IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-03-01 Epub Date: 2024-12-29 DOI: 10.1016/j.ijbiomac.2024.139327

Richard Ansah Herman, Xuan Zhu, Ellen Ayepa, Marriam Khurshid, Zhan-Peng Zhang, Shuai You, Jun-Feng Qian, Jun Wang

{"title":"Magnetic Janus SiO2 nanoparticles immobilized protease mutant T70I as a novel clarification agent for juice processing.","authors":"Richard Ansah Herman, Xuan Zhu, Ellen Ayepa, Marriam Khurshid, Zhan-Peng Zhang, Shuai You, Jun-Feng Qian, Jun Wang","doi":"10.1016/j.ijbiomac.2024.139327","DOIUrl":"10.1016/j.ijbiomac.2024.139327","url":null,"abstract":"The juice processing industry offers several benefits, including promoting health and wellness through the delivery of beverages rich in nutrients. Nonetheless, the industry encounters significant challenges regarding new technologies for processing and preservation given that they can be costly and labor-intensive. In this study, magnetic Janus silica (SiO2) nanoparticle which offers multifunctionality and high stability was synthesized and subsequently immobilized with a protease mutant T70I (T70I@MSNs) to serve as a clarifying agent in juice. The optimal conditions for the protease immobilized with SiO2 nanoparticles showed superior tolerance and stability at pH 5 and 65 °C. Box-Behnken Design revealed optimizing conditions of 60 min, 50 °C and 0.2 % enzyme concentration which significantly influenced the clarification of the juice samples. Immobilized T70I@MSNs decreased >70 % of juice turbidity while maintaining about 78 % of enzyme activity after 7 consecutive cycles of reuse. Notably, T70I@MSN treatment enhanced certain volatile components by increasing alcohol and ester production, while changes in surface microstructure and functional groups were observed. This study highlights the significant improvement of magnetic Janus SiO2 nanoparticles immobilized proteases in the clarification of juice against haze and undesirable properties, thereby offering a notable contribution to the juice processing industry.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139327"},"PeriodicalIF":7.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parenting Attitudes and Abusive Parenting among Incarcerated Mothers in Japan. 日本被监禁母亲的养育态度和虐待性养育。

IF 16.4 1区化学

Accounts of Chemical Research Pub Date : 2025-03-01 Epub Date: 2022-10-12 DOI: 10.1177/0306624X221124853

Ayako Sasaki, Akemi Mochizuki, Daiki Yoshihara

引用次数: 0

Bioreactor contamination monitoring using off-gassed volatile organic compounds (VOCs). 利用废气挥发性有机化合物（VOCs）监测生物反应器污染。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-03-01 Epub Date: 2024-12-26 DOI: 10.1007/s00216-024-05720-z

Angela L Linderholm, Manohar P Bhandari, Eva Borras, Allison Kwon, Flore M Herve, Mitchell M McCartney, Richart W Harper, Nicholas J Kenyon, Cristina E Davis

{"title":"Bioreactor contamination monitoring using off-gassed volatile organic compounds (VOCs).","authors":"Angela L Linderholm, Manohar P Bhandari, Eva Borras, Allison Kwon, Flore M Herve, Mitchell M McCartney, Richart W Harper, Nicholas J Kenyon, Cristina E Davis","doi":"10.1007/s00216-024-05720-z","DOIUrl":"10.1007/s00216-024-05720-z","url":null,"abstract":"Metabolically active cells emit volatile organic compounds (VOCs) that can be used in real time to non-invasively monitor the health of cell cultures. We utilized these naturally occurring VOCs in an adapted culture method to detect differences in culturing Chinese hamster ovary (CHO) cells with and without Staphylococcus epidermidis and Aspergillus fumigatus contaminations. The VOC emissions from the cell cultures were extracted and measured from the culture flask headspace using polydimethylsiloxane (PDMS)-coated Twisters, which were subjected to thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) analysis. In our initial time points of 1 and 2 h, we detected VOC signatures that differentiated the cultures earlier than traditional plating techniques or visualization methods. Partial least squares-discriminant analysis (PLS-DA) models were built to differentiate the analytes from the CHO cells and S. epidermidis- and A. fumigatus-inoculated CHO cultures. A total of 41 compounds with a variable importance in projection (VIP) score greater than 1 was obtained across the models. Similarly, based on the PLS regression analyses to predict the cell concentration of S. epidermidis (R2 = 0.891) and A. fumigatus (R2 = 0.375), 15 and 20 relevant compounds were putatively identified, respectively; two known compounds overlapped between the two microbes. Some of the compounds were unidentified and future studies will determine the relationship between the VOCs and the metabolic changes in contaminated cultures.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1165-1176"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of high hydraulic pressure on the short-term retrogradation and digestive properties of Lonicern caerulea berry polyphenol-chestnut starch complexes. 高水压对金银花浆果多酚-栗子淀粉复合物短期降解及消化性能的影响。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-03-01 Epub Date: 2024-12-29 DOI: 10.1016/j.ijbiomac.2024.139242

Suwen Liu, Zhang Lu, Kai Zhang, Runzheng Wang, Xuedong Chang, Jingzheng Zhang

{"title":"Effects of high hydraulic pressure on the short-term retrogradation and digestive properties of Lonicern caerulea berry polyphenol-chestnut starch complexes.","authors":"Suwen Liu, Zhang Lu, Kai Zhang, Runzheng Wang, Xuedong Chang, Jingzheng Zhang","doi":"10.1016/j.ijbiomac.2024.139242","DOIUrl":"10.1016/j.ijbiomac.2024.139242","url":null,"abstract":"Both fresh and processed Chinese chestnuts are susceptible to retrograde hardening, affecting their texture, flavor, and shelf life because of their high starch content. To reduce the short-term retrogradation of chestnut starch during the food processing of chestnut-based products, a complex of Lonicern caerulea berry polyphenols (LCBP) and chestnut starch (CS) was prepared using high hydraulic pressure (HHP). The results showed that LCBP reduced the water separation rate, hardness, elasticity, and short-range order of retrograde CS under HHP and improved light transmission. After aging for 1 day, the relative crystallinity of 600 MPa-8 % LCBP-CS was significantly reduced by 53.1 % compared with CS (p < 0.05), and its particle size distribution was more uniform, with a complexation rate of 63.9 %. Under the same pressure, the complex with 8 % LCBP showed a more significant short-term retrograde inhibitory effect. In addition, the resistant starch content of 600 MPa-8 % LCBP-CS was 61 %. Correlation analysis showed that the complexation rates of LCBP and CS positively correlated with short-term retrogradation and digestive resistance. In summary, HHP facilitates the formation of a LCBP-CS complex that inhibits short-term retrogradation and enhances digestive resistance, aiding in the development of hypoglycemic chestnut products with extended shelf lives.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139242"},"PeriodicalIF":7.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation, characterization, and antibacterial and antioxidant activities of caffeic acid grafted ε-polylysine. 咖啡酸接枝ε-聚赖氨酸的制备、表征及抗菌抗氧化活性研究。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-03-01 Epub Date: 2024-12-30 DOI: 10.1016/j.ijbiomac.2024.139276

Junhui Zhu, Zhiying Lei, Yali Tang, Lixin Lu, Xiaolin Qiu, Liao Pan

{"title":"Preparation, characterization, and antibacterial and antioxidant activities of caffeic acid grafted ε-polylysine.","authors":"Junhui Zhu, Zhiying Lei, Yali Tang, Lixin Lu, Xiaolin Qiu, Liao Pan","doi":"10.1016/j.ijbiomac.2024.139276","DOIUrl":"10.1016/j.ijbiomac.2024.139276","url":null,"abstract":"The antioxidant activity of ε-polylysine (EPL) can be enhanced by grafting phenolic compound caffeic acid (CA) onto its amino groups. To enhance the antioxidant activity of EPL, this study synthesized caffeic acid-ε-polylysine conjugate (CA-EPL) by grafting CA onto EPL using carbodiimide coupling reaction. Fourier transform infrared spectroscopy, 1H nuclear magnetic resonance (NMR) spectroscopy confirmed the successful conjugation of caffeic acid and ε-polylysine. The ultraviolet-visible absorption spectra, grafting ratio, and Zeta potential data indicate that the molar ratio of CA to EPL has a significant impact on the grafting degree and Zeta potential of the conjugates. In particular, the highest grafting degree and the lowest Zeta potential were obtained when the molar ratio of carboxyl groups in CA to amino groups in EPL was 3:1. Furthermore, the antimicrobial and antioxidant activities of the conjugates were evaluated. The results of antimicrobial activity indicate that the conjugate CA-EPL still exhibits excellent antimicrobial properties. The results of antioxidant activity show a significant increase in the antioxidant activity of the conjugate CA-EPL, which was significantly higher than that of free EPL. In addition, the research results on the antimicrobial mechanism show that CA-EPL has a similar antimicrobial mechanism to EPL: by interacting with the bacterial cell membrane, it disrupts the cell membrane, causing leakage of cell contents, ultimately leading to bacterial death. These results indicate that CA-EPL, as a novel dual-functional active substance, has broad prospects in the food and pharmaceutical industries.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139276"},"PeriodicalIF":7.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of L-Met functionalized cellulose nanofiber for an efficient removal of Pb²⁺ from aqueous solution: Characterization, adsorption behavior, DFT calculations and physiochemical mechanism. L-Met功能化纤维素纳米纤维对Pb2+的高效去除：表征、吸附行为、DFT计算和理化机理

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-03-01 Epub Date: 2024-12-30 DOI: 10.1016/j.ijbiomac.2024.139180

Simiao Wu, Haifan Yang, Guannan Liang, Xinyang Sun

{"title":"Development of L-Met functionalized cellulose nanofiber for an efficient removal of Pb2+ from aqueous solution: Characterization, adsorption behavior, DFT calculations and physiochemical mechanism.","authors":"Simiao Wu, Haifan Yang, Guannan Liang, Xinyang Sun","doi":"10.1016/j.ijbiomac.2024.139180","DOIUrl":"10.1016/j.ijbiomac.2024.139180","url":null,"abstract":"Lead-containing wastewater has been a significant challenge in the field of wastewater treatment. Cellulose surface has a large number of active sites, which is conducive to load modification. And amino acids have rich functional groups, which is a good choice for cellulose modification. In this study, the non-toxic L-methionine modified cellulose nanofiber (M-CNF) adsorbent was developed to adsorb Pb2+ in aqueous solution. M-CNF exhibited a maximum adsorption efficiency of 98.24 ± 0.04 %. The theoretical adsorption capacity could reach 113.3 ± 13.4 mg g-1. After five cycles of adsorption-desorption process, the removal efficiency of Pb2+ was still >80 %. The pseudo-second-order kinetic model (R2 = 0.9913) and Brunauer-Emmet-Teller adsorption isotherm model (R2 = 0.9916) can be used to describe the adsorption behavior of M-CNF on Pb2+. The Gibbs free energy (ΔG) and enthalpy (ΔH) of the reaction are negative, indicating that the reaction was a spontaneous exothermic process. Density functional theory calculation indicated that -COOH and -C-S-C- groups played a role in the adsorption process. The adsorption mechanism of M-CNF might be the interaction of ion exchange, electrostatic adsorption, complexation and van der Waals force. This work suggested that M-CNF played a definitive role in the adsorption and removal of Pb2+ in aqueous solutions.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139180"},"PeriodicalIF":7.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A molecularly imprinted ratio fluorescence sensor based on metal-enhanced fluorescence of core-shell structure CaF₂-silver nanoparticle for visual detection of dicamba. 基于核壳结构caf2 -银纳米颗粒金属增强荧光的分子印迹比例荧光传感器用于麦草畏的视觉检测。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-03-01 Epub Date: 2025-01-09 DOI: 10.1007/s00216-024-05715-w

Jing Xu, Jiechun Wu, Songlian Li, Zhihong Yan, Qing Zhou, Kang Li

{"title":"A molecularly imprinted ratio fluorescence sensor based on metal-enhanced fluorescence of core-shell structure CaF2-silver nanoparticle for visual detection of dicamba.","authors":"Jing Xu, Jiechun Wu, Songlian Li, Zhihong Yan, Qing Zhou, Kang Li","doi":"10.1007/s00216-024-05715-w","DOIUrl":"10.1007/s00216-024-05715-w","url":null,"abstract":"Although fluorescence analysis methods are widely used in pesticide residue detection, improving their sensitivity and selectivity remains a challenge. This paper presents a novel ratio fluorescence sensor based on the molecular imprinting polymers (MIPs) and metal-enhanced fluorescence for visual detection of dicamba (DIC). Calcium fluoride (CaF2) quantum dots (QDs) were immobilized on the surface of Ag@MIPs, resulting in a blue fluorescence response signal (Ag@MIPs-CaF2). The MIPs shell, which possesses a specific recognition capability, serves as an isolation layer to adjust the distance between Ag nanoparticles and CaF2 QDs for enhancing the fluorescence of CaF2 QDs by up to 7.1 times under optimal conditions. In the presence of DIC, the blue fluorescence was selectively quenched, while the reference signal red fluorescence from cadmium telluride QDs coated with silicon dioxide (CdTe@SiO2) remained relatively stable, resulting in a color change from blue to red. The sensor had a detection limit of 0.16 μM for DIC in the range of 1.0 to 50.0 μM, recovery rates of 85.4 to 103.5% for actual samples, and an imprinting factor of 3.28. The 3D finite-difference time-domain simulation revealed that the fluorescence enhancement was due to the local electric field amplification. Therefore, the developed sensing system in this work offers a broad application prospect for visual detection of DIC in food samples.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1139-1152"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient adsorption and detection of steroid hormones in foods through the combination of novel magnetic TAPB-COF materials with click isotope probes. 新型磁性TAPB-COF材料与click同位素探针结合对食品中类固醇激素的高效吸附和检测。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-03-01 Epub Date: 2025-01-17 DOI: 10.1007/s00216-024-05727-6

Jiaqi Xu, Qianyu Li, Wenrui Li, Di Wu, Yongning Wu, Guoliang Li

{"title":"Efficient adsorption and detection of steroid hormones in foods through the combination of novel magnetic TAPB-COF materials with click isotope probes.","authors":"Jiaqi Xu, Qianyu Li, Wenrui Li, Di Wu, Yongning Wu, Guoliang Li","doi":"10.1007/s00216-024-05727-6","DOIUrl":"10.1007/s00216-024-05727-6","url":null,"abstract":"Matrix effects pose a significant challenge in food analysis for the quantitative analysis of complex food samples. Herein, a novel magnetic covalent organic framework nanocomposite and the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction-based stable isotope labeling (SIL) method were presented for highly selective and sensitive detection of steroid hormones in food samples using HPLC-MS/MS. The nanocomposite, Fe3O4@TAPB-COF, with a core-shell structure exhibited high adsorption capacities for steroid hormones. Combined with a SIL method based on the CuAAC click reaction, steroid hormones were accurately quantified in food samples with high sensitivity and selectivity. A pair of SIL agents, N-(2-azidoethyl)aniline (d0-NAEA) and d5-N-(2-azidoethyl)aniline (d5-NAEA), was synthesized to label steroid hormones in the samples and standard solutions, respectively. The labeling reaction is highly specific, and the formation of the derivatives is easily ionized by MS, thus overcoming matrix effects. More surprisingly, the ionization efficiency of steroid hormones increased by a factor of 4 to 56, with matrix effects ranging from 87.3 to 99.3%. Under optimal conditions, this method exhibited a low limit of detection (LOD) ranging from 0.1 to 2.6 μg L-1 and overcame the interference of matrix effects for trace-level steroid hormone analysis in foodstuffs.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1199-1212"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of bigel systems as carriers for thyme essential oil utilizing hydrogel from chicken processing by-products for food applications. 利用鸡肉加工副产品水凝胶作为百里香精油载体的bigel体系的开发和表征。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-03-01 Epub Date: 2024-12-26 DOI: 10.1016/j.ijbiomac.2024.139222

William Oyom, Jordyn Strange, Kyle Nowlin, Panesun Tukur, Md Jannatul Ferdaus, Habibollah Faraji, Reza Tahergorabi

{"title":"Development and characterization of bigel systems as carriers for thyme essential oil utilizing hydrogel from chicken processing by-products for food applications.","authors":"William Oyom, Jordyn Strange, Kyle Nowlin, Panesun Tukur, Md Jannatul Ferdaus, Habibollah Faraji, Reza Tahergorabi","doi":"10.1016/j.ijbiomac.2024.139222","DOIUrl":"10.1016/j.ijbiomac.2024.139222","url":null,"abstract":"A chicken protein hydrogel (HG) was enzymatically prepared and blended with a carnauba wax-based oleogel (OG) to form bigels (BG) in ratios of 50:50 to 90:10. These systems were infused with thyme essential oil (TEO) at 0.5 %, 1 %, and 2 % v/v to harness its antioxidant properties. Polarized light microscopy revealed that carnauba wax crystals were tightly arranged in thin, plate-like structures, while the HG exhibited a completely amorphous form. FT-IR analysis indicated that OH bonds in the HG and CH bonds in cycloalkanes from the OG contributed to the stability and strength of the gels. Unlike the biphasic gels, both the OG and HG samples experienced structural disintegration when the applied strain reached approximately 30 %. HG samples, with an onset melting temperature of 59.18 °C, were particularly susceptible to thermal deformation, leading to coalescence and destabilization of the BG due to the HG phase in the composite matrix. Regardless of the TEO concentrations, the BG (50:50) demonstrated the highest water-holding capacity (60.83 %), and oil-binding capacity (99.23 %) compared to the single biopolymer gels. The lightness of the BG increased as the HG ratio increased. Additionally, the antioxidant capacity increased with higher TEO concentrations, demonstrating the gels' potential for food applications.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139222"},"PeriodicalIF":7.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PARylation facilitates the DNA damage repair of Phytophthora sojae in response to host ROS stress. PARylation促进大豆疫霉对宿主ROS胁迫的DNA损伤修复。

IF 7.7 1区化学

International Journal of Biological Macromolecules Pub Date : 2025-03-01 Epub Date: 2024-12-28 DOI: 10.1016/j.ijbiomac.2024.139069

Fan Zhang, Shanshan Chen, Can Zhang, Zhiwen Wang, Jianqiang Miao, Tan Dai, Jianjun Hao, Xili Liu

{"title":"PARylation facilitates the DNA damage repair of Phytophthora sojae in response to host ROS stress.","authors":"Fan Zhang, Shanshan Chen, Can Zhang, Zhiwen Wang, Jianqiang Miao, Tan Dai, Jianjun Hao, Xili Liu","doi":"10.1016/j.ijbiomac.2024.139069","DOIUrl":"10.1016/j.ijbiomac.2024.139069","url":null,"abstract":"Host plants and various fungicides combat plant pathogens by triggering the release of excessive ROS, leading to DNA damage and subsequent cell death. The mechanisms by which the Phytophthora sojae mitigates ROS stress induced by plant immune responses and fungicides are not well understood. This study investigates the role of PsPARP1A-mediated poly (ADP-ribosylation) (PARylation) in ROS-induced DNA damage responses (DDR). Mechanistically, Phytophthora sojae poly (ADP-ribose) polymerase (PsPARP1A) interacts with meiotic recombination 11 (PsMRE11) to facilitate the accumulation of histone H2Ax phosphorylated on serine 137 (γH2Ax) in response to plant ROS-induced DNA damage. The PARylation of PsMRE11 by PsPARP1A at E5, D7, D8, and E12 is critical for the nuclear localization of PsMRE11 and the subsequent accumulation of γH2Ax during DNA damage induced by host defense-generated ROS stress in P. sojae. These findings underscore the pivotal role of the PsPARP1A-PsMRE11 axis in DNA damage repair and adaptation to ROS, thereby contributing to the virulence of P. sojae. Our study highlights the novel functions of PsPARP1/PsMRE11 in pathogenic oomycetes, linking PARylation-dependent DDR processes to their development and virulence.","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"139069"},"PeriodicalIF":7.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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