Engineering Microbiology最新文献_第6页

Engineering Microbiology Pub Date : 2025-03-01 Epub Date: 2025-01-01 DOI: 10.1016/j.engmic.2024.100188

Zhiwei Deng, Zhenbo Yuan, Zhengshan Luo, Yijian Rao

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Genetically engineered bacteria as inflammatory bowel disease therapeutics 作为炎症性肠病疗法的基因工程细菌

Engineering Microbiology Pub Date : 2024-12-01 Epub Date: 2024-09-01 DOI: 10.1016/j.engmic.2024.100167

Zhen-Ping Zou , Xiao-Peng Zhang , Qian Zhang, Bin-Cheng Yin, Ying Zhou, Bang-Ce Ye

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Immobilization of Thermomyces lanuginosus lipase on metal-organic frameworks and investigation of their catalytic properties and stability 在金属有机框架上固定热霉菌脂肪酶并研究其催化特性和稳定性

Engineering Microbiology Pub Date : 2024-12-01 Epub Date: 2024-10-28 DOI: 10.1016/j.engmic.2024.100176

Zeynab Rangraz , Mostafa M. Amini , Zohreh Habibi

{"title":"Immobilization of Thermomyces lanuginosus lipase on metal-organic frameworks and investigation of their catalytic properties and stability","authors":"Zeynab Rangraz , Mostafa M. Amini , Zohreh Habibi","doi":"10.1016/j.engmic.2024.100176","DOIUrl":"10.1016/j.engmic.2024.100176","url":null,"abstract":"<div><div>Surface adsorption is a convenient and readily available method for immobilizing enzymes on metal-organic frameworks (MOFs). Metal-organic framework-5 (MOF-5), isoreticular metal-organic frameworks-3 (IRMOF-3), and multivariate analysis of MOF-5/IRMOF-3 (MMI) with a half-amino group (-NH<sub>2</sub>) were prepared in this study. <em>Thermomyces lanuginosus</em> lipase (TLL) was chosen as a commercially available enzyme for immobilization on the surfaces of these MOFs. Briefly, 1.5 mg of TLL was added to 10 mg of the MOFs, and after 24 h, 67, 74, and 88% of the TLL was immobilized on MOF-5, IRMOF-3, and MMI, respectively. Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, scanning electron microscopy, energy-dispersive X-ray analysis, and Brunauer–Emmett–Teller analysis were used to characterize the resulting biocomposites. TLL@MOF-5, TLL@IRMOF-3, and TLL@MMI exhibited activities of 55, 75, and 110 U/mg, respectively. Investigation of the activity and stability of the prepared biocatalysts showed that TLL immobilized on MMI was 2.34-fold more active than free TLL. TLL@MMI exhibited high stability and activity even under harsh conditions. After 24 h of incubation in a mixture of 50% (v/v) MeOH, TLL@MMI retained 80% of its activity, whereas TLL@MOF-5 and free TLL lost 50 and 60% of their activities, respectively. TLL@MMI was used to synthesize 2-arylidenehydrazinyl-4-arylthiozole derivatives (91–98%) in a one-pot vessel by adding benzaldehydes, phenacyl bromides, and thiosemicarbazide to water. The efficiency of the 4a derivative with free TLL was 43%, whereas that with TLL@MMI was 98%.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100176"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439 委内瑞拉链霉菌 ATCC 15439 整个 CYPome 和 Fdxome 的功能分析

Engineering Microbiology Pub Date : 2024-12-01 Epub Date: 2024-08-13 DOI: 10.1016/j.engmic.2024.100166

Shuai Li , Zhong Li , Guoqiang Zhang , Vlada B. Urlacher , Li Ma , Shengying Li

{"title":"Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439","authors":"Shuai Li , Zhong Li , Guoqiang Zhang , Vlada B. Urlacher , Li Ma , Shengying Li","doi":"10.1016/j.engmic.2024.100166","DOIUrl":"10.1016/j.engmic.2024.100166","url":null,"abstract":"<div><p>Cytochrome P450 enzymes (CYPs or P450s) and ferredoxins (Fdxs) are ubiquitously distributed in all domains of life. Bacterial P450s are capable of catalyzing various oxidative reactions with two electrons usually donated by Fdxs. Particularly in <em>Streptomyces</em>, there are abundant P450s that have exhibited outstanding biosynthetic capacity of bioactive metabolites and great potential for xenobiotic metabolisms. However, no systematic study has been conducted on physiological functions of the whole cytochrome P450 complement (CYPome) and ferredoxin complement (Fdxome) of any <em>Streptomyces</em> strain to date<em>,</em> leaving a significant knowledge gap in microbial functional genomics. Herein, we functionally analyze the whole CYPome and Fdxome of <em>Streptomyces venezuelae</em> ATCC 15439 by investigating groups of single and sequential P450 deletion mutants, single P450 overexpression mutants, and Fdx gene deletion or repression mutants. Construction of an unprecedented P450-null mutant strain indicates that none of P450 genes are essential for <em>S. venezuelae</em> in maintaining its survival and normal morphology. The non-housekeeping Fdx1 and housekeeping Fdx3 not only jointly support the cellular activity of the prototypic P450 enzyme PikC, but also play significant regulatory functions. These findings significantly advance the understandings of the native functionality of P450s and Fdxs as well as their cellular interactions.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100166"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000286/pdfft?md5=a8270b363730ae79371412a1a3077ca0&pid=1-s2.0-S2667370324000286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142148045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Key amino acid residues govern the substrate selectivity of the transporter Xltr1p from Trichoderma reesei for glucose, mannose, and galactose 关键氨基酸残基决定了毛霉菌转运体 Xltr1p 对葡萄糖、甘露糖和半乳糖的底物选择性

Engineering Microbiology Pub Date : 2024-12-01 Epub Date: 2024-05-22 DOI: 10.1016/j.engmic.2024.100151

Wei Ma , Shiyu Yuan , Zixian Wang , Kangle Niu , Fengyi Li , Lulu Liu , Lijuan Han , Xu Fang

{"title":"Key amino acid residues govern the substrate selectivity of the transporter Xltr1p from Trichoderma reesei for glucose, mannose, and galactose","authors":"Wei Ma , Shiyu Yuan , Zixian Wang , Kangle Niu , Fengyi Li , Lulu Liu , Lijuan Han , Xu Fang","doi":"10.1016/j.engmic.2024.100151","DOIUrl":"10.1016/j.engmic.2024.100151","url":null,"abstract":"<div><p>This research identified four amino acid residues (Leu174, Asn297, Tyr301, and Gln291) that contribute to substrate recognition by the high-affinity glucose transporter Xltr1p from <em>Trichoderma reesei</em>. Potential hotspots affecting substrate specificity were selected through homology modeling, evolutionary conservation analyses, and substrate-docking modeling of Xltr1p. Variants carrying mutations at these hotspots were subsequently obtained via in silico screening. Replacement of Leu174 or Asn297 in Xltr1p with alanine resulted in loss of hexose transport activity, indicating that Leu174 and Asn297 play essential roles in hexose transport. The Y301W variant exhibited accelerated mannose transport, but lost galactose transport capacity, and mutation of Gln291 to alanine greatly accelerated mannose transport. These results suggest that amino acids located in transmembrane α-helix 7 (Asn297, Tyr301, and Gln291) play critical roles in substrate recognition by the hexose transporter Xltr1p. Our results will help expand the potential applications of this transporter and provide insights into the mechanisms underlying its function and specificity.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100151"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000146/pdfft?md5=bd1fae594efb50f04e5007f9cb46944d&pid=1-s2.0-S2667370324000146-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141143027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The way to uncovering and utilizing marine microbial resources 发掘和利用海洋微生物资源之路

Engineering Microbiology Pub Date : 2024-12-01 Epub Date: 2024-09-24 DOI: 10.1016/j.engmic.2024.100175

Zhi-Feng Zhang, Meng Li

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Proactive monitoring of changes in the microbial community structure in wastewater treatment bioreactors using phospholipid fatty acid analysis 利用磷脂脂肪酸分析主动监测废水处理生物反应器中微生物群落结构的变化

Engineering Microbiology Pub Date : 2024-12-01 Epub Date: 2024-11-03 DOI: 10.1016/j.engmic.2024.100177

Lawson Mensah , Elise Cartmell , Mandy Fletton , Mark Scrimshaw , Pablo Campo

{"title":"Proactive monitoring of changes in the microbial community structure in wastewater treatment bioreactors using phospholipid fatty acid analysis","authors":"Lawson Mensah , Elise Cartmell , Mandy Fletton , Mark Scrimshaw , Pablo Campo","doi":"10.1016/j.engmic.2024.100177","DOIUrl":"10.1016/j.engmic.2024.100177","url":null,"abstract":"<div><div>Diverse microbial community structures (MCS) in wastewater treatment plants (WWTPs) are vital for effectively removing nutrients and chemicals from wastewater. However, the regular monitoring of MCS in WWTP bioreactors remains unattractive owing to the skill and cost required for deploying modern microbial molecular techniques in the routine assessment of engineered systems. In contrast, low-resolution methods for assessing broad changes in the MCS, such as phospholipid fatty acid (PLFA) analysis, have been used effectively in soil studies for decades. Despite using PLFA analysis in soil remediation studies to capture the long-term effects of environmental changes on MCS, its application in WWTPs, where the microbial mass is dynamic and operational conditions are more fluid, remains limited. In this study, microbial communities in a controlled pilot plant and 12 full-scale activated sludge plants (ASPs) were surveyed over a two-year period using PLFA analysis. This study revealed that changes in the MCS in wastewater bioreactors could be detected using PLFA analysis. The MCS comprised 59 % Gram-negative and 9 % Gram-positive bacteria, 31 % fungi, and 1 % actinomycetes. The abundances of Gram-negative bacteria and fungi were strongly inversely correlated, with an R<sup>2</sup> = 0.93, while the fatty acids cy17:0 and 16:1ω7c positively correlated (R<sup>2</sup> = 0.869). Variations in temperature, solid retention time, and WWTP configuration significantly influenced the MCS in activated sludge reactors. This study showed that WWTP bioreactors can be routinely monitored using PLFA analysis, and changes in the bioreactor profile that may indicate imminent bioreactor failure can be identified.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100177"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Exploring interspecific interaction variability in microbiota: A review 探索微生物群种间相互作用的变异性：综述

Engineering Microbiology Pub Date : 2024-12-01 Epub Date: 2024-11-09 DOI: 10.1016/j.engmic.2024.100178

Zhong Yu , Zhihao Gan , Ahmed Tawfik , Fangang Meng

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Biofuel production from lignocellulose via thermophile-based consolidated bioprocessing 通过基于嗜热菌的综合生物加工技术利用木质纤维素生产生物燃料

Engineering Microbiology Pub Date : 2024-12-01 Epub Date: 2024-09-10 DOI: 10.1016/j.engmic.2024.100174

Yilin Le , Mengqi Zhang , Pengju Wu , Huilei Wang , Jinfeng Ni

{"title":"Biofuel production from lignocellulose via thermophile-based consolidated bioprocessing","authors":"Yilin Le , Mengqi Zhang , Pengju Wu , Huilei Wang , Jinfeng Ni","doi":"10.1016/j.engmic.2024.100174","DOIUrl":"10.1016/j.engmic.2024.100174","url":null,"abstract":"<div><div>The depletion of fossil fuels and their impact on the environment have led to efforts to develop alternative sustainable fuels. While biofuel derived from lignocellulose is considered a sustainable, renewable, and green energy source, enhancing biofuel production and achieving a cost-effective bioconversion of lignocellulose at existing bio-refineries remains a challenge. Consolidated bioprocessing (CBP) using thermophiles can simplify this operation by integrating multiple processes, such as hydrolytic enzyme production, lignocellulose degradation, biofuel fermentation, and product distillation. This paper reviews recent developments in the conversion of lignocellulose to biofuel using thermophile-based CBP. First, advances in thermostable enzyme and thermophilic lignocellulolytic microorganism discovery and development for lignocellulosic biorefinery use are outlined. Then, several thermophilic CBP candidates and thermophilic microbes engineered to drive CBP of lignocellulose are reviewed. CRISPR/Cas-based genome editing tools developed for thermophiles are also highlighted. The potential applications of the Design-Build-Test-Learn (DBTL) synthetic biology strategy for designing and constructing thermophilic CBP hosts are also discussed in detail. Overall, this review illustrates how to develop highly sophisticated thermophilic CBP hosts for use in lignocellulosic biorefinery applications.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100174"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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CRISPR-Cas9-based genome-editing technologies in engineering bacteria for the production of plant-derived terpenoids 基于 CRISPR-Cas9 的基因组编辑技术在生产植物源萜类化合物的细菌工程中的应用

Engineering Microbiology Pub Date : 2024-09-01 Epub Date: 2024-05-28 DOI: 10.1016/j.engmic.2024.100154

Xin Sun , Haobin Zhang , Yuping Jia , Jingyi Li , Meirong Jia

{"title":"CRISPR-Cas9-based genome-editing technologies in engineering bacteria for the production of plant-derived terpenoids","authors":"Xin Sun , Haobin Zhang , Yuping Jia , Jingyi Li , Meirong Jia","doi":"10.1016/j.engmic.2024.100154","DOIUrl":"https://doi.org/10.1016/j.engmic.2024.100154","url":null,"abstract":"<div><p>Terpenoids are widely used as medicines, flavors, and biofuels. However, the use of these natural products is largely restricted by their low abundance in native plants. Fortunately, heterologous biosynthesis of terpenoids in microorganisms offers an alternative and sustainable approach for efficient production. Various genome-editing technologies have been developed for microbial strain construction. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) is the most commonly used system owing to its outstanding efficiency and convenience in genome editing. In this review, the basic principles of CRISPR-Cas9 systems are briefly introduced and their applications in engineering bacteria for the production of plant-derived terpenoids are summarized. The aim of this review is to provide an overview of the current developments of CRISPR-Cas9-based genome-editing technologies in bacterial engineering, concluding with perspectives on the challenges and opportunities of these technologies.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100154"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000171/pdfft?md5=2cfc44e8e076429caeecf8ed97dbd95a&pid=1-s2.0-S2667370324000171-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141291571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0