iLIVER最新文献

{"title":"MAGI2-AS3/miR-450b-5p/COLEC10 interaction network: A potential therapeutic and prognostic marker in hepatocellular carcinoma","authors":"Lan-Qing Yao ,&nbsp;Yong-Kang Diao ,&nbsp;Jin-Bo Gong ,&nbsp;Li-Hui Gu ,&nbsp;Jia-Hao Xu ,&nbsp;Ming-Da Wang ,&nbsp;Chao Li","doi":"10.1016/j.iliver.2025.100146","DOIUrl":"10.1016/j.iliver.2025.100146","url":null,"abstract":"<div><h3><em>Background and aims</em></h3><div>Hepatocellular carcinoma (HCC) is a prevalent malignancy with poor prognosis. This study uses integrated bioinformatic analyses to explore potential competing endogenous RNA (ceRNA) network chains in HCC.</div></div><div><h3><em>Methods</em></h3><div>HCC expression profile data were obtained from the Gene Expression Omnibus dataset, and differential expression analysis was conducted to identify differentially expressed mRNAs (DEmRNAs), microRNAs (DEmiRNAs), and long non-coding RNAs (DElncRNAs) between HCC and normal liver tissue samples. Univariate Cox regression analysis was performed to identify mRNAs associated with the prognosis of HCC patients. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were used to classify the identified genes functionally. Cytoscape software was used to construct a protein–protein interaction network. Using the intersection method, a ceRNA network was established to align data from two databases (miRTarBase and miRcode). Pearson correlation analysis was conducted to evaluate the relationships between lncRNAs and mRNAs.</div></div><div><h3><em>Results</em></h3><div>A total of 106 prognosis-related DEmRNAs were identified between HCC and normal samples. A total of 132 DEmiRNAs and 42 DElncRNAs were dysregulated in HCC. A ceRNA network of three lncRNAs, six miRNAs, and eight mRNAs was constructed. High expression of MCM10, CDKN3, RRM2, KIF3A, and ALYREF correlated with a poor prognosis, while high expression of CPEB2, COLEC10, and PBLD was associated with a better prognosis for HCC patients. Expression analysis confirmed the differential expression of these genes in HCC samples. Correlation analysis revealed that a MAGI2-AS3/hsa-miR-450b-5p/COLEC10 axis might play a crucial role in the progression of HCC.</div></div><div><h3><em>Conclusion</em></h3><div>The ceRNA network constructed could provide insight into HCC tumorigenesis and might lead to new molecular biomarkers for diagnosing and treating HCC.</div></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"4 1","pages":"Article 100146"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrast-enhanced US and contrast-enhanced CT for diagnosis of focal liver lesions in liver transplant recipients: A comparative study","authors":"Nong Gao ,&nbsp;Dongli Wang ,&nbsp;Xiuzhu Ma ,&nbsp;Faqin Lv ,&nbsp;Xiuyun Ren","doi":"10.1016/j.iliver.2025.100147","DOIUrl":"10.1016/j.iliver.2025.100147","url":null,"abstract":"<div><h3>Background and aims</h3><div>Contrast-enhanced ultrasound (CEUS) is widely used in the diagnosis of complications after liver transplantation. This study compared the diagnostic efficacy of CEUS with that of contrast-enhanced computed tomography (CECT) for focal liver lesions in liver transplant recipients.</div></div><div><h3>Methods</h3><div>We retrospectively reviewed 115 liver transplant recipients who were diagnosed to have focal liver lesions at our hospital between June 2015 and June 2023. All patients were examined by CEUS and CECT and had a definitive pathological diagnosis. Based on the diagnostic outcomes, the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of these imaging modalities in differentiating between benign and malignant lesions were calculated using a four-table method. Differences in diagnostic efficacy between CEUS and CECT with respect to pathological findings were compared using the chi-squared test and Fisher's exact test. The consistency of diagnosis between these modalities was assessed using the linear weighted kappa test.</div></div><div><h3>Results</h3><div>CEUS identified 79 malignant and 36 benign lesions, while CECT detected 81 malignant and 34 benign lesions. The sensitivity, specificity, positive predictive value, negative predictive value, and overall accuracy of CEUS and CECT in diagnosis of focal lesions in transplanted livers were 95.7% vs 97.2%, 97.2% vs 96.1%, 97.9% vs 97.5%, 97.2% vs 98.6%, and 96.4% vs 97.1%, respectively. Both CEUS and CECT demonstrated comparable diagnostic efficacy (κ = 0.899). CEUS showed strong diagnostic consistency with pathological results (κ = 0.912) and was more effective than CECT in diagnosing focal normal liver tissue and fat infiltration (<em>p</em> &lt; 0.05). Both methods were equally effective for diagnosis of focal inflammatory lesions, infarction, and hemangioma.</div></div><div><h3>Conclusion</h3><div>CEUS is as effective as CECT for diagnosis of benign and malignant lesions in transplanted livers but surpasses CECT in differentiating benign lesions, particularly focal normal liver tissue and fat infiltration.</div></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"4 1","pages":"Article 100147"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased attention to women with drug-induced liver injury: Risk factors and early intervention","authors":"Xiaoru Sun ,&nbsp;Xinrong Zhang ,&nbsp;Muyun Liu","doi":"10.1016/j.iliver.2024.100125","DOIUrl":"10.1016/j.iliver.2024.100125","url":null,"abstract":"<div><div>A recent study by Xiong Yu-Ting and colleagues has unveiled the clinical characteristics and risk factors of acute drug-induced liver injury (DILI). Upon reading the article, we found that there are some differences between female and male patients with DILI in terms of clinical and pathological features, especially in the number of patients, the degree of liver inflammation, and the risk of autoimmune liver disease. Therefore, it is necessary to pay attention to female patients with DILI and their outcomes, and to intervene appropriately in advance. This article analyzes the reasons for the differences between female and male patients with DILI and makes recommendations for patient prognosis.</div></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"3 4","pages":"Article 100125"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142651488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gd-EOB-DTPA-enhanced MRI proves advantageous in selecting surgical candidates for patients with early-stage hepatocellular carcinoma: An analysis in terms of oncological outcomes","authors":"Zhiwei Ye ,&nbsp;Jing Zhao ,&nbsp;Dandan Hu ,&nbsp;Zhoutian Yang ,&nbsp;Jinbin Chen ,&nbsp;Li Xu ,&nbsp;Zhongguo Zhou ,&nbsp;Minshan Chen ,&nbsp;Yaojun Zhang","doi":"10.1016/j.iliver.2024.100117","DOIUrl":"10.1016/j.iliver.2024.100117","url":null,"abstract":"<div><h3>Background and aims</h3><div>To determine the value of preoperative Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI) in comparison with extracellular contrast agent MRI and CT in the selection of surgical candidates among patients with hepatocellular carcinoma (HCC), particularly in terms of oncological outcomes after hepatectomy.</div></div><div><h3>Methods</h3><div>This retrospective study included 542 consecutive patients who underwent radical hepatectomy for HCC. One group underwent preoperative Gd-EOB-DTPA-enhanced MRI, one group underwent contrast-enhanced CT, and one group underwent extracellular contrast agent MRI. We compared oncologic outcomes including recurrence free survival and overall survival between the three groups. Subgroup analyses were also performed to provide more specific candidates or beneficiaries for preoperative EOB-MRI.</div></div><div><h3>Results</h3><div>A total of 244 patients had tumor recurrence, with 55 in the EOB-MRI group, 106 in the Routine-MRI group, and 83 in the CT group (<em>p</em> = 0.010). The numbers with early recurrence (&lt;2 years) in each group were 40 (27.03%), 78 (35.78%), and 62 (35.22%), respectively (<em>p</em> = 0.018). The 1, 2, and 3-year recurrence-free survival (RFS) percentages were 82.4%, 73.0%, and 68.2% in the EOB-MRI group, 70.2%, 64.3%, and 56.9% in the Routine-MRI group, and 76.8%, 64.83%, and 58.9% in the CT group (<em>p</em> = 0.010). The 1, 2, and 3-year overall survival percentages were 89.19%, 83.11%, and 80.41% in the EOB-MRI group, 79.82%, 73.86%, and 67.44% in the Routine-MRI group, and 86.55%, 76.14%, and 69.32% in the CT group (<em>p</em> = 0.016). Subgroup analysis showed significant differences in RFS in patients with solitary tumor &lt;3 cm.</div></div><div><h3>Conclusion</h3><div>Preoperative EOB-MRI is superior to contrast-enhanced CT or extracellular contrast agent MRI for selecting surgical candidates at low risk of recurrence following hepatic resection for HCC.</div></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"3 4","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the role of IER3+ macrophages in the prognosis of liver fibrosis by bulk and single-cell transcriptional analyses","authors":"Ling Chu ,&nbsp;Junbo Zhang ,&nbsp;Ruoyu Meng ,&nbsp;Quan Zhuang","doi":"10.1016/j.iliver.2024.100132","DOIUrl":"10.1016/j.iliver.2024.100132","url":null,"abstract":"<div><h3>Background and aims</h3><div>Liver fibrosis (LF) is the common pathological process of almost all liver diseases, but the pathogenesis is extremely complicated and has not been fully clarified. Therefore, we want to explore LF's complex pathogenesis and identify key genetic markers that can predict LF prognosis.</div></div><div><h3>Methods</h3><div>From Gene Expression Omnibus (GEO, <span><span>https://www.ncbi.nlm.nih.gov/geo/</span><svg><path></path></svg></span>), we contained datasets GSE84044 and GSE130970. And involved GSE15654 whose survival data were for the validation of our model’s prognostic value. Then, Bulk RNA sequencing was used to establish a LF-related prognostic model and to determine key genes.The key genes were later analyzed using single-cell RNA sequencing (scRNA-seq) and the role of macrophages in LF was investigated.</div></div><div><h3>Results</h3><div>We established a comprehensive LF-related prognostic model (FMSig, i.e., fibrosis macrophage-related prognostic signature) containing four genes (IER3, AKR1B10, ADCY1, and PGM1). FMSig can distinguish between survival outcomes in dataset GSE15654. IER3 showed a higher hazard ratio than the other three FMSig genes. Moreover, further scRNA-seq analysis showed that IER3 is highly expressed in myeloid cells while the other three genes were rarely expressed in six immune cell types and other cell clusters. After reclustering the myeloid cells into seven clusters, we found that IER3 was highly expressed in myeloid cell cluster 3, shown to be related to anti-inflammation and lipid metabolism functions. Based on pseudotime analysis, we suggest that myeloid cell type 3 is transformed from myeloid cell type 0 in the liver cirrhosis microenvironment. On immunohistochemical staining, LF showed significantly higher IER3 expression than healthy controls.</div></div><div><h3>Conclusion</h3><div>This study established a predictive model, FMSig, to evaluate LF prognosis, and showed that IER3 and macrophages are related to LF.</div></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"3 4","pages":"Article 100132"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WGCNA combined with machine learning to explore potential biomarkers and treatment strategies for acute liver failure, with experimental validation","authors":"Xinyan Wu ,&nbsp;Xiaomei Zheng ,&nbsp;Gang Ye","doi":"10.1016/j.iliver.2024.100133","DOIUrl":"10.1016/j.iliver.2024.100133","url":null,"abstract":"<div><h3>Background and aims</h3><div>To identify biomarkers to predict acute liver failure and investigate the mechanisms and immune-related pathways linked to its onset and progression.</div></div><div><h3><em>Methods</em></h3><div>We analyzed gene expression differences between patients with acute liver failure (ALF) and controls in the GSE14668 dataset. Clinically relevant modules and key ALF-associated genes were identified using weighted gene co-expression network analysis (WGCNA) in conjunction with differential gene expression (DEG) analysis. Enrichment analysis was carried out and protein–protein interaction networks were constructed to understand the functions and pathways. Six potential diagnostic biomarkers were identified using machine learning algorithms. Diagnostic performance was assessed via column charts and area under the curve calculations. Single-sample gene set enrichment analysis evaluated the relationship between known marker gene sets and potential biomarker expression. We also examined diagnostic biomarker mRNA levels in ALF models <em>in vivo</em> and <em>in vitro</em>. We estimated the relative infiltration levels of 22 immune cell subpopulations in ALF samples, and explored the link between diagnostic biomarkers and infiltrating immune cells.</div></div><div><h3><em>Result</em></h3><div>We found 352 DEGs associated with ALF. WGCNA analysis and intersecting DEGs identified 191 significant ALF-related genes. Machine learning identified <em>HORMAD2</em>, <em>WNT10A</em>, <em>ATP6V1E2</em>, <em>CMBL</em>, <em>ARRDC4</em>, and <em>LPIN2</em> as potential diagnostic biomarkers. Cell experiments and quantitative real-time polymerase chain reaction supported the therapeutic potential of eriodictyol for ALF. Immune infiltration analysis suggested that plasma cells, CD4 memory resting and activated T cells, macrophages, and neutrophils might play roles in the progression of ALF.</div></div><div><h3><em>Conclusion</em></h3><div>We identified <em>HORMAD2</em>, <em>WNT10A</em>, <em>ATP6V1E2</em>, <em>CMBL</em>, <em>ARRDC4</em>, and <em>LPIN2</em>, as diagnostic biomarkers for ALF and demonstrated the effectiveness of eriodictyol for treating ALF. Immune cell infiltration may play a significant role in the pathogenesis and progression of ALF.</div></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"3 4","pages":"Article 100133"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142748568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomic predictors for regression of cirrhosis: Clinical implications and future directions","authors":"Binghua Li,&nbsp;Decai Yu","doi":"10.1016/j.iliver.2024.100116","DOIUrl":"10.1016/j.iliver.2024.100116","url":null,"abstract":"","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"3 4","pages":"Article 100116"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on “Clinical outcomes and histologic findings of patients with hepatocellular carcinoma with durable partial response or durable stable disease after receiving atezolizumab plus bevacizumab”","authors":"Binghua Li ,&nbsp;Qiang Wang ,&nbsp;Weiwei Hu ,&nbsp;Huan Li ,&nbsp;Peng Yan ,&nbsp;Yajuan Cao ,&nbsp;Decai Yu","doi":"10.1016/j.iliver.2024.100130","DOIUrl":"10.1016/j.iliver.2024.100130","url":null,"abstract":"","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"3 4","pages":"Article 100130"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142719874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Speaking the same language in predicting Small-for-Size Syndrome in living donor liver transplantation","authors":"Alfred Wei-Chieh Kow","doi":"10.1016/j.iliver.2024.100129","DOIUrl":"10.1016/j.iliver.2024.100129","url":null,"abstract":"","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"3 4","pages":"Article 100129"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142707081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New approaches to the treatment of metabolic dysfunction-associated steatotic liver with natural products","authors":"Pooja Yadav ,&nbsp;Khushi Quadri ,&nbsp;Renu Kadian ,&nbsp;Aafrin Waziri ,&nbsp;Pankaj Agrawal ,&nbsp;Md Sabir Alam","doi":"10.1016/j.iliver.2024.100131","DOIUrl":"10.1016/j.iliver.2024.100131","url":null,"abstract":"<div><div>Metabolic dysfunction-associated steatotic liver disease (MASLD) is a widespread and potentially severe liver condition characterized by abnormal fat accumulation in the liver unrelated to alcohol consumption. According to the World Health Organization, MASLD is the most prevalent liver disease globally, it affects approximately 25% of the world’s population, nearly two billion individuals. This staggering prevalence underscores the urgent need for effective and safe therapeutic approaches to address this escalating global health concern. Natural products have emerged as promising candidates for preventing and treating MASLD in recent years because of their diverse bioactive compounds and minimal side effects. Well-known natural products such as curcumin, resveratrol, green tea polyphenols, and silymarin exhibit notable hepatoprotective effects by influencing lipid metabolism, mitigating oxidative stress, and reducing inflammation. Ongoing research highlights the potential of phytochemicals from traditional medicinal plants, such as <em>Phyllanthus</em> and <em>Salvia</em>, in ameliorating liver steatosis and fibrosis. These natural products demonstrate the capacity to impede fibrogenesis by interfering with hepatic stellate cell activation, which is pivotal in liver fibrosis development. Recent studies underscore the significance of natural products in modulating the gut–liver axis, where they restore balance to the gut microbiota and enhance intestinal barrier function, which slows the progression of MASLD. Moreover, advancements in nanotechnology facilitate the targeted delivery of natural product-derived compounds, which enhances their bioavailability and therapeutic efficacy. Harnessing the potency of natural products offers a promising avenue for developing novel, safer therapies for MASLD and addressing a critical global health concern with far-reaching implications for public well-being.</div></div>","PeriodicalId":100657,"journal":{"name":"iLIVER","volume":"3 4","pages":"Article 100131"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142748567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}