Food Bioengineering最新文献_第9页

Immunomodulatory activity of mannosylglycerate and two unnatural mannosyl-oligosaccharides obtained from microbial fermentation on RAW264.7 macrophages 微生物发酵获得的甘露糖甘油酯和两种天然甘露糖寡糖对RAW264.7巨噬细胞的免疫调节活性

Food Bioengineering Pub Date : 2022-08-29 DOI: 10.1002/fbe2.12026

Juanjuan Liu, Jiangang Yang, Yan Zeng, Chaoyu Tian, Yan Men, Yuanxia Sun

{"title":"Immunomodulatory activity of mannosylglycerate and two unnatural mannosyl-oligosaccharides obtained from microbial fermentation on RAW264.7 macrophages","authors":"Juanjuan Liu, Jiangang Yang, Yan Zeng, Chaoyu Tian, Yan Men, Yuanxia Sun","doi":"10.1002/fbe2.12026","DOIUrl":"10.1002/fbe2.12026","url":null,"abstract":"Mannosyl compounds have been widely used in nutrition, fodder, and vaccine adjuvant industries. In our previous study, the engineered strains for the biosynthesis of three mannosyl compounds including β-1,2-mannobiose (M2-β-1,2), β-1,2-mannotriose (M3-β-1,2), and mannosylglycerate (MG) have been developed. However, their biological activities have not been reported. Here, those three compounds were successfully purified after fermentation of the engineered strains, and their potential immunomodulatory activities on RAW264.7 macrophages were investigated with commercialized β-1,4-mannotriose (M3-β-1,4) as control. Our results showed that M3-β-1,2 and MG promoted the viability and phagocytic function of RAW264.7. Meanwhile, the cytokine TNF-α and interleukin-6 (IL-6) level of RAW264.7 macrophages were significantly enhanced upon the stimulation of M3-β-1,2 and MG compared with M3-β-1,4. Moreover, MG significantly stimulated macrophages to secrete IL-10 compared with other mannan oligosaccharides. Finally, this study proved that the immunomodulatory activity of M3-β-1,2 and MG on RAW 264.7 cells was mainly through mitogen-activated protein kinases and myeloid differentiation protein 88 (MyD88)-dependent signaling pathways. All these findings suggested that M3-β-1,2 and MG exhibited immunomodulatory activities in the innate and adaptive immune systems, thus facilitating the application potential in developing of mannosyl compounds as an immunomodulator available for food and pharmaceutical area.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"1 3-4","pages":"224-232"},"PeriodicalIF":0.0,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76997680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances and prospects in the biosynthesis of vitamin B6 and its bioengineering as a cofactor of other chemicals 维生素B6的生物合成及其作为其他化学物质辅因子的生物工程研究进展与展望

Food Bioengineering Pub Date : 2022-08-03 DOI: 10.1002/fbe2.12022

Lijuan Wu, Linxia Liu, Zhaoxia Jin, Dawei Zhang

{"title":"Advances and prospects in the biosynthesis of vitamin B6 and its bioengineering as a cofactor of other chemicals","authors":"Lijuan Wu, Linxia Liu, Zhaoxia Jin, Dawei Zhang","doi":"10.1002/fbe2.12022","DOIUrl":"10.1002/fbe2.12022","url":null,"abstract":"Vitamin B6 is an essential nutrient, which is widely used in food products, feed additives, pharmaceuticals, disease diagnosis, and other fields. Pyridoxal-5′-phosphate (PLP), the active form of vitamin B6, is an important coenzyme participating in a variety of enzyme reactions. At present, the oxazole method is mainly used for the production of vitamin B6, but toxic and corrosive intermediates produced in the synthesis process, which does not conform to the green manufacturing. Therefore, there is considerable interest in shifting from chemical processes to sustainable fermentation techniques and research on PLP metabolism of other valuable compounds. In this review, we will briefly describe the enzymes that PLP participated and focus on the advances in vitamin B6 biosynthesis and discuss its application to engineering bacteria that overproduce other commercially valuable substances including cadaverine, 3,4-dihydroxyphenylacetate-l-alanine, γ-aminobutyric acid, and l-phosphinothricin. It will provide a reference for the biosynthesis of vitamin B6 or other valuable compounds in which PLP participates in the reaction, and we also look forward to the future development prospect of PLP.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"1 2","pages":"171-181"},"PeriodicalIF":0.0,"publicationDate":"2022-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74139323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Combination of protein engineering and metabolic engineering to enhance (+)-nootkatone production in Saccharomyces cerevisiae 蛋白质工程与代谢工程相结合提高酿酒酵母(+)-诺卡酮产量

Food Bioengineering Pub Date : 2022-08-01 DOI: 10.1002/fbe2.12021

Jingyan Guo, Wei Zhou, Yuanyi Li, Weichuang Qin, Dongzhi Wei, Fengqing Wang, Bei Gao

{"title":"Combination of protein engineering and metabolic engineering to enhance (+)-nootkatone production in Saccharomyces cerevisiae","authors":"Jingyan Guo, Wei Zhou, Yuanyi Li, Weichuang Qin, Dongzhi Wei, Fengqing Wang, Bei Gao","doi":"10.1002/fbe2.12021","DOIUrl":"10.1002/fbe2.12021","url":null,"abstract":"The introduction of heterologous pathways into microorganisms for high-level synthesis of valuable compounds would be extremely meaningful. However, the endogenous heterologous pathway conversion point frequently serves as a bottleneck that limits product synthesis. (+)-Nootkatone is an important functional sesquiterpenoid with a unique grapefruit flavor, which is frequently used as a fragrance. The low catalytic efficiency of the valencene synthase CnVS, the first heterologous enzyme in the biosynthesis of (+)-nootkatone, is the major challenge that restricts the production of (+)-nootkatone in Saccharomyces cerevisiae. In this study, we modified CnVS on the basis of the catalytic environment and mechanism of the enzyme. The residue M560 involving in alkyl transfer during substrate cyclization was found to affect activity. By tuning the residue, mutant M560L exhibited a 60% increase in the unit cell yield of (+)-valencene, which is the precursor of (+)-nootkatone. Furthermore, multistep strategies of metabolic engineering were employed that transferred the metabolic flux to the target product synthesis pathway and displayed the superimposed effect of metabolic and protein engineering on (+)-nootkatone yield. Finally, multicopy integration of tHMG1 and ERG20ERG20-GSG-CnVSM560L boosted the production of (+)-nootkatone to 85.43 mg/L in flask and 804.96 mg/L (5.29 mg/[L h]) in bioreactor. In conclusion, this study presents a paradigm for constructing microbial cell factories using a combination of protein and metabolic engineering in S. cerevisiae.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"1 2","pages":"192-202"},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87214091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Technological characterization and molecular identification of defined protective starter cultures for fermentation of traditional Rayeb made from camel's milk 传统骆驼奶发酵用保护性发酵剂的工艺特性及分子鉴定

Food Bioengineering Pub Date : 2022-07-28 DOI: 10.1002/fbe2.12020

Rania Abozead, Ehab Kheadr, Nabil Safwat, Samir Salam, Nassra Dabour

{"title":"Technological characterization and molecular identification of defined protective starter cultures for fermentation of traditional Rayeb made from camel's milk","authors":"Rania Abozead, Ehab Kheadr, Nabil Safwat, Samir Salam, Nassra Dabour","doi":"10.1002/fbe2.12020","DOIUrl":"10.1002/fbe2.12020","url":null,"abstract":"Camel milk isolates of lactic acid bacteria were characterized to develop defined starter cultures that could be used for the production of standardized Egyptian Rayeb fermented milk. The isolates have shown a wide diversity of Technological characteristics and antimicrobial activity. Interesting isolates were genetically identified at the species level. Two mixed culture starters composed of Lactiplantibacillus plantarum (FFNL146 and FFNL159) and Lactococcus lactis (FFNL1926 and FFNL2005) along with Leuconostoc pseudomesenteroides FFNL2374 were used in the production of Rayeb from camel milk, cow milk, and their mixture and stored for 14 days under refrigeration. Equally mixed camel and cow milk fermented with both starters had the best results of organoleptic attributes and physicochemical properties compared to samples made of camel milk. This is the first study to present two mixed starter cultures for Rayeb manufacturing. The success of these defined starter cultures will potentially enhance the safety and quality of the Rayeb industry in Egypt.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"1 2","pages":"159-170"},"PeriodicalIF":0.0,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82199137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Advances and prospects of transcription-factor-based biosensors in high-throughput screening for cell factories construction 基于转录因子的生物传感器在细胞工厂高通量筛选中的研究进展与展望

Food Bioengineering Pub Date : 2022-07-04 DOI: 10.1002/fbe2.12019

Changfan Li, Chang Wang, Jiang Zhu, Feng Xue, Xiaoman Sun, Yang Gu

引用次数: 3

Design and characterization of a chimeric alginate lyase for immobilization to produce well-defined oligosaccharides 一种嵌合海藻酸裂解酶的设计和表征，用于固定化以生产定义明确的低聚糖

Food Bioengineering Pub Date : 2022-06-08 DOI: 10.1002/fbe2.12014

Hongxiu Zhang, Qianqian Lyu, Xiaohua Liu, Weizhi Liu

{"title":"Design and characterization of a chimeric alginate lyase for immobilization to produce well-defined oligosaccharides","authors":"Hongxiu Zhang, Qianqian Lyu, Xiaohua Liu, Weizhi Liu","doi":"10.1002/fbe2.12014","DOIUrl":"10.1002/fbe2.12014","url":null,"abstract":"Recently, a novel two-domain alginate lyase (AlyAL01) was cloned from Algibacter sp. and successfully overexpressed in Escherichia coli BL21(DE3). Biochemical analysis showed that the N-terminal carbohydrate-binding module (CBM) of AlyAL01 had no effect on the enzyme activity and product distributions. Therefore, the N-terminal CBM was substituted with CBM3 to confer the designed chimeric protein with the ability to specifically bind to regenerated amorphous cellulose. As anticipated, the designed chimeric protein (CBM3-L1) exhibited a similar enzyme activity. Moreover, it was found that the CBM3-L1 combined the purification and immobilization in one step with high immobilized efficiency of 65.8%. The immobilized enzyme exhibited good storage stability and moderate reusability. The immobilized enzyme could keep 85% activity when incubated at 4°C for 60 days and 70% activity when incubated at 25°C for 30 days. Furthermore, the immobilized CBM3-L1 kept about 50% of its initial activity after being reused five times. Finally, immobilized CBM3-L1 successively produced well-defined alginate oligosaccharides (AOS) with DPs of 2–6 by controlling reaction time. In sum, our current study provided a feasible strategy for well-defined AOS production.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"1 2","pages":"182-191"},"PeriodicalIF":0.0,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86108929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-level expression of xyloglucanase B from Rhizomucor miehei and its application in the preparation of partially hydrolyzed apple pomace xyloglucan 木屑根霉木葡聚糖酶B的高水平表达及其在部分水解苹果渣木葡聚糖制备中的应用

Food Bioengineering Pub Date : 2022-05-26 DOI: 10.1002/fbe2.12012

Nan-nan Wang, Yan-xiao Li, Chun-hua Zhu, Qiao-juan Yan, Ran Shi, Zheng-qiang Jiang

引用次数: 1

A rice protein hydrolase from Serratia marcescens and its specificity in preparation of oligopeptide-enriched rice protein hydrolysates 粘质沙雷菌大米蛋白水解酶及其制备富寡肽大米蛋白水解物的特异性

Food Bioengineering Pub Date : 2022-05-21 DOI: 10.1002/fbe2.12013

Zheng-Fei Yan, Jian-Qiao Zhou, Shuai Yuan, Cheng-Ye Tang, Jing Wu

{"title":"A rice protein hydrolase from Serratia marcescens and its specificity in preparation of oligopeptide-enriched rice protein hydrolysates","authors":"Zheng-Fei Yan, Jian-Qiao Zhou, Shuai Yuan, Cheng-Ye Tang, Jing Wu","doi":"10.1002/fbe2.12013","DOIUrl":"10.1002/fbe2.12013","url":null,"abstract":"A secreted 50-kDa metalloprotease from Serratia marcescens D15 was identified as rice protein hydrolase (SeMPase), which reached the highest proteolytic activity (477.3 U/ml) in basal medium with 1.5% rice protein (RP) at 35°C and 150 rpm for 48 h. SeMPase exhibited a highest catalytic number for RP (275.8 min−1, Kcat), which contained a highly conserved Zn2+-binding (HEIGH) domain, Met-turn (SLMSY), and Ca2+ binding domain (GGAGND). RP hydrolysates that prepared by SeMPase showed the highest content of oligopeptides at 72.3%, compared to 64.1% from neutrase, 63.0% from alcalase, 50.2% from trypsin, 47.1% from flavourzyme, and 2.41% from pepsin. Our results showed that cleavage sites of SeMPase exposed in RP are more than those of other proteases because both SeMPase cleavage sites and the amino acid composition of RP are rich in Leu, Val, Phe, Glu, and Ala. SeMPase has significant specificity for the preparation of oligopeptide-enriched RP hydrolysates, which provides a new and highly valued option for industrial preparation of oligopeptide-enriched protein hydrolysates.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"1 2","pages":"126-134"},"PeriodicalIF":0.0,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83660418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perturbing electron carriers of green algae Chlamydomonas reinhardtii for efficient physiological and metabolic regulation 绿藻莱茵衣藻干扰电子载体的生理代谢调控

Food Bioengineering Pub Date : 2022-04-21 DOI: 10.1002/fbe2.12010

Dongqing Zhou, Yeling Luo, Yingqiu Zhang, Chenni Zhao, Hui Wu, Jianhua Fan

{"title":"Perturbing electron carriers of green algae Chlamydomonas reinhardtii for efficient physiological and metabolic regulation","authors":"Dongqing Zhou, Yeling Luo, Yingqiu Zhang, Chenni Zhao, Hui Wu, Jianhua Fan","doi":"10.1002/fbe2.12010","DOIUrl":"10.1002/fbe2.12010","url":null,"abstract":"Microalgae are widely used in the field of food bioengineering. There are relatively few studies on the synthesis of intracellular electron carriers in microalgae and their related metabolic effects. In this study, overexpression of Geranyl diphosphate: 4-hydroxybenzoate geranyltransferase (lepgt), and the addition of 4-hydroxybenzoic acid (4-HB) as quinone ring precursor were adopted to regulate intracellular plastoquinone (PQ) and ubiquinone (UQ) levels in model algae Chlamydomonas reinhardtii. Small changes in PQ and UQ contents could regulate cell growth and metabolism. The expression of lepgt in C. reinhardtii significantly reduced the intracellular contents of PQ and UQ by 49.14% and 98.30%, respectively. The expression of lepgt observably reduced the photosystem activity and respiration rate and increased the contents of neutral lipid and starch by 63.68% and 19.42%, respectively. Exogenous low concentration of 4-HB (1 mM) can effectively improve the contents of intracellular electron carriers, promote respiration, slightly reduce photosystem efficiency, and increase the maximum specific growth rate by 16.93%. It decreased the protein and oil contents by 15.48% and 15.10%, as well as changed the composition of long-chain fatty acids. This paper provided a novel strategy for developing microalgal cell factories for future food production by perturbation on electron carriers of green algae.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"1 2","pages":"148-158"},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85189588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cell-cultivated food production and processing: A review 细胞培养食品生产与加工研究进展

Food Bioengineering Pub Date : 2022-04-21 DOI: 10.1002/fbe2.12009

Tyler J. Barzee, Hamed M. El Mashad, Lin Cao, Allan Chio, Zhongli Pan, Ruihong Zhang

{"title":"Cell-cultivated food production and processing: A review","authors":"Tyler J. Barzee, Hamed M. El Mashad, Lin Cao, Allan Chio, Zhongli Pan, Ruihong Zhang","doi":"10.1002/fbe2.12009","DOIUrl":"10.1002/fbe2.12009","url":null,"abstract":"Cells cultivated in bioreactors offer many possibilities for the production of novel and nutritious food products. Scientific and technological advances in cellular agriculture and processing technologies have allowed for the development of new techniques to utilize in vitro animal cells, plant cells, and microorganisms to mimic the organoleptic and nutritional properties of traditional foods as well as to potentially develop entirely new product classes. This review compiles and discusses the state-of-the-art cellular production and processing systems including 3D printing of customizable cell-cultivated food products. In addition to the technological state-of-the art, this article reviews the nutritional characteristics of cell-cultivated foods, introduces examples of new food products, and compiles economic characteristics and environmental impacts of each production system as assessed through technoeconomic analyses and lifecycle assessments. The factors influencing consumer acceptance of cell-cultivated foods are articulated and the potential implications of these new technologies on traditional agricultural industries and food chains are discussed. Lastly, future research and development trajectories are introduced with suggestions for continued development.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"1 1","pages":"4-25"},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79975655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3