化学最新文献

{"title":"Structural characterization and antioxidant activity evaluation of a polysaccharide from pink Auricularia cornea.","authors":"Lei Ye, Yu Huang, Lingzi Zhang, Xin Li, Bo Zhang, Xuezhen Yang, Jianhua Luo, Hongping Liu, Xiaoping Zhang, Chuan Song, Zonghua Ao, Caihong Shen, Wei Tan, Xiaolin Li","doi":"10.1016/j.ijbiomac.2024.138149","DOIUrl":"10.1016/j.ijbiomac.2024.138149","url":null,"abstract":"<p><p>An acidic polysaccharose (YL-D2N2) was isolated from crude polysaccharides of pink Auricularia cornea and characterized for its structural and antioxidant properties. YL-D2N2 consists of fucose, galactose, glucose, xylose, mannose and glucuronic acid in a molar ratio of 0.85: 1.50: 4.44: 27.52: 46.56: 19.13. It has a number-average molecular weight of about 52.811 kDa and a weight-average molecular weight of about 135.457 kDa. Structural characterization showed that YL-D2N2 consists of nine residues (Xylp-(1→, GlcpA-(1→, →2)-Xylp-(1→, →3)-Galp-(1→, →3)-Manp-(1→, →4)-GlcpA-(1→, →2,3)-Manp-(1→, →3,4)-Glcp-(1→, →3,6)-Manp-(1→), with a backbone of →3)-β-D-Manp-(1→, →2,3)-α-D-Manp-(1→, →3,6)-α-D-Manp-(1 → and side chains containing β-D-Xylp-(1 → and α-D-GlcpA-(1→. Notably, YL-D2N2 exhibits significant radical scavenging activity for superoxide anions, reaching 50.82 ± 0.64 % at a concentration of 3.2 mg/mL. Overall, YL-D2N2 exhibits a unique chemical structure and specialized applications for targeting superoxide anion radicals, providing valuable insights for further exploration of its structure-activity relationship.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"284 Pt 1","pages":"138149"},"PeriodicalIF":7.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan/sodium alginate/ethyl cellulose-based multilayer film incorporated with l-ascorbic acid for improved barrier and antioxidant properties.","authors":"Yasir Abbas Shah, Saurabh Bhatia, Ahmed Al-Harrasi, Talha Shireen Khan","doi":"10.1016/j.ijbiomac.2024.138169","DOIUrl":"10.1016/j.ijbiomac.2024.138169","url":null,"abstract":"<p><p>A bioactive multilayer film (ML) loaded with l-Ascorbic acid (AA) was developed using chitosan (CH), sodium alginate (SA), and ethyl cellulose (EC). Various properties of the films, including morphological, hydrophobic, barrier, mechanical, optical, and antioxidant characteristics, were evaluated and compared to those of monolayer films made from each biopolymer. The cross-sectional analysis via scanning electron microscopy revealed the successful preparation of the ML film with layering of the different biopolymers. For the ML film the resulting water contact angle was observed with an average of 73.86° and the film showed water resistant properties as compared to the individual CH and SA films. The ML film showed the lowest water vapor transmission rate (WVTR) at 54.99 g·d^-1·m^-2 as compared to the individual films. Moreover, the ML film had the highest tensile strength at 0.56 MPa as compared to the mono-layer films including CH, SA, and EC with TS values of 0.33, 0.24 and 0.17 MPa, respectively. Furthermore, the AA-loaded ML film exhibited significantly higher DPPH scavenging activity at 66.20 %. These findings suggest that the ML film, due to its superior barrier, mechanical, and antioxidant properties has the potential for the applications in active food packaging.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"138169"},"PeriodicalIF":7.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A spatial hue smartphone-based colorimetric detection and discrimination of carmine and carminic acid in food products based on differential adsorptivity.","authors":"Aya A Abdella, Eman A Elshenawy","doi":"10.1016/j.talanta.2024.127053","DOIUrl":"10.1016/j.talanta.2024.127053","url":null,"abstract":"<p><p>A novel, portable, disposable, affordable, and environmentally friendly paper-based analytical device (PAD) was designed for on-site determination of carmine and carminic acid. This platform utilized paper test strips with a chitosan coating as an adsorption layer, which was characterized using scanning electron microscope, energy-dispersive X-ray analysis, and water contact angle measurement. Carmine and carminic acid could be efficiently adsorbed on chitosan-coated paper test strips, producing distinct colors that could be captured using a smartphone camera without the need for an elution step. Notably, by utilizing the Hue component of the HSL model, it was possible to differentiate between carmine and carminic acid, confirming their presence in a sample. Furthermore, the color saturation intensity changed in a concentration-dependent manner, allowing for the determination of carmine and carminic acid concentrations in the ranges of 200-800 μg/mL and 20-100 μg/mL, respectively. Additionally, the created test strip could be used to measure the percentage of carminic acid in the presence of carmine. The developed PAD enabled the quantification of carmine in various food samples without the need for reagents or complex equipment. The environmental impact of this method was found to be positive based on assessments using GAPI and AGREE tools.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"127053"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an untargeted DNA adductomics method by ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry.","authors":"Giorgia La Barbera, Marshal Spenser Shuler, Søren Hammershøj Beck, Per Holger Ibsen, Lars Joachim Lindberg, John Gásdal Karstensen, Lars Ove Dragsted","doi":"10.1016/j.talanta.2024.126985","DOIUrl":"10.1016/j.talanta.2024.126985","url":null,"abstract":"<p><p>Genotoxicants originating from inflammation, diet, and environment can covalently modify DNA, possibly initiating the process of carcinogenesis. DNA adducts have been known for long, but the old methods allowed to target only a few known DNA adducts at a time, not providing a global picture of the \"DNA adductome\". DNA adductomics is a new research field, aiming to screen for unknown DNA adducts by high resolution mass spectrometry (HRMS). However, DNA adductomics presents several analytical challenges such as the need for high sensitivity and for the development of effective screening approaches to identify novel DNA adducts. In this work, a sensitive untargeted DNA adductomics method was developed by using ultra-high performance liquid chromatography (UHPLC) coupled via an ESI source to a quadrupole-time of flight mass spectrometric instrumentation. Mobile phases with ammonium bicarbonate gave the best signal enhancement. The MS capillary voltage, cone voltage, and detector voltage had most effect on the response of the DNA adducts. A low adsorption vial was selected for reducing analyte loss. Hybrid surface-coated analytical columns were tested for reducing adsorption of the DNA adducts. The optimized method was applied to analyse DNA adducts in calf thymus, cat colon, and human colon DNA by performing a MS^E acquisition (all-ion fragmentation acquisition) and screening for the loss of deoxyribose and the nucleobase fragment ions. Fifty-four DNA adducts were tentatively identified, hereof 38 never reported before. This is the first untargeted DNA adductomics study on human colon tissue, and one of the few untargeted DNA adductomics studies in the literature reporting the identification of such a high number of unknowns. This demonstrates promising results for the application of this sensitive method in future human studies for investigating novel potential cancer-causing factors.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"282 ","pages":"126985"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A one-pot isothermal Fluorogenic Mango II arrays-based assay for label-free detection of miRNA.","authors":"Zan Gong, Panpan Yuan, Yuqing Gan, Xi Long, Zhiwei Deng, Yalan Tang, Yanjing Yang, Shian Zhong","doi":"10.1016/j.talanta.2024.126920","DOIUrl":"10.1016/j.talanta.2024.126920","url":null,"abstract":"<p><p>The capability to detect a small number of miRNAs in clinical samples with simplicity, selectivity, and sensitivity is immensely valuable, yet it remains a daunting task. Here, we described a novel Mango II aptamers-based sensor for the one-pot, sensitive and specific detection of miRNAs. Target miRNA-initiated mediated catalyzed hairpin assembly (CHA) would allow for the production of plenty of DNA duplexes and the formation of the complete T7 promoter, motivating the rolling circle transcription (RCT). Then, the subsequent RCT process efficiently generates a huge number of repeating RNA Mango II aptamers, brightened by the incorporation of fluorescent dye TO1-B for miRNA quantification, realizing label-free and high signal-to-background ratio. Moreover, this assay possesses a remarkable ability to confer high selectivity, enabling the distinction of miRNAs among family members with mere 1- or 2- nucleotide (nt) differences. By employing the proposed assay, we have successfully achieved a sensitive evaluation of miR-21 content in diverse cell lines and clinical serum samples. This offers a versatile approach for the sensitive assay of miRNA biomarkers in molecular diagnosis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126920"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of 4-n-butylresorcinol by fluorescence derivatization based on dopamine.","authors":"Qing Liu, Si Chen, Yiming Nie, Qian Li, Fang Chen","doi":"10.1016/j.talanta.2024.126909","DOIUrl":"10.1016/j.talanta.2024.126909","url":null,"abstract":"<p><p>4-n-butylresorcinol (4nBR) is a frequently utilized as whitening ingredients in skincare cosmetics. Compared with other whitening ingredients, it can effectively inhibit tyrosinase with lower toxicity and superior inhibition efficacy. Under alkaline conditions, an induced oxidative coupling reaction can occur between 4nBR and dopamine (DA) to generate strong fluorescent substance azamonardine with an intense emission band centering at 476 nm when excited at 440 nm. This phenomenon can be used to establish a fluorescence analysis method for 4nBR. The results indicated that the linear range of the method was 1.0-24.0 nmol L^-1, and the detection limit was as low as 0.25 nmol L^-1. The method showed high sensitivity, good selectivity, mild experimental conditions and low cost. The proposed method was successfully used to detect 4nBR in cosmetics, and the results were consistent with those of HPLC. The spiking recoveries were between 98.2% and 108 %.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126909"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palliative Care Consultation in the Intensive Care Unit Reduces Hospital Costs: A Cost-Analysis.","authors":"Tong Han Chung, Linh K Nguyen, Lincy S Lal, J Michael Swint, Yen-Chi L Le, Kathleen R Hanley, Efrain Siller, Cheryl M Chanaud","doi":"10.1177/08258597221095986","DOIUrl":"10.1177/08258597221095986","url":null,"abstract":"<p><strong>Background: </strong>Palliative care aims to improve or maintain quality of life for patients with life-limiting or life-threatening diseases. Limited research shows that palliative care is associated with reduced intensive care unit length of stay and use of high-cost resources.</p><p><strong>Methods: </strong>This was an observational, non-experimental comparison group study on all patients 18 years or older admitted to any intensive care unit (ICU) at Memorial Hermann - Texas Medical Center for 7 to 30 days from August 2013 to December 2015. Length of stay (LOS) and hospital costs were compared between the treatment group of patients with palliative care in the ICU and the control group of patients with usual care in the ICU. To adjust for confounding of the palliative care consultation on LOS and hospital cost, an inverse probability of treatment weighted method was conducted. Generalized linear models using gamma distribution and log link were estimated. All costs were converted to 2015 US dollars.</p><p><strong>Results: </strong>Mean LOS was 13 days and mean total hospital costs were USD 58,378. In adjusted and weighted analysis, LOS for the treatment group was 8% longer compared to the control group. The mean total hospital cost was estimated to decrease by 21% for the treatment group versus the control group. We found a reduction of USD 33,783 in hospital costs per patient who died in the hospital and reduction of USD 9113 per patient discharged alive.</p><p><strong>Conclusion: </strong>Palliative care consultation was associated with a reduction in the total cost of hospital care for patients with life-limiting or life-threatening diseases.</p>","PeriodicalId":51096,"journal":{"name":"Journal of Palliative Care","volume":"1 1","pages":"3-7"},"PeriodicalIF":16.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45777617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Practice Guidelines on Palliative Sedation Around the World: A Systematic Review.","authors":"Martyna Tomczyk, Cécile Jaques, Ralf J Jox","doi":"10.1177/08258597221138674","DOIUrl":"10.1177/08258597221138674","url":null,"abstract":"<p><p><b>Objective:</b> The principal aim of this study was to identify, systematically and transparently, clinical practice guidelines (CPGs) on palliative sedation from around the world. <b>Methods:</b> A systematic search was performed using 5 databases, grey literature search tools, citation tracking, and contact with palliative care experts across the world. Current CPGs accredited by an international, national, or regional authority, published in English, German, French, or Italian, were subjected to content analysis. <b>Results:</b> In total, 35 CPGs from 14 countries and 1 international CPG were included in the analysis. The CPGs had diverse formal characteristics. Their thematic scope was difficult to analyze and compare because of differences in the terms and definitions of palliative sedation in those texts. We identified 3 main situations: (1) CPGs <i>with</i> a fully explicit thematic scope; (2) CPGs <i>with</i> a partially explicit thematic scope; and (3) CPGs <i>without</i> an explicit thematic scope. Several CPGs explicitly stated what forms of sedation were excluded from the text. However, this presentation was often confusing. <b>Conclusion:</b> Our review provides several pieces of information that could guide international reflections in this field, and be used to develop or update CPGs at all levels. Efforts should be made to clarify the thematic scope of each CPG on palliative sedation, in order to generate an understanding of the forms of this therapy addressed in the text.</p>","PeriodicalId":51096,"journal":{"name":"Journal of Palliative Care","volume":" ","pages":"58-71"},"PeriodicalIF":16.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10332571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Practitioner Perspectives on Occupation-Based Practice Across Varied Settings.","authors":"Ben D Lee, Nancy Bagatell, Monica K Ridlehoover, Jennifer Womack","doi":"10.1177/15394492241226876","DOIUrl":"10.1177/15394492241226876","url":null,"abstract":"<p><p>Occupation-based practice (OBP) is considered a cornerstone of occupational therapy practice. However, there is no consensus-based definition of OBP, let alone agreement on its mediators across practice settings. To describe how OBP is currently conceptualized and enacted across different practice settings, a descriptive qualitative approach of focus groups and elicitation of visual data (e.g., photographs, video recordings) was used with 65 participants. Multiple rounds of qualitative data analysis were used to identify codes and themes. Three themes were identified: (a) contextual factors of OBP, (b) client status, and (c) temporal characteristics of OBP. OBP is influenced by institutional, historical, and temporal aspects of practice settings, with further exploration needed across different practice settings and occupational therapy pedagogy styles. Considering the influence of contextual aspects may support occupational therapy practitioners and researchers' efforts to support their advocacy for occupation as a foundational concept in the profession.</p>","PeriodicalId":47195,"journal":{"name":"Otjr-Occupation Participation and Health","volume":" ","pages":"140-147"},"PeriodicalIF":16.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139708197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valence fixable ferrozine gel rod combined with smartphone for facile determination of redox-active Fe²⁺ in environmental water.","authors":"Peng'an Zhu, Jiangle Zhang, Jingjing Jin, Xing Huang, Xinfeng Zhang","doi":"10.1016/j.talanta.2024.126933","DOIUrl":"10.1016/j.talanta.2024.126933","url":null,"abstract":"<p><p>Ferrous ion (Fe²⁺) can indicate the redox situation of water and also plays an important role in maintaining the ecological balance of water bodies. However, due to the redox-active property of Fe²⁺, it is still a huge challenge to sensitively and accurately determine Fe²⁺ especially in interstitial water. Herein, we prepared a ferrozine gel rod for valence fixation during sampling and subsequent smartphone-based detection of Fe²⁺. The electrode potential of the redox pair can be varied through the formation of Fe²⁺-ligand complexes, and when E_complex was higher than [Formula: see text] , the oxidation of Fe²⁺ by O₂ was hindered, thus achieving the valence fixation of Fe²⁺. Six ligands were screened, and it was found that ferrozine could effectively increase the redox potential after complexing with Fe²⁺, and also exhibits an obvious color change while fixing the valence of Fe²⁺. To facilitate Fe²⁺ detection, a cross-linked porous polymer gel rod prepared by acrylamide and sodium alginate was used to encapsulate the ferrozine molecules. The ferrozine gel rod enabled fixation the valence of Fe²⁺ longer than 30 days, and the resulted purple-red color was pictured and analyzed by a smartphone. Ultimately, the developed ferrozine gel rod sensing system was able to achieve sensitive and linear detection of Fe²⁺ in the range of 1-200 μM with the limit of detection as low as 0.33 μM, and it also exhibited excellent selectivity and anti-interference ability. The accuracy and reliability of the method was verified by the determination of Fe²⁺ in spiked water samples and certified standard reference water samples. Finally, the ferrozine gel rod sensing system was successfully applied to in-situ detection of Fe²⁺ in interstitial water, overlying water and upper water of lake and river. This facile system that enabled valence fixation and fast detection is promising for detection of Fe²⁺ in environmental waters.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"281 ","pages":"126933"},"PeriodicalIF":5.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}