Food Bioengineering最新文献

Current Status and Applications of Genome-Scale Metabolic Models of Oleaginous Microorganisms

Food Bioengineering Pub Date : 2024-12-30 DOI: 10.1002/fbe2.12113

Zijian Hu, Jinyi Qian, Yuzhou Wang, Chao Ye

{"title":"Current Status and Applications of Genome-Scale Metabolic Models of Oleaginous Microorganisms","authors":"Zijian Hu, Jinyi Qian, Yuzhou Wang, Chao Ye","doi":"10.1002/fbe2.12113","DOIUrl":"https://doi.org/10.1002/fbe2.12113","url":null,"abstract":"Oleaginous microorganisms have the unique ability to accumulate lipids that can exceed 20% of their dry cell weight under certain conditions. Despite their potential for efficient lipid production, the metabolic pathways involved are not yet fully understood, largely due to the complexity of intracellular processes and the challenges in phenotypic prediction. This review synthesizes the latest research on the application of Genome-scale Metabolic Network Models (GSMMs) to study oleaginous microorganisms, including bacteria, cyanobacteria, yeast, microalgae, and fungi, and provides a comprehensive analysis of how GSMMs have been utilized to decipher the metabolic mechanisms behind lipid accumulation and to identify key genes involved in lipid synthesis. The review highlights the role of GSMMs in predicting cellular behavior, optimizing metabolic engineering strategies, and discusses the future directions and potential of GSMMs in enhancing lipid production in microorganisms. This comprehensive overview not only summarizes the current state of research but also identifies gaps and opportunities for further investigation in the field.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"3 4","pages":"492-511"},"PeriodicalIF":0.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143253585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Lipids and Emulsifiers on the Release of Strawberry Aroma Compounds in Soy-Based Emulsions

Food Bioengineering Pub Date : 2024-12-26 DOI: 10.1002/fbe2.12112

Jiao Xu, Maomao Zeng, Zhaojun Wang, Qiuming Chen, Fang Qin, Jie Chen, Zhiyong He

{"title":"Effects of Lipids and Emulsifiers on the Release of Strawberry Aroma Compounds in Soy-Based Emulsions","authors":"Jiao Xu, Maomao Zeng, Zhaojun Wang, Qiuming Chen, Fang Qin, Jie Chen, Zhiyong He","doi":"10.1002/fbe2.12112","DOIUrl":"https://doi.org/10.1002/fbe2.12112","url":null,"abstract":"To explore the effects of lipids and emulsifiers on the release of strawberry aroma compounds in soy-based emulsions, the release of seven strawberry aroma compounds (limonene, ethyl hexanoate, (Z)-3-hexenyl acetate, ethyl 2-methylbutanoate, ethyl butanoate, (Z)-3-hexenol, and diacetyl) was examined using static headspace gas chromatographic analysis. Sensory evaluations revealed that the incorporation of soy protein isolate (SPI) emulsions led to a marked imbalance in the strawberry flavor profile. Although no direct correlation was found between emulsion viscosity or particle size and flavor release, lipid type and concentration (soybean oil and palm oil), and emulsifier type (sucrose ester, tween 80, and monoglyceride) significantly impacted flavor retention. Specifically, the hydrophilic compound diacetyl exhibited significantly higher retention in soybean oil emulsions (41%) than in palm oil emulsions (34%) (p < 0.05). The retention of esters and limonene increased with lipid content, whereas diacetyl retention decreased. Sucrose ester and tween 80 demonstrated stronger adsorption of ester compounds than monoglyceride. After sucrose ester addition, retention values of ethyl butanoate, limonene, and diacetyl increased by 1.6, 2.1, and 1.8 times, respectively, compared to samples without it. This study provides theoretical insights into the flavor release behavior in soy-based beverages.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"3 4","pages":"482-491"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143253391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saponins Based on Medicinal and Edible Homologous Plants: Biological Activity, Delivery Systems and Its Application in Healthy Foods

Food Bioengineering Pub Date : 2024-12-26 DOI: 10.1002/fbe2.12111

Siwen Chen, Ying Zhou, Huiliang Li, Lijun You, Sandra Pedisić, Ping Shao

引用次数: 0

High Internal Phase Emulsions of Algae Oil Based on Rapeseed Protein via Salt Extraction Combined With Ultrafiltration

Food Bioengineering Pub Date : 2024-12-23 DOI: 10.1002/fbe2.12109

Jiyang Cai, Yuqi Mei, Yunyi Yang, Suqiong Fang, Wenrong Chen, Tao Yang, Zhili Wan, Xiaoquan Yang

{"title":"High Internal Phase Emulsions of Algae Oil Based on Rapeseed Protein via Salt Extraction Combined With Ultrafiltration","authors":"Jiyang Cai, Yuqi Mei, Yunyi Yang, Suqiong Fang, Wenrong Chen, Tao Yang, Zhili Wan, Xiaoquan Yang","doi":"10.1002/fbe2.12109","DOIUrl":"https://doi.org/10.1002/fbe2.12109","url":null,"abstract":"Rapeseed protein, as a valuable plant protein, holds great potential as a natural emulsifier for construction of food-grade high internal phase emulsions (HIPEs). In this work, rapeseed protein, obtained through salt extraction combined with ultrafiltration, was employed as a sole stabilizer to formulate algae oil-based HIPEs. The effects of protein concentration and pH changes on the physicochemical properties of HIPEs are systematically evaluated. The results show that a protein concentration of 0.5 wt% is sufficient to form stable and self-supporting HIPEs. As the protein concentration increases, the droplet size of HIPEs gradually decreases, leading to a more robust structure and enhanced stability. Compared to neutral conditions (pH 7.0), the HIPEs under acidic pH 3.5 exhibit more densely packed emulsion droplets with smaller size and more uniform distribution, contributing to superior mechanical properties (higher G′ and yield stress) as well as preferable thixotropic and creep recovery behaviors, which thereby improve their physical stability during storage, thermal processing, and freeze-thaw cycles. Furthermore, the rapeseed protein-stabilized HIPEs inhibit the oxidation of algae oil, especially at pH 3.5. The results of oral lubrication indicate that the reduction in the friction coefficient is mainly associated with an increase in protein concentration, with minor effect from pH variation. These findings suggest that rapeseed protein is an effective emulsifier for preparing stable and processable HIPEs, especially under acidic conditions, which have great potential for applications in semi-solid emulsion foods or edible oil structuring.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"3 4","pages":"425-437"},"PeriodicalIF":0.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143253234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Compound Probiotics Microcapsules Improve Milk Yield and Milk Quality of Dairy Cows by Regulating Intestinal Flora”

Food Bioengineering Pub Date : 2024-12-08 DOI: 10.1002/fbe2.12110

引用次数: 0

Multiple metabolic engineering of Saccharomyces cerevisiae for the production of lycopene

Food Bioengineering Pub Date : 2024-11-12 DOI: 10.1002/fbe2.12108

Jiaheng Liu, Minxia Song, Xianhao Xu, Yaokang Wu, Yanfeng Liu, Guocheng Du, Jianghua Li, Long Liu, Xueqin Lv

{"title":"Multiple metabolic engineering of Saccharomyces cerevisiae for the production of lycopene","authors":"Jiaheng Liu, Minxia Song, Xianhao Xu, Yaokang Wu, Yanfeng Liu, Guocheng Du, Jianghua Li, Long Liu, Xueqin Lv","doi":"10.1002/fbe2.12108","DOIUrl":"https://doi.org/10.1002/fbe2.12108","url":null,"abstract":"Lycopene is a high-value-added tetraterpenoid, which is widely used in cosmetics, medicine, food, and dietary supplements. The intracellular mevalonate pathway of Saccharomyces cerevisiae provides natural precursors for terpenoid product synthesis, so it is an excellent host for the heterologous production of lycopene. In this study, a recombinant strain named L10 with efficient lycopene production capability was constructed through multiple strategies, such as regulating the gene copy number of key enzymes, increasing nicotinamide adenine dinucleotide phosphate supply, and reducing squalene accumulation. Then, considering that intracellular lycopene accumulation can cause cytotoxicity to S. cerevisiae, we attempted to identify a transporter that can efficiently transport lycopene from intracellular to extracellular space. Molecular docking simulations predicted that the ATP-binding cassette transporter Snq2p may be a potential transporter of lycopene, and its function in promoting lycopene secretion was further determined by overexpression verification. The lycopene secretion titer of the strain L10Z2 overexpressing Snq2p increased to 16.5 times that of the control at the shake-flask level. After optimizing the galactose regulation system, the intracellular and secreted lycopene production of L11Z2 reached 2113.78 and 26.28 mg/L, respectively, after 150 h fed-batch culture in a 3-L bioreactor. This work provides a new research direction for efficient lycopene synthesis in S. cerevisiae cell factory.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"3 4","pages":"397-406"},"PeriodicalIF":0.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143252503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic role of prebiotics and probiotics in gut microbiome health: Mechanisms and clinical applications

Food Bioengineering Pub Date : 2024-10-21 DOI: 10.1002/fbe2.12107

Tapasya Kumari, Kshirod Kumar Bag, Amit Baran Das, Sankar Chandra Deka

{"title":"Synergistic role of prebiotics and probiotics in gut microbiome health: Mechanisms and clinical applications","authors":"Tapasya Kumari, Kshirod Kumar Bag, Amit Baran Das, Sankar Chandra Deka","doi":"10.1002/fbe2.12107","DOIUrl":"https://doi.org/10.1002/fbe2.12107","url":null,"abstract":"Prebiotic and probiotic usage has exploded, with most formulations promoting gastrointestinal and immunological benefits. Prebiotics modulate the gut microbiota, as a result, short-chain fatty acids are released into the bloodstream. Prebiotics have immunomodulatory properties that reduce inflammation while enhancing immune responses and boosting gut health. The potential of probiotics has shown steady expansion in the digestive system, metabolic balance, and vaginal health. Probiotics offer therapeutic and preventative strategies for a range of human diseases. The in vitro studies suggested the delivery matrix might influence their effects through physicochemical interactions with molecular and cellular structures as well as modifications in cellular expression. Dietary fibers and polyphenols both contribute significantly to human health protection and can ferment in the gut microbiota to create butyrate. This comprehensive review aims to highlight the probiotics and prebiotics, and provide evidence to support their use in preventive and therapeutic medicine. It is anticipated that it will help the clinical and preclinical research to look into the effects of inclusion and processing on their activity in different food delivery formulations. There are potential opportunities needed to enhance immunological and digestive health by comprehending and using the interaction between the gut microbiota and the immune system in our diet.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"3 4","pages":"407-424"},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143253147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in metal oxide bio-nanocomposites for sustainable food packaging: Fabrication, applications, and future prospectives

Food Bioengineering Pub Date : 2024-10-13 DOI: 10.1002/fbe2.12106

Jayaprakash Nandhini, Michael Bellarmin, Senthil Siva Prakash, Devarajan Sowmya Sri, Elumalai Karthikeyan

{"title":"Advancements in metal oxide bio-nanocomposites for sustainable food packaging: Fabrication, applications, and future prospectives","authors":"Jayaprakash Nandhini, Michael Bellarmin, Senthil Siva Prakash, Devarajan Sowmya Sri, Elumalai Karthikeyan","doi":"10.1002/fbe2.12106","DOIUrl":"https://doi.org/10.1002/fbe2.12106","url":null,"abstract":"The research on metal oxide bio-nanocomposites for sustainable food packaging has witnessed significant advancements, offering a promising alternative to traditional food packaging materials. This review briefly describes their fabrication techniques, applications, superiority over conventional packaging, challenges, limitations, and potential trends. These new materials are derived by incorporating metal oxide nanoparticles into the biopolymers and show better properties, such as better antimicrobial properties, which are vital in food packaging. The advantages of using metal oxide bio-nanocomposites over typical food packaging films include enhanced mechanical properties, better moisture and oxygen resistance, bacterial resistance, and light protection. These versatile materials not only serve the purpose of properly preserving the quality and possibly even the wholesomeness of packed food products, but they are also environmentally friendly. Moreover, the review presents current developments and areas of use of metal oxide bio-nanocomposites in food packaging and it also proposes future developments to meet the modern challenge of the food industry in the development of advanced packaging technologies.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"3 4","pages":"438-463"},"PeriodicalIF":0.0,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143252606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Update application of enzyme in food processing, preservation, and detection 酶在食品加工、保存和检测中的最新应用

Food Bioengineering Pub Date : 2024-10-02 DOI: 10.1002/fbe2.12105

Huan Wang, Zhongke Sun, Yanli Qi, Yuansen Hu, Zifu Ni, Chengwei Li

引用次数: 0

Bioprocess strategies for enhanced performance in single-use bioreactors for biomolecule synthesis: A biokinetic approach 提高用于生物分子合成的一次性生物反应器性能的生物工艺战略：生物动力学方法

Food Bioengineering Pub Date : 2024-10-01 DOI: 10.1002/fbe2.12104

Debashis Dutta, Prashant Kumar, Ajay Singh, Shankar Khade

{"title":"Bioprocess strategies for enhanced performance in single-use bioreactors for biomolecule synthesis: A biokinetic approach","authors":"Debashis Dutta, Prashant Kumar, Ajay Singh, Shankar Khade","doi":"10.1002/fbe2.12104","DOIUrl":"https://doi.org/10.1002/fbe2.12104","url":null,"abstract":"Single-use bioreactors (SUB) have made a significant impact on the field of bioprocessing, becoming increasingly popular for biomolecule synthesis due to their many advantages, such as minimizing contamination risks and streamlining processes. Extensive research has been conducted on the hydrodynamic conditions within single-use bioreactors, with a focus on parameters like mixing time, oxygen transfer rate, and stress levels to improve cell cultivation procedures. Several studies have demonstrated that SUB can effectively nurture various cell types, including those that generate monoclonal antibodies, yielding outcomes similar to conventional bioreactor systems, thus highlighting their adaptability and effectiveness in biomolecule processing. SUB equipped with wave mechanisms have shown to display comparable metabolic behaviors and fermentation consistency to conventional bioreactors, confirming their dependability in supporting fungal growth and metabolite generation. Mechanical stirring for agitation leads to high shear forces alongside enhanced monitoring and control, influencing microbial physiology and macro-morphologies. This underscores the importance of operational factors such as rocking speed, rocking angle, and gas flow rate. Overall, the integration of single-use bioreactors in biomolecule synthesis is expected to expand, driven by the need for increased yields and cost-effective manufacturing solutions.","PeriodicalId":100544,"journal":{"name":"Food Bioengineering","volume":"3 3","pages":"337-351"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fbe2.12104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0