Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects最新文献_第8页

Le role l'acide ascorbique dans l'oxydation enzymatique du diphosphopyridine nucléotide réduit 抗坏血酸在酶氧化还原二磷酸吡啶核苷酸中的作用

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 Epub Date: 2003-02-05 DOI: 10.1016/0926-6569(64)90283-4

Etienne Gero

引用次数: 5

Purification and characterization of ribonuclease from rabbit reticulocytes 兔网织细胞核糖核酸酶的纯化及特性研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 Epub Date: 2003-02-05 DOI: 10.1016/0926-6569(64)90269-X

Kenji Adachi, Kei Nagano, Toshiko Nakao, Makoto Nakao

{"title":"Purification and characterization of ribonuclease from rabbit reticulocytes","authors":"Kenji Adachi, Kei Nagano, Toshiko Nakao, Makoto Nakao","doi":"10.1016/0926-6569(64)90269-X","DOIUrl":"10.1016/0926-6569(64)90269-X","url":null,"abstract":"<div>A method is described for the purification of ribonmuclease (EC 2.7.7.16) from rabbit reticulocytes by heart treatment at pH 2.5 followed by chromatography on a carboxymethylcellulose column. The enzymei was purified 5000-fold, and was free from both acid and alkaline phosphatases (EC 3.I.3.2 and EC 3.I.3.I, respectively), non-specific phosphodiesterase (EC 3.I.4.I) and deoxyribonuclease (EC 3.I.4.5).The enzyme had a pH optimum at 5.8. It was heat and acid stable and could be stored at —20° for I year without loss of activity. The products of hydrolysis of yeast ribonucleic acid by the enzyme were found to contain both 2′- and 3′-purine and pyrimide nucleotides,t he ratio of ratio of adenylic to cytidylic acid being approximately I:I. An incomplete digest of yeast ribonucleic acid by the enzyme was separated on a diethylaminoethyl-cellulose column and both mono- and oligonucleotide fractions were identified. Nucleoside 2′- and 3′-cyclic phosphates were hydrolyzed to their corresponding non-cyclic mononucleotides by the enzyme.A possible physiological role of the enzyme is discussed on the basis of the following experiments: (I) degradation of rabbit reticulocyte ribosome by the enzyme; (2) chronological difference in its activity in rabbit reticulocytes; and (3) intracellular localization of the enzyme.</div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 1","pages":"Pages 59-70"},"PeriodicalIF":0.0,"publicationDate":"1964-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90269-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23793357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The metabolism of l-rhamnose in Escherichia coli l-鼠李糖在大肠杆菌中的代谢

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 Epub Date: 2003-02-05 DOI: 10.1016/0926-6569(64)90264-0

Yasuyuki Takagi , Hideo Sawada

引用次数: 29

Luminescence properties of muramidase and reoxidized muramidase 酶酰胺酶和再氧化酶的发光性质

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 Epub Date: 2003-02-05 DOI: 10.1016/0926-6569(64)90295-0

Jorge E. Churchich

引用次数: 11

Structure primaire d'un inhibiteur pancréatique de la trypsine (inhibiteur de Kunitz et Northrop) 胰腺胰蛋白酶抑制剂(Kunitz和Northrop抑制剂)的一级结构

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 Epub Date: 2003-02-05 DOI: 10.1016/0926-6569(64)90297-4

J. Chauvet, G. Nouvel, R. Archer

引用次数: 0

The metabolism of l-rhamnose in escherichia coli l-鼠李糖在大肠杆菌中的代谢

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 Epub Date: 2003-02-05 DOI: 10.1016/0926-6569(64)90263-9

Yasuyuki Takagi , Hideo Sawada

引用次数: 53

Purification and chemical characterization of alanine dehydrogenase of Bacillus subtilis 枯草芽孢杆菌丙氨酸脱氢酶的纯化及化学性质研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 Epub Date: 2003-02-05 DOI: 10.1016/0926-6569(64)90266-4

Akira Yoshida, Ernst Freese

{"title":"Purification and chemical characterization of alanine dehydrogenase of Bacillus subtilis","authors":"Akira Yoshida, Ernst Freese","doi":"10.1016/0926-6569(64)90266-4","DOIUrl":"10.1016/0926-6569(64)90266-4","url":null,"abstract":"<div><ul><li>1.1. Bacillus subtilis alanine dehydrogenase (L-alanine: NAD oxidoreductase (deaminating), EC1.4.1.1. has been purified by column chromatography using calcium phosphate gel, DEAE-Sephadex and Ecteola-cellulose; it has been obtained in crystalline form.</li><li>2.2. The sedimentation pattern indicated a homogenous preparation. The sedimentation constant at infinite dilution was 8.8 S. The molecular weight estimated by the sedimentation equilibrium method was 228 000.</li><li>3.3. Amino acid analysis gave the following ratios of amino acid residues: Asp, 43; Thr, 34; Ser, 20; Glu, 51; Pro, 25; Gly, 51; Ala, 65; CySH, 2; Val, 49; Met, 11; iLeu, 28; Leu, 42; Tyr, 15; Phe, 7; Try, 1; Lys, 28; His, 9; Arg, 14.</li></ul></div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 1","pages":"Pages 33-43"},"PeriodicalIF":0.0,"publicationDate":"1964-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90266-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23793354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60

Régulations métaboliques de la biosynthèse de la méthionine et de la thréonine chez Saccharomyces cerevisiae 酿酒酵母中蛋氨酸和苏氨酸生物合成的代谢调节

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 Epub Date: 2003-02-05 DOI: 10.1016/0926-6569(64)90262-7

H. De Robichon-Szulmajster, D. Corrivaux

引用次数: 10

Phosphoglucomutase: A model for active0site characterization II. Optical rotatory dispersion studies 磷酸葡萄糖糖化酶:活性位点表征的模型II。旋光色散研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 Epub Date: 2003-02-05 DOI: 10.1016/0926-6569(64)90287-1

N.H. Sloane

引用次数: 4

Reconstitution of respiratory chain enzyme systems. XV. Reconstitution of succinate oxidase using soluble succinate dehydrogenase and a “cyanide particle” 呼吸链酶系统的重构。十五。利用可溶性琥珀酸脱氢酶和“氰化物颗粒”重构琥珀酸氧化酶

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 Epub Date: 2003-02-05 DOI: 10.1016/0926-6569(64)90288-3

David F. Wilson , Tsoo E. King

引用次数: 8