{"title":"枯草芽孢杆菌丙氨酸脱氢酶的纯化及化学性质研究","authors":"Akira Yoshida, Ernst Freese","doi":"10.1016/0926-6569(64)90266-4","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. <em>Bacillus subtilis</em> alanine dehydrogenase (<span>L</span>-alanine: NAD oxidoreductase (deaminating), EC<sub>1.4.1.1.</sub> has been purified by column chromatography using calcium phosphate gel, DEAE-Sephadex and Ecteola-cellulose; it has been obtained in crystalline form.</p></span></li><li><span>2.</span><span><p>2. The sedimentation pattern indicated a homogenous preparation. The sedimentation constant at infinite dilution was 8.8 S. The molecular weight estimated by the sedimentation equilibrium method was 228 000.</p></span></li><li><span>3.</span><span><p>3. Amino acid analysis gave the following ratios of amino acid residues: Asp, <sub>43</sub>; Thr, <sub>34</sub>; Ser, <sub>20</sub>; Glu, <sub>51</sub>; Pro, <sub>25</sub>; Gly, <sub>51</sub>; Ala, <sub>65</sub>; CySH, <sub>2</sub>; Val, <sub>49</sub>; Met, <sub>11</sub>; iLeu, <sub>28</sub>; Leu, <sub>42</sub>; Tyr, <sub>15</sub>; Phe, <sub>7</sub>; Try, <sub>1</sub>; Lys, <sub>28</sub>; His, <sub>9</sub>; Arg, <sub>14</sub>.</p></span></li></ul></div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 1","pages":"Pages 33-43"},"PeriodicalIF":0.0000,"publicationDate":"1964-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90266-4","citationCount":"60","resultStr":"{\"title\":\"Purification and chemical characterization of alanine dehydrogenase of Bacillus subtilis\",\"authors\":\"Akira Yoshida, Ernst Freese\",\"doi\":\"10.1016/0926-6569(64)90266-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. <em>Bacillus subtilis</em> alanine dehydrogenase (<span>L</span>-alanine: NAD oxidoreductase (deaminating), EC<sub>1.4.1.1.</sub> has been purified by column chromatography using calcium phosphate gel, DEAE-Sephadex and Ecteola-cellulose; it has been obtained in crystalline form.</p></span></li><li><span>2.</span><span><p>2. The sedimentation pattern indicated a homogenous preparation. The sedimentation constant at infinite dilution was 8.8 S. The molecular weight estimated by the sedimentation equilibrium method was 228 000.</p></span></li><li><span>3.</span><span><p>3. Amino acid analysis gave the following ratios of amino acid residues: Asp, <sub>43</sub>; Thr, <sub>34</sub>; Ser, <sub>20</sub>; Glu, <sub>51</sub>; Pro, <sub>25</sub>; Gly, <sub>51</sub>; Ala, <sub>65</sub>; CySH, <sub>2</sub>; Val, <sub>49</sub>; Met, <sub>11</sub>; iLeu, <sub>28</sub>; Leu, <sub>42</sub>; Tyr, <sub>15</sub>; Phe, <sub>7</sub>; Try, <sub>1</sub>; Lys, <sub>28</sub>; His, <sub>9</sub>; Arg, <sub>14</sub>.</p></span></li></ul></div>\",\"PeriodicalId\":100170,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects\",\"volume\":\"92 1\",\"pages\":\"Pages 33-43\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-10-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6569(64)90266-4\",\"citationCount\":\"60\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926656964902664\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926656964902664","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Purification and chemical characterization of alanine dehydrogenase of Bacillus subtilis
1.
1. Bacillus subtilis alanine dehydrogenase (L-alanine: NAD oxidoreductase (deaminating), EC1.4.1.1. has been purified by column chromatography using calcium phosphate gel, DEAE-Sephadex and Ecteola-cellulose; it has been obtained in crystalline form.
2.
2. The sedimentation pattern indicated a homogenous preparation. The sedimentation constant at infinite dilution was 8.8 S. The molecular weight estimated by the sedimentation equilibrium method was 228 000.
3.
3. Amino acid analysis gave the following ratios of amino acid residues: Asp, 43; Thr, 34; Ser, 20; Glu, 51; Pro, 25; Gly, 51; Ala, 65; CySH, 2; Val, 49; Met, 11; iLeu, 28; Leu, 42; Tyr, 15; Phe, 7; Try, 1; Lys, 28; His, 9; Arg, 14.
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