Die “Freien Lipide” aus Brucella abortus Bang

• 1.

  1. Brucella abortus Bang was grown aerobically on glycerol-thionin-agar. The “free lipids” extracted from these bacteria (yielding 1.% of dry wt.) inhibit the Schultz-Dale reaction in the brucellose system.

• 2.

  2. The lipid mixture was separated by acetone yieldng 81.2% acetone-insoluble fraction.

• 3.

  3. The acetone-insoluble part was separated by column chromatography under thin-layer chromatographic control. According to appearing or disappearing of a component in the thin-layer chromatogram the fractions obtained by column chromatography were combined to form 30 collective fractions. Each of them consists of several components.

• 4.

  4. By preparative thin-layer chromatography 7 aminophosphatides were isolated from the column-chromatographic fractions.

• 5.

  5. Among the 7 phosphatides phosphatidylethanolamine, phosphatidylserine, lecithin and lysolecithin were identified. One of the remaining 3 phosphatides contains ethanolamine, two contain choline among their hydrolysis products. Moreover, they contain phosphate esters other than glycerophosphate.

• 6.

  6. Among the component fatty acids of the 7 phosphatides saturated acids predominate, especially palmitic acid. Monoethenoic acids and octadecadienoic acid are also present. There are also odd-numbered straight-chain (n-C₁₅, n-C₁₇, n-C₁₉) and hydroxy fatty acids in low concentrations and perhaps saturated methyl-branched C₁₇, C₁₉ and C₂₁ acids. All phosphatides contain C₁₉-cyclopropane acid which was reported recently.