不同渗透压纯化猪胰岛液的比较。

Cell medicine Pub Date : 2016-09-01 eCollection Date: 2017-01-08 DOI:10.3727/215517916X693140
Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi
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引用次数: 11

摘要

在胰岛分离过程中,纯化液的渗透压是胰岛净化过程中最关键的变量之一。我们之前报道了连续密度/渗透压梯度联合用于人胰岛补充净化的有效性。我们在此应用连续密度/渗透压梯度组合进行常规净化。采用连续密度梯度法纯化胰岛,不进行渗透压制备[连续密度/正常渗透压(CD/NO)]或连续密度/渗透压溶液(10倍汉克平衡盐溶液[连续密度/连续渗透压(CD/CO)]]进行渗透压制备。两组低密度溶液渗透压均为400 mOsm/kg, CD/NO组高密度溶液渗透压为410 mOsm/kg, CD/CO组渗透压为500 mOsm/kg。出乎意料的是,我们注意到两种解决方案在胰岛产量、存活率和纯度、评分、刺激指数或移植后正常血糖的可获得性和适宜性方面没有显著差异。尽管有报道称胰腺的内分泌和外分泌组织具有不同的渗透敏感性,高渗透压溶液的纯化效率更高,但在本研究中,不同渗透压的两种纯化溶液的分离和移植结果没有明显差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of Purification Solutions With Different Osmolality for Porcine Islet Purification.

The osmolality of the purification solution is one of the most critical variables in human islet purification during islet isolation. We previously reported the effectiveness of a combined continuous density/osmolality gradient for the supplemental purification of human islets. We herein applied a combined continuous density/osmolality gradient for regular purification. The islets were purified with a continuous density gradient without osmolality preparation [continuous density/normal osmolality (CD/NO)] or continuous density/osmolality solution with osmolality preparation by 10× Hank's balanced salt solution (HBSS) [continuous density/continuous osmolality (CD/CO)]. The osmolality of the low-density solution was 400 mOsm/kg in both groups and that of the high-density solution was 410 mOsm/kg in the CD/NO group and 500 mOsm/kg in the CD/CO group. Unexpectedly, we noted no significant differences between the two solutions in terms of the islet yield, rate of viability and purity, score, stimulation index, or the attainability and suitability of posttransplantation normoglycemia. Despite reports that the endocrine and exocrine tissues of pancreata have distinct osmotic sensitivities and that high-osmolality solutions result in greater purification efficiency, the isolation and transplant outcomes did not markedly differ between the two purification solutions with different osmolalities in this study.

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来源期刊
Cell medicine
Cell medicine MEDICINE, RESEARCH & EXPERIMENTAL-
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