化学最新文献

{"title":"Femtomolar-level detection of procalcitonin using a split aptamer-based fiber optic nanogold-linked sorbent assay for diagnosis of sepsis.","authors":"Yen-Ta Tseng, Yi-Hyuan Yu, Ying-Yi Yeh, Phuong Chi Mai, Tze-Ta Huang, Chun-Jen Huang, Lai-Kwan Chau, Yuh-Ling Chen","doi":"10.1016/j.talanta.2025.128150","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128150","url":null,"abstract":"<p><p>Sepsis requires very sensitive and rapid detection of biomarkers to guide antibiotic therapy to lower the patient mortality rate. Here, we report an ultrasensitive, rapid, and low-cost method for detection of the most popular sepsis biomarker, procalcitonin (PCT), based on a split aptamer-based fiber optic nanogold-linked sorbent assay. After successful development of a novel aptamer for PCT by SELEX, a pair of split aptamer fragments from the parent aptamer was predicted through molecular interaction simulation via free webservers. Then one fragment (AptF1) is modified on a sensor fiber and the other fragment (AptF2) is conjugated to a gold nanoparticle (AuNP@AptF2). The interactions among PCT and the two aptamer fragments lead to the formation of a AuNP@AptF2-PCT-AptF1 sandwich-like nanocomplex on the fiber core surface, leading to strong nanoplasmonic absorption near the fiber core surface. This method provides a linear response ranging from 500 fg/mL to 5.0 ng/mL and a detection limit of 50 fg/mL (3.9 fM). The method had been validated against the clinical accepted electrochemiluminescence immunoassay using clinical samples and very good correlation between the results by both methods was found. Therefore, this method offers a potential for point-of-care diagnosis of sepsis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128150"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-platinum nanozyme-mediated ratiometric fluorescent immunosensor with dual catalytic cascades for sensitive detection of fenitrothion.","authors":"Jie Liu, Hao Deng, Qing-Chun Yin, Min-Fu Wu, Lin Luo, Hong Wang, Zhen-Lin Xu, Bao-Zhu Jia","doi":"10.1016/j.talanta.2025.128162","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128162","url":null,"abstract":"<p><p>Organophosphorus pesticide residues pose significant threats to food safety and environmental health, and their monitoring methods demand sensitivity and anti-interference capability. In this study, we developed a novel ratiometric fluorescent immunosensor for fenitrothion (FN) detection by employing chitosan-modified platinum nanoparticles (Ch-Pt NPs) as catalytic nanozymes. Ch-Pt NPs exhibit oxidase-mimicking activity, enabling the oxidation of ascorbic acid (AA) and o-phenylenediamine (OPD) to produce dehydroascorbic acid (DHAA) and 2,3-diaminophenazine (DAP, Em = 568 nm), respectively. Then, DHAA further reacted with the remaining OPD to generate quinoxaline derivatives (DFQ, Em = 430 nm). These two products further form ratiometric fluorescent signals. Taking AA as a breakthrough point, the dual-catalytic cascade of natural enzymes and nanozymes was achieved by the combination with the alkaline phosphatase (ALP) -based enzyme-linked immunosorbent assay (ALP-ELISA). Compared with the conventional colorimetric ELISA, the proposed sensor demonstrated a nearly 20-fold enhancement in sensitivity (detection limit: 0.48 ng/mL) and achieved satisfactory recoveries of 80.0-108.3 % in spiked samples. The ratiometric fluorescent immunosensor demonstrates outstanding performance and holds great potential for extensive applications in the field of pesticide monitoring.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128162"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An AIE fluorescence probe for ratiometric and selective detection of Sn²⁺ based on proton transfer.","authors":"Duo-Duo Hu, Guangnan Wei, Ping Yang, Dexin Fu, Kuiliang Li","doi":"10.1016/j.talanta.2025.128078","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.128078","url":null,"abstract":"<p><p>Detecting Sn²⁺ heavy metal ions is crucial for mitigating water pollution and safeguarding human health. This study involved the design and synthesis of a novel fluorescence probe, RTPE-IM, which is a combination of an aggregation-induced emission luminophore (TPE) and rhodamine B. The probe undergoes proton transfer in the presence of Sn²⁺ ion, displaying high selectivity and a low detection limit of 0.121 μM. The synthesis and application of this molecule offer a potential strategy for preventing environmental health risks.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128078"},"PeriodicalIF":5.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode sensor innovation based on light-harvesting and nanozyme integrated Cu-MOF loaded Cu₂O label.","authors":"Man Xu, Zheng Wang, Yingzhuo Shen, Meijuan Zhao, Xiaoya Hu, Hongbo Li, Wei Liu, Qin Xu","doi":"10.1016/j.talanta.2025.128012","DOIUrl":"10.1016/j.talanta.2025.128012","url":null,"abstract":"<p><p>Integrating photoelectrochemical (PEC) and colorimetric dual-mode shed light on \"all-in-one\" sensing platform for high sensitivity, in situ visualization and self-validating measurement. Its attainment depends on the capabilities of light harvesting and colorimetric signal generation from bi-functional tags, which remains challenging. Herein, Cu-MOF loaded Cu₂O (Cu₂O@Cu-MOF) was deliberately prepared and employed as the PEC- colorimetric signal reporter with tail-made stable photo-current and peroxidase mimic activity. A thin layer of Zr-MOF was deliberately encapsulated onto Cu₂O@Cu-MOF for the immobilization of aptamer (Apt) which can be captured by the magnetic AuNPs/Fe₃O₄ nanoparticles through duplex-specific hybrid. In the presence of target analyte, PEC- colorimetric signal reporter was quantitatively released from the magnetic AuNPs/Fe₃O₄ after one-step convenient magnetic separation, facilitating photocurrent response by the photo-induced excitation and colorimetric response by catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB). In a proof-of-concept experiment, a customized PEC- colorimetric dual-mode sensor platform based on Cu₂O@Cu-MOF reporter has achieved an ultrasensitive response for bisphenol A (BPA), exhibiting sub-picomole (PEC signal) and sub-nanomole (colorimetric signal) detection limits, respectively. Our dual-mode sensor platform strategy aims to extend to high-sensitivity and visual analysis in fields of in vitro diagnosis, environmental monitoring, and food safety.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"128012"},"PeriodicalIF":5.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drain-Less Submandibular Gland Excision With Preserved Facial Artery.","authors":"Ahmed Nofal, Mohammad Waheed El-Anwar, Mohamed A Al Shawadfy, Yasser Ahmed Fouad","doi":"10.1177/01455613221142735","DOIUrl":"10.1177/01455613221142735","url":null,"abstract":"<p><p>ObjectivesTo describe our experience in excision of the submandibular gland (SMG) without drain insertion and with preservation of the facial artery as a day case surgery as well as evaluation of the surgical outcomes of this procedure.MethodsProspective case series study of 42 cases of chronic calcular submandibular sialadenitis that underwent SMG excision by the same surgical team during the period from 2017 to 2021. The initial surgical plan in all cases was to excise the SMG with preservation of the facial artery and without drain insertion.ResultsSMG excision without drain insertion was successfully achieved in 28 patients who were discharged on the same day. In the remaining 14 patients, the surgical dissection was difficult and a suction drain was inserted at the end of the surgery; consequently, they were discharged on the next day. In all cases, facial vessels were preserved, and complete gland excision was achieved. Among the 28 patients who had no drain insertion, 1 patient had a postoperative seroma and no patient had wound related complications. Among the 14 patients who had drain insertion, 2 patients had postoperative seroma and 3 patients had wound related complications in the form of obvious scar formation. There were no other significant complications in all patients.ConclusionsSubmandibular gland (SMG) excision with facial artery preservation and without drain insertion as a day case surgery could be safely done in cases of chronic calcular inflammation provided that meticulous surgical dissection and complete hemostasis were achieved.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":" ","pages":"559-563"},"PeriodicalIF":17.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40714115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Giant Fronto-Ethmoidal Osteoma Presenting With Proptosis and Diplopia.","authors":"Derya Guclu, Emine Izgi, Elif N Unlu, Hayri Ogul","doi":"10.1177/01455613221141220","DOIUrl":"10.1177/01455613221141220","url":null,"abstract":"<p><p>Osteomas are benign bone tumors commonly involving paranasal sinus walls. They are divided into three groups as ivory, mature, and mixed form. We reported demonstrative radiological features of an unusual case of giant osteoma presenting with proptosis and diplopia.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":" ","pages":"NP572-NP574"},"PeriodicalIF":17.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40702797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tapia's Syndrome: Clinical Insights.","authors":"Ersilia Ruggiero, Alessandro Vergari, Fabio Sbaraglia, Michelangelo Mario Spanò, Rossano Festa, Marco Rossi","doi":"10.1177/01455613221140294","DOIUrl":"10.1177/01455613221140294","url":null,"abstract":"","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":" ","pages":"538-539"},"PeriodicalIF":17.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40482864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membranous Nasopharyngeal Stenosis: Case Report.","authors":"Derya Guclu, Elif N Unlu, Hayri Ogul","doi":"10.1177/01455613221142737","DOIUrl":"10.1177/01455613221142737","url":null,"abstract":"<p><p>Nasopharyngeal stenosis is a rare condition and occurs usually secondary to nasopharyngeal or oropharyngeal surgery and radiotherapy. It may cause obstructive symptoms in patients and is diagnosed usually with nasopharyngoscopy. Treatment is difficult and requires different surgical techniques including balloon dilatation. Even after a successful surgery, recurrence is very common.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":" ","pages":"543-544"},"PeriodicalIF":17.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40480370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of plastic blends based on virtual mid-infrared spectroscopy combined with chemometric methods.","authors":"Jian Yang, Yu-Peng Xu, Xiao-Li Chu","doi":"10.1016/j.talanta.2025.128006","DOIUrl":"10.1016/j.talanta.2025.128006","url":null,"abstract":"<p><p>Developing efficient and accurate quantitative analysis methods for plastic blends holds significant value for resource recycling and environmental monitoring. Mid-infrared (MIR) spectroscopy, combined with chemometric techniques, has demonstrated excellent performance in plastic blend quantification. However, obtaining mid-infrared spectral data for a large number of plastic blends to calibrate the model remains challenging. This study proposes an innovative approach that utilizes pure plastic MIR spectra and the Beer-Lambert law to generate virtual plastic blend spectra. Four experimental groups (A-D) were designed, incorporating both real and virtual spectra to systematically evaluate the method's effectiveness. Experiment group A established a baseline model using real spectra, while experiment groups B, C, and D respectively validated the applicability and generalization capability of models based on virtual spectra, as well as their potential applications in MIR hyperspectral imaging (MIR-HSI), respectively. The study further explores feature band selection, model construction, evaluation, and interpretation. The results demonstrate that this method can efficiently predict the mass percentages of components in ternary plastic blends. In experimental group C, partial least squares regression (PLSR), one-dimensional convolutional neural network (CNN1D), and two-dimensional convolutional neural network based on Gramian Angular Field (GAF-CNN2D) models-trained on 208 virtual plastic blend spectra-were employed to predict the mass percentages of 66 ternary plastic blends composed of polyethylene (PE), polypropylene (PP), and polystyrene (PS). The prediction coefficients of determination (R_T²) reached 0.9872, 0.9879, and 0.9944, respectively, indicating exceptional predictive accuracy. Experimental group D further demonstrated that, even under Gaussian noise interference and limited spectral range, the strategy of fusing mid-wave infrared and long-wave infrared bands allowed the PLSR and GAF-CNN2D models to maintain high performance in predicting the mass percentages of 66 ternary blends of PE, PP, and PS, with R_T² values of 0.9852 and 0.9895, respectively. This suggests that the proposed method holds potential for applications in MIR-HSI and is promising for real-time online analysis. Finally, this study proposes a more widely applicable and optimized quantitative analysis application scheme based on virtual plastic blend spectra, aiming to enable rapid and precise determination of unknown plastic blends.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"292 ","pages":"128006"},"PeriodicalIF":5.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Specific Selective Neck Dissection for Isolated Regional Failure in Nasopharyngeal Carcinoma after Radiation.","authors":"Jian-Hui Wu, Jian-Hui Xu, Fei Ye, Min-Yi Fu","doi":"10.1177/01455613221140280","DOIUrl":"10.1177/01455613221140280","url":null,"abstract":"<p><p>BackgroundTo assess whether specific selective neck dissection (SND) with involved levels is a feasible treatment for isolated regional failure in nasopharyngeal carcinoma (NPC) after radiation.Material and MethodsBetween January 2011 and December 2019, a total of 46 patients were assigned to undergo SND in the Department of Head and Neck Surgery at our center. The dissection extent of specific SND usually only involved levels of lymph node sites for isolated regional failure; in addition, lesions of level II or III involved removing both level II and III lymph nodes. The patients' clinical, MRI and pathological characteristics, overall survival (OS), disease-free survival (DFS) and regional-free survival (RFS) were evaluated and analyzed.ResultsLevel II was the most commonly involved cervical nodal region in 28 neck dissection specimens (54.9%), followed by level III with positive nodes in 11 specimens (21.6%). Eleven patients (34.8%) had post-SND locoregional recurrence without distant metastasis. Of the patients, 7 patients (30.4%) had regional recurrence, and only one patient (2.8%) had lymph node recurrence on the side of SND. In addition, 8 patients (17.4%) had post-SND distant metastasis. The OS, DFS, and RFS of the patients were 76.1%, 58.7%, and 69.6%, respectively, at 3 years. The OS, DFS, and RFS values of patients who underwent SND were similar to those of patients who underwent comprehensive neck dissection (CND) and/or SND in published articles.ConclusionSpecific SND was shown to be an effective and feasible treatment for isolated regional failure in NPC.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":" ","pages":"NP626-NP630"},"PeriodicalIF":17.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40684821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}