Chineae Journal of Organ Transplantation最新文献

{"title":"Analysis of influencing factors of blood concentration of tacrolimus in Chinese pediatric living donor liver transplant patients","authors":"Yi Qin, Xiaoshuo Wang, Meiling Yan, Wei Gao, Fan Chen, Yi Zhang","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.02.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.02.009","url":null,"abstract":"Objective \u0000To explore the influencing factors of blood concentration of tacrolimus in pediatric living donor liver transplant recipients and provide rationales for individualized administration of tacrolimus. \u0000 \u0000 \u0000Methods \u0000Trough concentrations (C0), doses of tacrolimus, recipient age, gender, body weight, donor and recipient CYP3A5 genotypes, hematocrit (HCT) and liver/kidney function related indicators at 3, 5, 7, 14 days, 1 month, 2 months and 3 months post living donor liver transplantation were collected from a total of 100 pediatric recipients. Taking ratio of concentration to dose (C0/D) as a dependent variable, the influencing factors of blood concentration of tacrolimus were analyzed by multivariate stepwise regression. \u0000 \u0000 \u0000Results \u0000The influencing factors of blood tacrolimus concentration at 3d post-transplantation were recipient CYP3A5 genotyp, donor CYP3A5 genotype and weight of recipients. The major influencing factors at 5d post-transplantation were recipient & donor CYP3A5 genotypes, recipient weight and HCT. The major relevant factors at 7d post-transplantation were CYP3A5 of recipients, age and HCT. The influencing factors at 14 days were the same as those at 2 months, i. e. CYP3A5 genotype and weight of recipients. At 1 month the major influencing factors were weight of recipients, CYP3A5 of recipients and alkaline phosphatase (ALP); CYP3A5 genotype and weight of recipients at 3 months. Further study on CYP3A5 genotype of donors and recipients, the C0/D ratio of CYP3A5 genotype non-expression group was significantly higher than that of expression group in recipients and C0/D ratio of donor CYP3A5 genotype non-expression group was significantly higher than that of expression group. \u0000 \u0000 \u0000Conclusions \u0000The influencing factors of concentration of tacrolimusvary at different timepoints after liver transplantation. Paying close attention to the changes of CYP3A5 genotype, weight of recipients and related biochemical indexes and considering various influencing factors facilitate individualized dosing for improving the prognosis of pediatric recipients. \u0000 \u0000 \u0000Key words: \u0000Pediatric; Liver transplantation; Living donor; Tacrolimus; Blood concentration","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78188411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of ETaR siRNA on renal ischemia-reperfusion injury in rats by changing the immuno-microenvironment of kidney","authors":"Yichen Jia, Long Zheng, Long Li, Jiawei Li, Ming Xu, T. Zhu","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.02.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.02.002","url":null,"abstract":"Objective \u0000To explore the protective effect of ETaR siRNA on renal ischemia reperfusion injury (IRI) by changing the immuno-microenvironment in rats. \u0000 \u0000 \u0000Methods \u0000A total of 40 male Sprague-Dawley (SD) rats were randomized into four groups of sham, IR, negative siRNA and ETaR siRNA. A renal IRI model was generated by clamping left renal artery. ETaR siRNA was delivered into kidney through renal vein by a retrograde 'hydrodynamic’ injection. Blood samples were collected for detecting renal function and kidney tissue harvested for Hematoxylin & Eosin (HE) staining, TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, polymerase chain reaction (PCR) and Western blot at 48 h post-reperfusion. \u0000 \u0000 \u0000Results \u0000Serum creatinine, blood urea nitrogen and renal apoptotic cells increased and renal tissue was injured after IR. The changes were inhibited by ETaR siRNA. PCR showed that ETaR siRNA treatment significantly down-regulated the expressions of inflammatory factors TNF-α, IFN-γ and IL-6 and transcription factor NF-κB induced by IR. \u0000 \u0000 \u0000Conclusions \u0000ETaR siRNA can effectively improve the immuno-microenvironment and thereby alleviate renal ischemia reperfusion injury. \u0000 \u0000 \u0000Key words: \u0000Rats; Kidney; Endothelin receptor; Small interfering RNA; Immuno-microenvironment; Reperfusion injury","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75065342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of islet allograft rejection by Qa-1/PD-L1 artificial liposome","authors":"M. Guo, Zhao Yuanyu, Hao Yin, Jia-Yong Dong, Ji Junsong, Lu‐Yue Qi, Hang Yuan, F. Teng, Wen-Yuan Guo","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.02.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.02.003","url":null,"abstract":"Objective \u0000To explore the effects of Qa-1 and PD-L1 loaded artificial liposomal treatment in allograft rejection and its outcomes. \u0000 \u0000 \u0000Methods \u0000The extracellular domains of Qa-1 and PD-L1 were loaded on liposome surface by streptavidin-biotin system. Mixed lymphocyte reaction (MLR) was performed for measuring Qa-1/PD-L1 liposome biological function. Then liposome was co-transplanted with allo-islets via portal vein. The levels of blood glucose and C-peptide were detected daily after transplantation. Also hepatic lymphocytes after transplantation were isolated for determining the proportion of activated cells and signaling pathway changes. \u0000 \u0000 \u0000Results \u0000Artificial liposome could be easily loaded with biotinylated peptide and its diameter was between 50 to 500 nm. Qa-1/PD-L1 liposome could significantly suppress lymphocyte proliferation, activation and secretion of IFN-γ in MLR by an activation of SHP1/2 and an inhibition of Syk pathway. Qa-1/PD-L1 liposomes could suppress the activation of hepatic lymphocytes in vivo by activating SHP1/2, protecting islet allografts and maintaining a normal level of blood glucose in recipients. \u0000 \u0000 \u0000Conclusions \u0000Qa-1/PD-L1 loaded liposome can effectively suppress allograft rejection and improve the outcomes of islet transplantation. \u0000 \u0000 \u0000Key words: \u0000Mouse; Islet transplantation; Qa-1; PD-L1; Graft rejection; SHP1/2","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77559206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of unrelatedversus matched-sibling donor allogeneic hematopoietic stem cell transplantation for leukemic children","authors":"Binglei Zhang, Jian Zhou, Yanli Zhang, R. Gui, Yuewen Fu, Y. Zu, F. Yu, Hui-fang Zhao, Zhen Li, B. Fang, Xudong Wei","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.02.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.02.012","url":null,"abstract":"Objective \u0000To evaluate the efficacy of unrelated donor allogeneic hematopoietic stem cell transplantation (allo-HSCT) for leukemic children. \u0000 \u0000 \u0000Methods \u0000Clinical data of 54 leukemic children undergoing allo-HSCT were retrospectively analyzed from May 2006 to March 2018. According to the source of donor, they were divided into matched sibling donor allo-HSCT group (MSD, n=27) and unrelated donor group (URD, n=27). The clinical outcomes of leukemic children receiving URD allo-HSCT were assessed and those in MSD allo-HSCT group were enrolled as control. \u0000 \u0000 \u0000Results \u0000One patient with refractory AML was not implanted in URD group and the remaining 53 cases were successful in hematopoietic reconstitution. The time of neutrophil and platelet, the incidence of acute graft-versus-host disease (aGVHD), chronic GVHD (cGVHD), generalized cGVHD and their transplant-related complications including pulmonary complications, hemorrhagic cystitis between two groups were not statistically different (P>0.05). The incidence of serious aGVHD, cytomegalovirus (CMV) and EB virus (EBV) infection was significantly higher in URD group than that in MSD group (P 0.05). \u0000 \u0000 \u0000Conclusions \u0000In leukemic children, although the incidence of complications post URD allo-HSCT is significantly increased, the prognosis is comparable to MSD allo-HSCT. It is a good choice when there is no suitable sibling donor. \u0000 \u0000 \u0000Key words: \u0000Allogeneic hematopoietic stem cell transplantation; Children; Leukemia; Unrelated donor; Matched sibling donor","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74631264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine prevents steatotic liver ischemia reperfusion injury by inhibiting endoplasmic reticulum stress and autophagy","authors":"Nan Zhang, M. Sheng, Man Wu, Xinyue Zhang, Yijie Ding, Wenli Yu, H. Du","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.02.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.02.013","url":null,"abstract":"Objective \u0000To explore the effect of berberine (BBR) on steatotic liver ischemia reperfusion injury and analyze the role of endoplasmic reticulum stress and autophagy. \u0000 \u0000 \u0000Methods \u0000Thirty-four Wistar rats were fed with a high-fat diet for 12 weeks and 2 rats were randomly selected after 8 weeks to observe pathological changes and confirm the model of steatotic liver successfully. Then before opening and closing abdominal cavity, 32 rats were divided into I/R group (normal saline was intragastrically 4 weeks before performing cold I/R treatment), BBR group (normal saline was replaced by BBR, BBR was intragastrically at a dose of 300 mg·kg-1·d-1 weeks and others were the same as I/R group) and TG group (TG was intraperitoneally at a dose of 0.2 mg·kg-1 24h pre-operation and others were the same as BBR group ). Then the rats were sacrificed at 6h post-reperfusion. Blood samples were collected from inferior vena cava and hepatic tissues harvested. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected, histopathologic changes observed by Hematoxylin & Eosin (HE) staining, oxidative stress and inflammation determined by ELISA kit and the expressions of p-PERK, CHOP, Bip, LC3, Beclin-1 and p62 detected by Western blot. \u0000 \u0000 \u0000Results \u0000As compared with Sham group, the serum levels of ALT and AST were significantly higher in I/R, BBR and TG groups (P<0.05). And hepatic histological changes were severe and oxidative stress increased in parallel with the enhancement of pro-inflammation (P<0.05). In BBR group, the level of hepatic enzymes declined, liver injury was milder, oxidative stress decreased and pro-inflammation was lesser compared with I/R and TG groups (P<0.05). Additionally, as compared with sham group, the expressions of p-PERK, CHOP, Bip, LC3, Beclin-1 and p62 were up-regulated in I/R and BBR groups (P<0.05). TG group increased the levels of LC3, Beclin-1 and p62 (P<0.05). Interestingly, compared with I/R group, BBR pretreatment down-regulated the expressions of p-PERK, CHOP, Bip, LC3, Beclin-1 and p62 (P<0.05). TG group had the higher expressions of LC3, Beclin-1 and p62 than those of BBR group (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000BBR pretreatment can protect steatotic liver ischemia reperfusion injury. And the mechanisms may be attributed to the inhibitions of endoplasmic reticulum stress and autophagy. \u0000 \u0000 \u0000Key words: \u0000Rat; Steatotic liver; Berberine; Ischemia reperfusion injury; Endoplasmic reticulum stress; Autophagy","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88210528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of image post-processing technique to measure spleen volume and evaluate the effect of orthotopic liver transplantation on relieving hypersplenism","authors":"Jian He, Qingjun Guo, Yan Xie, Li Zhang, D. Tian, Hong-hai Wang, Chiyi Chen, Wentao Jiang","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.02.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.02.010","url":null,"abstract":"Objective \u0000To employ image post-processing technique measuring splenic volume for evaluating the mitigation effect of end-stage liver disease patients complicated with different degrees of hypersplenism undergoing orthotopic liver transplantation. \u0000 \u0000 \u0000Methods \u0000For 55 end-stage liver disease patients with hypersplenism undergoing orthotopic liver transplantation, the changes in splenic volume were measured before and after transplantation by image post-processing system Advantage Workstation 46 (AW46) and the changes of splenic thickness, portal flow velocity and platelet counts observed during perioperative period. \u0000 \u0000 \u0000Results \u0000Postoperative splenic volumes of 55 recipients were (562.90±49.16) cm3, significantly decreased than preoperative (850.50±77.99) cm3 (P<0.05) and reduction ratio was (31.70±2.76)%. Splenic thickness at different postoperative timepoints was significantly lower than that pre-operation (P<0.05) and stabilized at 1 month post-transplantation; Splenic volume was positively correlated with splenic thickness (r=0.78, P<0.05). Portal flow velocity at different postoperative timepoints increased significantly as compared with preoperative (P<0.05), peaked at (380.70±21.80) mm/s at 1 month post-transplantation, declined and stabilized at 3 months post-transplantation. Platelet counts (PLT) at different postoperative timepoints were significantly higher than those at pre-operation (P<0.05), peaked (193.40±10.36)×109/L at 2 weeks post-transplantation, dropped and remained at 2 months post-transplantation; Splenic volume was negatively correlated with PLT (r=-0.44, P<0.05). And hypersplenism recovery rate and recurrence rate within 10 months post-transplantation was (78.79±2.29)% and (17.75±2.31)% respectively. \u0000 \u0000 \u0000Conclusions \u0000Orthotopic liver transplantation can effectively alleviate hypersplenism for most end-stage liver diseases. Using image post-processing system, splenic volume may be calculated and blood routine and ultrasound are simultaneously used for assessing the outcomes of liver transplantation on hypersplenism. \u0000 \u0000 \u0000Key words: \u0000Hypersplenism; Liver transplantation; Spleen volume; Platelet","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91328210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABO-incompatible kidney transplantation in highly presensitized recipients using deceased donors: a case report and literature review","authors":"Lan Zhu, H. Feng, Lu Wang, Zhiliang Guo, Juan Wang, Liang Huang, Hui Guo, Gang Chen","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.02.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.02.005","url":null,"abstract":"Objective \u0000To explore the feasibility and safety of kidney transplantation in highly sensitized recipients by using ABO incompatible (ABOi) and yet human leucocyte antigen (HLA) supremely matched deceased donor kidneys and summarize the literatures as well. \u0000 \u0000 \u0000Methods \u0000A kidney graft from a deceased donor of blood type B was transplanted to a highly presensitized recipient of blood type O to achieve a HLA matching number of 7/8 in May 2018. Donor specific antibody (DSA) against HLA was negative and baseline anti-B IgM 1∶16. Plasmapheresis (PP) plus intravenous immunoglobulin (IVIG) plus anti-CD20 antibodies were offered on operation day. Clinical data was retrospectively analyzed. \u0000 \u0000 \u0000Results \u0000Renal graft functioned immediately and achieved a normal level of serum creatinine (SCr) at d2 after transplantation. However, the value of SCr increased to 131 μmol/l at d9 with a simultaneously elevated level of anti-B IgM from 1∶2 at d7 to 1∶16. A renal graft biopsy at d11 showed mild inflammation in peritubular capillaries and focal tubulitis with minimal interstitial infiltration. No de novo DSA was detected. Then PP plus IVIG were then given twice, followed by an administration of IVIG alone for another 2 days (20 g/d). After treatments, SCr had a range of 120-140 μmol/l and anti-B IgM level decreased to 1∶4 at d21 post-transplantation. During a follow-up of 6 months, there was no onset of proteinuria or infection and the last value of SCr was 114 μmol/L. \u0000 \u0000 \u0000Conclusions \u0000In HLA highly sensitized recipients awaiting for transplant opportunities, successful prevention of HLA antibodies-mediated rejection may be achieved by using ABO incompatible and yet HLA compatible deceased donors. \u0000 \u0000 \u0000Key words: \u0000Kidney transplantation; Sensitization; ABO incompatible; HLA; Donor specific antibodies","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77990119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical analysis of persistent hypercalcemia with osteoporosis caused by tertiary hyperparathyroidism after kidney transplantation","authors":"Su-ya Wang, Jianyong Wu, W. Peng, Rending Wang, Hongfeng Huang","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.02.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.02.007","url":null,"abstract":"Objective \u0000To explore the clinical characteristics of tertiary hyperparathyroidism (THPT) after renal transplantation. \u0000 \u0000 \u0000Methods \u0000The levels of bone mineral density (BMD), serum calcium, phosphates, alkaline phosphatase (ALP) and intact parathyroid hormone (iPTH) were retrospectively analyzed in 36 RTx recipients with persistent hypercalcemia and stable kidney function (eGFR 76.71±17.44) ml/min/1.73 m2. \u0000 \u0000 \u0000Results \u0000Among them, serum total calcium level increased (2.97±0.20) mmol/L for 6 to 170 months, blood phosphorus decreased (0.59±0.19) mmol/L, serum alkaline phosphatase (ALP) increased to (295.73±194.22)U/L and T-score of BMD decreased (T-2.78±0.84 in lumbar vertebrae and T-2.09±0.66 in hip joint). And 11/36 (30.6%) cases had a complication of extraosseous calcification. Parathyroid hyperplasia was detected in 17/36 cases (47.2%). iPTH was significantly higher at pre-operation and 1 week post-operation than that in control group (n=45) (859.50±495.44 vs 345.56±216.55 pg/ml), P=0.001, (759.25±907.07 vs 197.45±249.31 pg/ml), P=0.001. The value of iPTH at the last follow-up (198.26±155.22) pg/ml was still higher than normal reference value (15.0-65.0 pg/ml). Multivariate stepwise regression analysis showed the last iPTH was correlated with preoperative iPTH, serum calcium and postoperative serum phosphor, ALP and 25OHD3 (P=0.024, P=0.002, P=0.001, P=0.037, P=0.026). \u0000 \u0000 \u0000Conclusions \u0000Renal recipients had a higher levels iPTH with persistent hypercalcemia, hypophosphatemia, osteoporosis and extraosseous calcification showing the features of tertiary hyperparathyroidism. \u0000 \u0000 \u0000Key words: \u0000Kidney transplantation; Tertiary hyperparathyroidism; Hypercalcemia; Osteoporosis","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88026952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different induction therapies on the clinical outcomes of ABO-incompatible living donor kidney transplantation recipients","authors":"W. Lei, Shuaihui Liu, Jing-yi Zhou, Jia Shen, W. Xie, X. Yao, Menger Cen, Jianghua Chen","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.02.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.02.004","url":null,"abstract":"Objective \u0000To compare the clinical outcomes of low-dose rabbit antithymocyte globulin (rATG) vs basiliximab as induction therapy in recipients of ABO-incompatible kidney transplantation (ABOi-KT). \u0000 \u0000 \u0000Methods \u0000Retrospective analysis was conducted for e the clinical data of 40 ABOi-KT recipients between March 2017 and March 2019. 17 recipients of them received induction therapy with basiliximab (basiliximab group) while another 23 recipients received low-dose rATG (rATG group, rATG 25 mg/d×3 d). During a median follow-up period of 282 days, the data of serum creatinine and eGFR at 1 week and 1 month, graft survival rate and complication rate of two groups were compared. \u0000 \u0000 \u0000Results \u0000No significant difference existed in age, gender, dialytic modality/duration, blood groups of recipients, HLA mis-match, blood group antibody titers, dose of rituximab, blood groups of donors or donor age (P>0.05). The times of double filtration plasmapheresis in Basiliximab group were more (P 0.05). No significant difference existed in graft survival rate. No significant difference existed in rate of acute rejection, parvovirus B19 infection, urinary tract infection or hematoma. \u0000 \u0000 \u0000Conclusions \u0000Low-dose of rATG is as effective as basiliximab for ABOi-KT recipients. And rATG does not increase the rate of infection. \u0000 \u0000 \u0000Key words: \u0000Kidney transplantation; ABO blood group; Incompatible; Living donor; Rabbit antithymocyte globulin; Basiliximab","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89559302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ split liver transplantation for pediatric recipient: a single-center experience","authors":"Yuan Guo, Jin-Zhen Cai, Yi Luo, Zhiqiang Li, Ning Fan, Xin Wang, Ge Guan, Yandong Sun, Yang Xin, Chuansheng Xu, Jianhong Wang, Yunjin Zang","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.01.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.01.005","url":null,"abstract":"Objective \u0000To evaluate the efficacy of in-situ split liver transplantation (ISSLT) in children. \u0000 \u0000 \u0000Methods \u0000From June 2015 to August 2018, 10 liver grafts from DBD were split in-situ. All the donors were male, and the median age of the donors was 28.5 year old (18-48 year). One left half graft and 9 left lateral lobe grafts (including 2 reduced size grafts) were transplanted to 10 pediatric recipients. Four grafts were transplanted in our center, and the rest 6 grafts were shared to other two transplant center. The primary diseases of the recipients included biliary atresia (8/10), hepatic sinus obstruction syndrome (1/10) and Alagille syndrome (1/10). The median age of the recipients was 10 month (7 month-11 year), and the mean body weight was 9.8±6.6 kg (5-28 kg). \u0000 \u0000 \u0000Results \u0000All liver grafts were split in-situ. The mean split time of liver grafts was 88.5±18.9 min. The mean weight of split grafts was 336.7±85.4 g. All recipients were subjected to piggyback liver transplantation. Operation time was 542.5±112.1 min. Anhepatic time was 52.0±13.5 min. GRWR was (3.98±0.96)%. GRWR of two cases was more than 5%, so segment Ⅲ was partially reduced. During the follow-up period, 9 cases were alive and 1 case died due to multiple organ failure 1 day after liver transplantation. \u0000 \u0000 \u0000Conclusions \u0000ISSLT can enlarge the graft pool for children and achieve good results. \u0000 \u0000 \u0000Key words: \u0000Liver transplantation; In-situ split; Child","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74604097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}