","doi":"10.1016/j.ijcas.2013.04.005","DOIUrl":"https://doi.org/10.1016/j.ijcas.2013.04.005","url":null,"abstract":"<div><h3>Aim</h3><p>In recent years oxidative stress was found to play the significant role in causing various diseases, including neurodegenerative disorders like, Alzheimer's. Lack of antioxidant mechanisms is the major cause for increased oxidative stress. Flavonoids and phenolic compounds from herbs are going to play efficient role in scavenging the free radicals responsible for oxidative stress. Present investigation deals with the free radical scavenging activity of <em>Leucas</em> species in relation to neurodegenerative disorder.</p></div><div><h3>Methods</h3><p>Ethanolic extract of selected plant was estimated for phenolic and flavonoids contents and then screened for free radical scavenging activity with various oxygen radicals. The extract was further tested <em>invitro</em> for acetylcholinesterase inhibitory activity along with cytotoxic activity.</p></div><div><h3>Results</h3><p>The extract was found to contain 42.28 ± 8.88 RE/g of flavonoids and 38.624 ± 14.171 GAE/g of phenolic compounds along with significant free radical scavenging activity in comparison with the standard Quercetin. The extract was found to be non cytotoxic with no inhibitory activity on acetylcholinesterase.</p></div><div><h3>Conclusion</h3><p>Inspite of its inability to inhibit acetylcholinesterase, the selected plant extract can be safely used for treating neurodegenerative disorders like Alzheimer's because of its effective free radical scavenging activity.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 2","pages":"Pages 91-95"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.04.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90126489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
A simple and efficient stereoselective total synthesis of (−)-sedridine 一种简单、高效的立体选择性全合成(−)-赛定胺
International Journal of Chemical and Analytical Science Pub Date : 2013-06-01 DOI: 10.1016/j.ijcas.2013.07.004
Saroja Nuguri , Laxminarayana Eppakayala , Naresh Kumar Vuppula , Prasad Kottapalli Rajasekhara
{"title":"A simple and efficient stereoselective total synthesis of (−)-sedridine","authors":"Saroja Nuguri ,&nbsp;Laxminarayana Eppakayala ,&nbsp;Naresh Kumar Vuppula ,&nbsp;Prasad Kottapalli Rajasekhara","doi":"10.1016/j.ijcas.2013.07.004","DOIUrl":"10.1016/j.ijcas.2013.07.004","url":null,"abstract":"<div><p>An asymmetric synthesis of the piperidine alkaloid (−)-sedridine isolated from Sedum acre, has been accomplished starting from <em>S</em>-malic acid as the chiral precursor employing Maruoka Keck allylation, azide nucleophillic substitution and ring closing metathesis as the key steps.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 2","pages":"Pages 131-135"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.07.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77732892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Development and validation of RP-HPLC method for estimation of Tapentadol hydrochloride in bulk and tablet dosage forms 盐酸他他他多原料药和片剂的反相高效液相色谱测定方法的建立与验证
International Journal of Chemical and Analytical Science Pub Date : 2013-06-01 DOI: 10.1016/j.ijcas.2013.07.001
Y. Indira Muzib , J. Ravi Kumar Reddy , K.P.R. Chowdary , E. Swathi
{"title":"Development and validation of RP-HPLC method for estimation of Tapentadol hydrochloride in bulk and tablet dosage forms","authors":"Y. Indira Muzib ,&nbsp;J. Ravi Kumar Reddy ,&nbsp;K.P.R. Chowdary ,&nbsp;E. Swathi","doi":"10.1016/j.ijcas.2013.07.001","DOIUrl":"10.1016/j.ijcas.2013.07.001","url":null,"abstract":"<div><h3>Objective</h3><p>To develop a simple, novel, sensitive, precise and specific RP-HPLC method for the determination of Tapentadol hydrochloride in bulk and its tablet dosage forms.</p></div><div><h3>Methods</h3><p>The chromatographic separation was achieved on C18 Licrosphere column (150 mm × 4.6 mm inner diameter, 5 μm particle size) as a stationary phase using Methanol: 0.1 mM Dipotassium Phosphate buffer (pH 4, adjusted with ortho phosphoric acid) as mobile phase at detection wavelength 280 nm in isocratic mode at a flow rate of 1 ml/min.</p></div><div><h3>Results</h3><p>The calibration curve for Tapentadol hydrochloride was linear from 75 to 450 μg/ml. The correlation coefficient (<em>r</em><sup>2</sup>) value was found to be 0.9994. Precision study showed % CV value less than 2% in all selected concentrations. The % recoveries of Tapentadol hydrochloride are in the range of 99.96–100.01%. The limit of detection and limit of quantification for Tapentadol hydrochloride were found to be 0.25 μg/ml and 0.75 μg/ml respectively.</p></div><div><h3>Conclusion</h3><p>The developed method has good sensitivity, reproducibility and specificity for the determination of Tapentadol hydrochloride in bulk and its tablet dosage forms. This method was simple, fast, accurate, and precise. Hence this method was validated and found to be suitable for determining the purity of Tapentadol hydrochloride in bulk drugs and pharmaceutical formulations. The proposed validated method was successfully used for the quantitative analysis of commercially available dosage form.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 2","pages":"Pages 67-72"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.07.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83645205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Evaluation of photodegradation efficiency on semiconductor immobilized clay photocatalyst by using probit model approximation 用probit模型近似评价半导体固定化粘土光催化剂的光降解效率
International Journal of Chemical and Analytical Science Pub Date : 2013-06-01 DOI: 10.1016/J.IJCAS.2013.07.005
Jaka Nugraha, I. Fatimah
{"title":"Evaluation of photodegradation efficiency on semiconductor immobilized clay photocatalyst by using probit model approximation","authors":"Jaka Nugraha, I. Fatimah","doi":"10.1016/J.IJCAS.2013.07.005","DOIUrl":"https://doi.org/10.1016/J.IJCAS.2013.07.005","url":null,"abstract":"","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"52 1","pages":"125-130"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82689716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
New HPLC method to detect amitriptyline in the blood of rats on combination treatment 联合用药大鼠血液中阿米替林的高效液相色谱检测方法
International Journal of Chemical and Analytical Science Pub Date : 2013-06-01 DOI: 10.1016/j.ijcas.2013.06.001
Rabie Saad Farag , Mahmoud Zaki Darwish , Wael Mohamed Fathy , Hamdi Ahmed Hammad
{"title":"New HPLC method to detect amitriptyline in the blood of rats on combination treatment","authors":"Rabie Saad Farag ,&nbsp;Mahmoud Zaki Darwish ,&nbsp;Wael Mohamed Fathy ,&nbsp;Hamdi Ahmed Hammad","doi":"10.1016/j.ijcas.2013.06.001","DOIUrl":"https://doi.org/10.1016/j.ijcas.2013.06.001","url":null,"abstract":"<div><p>A rapid, sensitive and specific method based on high performance liquid chromatography (HPLC) has been developed for the simultaneous determination of amitriptyline hydrochloride and Sildenafil citrate in the blood of rats. Sample preparation involved liquid–liquid extraction with n-hexane after alkalified with 0.5 M NaOH. Chromatographic separation was performed on XDB C-18 column with particle size of 5 μm (150 × 4.6 mm) with mobile phase acetonitrile and 20 mM KH<sub>2</sub>PO<sub>4</sub> buffer, pH = 3 using flow rate 1.5 ml/min. Calibration curves were linear within the ranges of 0.25–50 and 0.1–50 μg/ml for amitriptyline hydrochloride and Sildenafil citrate, respectively. This method was successfully applied to the pharmacokinetic study in rats after oral administration of amitriptyline hydrochloride and Sildenafil citrate for 24 h.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 2","pages":"Pages 120-124"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91727171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Determination of fortified resistant maltodextrin in juices by High performance liquid chromatography 高效液相色谱法测定果汁中强化抗麦芽糊精的含量
International Journal of Chemical and Analytical Science Pub Date : 2013-03-01 DOI: 10.1016/j.ijcas.2013.03.005
Alok Sharma, Nikhil Kumar Singh, Meena Sharma, Rahul Singh, Chandrakant Katiyar
{"title":"Determination of fortified resistant maltodextrin in juices by High performance liquid chromatography","authors":"Alok Sharma,&nbsp;Nikhil Kumar Singh,&nbsp;Meena Sharma,&nbsp;Rahul Singh,&nbsp;Chandrakant Katiyar","doi":"10.1016/j.ijcas.2013.03.005","DOIUrl":"10.1016/j.ijcas.2013.03.005","url":null,"abstract":"<div><h3>Background/objectives</h3><p>The importance of food fibers has led to the development of a large and potential market for fiber-rich products and ingredients and in recent years. There is a trend to find new sources of dietary fiber that can be used in the food industry. Resistant maltodextrin is a water soluble fiber and short chain polymer of glucose that are resistant to digestion in the human digestive system.</p></div><div><h3>Method</h3><p>As per the AOAC method for determination of total dietary fiber the estimation of low molecular weight resistant maltodextrin involves many tedious steps which make it lengthy &amp; time consuming.</p></div><div><h3>Results</h3><p>Being colorless, tasteless and of low calorific value, resistant maltodextrin qualifies to be a perfect material to used for dietary fiber fortification purposes. Keeping in view the time of analysis, possibility of analytical errors in multiple processing steps &amp; cost of analysis, a simple procedure for determination of resistant maltodextrin has been developed and validate using High performance liquid chromatograph with refractive index detector (HPLC-RID) in fortified fruit juices in 0range citrus Punch.</p></div><div><h3>Conclusion</h3><p>For the confirmation of newly developed analytical method several samples of juices were also analyzed. The method given specifically measures resistant maltodextrin.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 1","pages":"Pages 39-43"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.03.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74092678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
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