iLIVER Pub Date : 2023-03-01 DOI: 10.1016/j.iliver.2023.01.005

Yibulayin Aini , Tiemin Jiang , Hao Wen

引用次数: 0

Investigation of perceived stress and psychological well-being in liver recipients having immunosuppressive therapy 接受免疫抑制治疗的肝脏受者感知压力和心理健康状况的调查

iLIVER Pub Date : 2023-03-01 DOI: 10.1016/j.iliver.2023.01.001

Semra Bulbuloglu , Pınar Harmanci , Fatma Eti Aslan

{"title":"Investigation of perceived stress and psychological well-being in liver recipients having immunosuppressive therapy","authors":"Semra Bulbuloglu , Pınar Harmanci , Fatma Eti Aslan","doi":"10.1016/j.iliver.2023.01.001","DOIUrl":"https://doi.org/10.1016/j.iliver.2023.01.001","url":null,"abstract":"<div><h3>Background and aims</h3><p>In this study, the relationship between perceived stress and psychological well-being was analyzed in liver transplant recipients having immunosuppressive therapy.</p></div><div><h3>Methods</h3><p>This study was performed as descriptive research with 134 patients who had liver transplantation at the organ transplantation institute of a training and research hospital (<em>n</em> = 134). The Perceived Stress Scale and the Psychological Well-Being Scale were used for the data collection. The research data were analyzed by using the Statistical Package for Social Science 25.0.</p></div><div><h3>Results</h3><p>Of all participant liver transplant recipients, 22.4% were aged 18–27 years and 48–57 years and 80.6% were males. Besides, the participant liver transplant recipients used immunosuppressive drugs such as prednisolone (92.53%), tacrolimus (61.9%), and mycophenolate mofetil (52.98%). It was found that there was a statistically significant relationship between psychological well-being and perceived stress.</p></div><div><h3>Conclusion</h3><p>In this study, it was discerned that perceived stress affected psychological well-being negatively. It is of importance to realize the stress experienced by liver transplant recipients and enhance their psychological well-being. In the process following transplant surgery, the liver transplant recipients should be carefully followed and efforts to improve patient outcomes should be made.</p></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"2 1","pages":"Pages 10-15"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49717414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Free fatty acid-induced miR-22 inhibits gluconeogenesis via SIRT-1-mediated PGC-1α expression in nonalcoholic fatty liver disease 在非酒精性脂肪肝中，游离脂肪酸诱导的miR-22通过sirt -1介导的PGC-1α表达抑制糖异生

iLIVER Pub Date : 2023-03-01 DOI: 10.1016/j.iliver.2023.01.002

Ajay K. Yadav , Teja Naveen Sata , Daksh Verma , Amrendra K. Sah , Amit K. Mishra , Mrinalini , Md. Musa Hossain , Kishor Pant , Senthil K. Venugopal

{"title":"Free fatty acid-induced miR-22 inhibits gluconeogenesis via SIRT-1-mediated PGC-1α expression in nonalcoholic fatty liver disease","authors":"Ajay K. Yadav , Teja Naveen Sata , Daksh Verma , Amrendra K. Sah , Amit K. Mishra , Mrinalini , Md. Musa Hossain , Kishor Pant , Senthil K. Venugopal","doi":"10.1016/j.iliver.2023.01.002","DOIUrl":"https://doi.org/10.1016/j.iliver.2023.01.002","url":null,"abstract":"<div><h3>Background and aims</h3><p>Free fatty acids (FFAs) are one of the important regulators of the progression of nonalcoholic fatty liver disease. The FFAs are shown to modulate the metabolic status of the liver by modulating several cellular pathways in hepatocytes. Here, we elucidated the role of miR-22 in modulating FFAs-mediated gluconeogenesis.</p></div><div><h3>Methods</h3><p>Huh7 and WRL68 cells were transfected with nonspecific miRNA, miR-22 premiRs or anti-miR-22 oligos followed by incubation with palmitic acid, oleic acid, and linoleic acid (300 μM each) for 48 and 72 h after transfection. The expression of miR-22 was performed using real-time polymerase chain reaction and Western blots were performed for SIRT-1, PGC-1α, PEPCK, and glucose-6-phosphatase. Three groups of C57BL/6 mice (6 mice per group) were fed with standard diet, choline sufficient <span>l</span>-amino acid defined diet or choline-deficient <span>l</span>-amino acid defined (CDAA) diet for 6, 18, 32, or 54 weeks. Triglycerides content was measured in the serum. Expression of miR-22 and the protein expression of gluconeogenic enzymes were analyzed in the tissue samples.</p></div><div><h3>Results</h3><p>Incubation of miR-22-transfected cells with FFAs inhibited the expression of SIRT-1, PGC-1α, PEPCK, and glucose-6-phosphatase, while miR-22 expression was increased. These changes were reversed when the cells were transfected with anti-miR-22 oligos. CDAA-fed mice showed the significant increase in triglycerides content and miR-22 expression, while there was an inhibition of SIRT-1, PGC-1α, PEPCK, and glucose-6-phosphatase expression in CDAA-fed mice.</p></div><div><h3>Conclusions</h3><p>These data confirm that FFAs inhibited gluconeogenesis via miR-22-mediated inhibition of SIRT-1, which in turn inhibited PGC-1α in hepatic cells.</p></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"2 1","pages":"Pages 1-9"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49717221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatocellular Carcinoma Gene Expression: The New Era, Where It goes? 肝细胞癌基因表达:新时代，走向何方?

iLIVER Pub Date : 2023-03-01 DOI: 10.1016/j.iliver.2022.12.001

Nourhan Badwei

引用次数: 0

Application of biological big data and radiomics in hepatocellular carcinoma 生物大数据和放射组学在肝癌中的应用

iLIVER Pub Date : 2023-03-01 DOI: 10.1016/j.iliver.2023.01.003

Guoxu Fang , Jianhui Fan , Zongren Ding , Yongyi Zeng

引用次数: 0

A high level of secreted phosphoprotein 1 is associated with macrophage infiltration and poor prognosis in hepatocellular carcinoma 高水平分泌的磷酸化蛋白1与肝细胞癌中巨噬细胞浸润和不良预后有关

iLIVER Pub Date : 2023-03-01 DOI: 10.1016/j.iliver.2023.01.004

Jianping Song , Jingxian Sun , Shuhong Jing , Tingxiao Zhang , Jianlei Wang , Yanfeng Liu

{"title":"A high level of secreted phosphoprotein 1 is associated with macrophage infiltration and poor prognosis in hepatocellular carcinoma","authors":"Jianping Song , Jingxian Sun , Shuhong Jing , Tingxiao Zhang , Jianlei Wang , Yanfeng Liu","doi":"10.1016/j.iliver.2023.01.004","DOIUrl":"https://doi.org/10.1016/j.iliver.2023.01.004","url":null,"abstract":"<div><h3>Background and aims</h3><p>Secreted phosphoprotein 1 (<em>SPP1</em>) functions in several physiological processes. The role of SPP1 expression in the prognosis and tumor immunity of hepatocellular carcinoma (HCC) is unknown. The aim of this study was to investigate the expression pattern of SPP1 in HCC and its correlation with prognosis and tumor immunity.</p></div><div><h3>Methods</h3><p>Clinical and gene expression data of The Cancer Genome Atlas-liver hepatocellular carcinoma (LIHC) cohort and 11 other HCC datasets were collected. The Kaplan–Meier method and Cox regression analysis were used to analyze the prognostic value of <em>SPP1</em>. The DESeq2 package in R was used to analyze <em>SPP1</em>-related genes. Gene Ontology analysis and gene set enrichment analysis were used to determine the biological function of <em>SPP1</em> in HCC. The single sample Gene Set Enrichment Analysis (ssGSEA) method was used to analyze the immune infiltrates of HCC. Illumina human methylation 450 data and level 3 HTSeq-FPKM data from The Cancer Genome Atlas-LIHC were used to analyze the effects of DNA methylation level on <em>SPP1</em> expression.</p></div><div><h3>Results</h3><p><em>SPP1</em> was overexpressed in HCC and correlated with T stage, histological grade, adjacent hepatic tissue inflammation, and vascular invasion in HCC. The analysis of survival rates indicated that high <em>SPP1</em> levels were associated with poor overall survival in HCC. Functional analysis showed that <em>SPP1</em> is related to tumor immunity, especially macrophage infiltration. Aberrant demethylation of the promoter region is one of the mechanisms underlying the increase of <em>SPP1</em> in HCC.</p></div><div><h3>Conclusion</h3><p>Our results indicate that <em>SPP1</em> is an independent prognostic factor for HCC and is correlated with the clinical features and macrophage infiltration in HCC.</p></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"2 1","pages":"Pages 26-35"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49717370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute rejection was observed in a combination therapy of CTLA-4 and PD-1 inhibitors before liver transplantation: A case report and literature review 肝移植前CTLA-4和PD-1抑制剂联合治疗出现急性排斥反应:1例报告并文献复习

iLIVER Pub Date : 2023-03-01 DOI: 10.1016/j.iliver.2023.03.001

Pengpeng Zhang, Tianyin Wang, Hong Liu, X. She, Ke Chen, Yu-jun Zhao, Y. Ming

引用次数: 0

Single-cell transcriptome analysis reveals a cancer-associated fibroblast marker gene signature in hepatocellular carcinoma that predicts prognosis 单细胞转录组分析揭示了肝癌中预测预后的癌症相关成纤维细胞标记基因特征

iLIVER Pub Date : 2023-03-01 DOI: 10.1016/j.iliver.2022.12.002

Hao Chi , Dapeng Chen , Yuliang Zhang , Zilin Cui , Yi Bai , Yamin Zhang

{"title":"Single-cell transcriptome analysis reveals a cancer-associated fibroblast marker gene signature in hepatocellular carcinoma that predicts prognosis","authors":"Hao Chi , Dapeng Chen , Yuliang Zhang , Zilin Cui , Yi Bai , Yamin Zhang","doi":"10.1016/j.iliver.2022.12.002","DOIUrl":"https://doi.org/10.1016/j.iliver.2022.12.002","url":null,"abstract":"<div><h3>Background and aims</h3><p>Hepatocellular carcinoma (HCC) is one of the leading causes of cancer death. Multi-pathway combination therapy is used to treat HCC, and immunotherapy is also a routine part of treatment. As a major component of the tumor microenvironment (TME), cancer-associated fibroblasts (CAFs) actively participate in cancer progression through complex functions. However, because CAFs dynamically change during cancer development, most of the current treatment strategies targeting CAFs fail. We created a prognostic CAF marker gene signature (CAFMGS) to investigate the utility of CAFs as a prognostic factor and therapeutic target.</p></div><div><h3>Methods</h3><p>Gene Expression Omnibus (GEO) single-cell RNA sequencing (Sc-RNA-seq) data were analyzed to identify CAF marker genes in HCC. The Cancer Genome Atlas (TCGA) database was used as a training cohort to construct the CAFMGS model and the International Cancer Genome Consortium (ICGC) dataset was used to validate the CAFMGS.</p></div><div><h3>Results</h3><p>Marker genes in the CAFMGS model were (0.0001-<em>SPP1</em>), (0.0084-<em>VCX3A</em>), (0.0015-<em>HMGA1</em>), (0.0082-<em>PLOD2</em>), and (0.0075-<em>CACYBP</em>). The CAFMGS_score was separated into high-risk and low-risk groups based on the median of the patients’ OS. Univariate and multivariate analyses confirmed that CAFMGS_score was an independent prognostic factor in the training group. CAFMGS_score was a more accurate prognostic indicator compared with clinicopathological score and tumor mutational burden score.</p></div><div><h3>Conclusion</h3><p>CAFMGS offers a fresh perspective on stromal cell marker genes in HCC prognosis and expands our knowledge of CAF heterogeneity and functional diversity, perhaps paving the way for CAF-targeted immunotherapy in HCC patients.</p></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"2 1","pages":"Pages 16-25"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49717179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

When liver disease diagnosis encounters deep learning: Analysis, challenges, and prospects 当肝病诊断遇到深度学习:分析、挑战和前景

iLIVER Pub Date : 2023-03-01 DOI: 10.1016/j.iliver.2023.02.002

Yingjie Tian , Minghao Liu , Yu Sun , Saiji Fu

引用次数: 1

Imaging of pregnancy-related liver diseases 妊娠相关肝病的影像学检查

iLIVER Pub Date : 2023-03-01 DOI: 10.1016/j.iliver.2023.02.003

Zongxue Li, Ze Zhang, Jing Yu, Xia Du, Pinggui Lei, Zhibing Ruan, Bo Gao

引用次数: 0

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