Cell medicine最新文献_第4页

Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells on Concanavalin A-Induced Acute Liver Injury in Mice. 脂肪组织源性干细胞对豆豆蛋白a诱导的小鼠急性肝损伤的免疫调节作用。

Cell medicine Pub Date : 2017-01-01 DOI: 10.3727/215517916X693159

Yasuma Yoshizumi, H. Yukawa, Ryoji Iwaki, S. Fujinaka, A. Kanou, Yuki Kanou, Tatsuya Yamada, S. Nakagawa, Tomomi Ohara, Kenta Nakagiri, Y. Ogihara, Y. Tsutsui, Y. Hayashi, M. Ishigami, Y. Baba, Tetsuya Ishikawa

{"title":"Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells on Concanavalin A-Induced Acute Liver Injury in Mice.","authors":"Yasuma Yoshizumi, H. Yukawa, Ryoji Iwaki, S. Fujinaka, A. Kanou, Yuki Kanou, Tatsuya Yamada, S. Nakagawa, Tomomi Ohara, Kenta Nakagiri, Y. Ogihara, Y. Tsutsui, Y. Hayashi, M. Ishigami, Y. Baba, Tetsuya Ishikawa","doi":"10.3727/215517916X693159","DOIUrl":"https://doi.org/10.3727/215517916X693159","url":null,"abstract":"Cell therapy with adipose tissue-derived stem cells (ASCs) is expected to be a candidate for the treatment of fulminant hepatic failure (FHF), which is caused by excessive immune responses. In order to evaluate the therapeutic effects of ASCs on FHF, the in vitro and in vivo immunomodulatory effects of ASCs were examined in detail in the mouse model. The in vitro effects of ASCs were examined by assessing their influence on the proliferation of lymphomononuclear cells (LMCs) stimulated with three kinds of mitogens: phorbol 12-myristate 13-acetate (PMA) plus ionomycin, concanavalin A (ConA), and lipopolysaccharide (LPS). The proliferation of LMCs was efficiently suppressed in a dose-dependent manner by ASCs in the cases of PMA plus ionomycin stimulation and ConA stimulation, but not in the case of LPS stimulation. The in vivo effects of transplanted ASCs were examined in the murine FHF model induced by ConA administration. The ALT levels and histological inflammatory changes in the ConA-administered mice were apparently relieved by the transplantation of ASCs. The analysis of mRNA expression patterns in the livers indicated that the expressions of the cytokines such as Il-6, Il-10, Ifn-γ, and Tnf-α, and the cell surface markers such as Cd3γ, Cd4, Cd8α, Cd11b, and Cd11c were downregulated in the ASC-transplanted mice. The immunomodulatory and therapeutic effects of ASCs were confirmed in the mouse model both in vitro and in vivo. These suggest that the cell therapy with ASCs is beneficial for the treatment of FHF.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517916X693159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69756756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Facilitating Transplantation. 促进移植。

Cell medicine Pub Date : 2017-01-01 DOI: 10.3727/215517916X693078

H. Noguchi

引用次数: 0

Neurovascular Cell Sheet Transplantation in a Canine Model of Intracranial Hemorrhage. 神经血管细胞片移植在犬颅内出血模型中的应用。

Cell medicine Pub Date : 2016-12-21 eCollection Date: 2017-01-01 DOI: 10.3727/215517916X693384

Woo-Jin Lee, Jong Young Lee, Keun-Hwa Jung, Soon-Tae Lee, Hyo Yeol Kim, Dong-Kyu Park, Jung-Suk Yu, So-Yun Kim, Daejong Jeon, Manho Kim, Sang Kun Lee, Jae-Kyu Roh, Kon Chu

{"title":"Neurovascular Cell Sheet Transplantation in a Canine Model of Intracranial Hemorrhage.","authors":"Woo-Jin Lee, Jong Young Lee, Keun-Hwa Jung, Soon-Tae Lee, Hyo Yeol Kim, Dong-Kyu Park, Jung-Suk Yu, So-Yun Kim, Daejong Jeon, Manho Kim, Sang Kun Lee, Jae-Kyu Roh, Kon Chu","doi":"10.3727/215517916X693384","DOIUrl":"https://doi.org/10.3727/215517916X693384","url":null,"abstract":"Cell-based therapy for intracerebral hemorrhage (ICH) has a great therapeutic potential. However, methods to effectively induce direct regeneration of the damaged neural tissue after cell transplantation have not been established, which, if done, would improve the efficacy of cell-based therapy. In this study, we aimed to develop a cell sheet with neurovasculogenic potential and evaluate its usefulness in a canine ICH model. We designed a composite cell sheet made of neural progenitors derived from human olfactory neuroepithelium and vascular progenitors from human adipose tissue-derived stromal cells. We also generated a physiologic canine ICH model by manually injecting and then infusing autologous blood under arterial pressure. We transplanted the sheet cells (cell sheet group) or saline (control group) at the cortex over the hematoma at subacute stages (2 weeks from ICH induction). At 4 weeks from the cell transplantation, cell survival, migration, and differentiation were evaluated. Hemispheric atrophy and neurobehavioral recovery were also compared between the groups. As a result, the cell sheet was rich in extracellular matrices and expressed neurotrophic factors as well as the markers for neuronal development. After transplantation, the cells successfully survived for 4 weeks, and a large portion of those migrated to the perihematomal site and differentiated into neurons and pericytes (20% and 30% of migrated stem cells, respectively). Transplantation of cell sheets alleviated hemorrhage-related hemispheric atrophy (p = 0.042) and showed tendency for improving functional recovery (p = 0.062). Therefore, we concluded that the cell sheet transplantation technique might induce direct regeneration of neural tissue and might improve outcomes of intracerebral hemorrhage.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517916X693384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35174808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Higher Serum Alanine Transaminase Levels in Male Urokinase-Type Plasminogen Activator-Transgenic Mice Are Associated With Improved Engraftment of Hepatocytes but not Liver Sinusoidal Endothelial Cells. 雄性尿激酶型纤溶酶原激活物转基因小鼠血清谷丙转氨酶水平升高与肝细胞移植改善有关，但与肝窦内皮细胞移植改善无关。

Cell medicine Pub Date : 2016-11-23 eCollection Date: 2017-01-01 DOI: 10.3727/215517916X693375

Marina E Fomin, Ashley I Beyer, Jean Publicover, Kai Lu, Sonia Bakkour, Graham Simmons, Marcus O Muench

{"title":"Higher Serum Alanine Transaminase Levels in Male Urokinase-Type Plasminogen Activator-Transgenic Mice Are Associated With Improved Engraftment of Hepatocytes but not Liver Sinusoidal Endothelial Cells.","authors":"Marina E Fomin, Ashley I Beyer, Jean Publicover, Kai Lu, Sonia Bakkour, Graham Simmons, Marcus O Muench","doi":"10.3727/215517916X693375","DOIUrl":"https://doi.org/10.3727/215517916X693375","url":null,"abstract":"The effects of sex on the degree of liver damage and human cell engraftment were investigated in immunodeficient urokinase-type plasminogen activator-transgenic (uPA-NOG) mice. Liver damage, measured by serum alanine transaminase (ALT) levels, was compared in male and female uPA-NOG mice of different ages. Male mice had significantly higher ALT levels than females with a median of 334 versus 158 U/L in transgenic homozygous mice, respectively. Mice were transplanted with human adult hepatocytes or fetal liver cells and analyzed for any correlation of engraftment of hepatocytes, liver sinusoidal endothelial cells (LSECs), and hematopoietic cells with the degree of liver damage. Hepatocyte engraftment was measured by human albumin levels in the mouse serum. Higher ALT levels correlated with higher hepatocyte engraftment, resulting in albumin levels in male mice that were 9.6 times higher than in females. LSEC and hematopoietic cell engraftment were measured by flow cytometric analysis of the mouse liver and bone marrow. LSEC and hematopoietic engraftment did not differ between male and female transplant recipients. Thus, the sex of uPA-NOG mice affects the degree of liver damage, which is reflected in the levels of human hepatocyte engraftment. However, the high levels of LSEC engraftment observed in uPA-NOG mice are not further improved among male mice, suggesting that a lower threshold of liver damage is sufficient to enhance endothelial cell engraftment. Previously described sex differences in human hematopoietic stem cell engraftment in immunodeficient mice were not observed in this model.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517916X693375","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35174811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A Prospective, Nonrandomized, no Placebo-Controlled, Phase I/II Clinical Trial Assessing the Safety and Efficacy of Intramuscular Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells in Patients With Severe Buerger's Disease. 一项前瞻性、非随机、无安慰剂对照的I/II期临床试验，评估肌肉注射自体脂肪组织来源的间充质干细胞治疗严重伯格氏病的安全性和有效性。

Cell medicine Pub Date : 2016-10-03 eCollection Date: 2017-01-01 DOI: 10.3727/215517916X693069

Jeong Chan Ra, Euicheol C Jeong, Sung Keun Kang, Seog Ju Lee, Kyoung Ho Choi

{"title":"A Prospective, Nonrandomized, no Placebo-Controlled, Phase I/II Clinical Trial Assessing the Safety and Efficacy of Intramuscular Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells in Patients With Severe Buerger's Disease.","authors":"Jeong Chan Ra, Euicheol C Jeong, Sung Keun Kang, Seog Ju Lee, Kyoung Ho Choi","doi":"10.3727/215517916X693069","DOIUrl":"https://doi.org/10.3727/215517916X693069","url":null,"abstract":"Buerger's disease is a rare and severe disease affecting the blood vessels of the limbs. Adipose tissue-derived mesenchymal stem cells (ADSCs) have the potential to cure Buerger's disease when developed as a stem cell drug. In the present study, we conducted a prospective, nonrandomized, no placebo-controlled, phase I/II clinical trial with a 2-year follow-up questionnaire survey. A total of 17 patients were intramuscularly administered autologous ADSCs at a dose of 5 million cells/kg. The incidence of adverse events (AEs), adverse drug reaction (ADR), and serious adverse events (SAEs) was monitored. No ADRs and SAEs related to stem cell treatment occurred during the 6-month follow-up. In terms of efficacy, the primary endpoint was increase in total walking distance (TWD). The secondary endpoint was improvement in rest pain, increase in pain-free walking distance (PFWD), toe-brachial pressure index (TBPI), transcutaneous oxygen pressure (TcPO2), and arterial brachial pressure index (ABPI). ADSCs demonstrated significant functional improvement results including increased TWD, PFWD, and rest pain reduction. No amputations were reported during the 6-month clinical trial period and in the follow-up questionnaire survey more than 2 years after the ADSC injection. In conclusion, intramuscular injection of ADSCs is very safe and is shown to prompt functional improvement in patients with severe Buerger's disease at a dosage of 300 million cells per 60 kg of body weight. However, the confirmatory therapeutic efficacy and angiogenesis need further study.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517916X693069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35174809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

αSMA Expression in Large Colonies of Colony-Forming Units-Fibroblast as an Early Predictor of Bone Marrow MSC Expandability. αSMA在大集落形成单位中的表达-成纤维细胞作为骨髓间充质干细胞可扩展性的早期预测因子。

Cell medicine Pub Date : 2016-10-01 DOI: 10.3727/215517916X693357

I. Aizman, W. Holland, Cher Yang, D. Bates

引用次数: 4

Allogeneic Mesenchymal Stem Cell Transplantation in Dogs With Keratoconjunctivitis Sicca. 同种异体间充质干细胞移植治疗犬先天性角膜结膜炎。

Cell medicine Pub Date : 2016-10-01 DOI: 10.3727/215517916X693366

M. W. Bittencourt, M. A. Barros, J. P. F. Martins, J. P. Vasconcellos, B. Morais, C. Pompeia, Matheus Domingues Bittencourt, Karine Evangelho, I. Kerkis, C. Wenceslau

{"title":"Allogeneic Mesenchymal Stem Cell Transplantation in Dogs With Keratoconjunctivitis Sicca.","authors":"M. W. Bittencourt, M. A. Barros, J. P. F. Martins, J. P. Vasconcellos, B. Morais, C. Pompeia, Matheus Domingues Bittencourt, Karine Evangelho, I. Kerkis, C. Wenceslau","doi":"10.3727/215517916X693366","DOIUrl":"https://doi.org/10.3727/215517916X693366","url":null,"abstract":"Keratoconjunctivitis sicca (KCS) is a dysfunction in tear production associated with clinical signs, which include conjunctival hyperemia, ocular discharge, discomfort, pain, and, eventually, corneal vascularization and pigmentation. Immunosuppressive drugs are routinely administrated for long periods to treat KCS but with side effects and limited results. Evaluation of the clinical benefits of intralacrimal transplantation of allogeneic mesenchymal stem cells (MSCs) in dogs with mild-moderate and severe KCS was done. A total of 24 eyes with KCS from 15 dogs of different breeds were enrolled in the present study. A single transplantation of MSCs (1 × 106) directly into lacrimal glands (dorsal and third eyelid) was performed. The Schirmer tear tests (STTs) and ocular surface improvements were used to assess short- and long-term effects of these cells. The STTs were carried out on day 0 (before MSCs transplantation) and on days 7, 14, 21, and 28, as well as 6 and 12 months after MSC transplantation. Our data demonstrate that allogeneic MSC transplantation in KCS dogs is safe since no adverse effects were observed immediately after transplantation and in short- and long-term follow-ups. A statistically significant increase in the STT and ocular surface improvements was found in all eyes studied. In all the eyes with mild-moderate KCS, STT values reverted to those of healthy eyes, while in eyes with severe KCS, although complete reversion was not found, there was improvement in tear production and in other clinical signs. Our study shows that a single dose of a low number of MSCs can be used to treat KCS in dogs. In contrast to immunosuppressive drug use, MSC transplantation has an effect over a long period (up to 12 months), even after a single administration, and does not require daily drug administration.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517916X693366","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69756827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Measurement of DNA Length Changes Upon CpG Hypermethylation by Microfluidic Molecular Stretching. 微流控分子拉伸技术测量CpG超甲基化时DNA长度的变化。

Cell medicine Pub Date : 2016-09-14 eCollection Date: 2017-01-08 DOI: 10.3727/215517916X693087

Daisuke Onoshima, Naoko Kawakita, Daiki Takeshita, Hirohiko Niioka, Hiroshi Yukawa, Jun Miyake, Yoshinobu Baba

引用次数: 2

Comparison of Purification Solutions With Different Osmolality for Porcine Islet Purification. 不同渗透压纯化猪胰岛液的比较。

Cell medicine Pub Date : 2016-09-01 eCollection Date: 2017-01-08 DOI: 10.3727/215517916X693140

Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi

{"title":"Comparison of Purification Solutions With Different Osmolality for Porcine Islet Purification.","authors":"Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi","doi":"10.3727/215517916X693140","DOIUrl":"10.3727/215517916X693140","url":null,"abstract":"The osmolality of the purification solution is one of the most critical variables in human islet purification during islet isolation. We previously reported the effectiveness of a combined continuous density/osmolality gradient for the supplemental purification of human islets. We herein applied a combined continuous density/osmolality gradient for regular purification. The islets were purified with a continuous density gradient without osmolality preparation [continuous density/normal osmolality (CD/NO)] or continuous density/osmolality solution with osmolality preparation by 10× Hank's balanced salt solution (HBSS) [continuous density/continuous osmolality (CD/CO)]. The osmolality of the low-density solution was 400 mOsm/kg in both groups and that of the high-density solution was 410 mOsm/kg in the CD/NO group and 500 mOsm/kg in the CD/CO group. Unexpectedly, we noted no significant differences between the two solutions in terms of the islet yield, rate of viability and purity, score, stimulation index, or the attainability and suitability of posttransplantation normoglycemia. Despite reports that the endocrine and exocrine tissues of pancreata have distinct osmotic sensitivities and that high-osmolality solutions result in greater purification efficiency, the isolation and transplant outcomes did not markedly differ between the two purification solutions with different osmolalities in this study.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517916X693140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69756749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The Evaluation of Islet Purification Methods That Use Large Bottles to Create a Continuous Density Gradient. 利用大瓶产生连续密度梯度的胰岛净化方法的评价。

Cell medicine Pub Date : 2016-09-01 eCollection Date: 2017-01-08 DOI: 10.3727/215517916X693131

Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi

{"title":"The Evaluation of Islet Purification Methods That Use Large Bottles to Create a Continuous Density Gradient.","authors":"Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi","doi":"10.3727/215517916X693131","DOIUrl":"10.3727/215517916X693131","url":null,"abstract":"Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. The most common method of islet purification is density gradient centrifugation using a COBE 2991 cell processor. However, this method can damage islets mechanically through its high shearing force. We recently reported that a new purification method using large plastic bottles effectively achieves a high yield of islets from the porcine pancreas. In the present study, we evaluated the methods of making a continuous density gradient. The gradient was produced with a gradient maker and two types of candy cane-shaped stainless steel pipes. One method was to use a \"bent-tipped\" stainless steel pipe and to load from a high-density solution to a low-density solution, uploading the stainless steel pipe. The other method was to use a regular stainless steel pipe and to load from a low-density solution to a high-density solution, leaving the stainless steel pipe in place. There were no significant differences between the two solutions in terms of the islet yield, rate of viability or purity, score, or the stimulation index after purification. Furthermore, there were no differences in the attainability or suitability of posttransplantation normoglycemia. Our study shows the equivalency of these two methods of islet purification.","PeriodicalId":9780,"journal":{"name":"Cell medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/215517916X693131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69756481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9