Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects最新文献_第8页

Purification and chemical characterization of alanine dehydrogenase of Bacillus subtilis 枯草芽孢杆菌丙氨酸脱氢酶的纯化及化学性质研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI: 10.1016/0926-6569(64)90266-4

Akira Yoshida, Ernst Freese

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引用次数: 60

Purification and characterization of ribonuclease from rabbit reticulocytes 兔网织细胞核糖核酸酶的纯化及特性研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI: 10.1016/0926-6569(64)90269-X

Kenji Adachi, Kei Nagano, Toshiko Nakao, Makoto Nakao

{"title":"Purification and characterization of ribonuclease from rabbit reticulocytes","authors":"Kenji Adachi, Kei Nagano, Toshiko Nakao, Makoto Nakao","doi":"10.1016/0926-6569(64)90269-X","DOIUrl":"10.1016/0926-6569(64)90269-X","url":null,"abstract":"<div>A method is described for the purification of ribonmuclease (EC 2.7.7.16) from rabbit reticulocytes by heart treatment at pH 2.5 followed by chromatography on a carboxymethylcellulose column. The enzymei was purified 5000-fold, and was free from both acid and alkaline phosphatases (EC 3.I.3.2 and EC 3.I.3.I, respectively), non-specific phosphodiesterase (EC 3.I.4.I) and deoxyribonuclease (EC 3.I.4.5).The enzyme had a pH optimum at 5.8. It was heat and acid stable and could be stored at —20° for I year without loss of activity. The products of hydrolysis of yeast ribonucleic acid by the enzyme were found to contain both 2′- and 3′-purine and pyrimide nucleotides,t he ratio of ratio of adenylic to cytidylic acid being approximately I:I. An incomplete digest of yeast ribonucleic acid by the enzyme was separated on a diethylaminoethyl-cellulose column and both mono- and oligonucleotide fractions were identified. Nucleoside 2′- and 3′-cyclic phosphates were hydrolyzed to their corresponding non-cyclic mononucleotides by the enzyme.A possible physiological role of the enzyme is discussed on the basis of the following experiments: (I) degradation of rabbit reticulocyte ribosome by the enzyme; (2) chronological difference in its activity in rabbit reticulocytes; and (3) intracellular localization of the enzyme.</div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 1","pages":"Pages 59-70"},"PeriodicalIF":0.0,"publicationDate":"1964-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90269-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23793357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The metabolism of l-rhamnose in escherichia coli l-鼠李糖在大肠杆菌中的代谢

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI: 10.1016/0926-6569(64)90263-9

Yasuyuki Takagi , Hideo Sawada

引用次数: 53

The metabolism of l-rhamnose in Escherichia coli l-鼠李糖在大肠杆菌中的代谢

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI: 10.1016/0926-6569(64)90264-0

Yasuyuki Takagi , Hideo Sawada

引用次数: 29

Luminescence properties of muramidase and reoxidized muramidase 酶酰胺酶和再氧化酶的发光性质

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI: 10.1016/0926-6569(64)90295-0

Jorge E. Churchich

引用次数: 11

Le role l'acide ascorbique dans l'oxydation enzymatique du diphosphopyridine nucléotide réduit 抗坏血酸在酶氧化还原二磷酸吡啶核苷酸中的作用

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI: 10.1016/0926-6569(64)90283-4

Etienne Gero

引用次数: 5

Structure primaire d'un inhibiteur pancréatique de la trypsine (inhibiteur de Kunitz et Northrop) 胰腺胰蛋白酶抑制剂(Kunitz和Northrop抑制剂)的一级结构

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI: 10.1016/0926-6569(64)90297-4

J. Chauvet, G. Nouvel, R. Archer

引用次数: 0

Studies on the repression of β-galactosidase in Escherichia coli 大肠杆菌中β-半乳糖苷酶抑制的研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI: 10.1016/0926-6569(64)90272-X

David J. Clark , Allen G. Marr

引用次数: 20

The chemical reactivity of the thiol group in the active centre of ficin 苯酚活性中心巯基的化学反应活性

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI: 10.1016/0926-6569(64)90275-5

M.R. Hollaway, A.P. Mathias, B.R. Rabin

{"title":"The chemical reactivity of the thiol group in the active centre of ficin","authors":"M.R. Hollaway, A.P. Mathias, B.R. Rabin","doi":"10.1016/0926-6569(64)90275-5","DOIUrl":"10.1016/0926-6569(64)90275-5","url":null,"abstract":"<div><ul><li>1.1. The irreversible inhibition of ficin (EC 3.4.4.12) by chloroacetamide or iodoacetamide is due to the alkylation of the thiol group of one particular cysteine residue in the enzyme.</li><li>2.2. The variation of the rate of alkylation of ficin with pH shows that the reactive thiol group of ficin behaves essenitally as a freely ionising roup, pKa′ = 8.55 (25 °; I, 0.1). Hence it is concluded that this group is unlikely to exist in a hydrogen-bonded form in the free enzyme.</li><li>3.3. The rates of reaction of ficin with chloroacetamide or iodacetamide were accelerated in the presence of substrates or products of catalytic activity without the stoichiometry of the overall reaction being affected. The rate of addition of the essential thiol of ficin to N-ethylmaleimide was unaffected by substrate or substrate analogues.</li><li>4.4. The nucleophilicity of the essential thiol group of ficin in SN2 reactions was found to be greater than that of simple thiol compounds whereas in the addition reaction to N-ethylmaleimide the converse was found.</li><li>5.5. A possible reason for the high nucleophilicyt of ficin in SN2 reactions and the acceleration caused by the presence of substrate or substrate analogues is given.</li></ul></div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 1","pages":"Pages 111-124"},"PeriodicalIF":0.0,"publicationDate":"1964-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90275-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23795481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Effect of monovalent cations on the adenosinetriphosphatase of sonicated erythrocyte membrane 一价阳离子对超声红细胞膜腺苷三磷酸酶的影响

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI: 10.1016/0926-6569(64)90277-9

Amir Askari, Joseph C. Fratantoni

{"title":"Effect of monovalent cations on the adenosinetriphosphatase of sonicated erythrocyte membrane","authors":"Amir Askari, Joseph C. Fratantoni","doi":"10.1016/0926-6569(64)90277-9","DOIUrl":"10.1016/0926-6569(64)90277-9","url":null,"abstract":"<div><ul><li>1.1. Human erythrocyte membrane contains a Mg2+-dependent adenosinetriphosphatase activity. The enhancement of this activity requires the simultaneous presence of Na+ and K+. When the membranes were broken by ultrasonic vibrations, the adenosinetriphosphatase activity was enhanced with either Na+ and K+, and in this case enhancement was no longer affected by ouabain.</li><li>2.2. The effects of Mg2+, Ca2+, pH, and detergents on the various manifestations of the adenosinetriphosphatase activity, namely: (a) the Na+-plus-K+-dependent form, (b) the single-ion-dependent form, and (c) the ion-independent form, were studied. The results did not suggest the presence of more than one enzyme.</li><li>3.3. Since the single-ion-dependent adenosinetriphosphatase activity was revealed merely by a physical change in the method of preparation (sonication), it is reasonable to suspect that it and the Na+-plus-K+-dependent activity are different manifestations of a single enzymic activity.</li></ul></div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 1","pages":"Pages 132-141"},"PeriodicalIF":0.0,"publicationDate":"1964-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90277-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23795483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24