{"title":"On the occurrence of diphosphoinositol in the lipids of liver and pancreas","authors":"George A. Kfoury, Stanley E. Kerr","doi":"10.1016/0926-6542(64)90003-4","DOIUrl":"10.1016/0926-6542(64)90003-4","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The lipids of liver were reexamined for polyphosphoinositide, previously reported absent. The presence of a diphosphoinositide (DPI) was revealed by the isolation of inositol diphosphate (IP<sub>2</sub>) from an acid hydrolysate, and its glyceryl derivative from an alkaline hydrolysate of the concentrated phospholipid fraction.</p></span></li><li><span>2.</span><span><p>2. Starting with lipids extracted from liver and pancreas by azeotropic dehydration with 1,2-dichloroethane, materials soluble in acetone and alcohol were removed. After partition of the alcohol-insoluble phospholipids in CCl<sub>4</sub>-light petroleum-methanol-H<sub>2</sub>O (31:31:35:3, V/V), the non-polar fraction was assayed for inositol polyphosphate by chromatographic fractionation of the acid hydrolysate on Dowex-2 X8 Cl<sup>−</sup> A diphosphate mixture eluted by NaCl or LiCl was separated by refractionation with a formate system yielding IP<sub>2</sub> and glycerol diphosphate.</p></span></li><li><span>3.</span><span><p>3. The mixed glyceryl derivatives of IP<sub>2</sub> and glycerol diphosphate were secured by chromatographic fractionation of the alkaline hydrolysate. Based on the recovery of IP<sub>2</sub> and its glyceryl derivative, the content of DPI in the original pork liver lipid is 0.28 mmoles/kg.</p></span></li><li><span>4.</span><span><p>4. DPI could not be extracted by ether from acetone-dehydrated fresh beef liver but was extracted with neutral chloroform-methanol (2:1, V/V).</p></span></li><li><span>5.</span><span><p>5. Most of the IP<sub>2</sub> found in pacreatic lipid occured in material insoluble in CHCl<sub>3</sub> and in the CCl<sub>4</sub>-light petroleum solvent.</p></span></li></ul></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 391-403"},"PeriodicalIF":0.0,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90003-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23782909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comparison of the polyphosphoinositide in human and ox brain","authors":"Stanley E. Kerr, George A. Kfoury, Fuad S. Haddad","doi":"10.1016/0926-6542(64)90011-3","DOIUrl":"10.1016/0926-6542(64)90011-3","url":null,"abstract":"","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 461-463"},"PeriodicalIF":0.0,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90011-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23782917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Purification of bovine brain white matter poteolipids by dialysis in organic solvents","authors":"Marjorie B. Lees , Sheila Carr , Jordi Folch","doi":"10.1016/0926-6542(64)90012-5","DOIUrl":"10.1016/0926-6542(64)90012-5","url":null,"abstract":"","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 464-466"},"PeriodicalIF":0.0,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90012-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23782918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The site of action of phospholipase A on β-lecithins","authors":"G.H. De Haas, L.L.M. Van Deenen","doi":"10.1016/0926-6542(64)90014-9","DOIUrl":"10.1016/0926-6542(64)90014-9","url":null,"abstract":"","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 469-471"},"PeriodicalIF":0.0,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90014-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23782920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Die Fettsäuren des Fettes Mutterkorn (Claviceps purpurea)","authors":"O.W. Thiele","doi":"10.1016/0926-6542(64)90019-8","DOIUrl":"10.1016/0926-6542(64)90019-8","url":null,"abstract":"","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 483-486"},"PeriodicalIF":0.0,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90019-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73598006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Studies on the ganglioside micelle","authors":"Robert E. Howard, Robert Main Burton","doi":"10.1016/0926-6542(64)90007-1","DOIUrl":"10.1016/0926-6542(64)90007-1","url":null,"abstract":"<div><p>The molecular weight of β-ganglioside was measured by vapor pressure depression in a number of different solvents. The molecular weight was found to be near 1665, corresponding to a structure containing sphingosine, stearic acid, glucose, galactose, <em>N</em>-acetylgalactosamine, and two <em>N</em>-acetylneuraminic acid residues. In aqueous solution, the ganglioside associated to form a micelle structure with an aggregate weight of 200 000 or greater. The critical micelle concentration was found to be 1.0 · 10<sup>−5</sup> M. A discussion of these properties is presented.</p></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 435-440"},"PeriodicalIF":0.0,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90007-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23782913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Robert Main Burton, Robert E. Howard, Stanley Baer, Yvonna M. Balfour
{"title":"Gangliosides and acetylcholine of the central nervous system","authors":"Robert Main Burton, Robert E. Howard, Stanley Baer, Yvonna M. Balfour","doi":"10.1016/0926-6542(64)90008-3","DOIUrl":"10.1016/0926-6542(64)90008-3","url":null,"abstract":"<div><p>Both gangliosides are bound acetycholine are higher in grey matter than in white. The subcellular distributions of these two compounds appear to be parallel in rat brain, occuring primarily in the crude mitochondrial fraction, and upon further fractionation both gangliosides and bound acetycholine appear in the pinched-off nerve ending fraction. The synaptic vesicle isolated after disruption of the nerve endings by osmotic shock contains both acetylcholine and gangliosides. This parallel distribution and the physical properties of gangliosides suggest a functional role for gangliosides in the transport of acetylcholine from synaptic vesicles through the presynaptic membrane.</p></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 441-447"},"PeriodicalIF":0.0,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90008-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23782914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Localization of fatty acid synthesis in lactating mammary gland","authors":"W.C. Hülsmann, Dorothy S. Dow","doi":"10.1016/0926-6542(64)90020-4","DOIUrl":"10.1016/0926-6542(64)90020-4","url":null,"abstract":"","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 486-489"},"PeriodicalIF":0.0,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90020-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23782925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Die “Freien Lipide” aus Brucella abortus Bang","authors":"W Wober , O.W Thiele , B Urbaschek","doi":"10.1016/0926-6542(64)90002-2","DOIUrl":"10.1016/0926-6542(64)90002-2","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. <em>Brucella abortus</em> Bang was grown aerobically on glycerol-thionin-agar. The “free lipids” extracted from these bacteria (yielding 1.% of dry wt.) inhibit the Schultz-Dale reaction in the brucellose system.</p></span></li><li><span>2.</span><span><p>2. The lipid mixture was separated by acetone yieldng 81.2% acetone-insoluble fraction.</p></span></li><li><span>3.</span><span><p>3. The acetone-insoluble part was separated by column chromatography under thin-layer chromatographic control. According to appearing or disappearing of a component in the thin-layer chromatogram the fractions obtained by column chromatography were combined to form 30 collective fractions. Each of them consists of several components.</p></span></li><li><span>4.</span><span><p>4. By preparative thin-layer chromatography 7 aminophosphatides were isolated from the column-chromatographic fractions.</p></span></li><li><span>5.</span><span><p>5. Among the 7 phosphatides phosphatidylethanolamine, phosphatidylserine, lecithin and lysolecithin were identified. One of the remaining 3 phosphatides contains ethanolamine, two contain choline among their hydrolysis products. Moreover, they contain phosphate esters other than glycerophosphate.</p></span></li><li><span>6.</span><span><p>6. Among the component fatty acids of the 7 phosphatides saturated acids predominate, especially palmitic acid. Monoethenoic acids and octadecadienoic acid are also present. There are also odd-numbered straight-chain (<em>n</em>-C<sub>15</sub>, <em>n</em>-C<sub>17</sub>, <em>n</em>-C<sub>19</sub>) and hydroxy fatty acids in low concentrations and perhaps saturated methyl-branched C<sub>17</sub>, C<sub>19</sub> and C<sub>21</sub> acids. All phosphatides contain C<sub>19</sub>-cyclopropane acid which was reported recently.</p></span></li></ul></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 376-390"},"PeriodicalIF":0.0,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90002-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78990528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The occurrence and metabolism In vitro of unesterified fatty acid in mouse brain","authors":"C.E. Rowe","doi":"10.1016/0926-6542(64)90006-X","DOIUrl":"10.1016/0926-6542(64)90006-X","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. A method has been developed for the isolation and estimation of unesterified fatty acid in tissue extracts. This is based on treatment of extracted lipid with diazomethane followed by isolation of the methyl esters by chromatography on a thin layer of alumina.</p></span></li><li><span>2.</span><span><p>2. It has been found that mouse brain contains appreciable quantities of unesterified fatty acid.</p></span></li><li><span>3.</span><span><p>3. The principal acids were palmitic, stearic, oleic and arachidonic acids.</p></span></li><li><span>4.</span><span><p>4. When mouse brain was incubated with sodium [I-<sup>14</sup>C]acetate for periods up to 6 h, 28%–66% of the incorporation into lipid was into unesterified fatty acid.</p></span></li><li><span>5.</span><span><p>5. Of the principal acids acetate was incorporated into palmitic acid only.</p></span></li></ul></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 424-434"},"PeriodicalIF":0.0,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90006-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23782912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}