Chemotherapy最新文献

{"title":"Efficacy and safety study of different doses of linezolid in combination with bedaquiline in the treatment of drug-resistant pulmonary tuberculosis.","authors":"Xin Wang, Dongpeng Geng, Xiaoling Wu, Zhen Guo, Conglong Ma, Xiaonan Ma, Xiaomin Hao, Panyun Xiao","doi":"10.1159/000551380","DOIUrl":"https://doi.org/10.1159/000551380","url":null,"abstract":"<p><strong>Introduction: </strong>Drug-resistant tuberculosis presents a major global health challenge due to limited therapeutic options and significant toxicity of traditional regimens. Linezolid, a core Group A drug per WHO guidelines, demonstrates high efficacy but its optimal dosing is debated due to dose-dependent adverse effects. This study aimed to evaluate the efficacy and safety of different initial doses linezolid in combined with bedaquiline for treating drug-resistant pulmonary tuberculosis.</p><p><strong>Methods: </strong>This retrospective study analyzed patients with drug-resistant tuberculosis treated with bedaquiline and linezolid in China, June 2019-June 2022. Data originated from medical records. Adverse Drug Reactions (ADRs) were categorized using the Common Terminology Criteria for Adverse Events (v5.0). Three groups were formed based on initial linezolid dose: 1200 mg/d (high-dose, n=99), 600 mg/d (low-dose, n=121), and 0 mg/d (control, n=50). Clinical data, epidemiological characteristics, treatment outcomes, adverse events, and prognoses of these groups were compared and analyzed statistically.</p><p><strong>Results: </strong>Our research scrutinized the effects of different initial doses of linezolid and bedaquiline on drug-resistant tuberculosis. No significant differences were noted between high-dose and low-dose groups in 6 months sputum smear negativity, cavitary closure time or time for lesion resorption and reduction. However, a higher incidence of adverse events was observed in the high-dose group (47.47%) compared to the low-dose group (29.75%), with bone marrow suppression, peripheral neuropathy, and optic neuritis being predominant. Notably, the proportion of patients requiring dose adjustment due to adverse events was significantly higher in the high-dose group (74.47%) than in the low-dose group (50%, P=0.021).</p><p><strong>Conclusions: </strong>Initial daily linezolid dose of 600 mg and 1,200 mg in combination with bedaquiline yield equivalent efficacy for drug-resistant pulmonary tuberculosis. The lower dose demonstrated improved tolerability. For the said therapy, we advocate a primary recommendation of a daily linezolid dose of 600 mg.</p>","PeriodicalId":10047,"journal":{"name":"Chemotherapy","volume":" ","pages":"1-17"},"PeriodicalIF":1.7,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can Model-Informed Precision Dosing of Beta-Lactams in Critical Care Improve Clinical Outcomes?","authors":"Robert Oakley, Marta Ulldemolins, Maria Patricia Hernández Mitre, Manuel Colmenero, Natasha Roberts, Jason Roberts, Jeffrey Lipman, Aaron Heffernan","doi":"10.1159/000550822","DOIUrl":"10.1159/000550822","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Critically ill patients exhibit acute physiological changes leading to marked antibiotic exposure variability and an increased probability of either subtherapeutic or potentially toxic exposures. Beta-lactam antibiotics are often affected by these physiological changes, leading to variable and often subtherapeutic exposures. Therefore, methods to increase the probability of a therapeutic exposure may be beneficial for critically ill patients. Model Informed Precision Dosing (MIPD) is an emerging strategy using advanced computer models to individualise dosing in this vulnerable population with or without therapeutic drug monitoring (TDM). By tailoring therapy to the individual patient scenario, MIPD aims to improve clinical outcomes by enhancing the likelihood of effective and safe therapy.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary: &lt;/strong&gt;MIPD-guided beta-lactam antibiotic dosing may improve the probability of attaining target therapeutic exposures and clinical cure rates compared to standard fixed dosing in critically ill patients. Moreover, MIPD allows clinicians to target higher beta-lactam antibiotic exposures while minimising the risk of toxicity, when the goal of treatment is both improving clinical cure rates and suppressing the probability of antibiotic resistance emergence. No differences in mortality and adverse event reduction compared to standard dosing have been currently identified in the literature. However, the exact potential of MIPD has yet to be determined given the significant limitations in the current evidence base, including heterogeneous study designs, small sample sizes, inconsistent implementation strategies, and variable outcome definitions. Clinical practice and future research should optimise MIPD performance by selecting appropriate and innovative beta-lactam antibiotic exposure models, as well as identify methods to instigate early MIPD dosing and timely TDM dose adjustments. This may further improve clinical and economic outcomes for critically ill patients.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Key messages: &lt;/strong&gt;MIPD is a clinical decision making tool that uses pharmacokinetic modelling to guide beta-lactam dosing, with or without TDM input. A small number of observational and randomised beta-lactam MIPD intervention studies suggest that clinical outcomes for critically ill patients are improved. Technical, casemix, implementation, and study design limitations likely contribute to inconsistent findings in studies comparing MIPD to standard care. Stronger signals in improved outcomes are correlated with appropriate pharmacokinetic model and pharmacokinetic/pharmacodynamic (PK/PD) target selection, accompanied with timely dose adjustments in critically ill patients with significant pharmacokinetic variability and/or complex pharmacodynamic profiles. Future studies should more robustly define key features that will optimise patient outcomes including: optimal target exposure, characteristics of patients most likely to ben","PeriodicalId":10047,"journal":{"name":"Chemotherapy","volume":" ","pages":"1-14"},"PeriodicalIF":1.7,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Concern.","authors":"","doi":"10.1159/000549719","DOIUrl":"https://doi.org/10.1159/000549719","url":null,"abstract":"","PeriodicalId":10047,"journal":{"name":"Chemotherapy","volume":" ","pages":"1"},"PeriodicalIF":1.7,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug-Drug Interaction between Toosendanin and Tamoxifen and Its Potential Mechanism.","authors":"Xiao He, Yirong Zhen, Pingfa Lin, Ting Lin, Ying Chang","doi":"10.1159/000550093","DOIUrl":"10.1159/000550093","url":null,"abstract":"<p><strong>Introduction: </strong>Toosendanin has shown anticancer activity in various malignancies, including gynecological cancers. As tamoxifen is a cornerstone therapy for breast and ovarian cancers, this study investigated how toosendanin alters its pharmacokinetics, with the goal of assessing interaction risks and supporting drug development.</p><p><strong>Methods: </strong>A pharmacokinetic study was performed in female Sprague-Dawley rats. The rats received an oral dose of tamoxifen (10 mg/kg), with or without pretreatment with toosendanin (30 or 60 mg/kg). Plasma concentrations of tamoxifen were determined by LC-MS/MS. The effects of toosendanin on the metabolic stability of tamoxifen and cytochrome P450 enzyme (CYP450) activity were investigated using liver microsomes.</p><p><strong>Results: </strong>Toosendanin significantly altered the pharmacokinetic profile of tamoxifen. Concomitant administration of toosendanin (30 and 60 mg/kg) resulted in a dose-dependent increase in the maximum plasma concentration of tamoxifen from 120.67 ± 7.50 to 186.67 ± 6.44 and 235.00 ± 15.96 µg/L, respectively. The half-life was prolonged from 12.55 ± 0.57 to 14.46 ± 0.96 and 16.55 ± 1.02 h, while the clearance rate decreased from 4.92 ± 0.24 to 3.04 ± 0.14 and 2.10 ± 0.07 L/h/kg, respectively. In vitro studies further revealed that toosendanin inhibited the metabolic stability of tamoxifen, as evidenced by a reduction in its intrinsic clearance from 45.36 ± 1.92 to 34.48 ± 1.92 and 28.44 ± 1.21 µL/min/mg protein. Furthermore, toosendanin exhibited a half-maximal inhibitory concentration of 11.50 µ<sc>m</sc> for CYP2D6 activity.</p><p><strong>Conclusion: </strong>This study demonstrates that toosendanin alters the pharmacokinetics of tamoxifen, likely by inhibiting its metabolic stability and CYP2D6 activity. This interaction leads to a potentially significant pharmacokinetic risk when toosendanin is co-administered with tamoxifen, potentially potentiating its exposure.</p>","PeriodicalId":10047,"journal":{"name":"Chemotherapy","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145818452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gaza's First Polio Case in 25 Years: Is Health Infrastructure Collapse Threatening Resilience?","authors":"Francesco Branda, Giancarlo Ceccarelli, Marta Giovanetti, Massimo Ciccozzi, Fabio Scarpa","doi":"10.1159/000541933","DOIUrl":"10.1159/000541933","url":null,"abstract":"","PeriodicalId":10047,"journal":{"name":"Chemotherapy","volume":" ","pages":"9-11"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importance of Sex-Dependent Differences for Dosing Selection and Optimization of Chemotherapeutic Drugs.","authors":"Claire Seydoux, Myriam Briki, Anna D Wagner, Eva Choong, Monia Guidi, Sandro Carrara, Yann Thoma, Françoise Livio, François R Girardin, Catia Marzolini, Thierry Buclin, Laurent A Decosterd","doi":"10.1159/000542461","DOIUrl":"10.1159/000542461","url":null,"abstract":"<p><strong>Background: </strong>Despite major advances in cancer treatment in the past years, there is a need to optimize chemotherapeutic drug dosing strategies to reduce toxicities, suboptimal responses, and the risk of relapse. Most cancer drugs have a narrow therapeutic index with substantial pharmacokinetics variability. Yet, current dosing approaches do not fully account for the complex pathophysiological characteristics of the patients. In this regard, the effect of sex on anticancer chemotherapeutic drugs' disposition is still underexplored. In this article, we review sex differences in chemotherapeutic drug pharmacokinetics; we suggest a novel approach that integrates sex into the traditional a priori body surface area (BSA) dosing selection model, and finally, we provide an overview of the potential benefits of a broader use of therapeutic drug monitoring (TDM) in oncology.</p><p><strong>Summary: </strong>To date, anticancer chemotherapeutic drug dosing is most often determined by BSA, a method widely used for its ease of practice, despite criticism for not accounting for individual factors, notably sex. Anatomical, physiological, and biological differences between males and females can affect pharmacokinetics, including drug metabolism and clearance. At equivalent doses, females tend to display higher circulating exposure and more organ toxicities, which has been formally demonstrated at present for about 20% of chemotherapeutic drugs. An alternative could be the sex-adjusted BSA (SABSA), incorporating a 10% increase in dosing for males and a 10% decrease for females, though this approach still lacks formal clinical validation. Another strategy to reduce treatment-related toxicity and potentially enhance clinical outcomes could be a more widespread use of TDM, for which a benefit has been demonstrated for 5-fluorouracil, busulfan, methotrexate, or thiopurines.</p><p><strong>Key messages: </strong>The inclusion of sex besides BSA in an easy-to-implement formula such as SABSA could improve a priori chemotherapy dosing selection, even though it still requires clinical validation. The a posteriori use of TDM could further enhance treatment efficacy and safety in oncology.</p>","PeriodicalId":10047,"journal":{"name":"Chemotherapy","volume":" ","pages":"92-101"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Drug Monitoring: A Driver to Precision Medicine for Patients with Hematological Malignancies.","authors":"Jannik Stemler, Roger M Brüggemann, Nick A de Jonge","doi":"10.1159/000547073","DOIUrl":"10.1159/000547073","url":null,"abstract":"<p><strong>Background: </strong>Targeted therapies have revolutionized the treatment of hematological malignancies, offering improved efficacy with fewer off-target effects compared to traditional chemotherapy. However, significant pharmacokinetic (PK) and pharmacodynamic (PD) variability exists among patients receiving these therapies.</p><p><strong>Summary: </strong>Therapeutic drug monitoring (TDM) measures drug exposure and thereby helps to adjust the dose of a drug to maintain its concentration within a target range. It is frequently applied for drugs with characteristics like PK variability or narrow therapeutic window, among others, to ensure optimal therapeutic outcome while minimizing adverse effects. Many molecular targeted agents (MTAs) for malignancies, especially tyrosine kinase inhibitors, exhibit significant variability in exposure, and yet are still dosed with a \"one-size-fits-all\" approach. While this is partially culprit to regulatory approval requirements of MTA, it contradicts principles of targeted therapy. PK/PD variability necessitates a personalized approach to dosing in order to optimize therapeutic outcomes and minimize toxicity. TDM provides an avenue to refine dosing strategies based on individual patient characteristics.</p><p><strong>Key messages: </strong>Through incorporation of TDM, treatment of hematological malignancies could move toward target concentration-driven dosing in clinical trials and regulatory frameworks. Establishing target concentrations for MTA requires solid exposure-response and exposure-toxicity analyses in the population of interest. To establish such reference ranges, large populational analyses are necessitated, underlining the importance of the incorporation of such endpoints into phase III trials. Economic restrictions, sample transportation logistics, turnaround times, and interpretation may hinder the application of a TDM-guided dosing approach in routine care. Ultimately, personalized TDM-guided dosing could improve patient outcomes and quality of life through minimizing toxicity.</p>","PeriodicalId":10047,"journal":{"name":"Chemotherapy","volume":" ","pages":"193-199"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoplasmic Reticulum Stress Modulates Therapeutic Responses in Hepatocellular Carcinoma.","authors":"Yi-Li Chen, Chen-Wei Chou, I-Hsiu Liu, Yuh-Harn Wu, Cheng-Yi Chen","doi":"10.1159/000545341","DOIUrl":"10.1159/000545341","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) is one of the primary types of liver cancer, and the mortality trend of HCC patients is estimated to continue rising in the future. Chemotherapy drugs or targeted therapies are considered primary treatment modalities for intermediate-stage or advanced-stage HCC. Although these drugs can extend the survival rate of HCC patients, prolonged treatment often raises concerns about drug resistance or cancer recurrence, leading to undesirable therapeutic outcomes. Drug treatments generally involve promoting cytotoxicity and inhibiting oncogenic signaling pathways, and the response of cancer cells to drug-induced stress situations may potentially impact the effectiveness of treatment. The unfolded protein response (UPR) acts as a cellular stress response mechanism, activating pathways such as DNA repair and autophagy to help cellular survival when cells are damaged. It has also been shown that under sustained or excessive stress, UPR can control cell fate toward programmed cell death, such as apoptosis. Previous studies have found that activation of UPR plays an essential role in cancer cell growth and drug resistance. Various molecules or signaling pathways regulated by the UPR assist cancer cells in responding to anticancer drugs, enabling their survival during treatment.</p><p><strong>Summary: </strong>The present review illustrated genetic molecules or signaling pathways controlled by the UPR and investigates their influence on liver cancer drugs. Moreover, the review also summarizes the partial effects of UPR, including lipid droplet formation and inflammatory stimulation, and their roles in HCC development and drug resistance, respectively.</p><p><strong>Key message: </strong>Unraveling and targeting ER stress provide potential therapeutic strategies for HCC treatment.</p>","PeriodicalId":10047,"journal":{"name":"Chemotherapy","volume":" ","pages":"177-192"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phase I Clinical Study of a Multi-Kinase Inhibitor TG02 Capsule for the Treatment of Recurrent High-Grade Gliomas with Failed Temozolomide Treatment in Chinese Patients.","authors":"Cheng-Cheng Guo, Qun-Ying Yang, Shao-Yan Xi, Jian Zhou, Zhi-Huan Zhou, Xi Cao, Yi-Xiang Liao, Benjamin Xiao-Yi Li, Xiang-Rong Dai, Michael Wong, Yu-Jie Li, Xiao-Hui Yu, Zhong-Ping Chen","doi":"10.1159/000542365","DOIUrl":"10.1159/000542365","url":null,"abstract":"<p><strong>Introduction: </strong>We report the safety, tolerability, pharmacokinetic characteristics and preliminary efficacy of a multi-kinase inhibitor (TG02 capsule) as a new therapy for patients with recurrent high-grade gliomas in China.</p><p><strong>Methods: </strong>This is a single-center, dose-escalation, open-label phase I study, which enrolled patients with recurrent high-grade gliomas who failed to temozolomide. Patients were assigned sequentially into different dose groups and received TG02 every 4 weeks. The dose was increased in a traditional 3 + 3 design. Primary endpoints were the dose-limited toxicity (DLT) and the maximum tolerated dose (MTD).</p><p><strong>Results: </strong>Twelve patients (8 glioblastomas, 4 diffuse astrocytoma) were enrolled between May 2019 and November 2021. Three patients received 100 mg and 9 received 150 mg TG02 twice a week. The plasma concentration of TG02 reached the maximum at 2 h after administration, and the elimination half-life was about 7 h. No DLT occurred and MTD was not defined in this study. Eleven patients had one or more investigator-assessed treatment-related adverse events (TRAEs). The most frequent TRAEs were vomiting (91.7%) and diarrhea (75.0%), and 50% of the patients had grade 3 or 4 adverse events. There were no treatment-related deaths. The median progression-free survival and overall survival were 1.77 (95% confidence interval [CI]: 0.82-4.24) and 9.63 (95% CI: 2.66-not estimated) months, respectively.</p><p><strong>Conclusions: </strong>TG02 capsule 150 mg twice a week is safe and tolerable in Chinese patients with recurrent high-grade gliomas. Patients who failed to temozolomide showed obvious tumor reduction when switching to TG02 capsule. The efficacy of recurrent gliomas warrants further investigation.</p>","PeriodicalId":10047,"journal":{"name":"Chemotherapy","volume":" ","pages":"74-84"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulation of Rad51 Expression and Activity Potentiates the Cytotoxic Effect of Osimertinib in Human Non-Small Cell Lung Cancer Cells.","authors":"Jen-Chung Ko, Jyh-Cheng Chen, Ching-Hsiu Huang, Pei-Jung Chen, Qiao-Zhen Chang, Bo-Cheng Mu, Jun-Jie Chen, Tzu-Yuan Tai, Kasumi Suzuki, Yi-Xuan Wang, Yun-Wei Lin","doi":"10.1159/000540867","DOIUrl":"10.1159/000540867","url":null,"abstract":"<p><strong>Introduction: </strong>Osimertinib (AZD9291) is a third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor that has shown significant clinical benefits in patients with EGFR-sensitizing mutations or the EGFR T790M mutation. The homologous recombination (HR) pathway is crucial for repairing DNA double-strand breaks (DSBs). Rad51 plays a central role in HR, facilitating the search for homology and promoting DNA strand exchange between homologous DNA molecules. Rad51 is overexpressed in numerous types of cancer cells. B02, a specific small molecule inhibitor of Rad51, inhibits the DNA strand exchange activity of Rad51. Previous studies have indicated that B02 disrupted Rad51 foci formation in response to DNA damage and inhibited DSBs repair in human cells and sensitized them to chemotherapeutic drugs in vitro and in vivo. However, the potential therapeutic effects of combining osimertinib with a Rad51 inhibitor are not well understood. The aim of this study was to elucidate whether the downregulation of Rad51 expression and activity can enhance the osimertinib-induced cytotoxicity in non-small cell lung cancer (NSCLC) cells.</p><p><strong>Methods: </strong>We used the MTS, trypan blue dye exclusion and colony-formation ability assay to determine whether osimertinib alone or in combination with B02 had cytotoxic effects on NSCLC cell lines. Real-time polymerase chain reaction was conducted to measure the amounts of Rad51 mRNA. The protein levels of phosphorylated AKT and Rad51 were determined by Western blot analysis.</p><p><strong>Results: </strong>We found that osimertinib reduced Rad51 expression by inactivating AKT activity. Rad51 knockdown using small interfering RNA or AKT inactivation through the phosphatidylinositol 3-kinase inhibitor LY294002 or si-AKT RNA transfection enhanced the cytotoxic and growth inhibitory effects of osimertinib. In contrast, AKT-CA (a constitutively active form of AKT) vector-enforced expression could mitigate the cytotoxic and cell growth inhibitory effects of osimertinib. Furthermore, B02 significantly enhanced the cytotoxic and cell growth inhibitory effects of osimertinib in NSCLC cells. Compared to parental cells, the activation of AKT and Rad51 expression in osimertinib-resistant cells could not be significantly inhibited by osimertinib treatment. Moreover, the increased expression of Rad51 is associated with the resistance mechanism in osimertinib-resistant H1975 and A549 cells.</p><p><strong>Conclusion: </strong>Collectively, the downregulation of Rad51 expression and activity enhances the cytotoxic effect of osimertinib in human NSCLC cells.</p>","PeriodicalId":10047,"journal":{"name":"Chemotherapy","volume":" ","pages":"12-25"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}