Chemistry & biology最新文献

ADP-ribosylserine hydrolase ARH3 of Latimeria chalumnae in complex with ADP-ribosyl-L-arginine 与adp -核糖基- l-精氨酸配合物的紫堇adp -核糖基丝氨酸水解酶ARH3

Chemistry & biology Pub Date : 2018-11-28 DOI: 10.2210/PDB6HH4/PDB

J. Rack, Antonio Ayala Ariza, Bryon S. Drown, Callum Henfrey, Edward J. Bartlett, T. Shirai, P. Hergenrother, I. Ahel

{"title":"ADP-ribosylserine hydrolase ARH3 of Latimeria chalumnae in complex with ADP-ribosyl-L-arginine","authors":"J. Rack, Antonio Ayala Ariza, Bryon S. Drown, Callum Henfrey, Edward J. Bartlett, T. Shirai, P. Hergenrother, I. Ahel","doi":"10.2210/PDB6HH4/PDB","DOIUrl":"https://doi.org/10.2210/PDB6HH4/PDB","url":null,"abstract":"Summary Protein ADP-ribosylation is a highly dynamic post-translational modification. The rapid turnover is achieved, among others, by ADP-(ribosyl)hydrolases (ARHs), an ancient family of enzymes that reverses this modification. Recently ARHs came into focus due to their role as regulators of cellular stresses and tumor suppressors. Here we present a comprehensive structural analysis of the enzymatically active family members ARH1 and ARH3. These two enzymes have very distinct substrate requirements. Our data show that binding of the adenosine ribose moiety is highly diverged between the two enzymes, whereas the active sites harboring the distal ribose closely resemble each other. Despite this apparent similarity, we elucidate the structural basis for the selective inhibition of ARH3 by the ADP-ribose analogues ADP-HPD and arginine-ADP-ribose. Together, our biochemical and structural work provides important insights into the mode of enzyme-ligand interaction, helps to understand differences in their catalytic behavior, and provides useful tools for targeted drug design.","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"151 1","pages":"1533"},"PeriodicalIF":0.0,"publicationDate":"2018-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84296045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Human ISPD Is a Cytidyltransferase Required for Dystroglycan O-Mannosylation. 人ISPD是糖醛基化所必需的胞基转移酶。

Chemistry & biology Pub Date : 2015-12-17 DOI: 10.1016/j.chembiol.2015.10.014

M. Riemersma, D. S. Froese, Walinka van Tol, U. Engelke, J. Kopeć, Monique van Scherpenzeel, A. Ashikov, T. Krojer, Frank von Delft, M. Tessari, A. Buczkowska, E. Swiezewska, L. T. Jae, T. Brummelkamp, H. Manya, T. Endo, Hans van Bokhoven, W. Yue, D. Lefeber

引用次数: 61

Halophilic Protein Adaptation Results from Synergistic Residue-Ion Interactions in the Folded and Unfolded States. 在折叠和未折叠状态下，残基-离子的协同相互作用导致了嗜盐蛋白的适应性。

Chemistry & biology Pub Date : 2015-12-17 DOI: 10.1016/j.chembiol.2015.10.010

G. Ortega, T. Diercks, Ó. Millet

引用次数: 36

Differential Regulation of Specific Sphingolipids in Colon Cancer Cells during Staurosporine-Induced Apoptosis. staurosporin诱导结肠癌细胞凋亡过程中特定鞘脂的差异调控。

Chemistry & biology Pub Date : 2015-12-17 DOI: 10.1016/j.chembiol.2015.11.004

Virginia del Solar, Darleny Y. Lizardo, Nasi Li, Jerod J. Hurst, Christopher J. Brais, G. E. Atilla‐Gokcumen

引用次数: 40

Reciprocal Regulation of ERα and ERβ Stability and Activity by Diptoindonesin G. 双胞杆菌G对ERα和ERβ稳定性和活性的相互调控。

Chemistry & biology Pub Date : 2015-12-17 DOI: 10.1016/j.chembiol.2015.10.011

Zibo Zhao, Lu Wang, Youngeun Jung, Ikyon Kim, R. Tan, Wei Xu

引用次数: 32

Unraveling the B. pseudomallei Heptokinase WcbL: From Structure to Drug Discovery 假芽孢杆菌Heptokinase WcbL的揭示:从结构到药物发现

Chemistry & biology Pub Date : 2015-12-17 DOI: 10.1016/j.chembiol.2015.10.015

M. Vivoli, M. Isupov, R. Nicholas, A. Hill, Andrew E. Scott, P. Kosma, J. Prior, N. Harmer

引用次数: 7

Biosynthesis of Neocarazostatin A Reveals the Sequential Carbazole Prenylation and Hydroxylation in the Tailoring Steps. Neocarazostatin A的生物合成揭示了裁剪步骤中咔唑烯酰化和羟基化的顺序。

Chemistry & biology Pub Date : 2015-12-17 DOI: 10.1016/j.chembiol.2015.10.012

Sheng Huang, S. Elsayed, Meinan Lv, J. Tabudravu, M. Rateb, Roland Gyampoh, K. Kyeremeh, R. Ebel, M. Jaspars, Z. Deng, Yi-Zun Yu, Hai Deng

引用次数: 30

DIVERSE System: De Novo Creation of Peptide Tags for Non-enzymatic Covalent Labeling by In Vitro Evolution for Protein Imaging Inside Living Cells. 多样化的系统:重新创造肽标签的非酶共价标记通过体外进化蛋白质成像在活细胞内。

Chemistry & biology Pub Date : 2015-12-17 DOI: 10.1016/j.chembiol.2015.10.016

T. Kawakami, K. Ogawa, N. Goshima, T. Natsume

引用次数: 14

Synthetic Peptides as cGMP-Independent Activators of cGMP-Dependent Protein Kinase Iα. 合成肽作为cgmp依赖性蛋白激酶Iα的cgmp非依赖性激活剂。

Chemistry & biology Pub Date : 2015-12-17 DOI: 10.1016/j.chembiol.2015.11.005

T. M. Moon, N. Tykocki, Jessica L. Sheehe, B. W. Osborne, W. Tegge, J. Brayden, W. Dostmann

引用次数: 12

Unsaturated Lipid Assimilation by Mycobacteria Requires Auxiliary cis-trans Enoyl CoA Isomerase. 分枝杆菌的不饱和脂质同化需要辅助的顺-反式烯丙基辅酶a异构酶。

Chemistry & biology Pub Date : 2015-12-17 Epub Date: 2015-11-25 DOI: 10.1016/j.chembiol.2015.10.009

Sonali Srivastava, Sarika Chaudhary, Lipi Thukral, Ce Shi, Rinkoo D Gupta, Radhika Gupta, K Priyadarshan, Archana Vats, Asfarul S Haque, Rajan Sankaranarayanan, Vivek T Natarajan, Rakesh Sharma, Courtney C Aldrich, Rajesh S Gokhale

{"title":"Unsaturated Lipid Assimilation by Mycobacteria Requires Auxiliary cis-trans Enoyl CoA Isomerase.","authors":"Sonali Srivastava, Sarika Chaudhary, Lipi Thukral, Ce Shi, Rinkoo D Gupta, Radhika Gupta, K Priyadarshan, Archana Vats, Asfarul S Haque, Rajan Sankaranarayanan, Vivek T Natarajan, Rakesh Sharma, Courtney C Aldrich, Rajesh S Gokhale","doi":"10.1016/j.chembiol.2015.10.009","DOIUrl":"10.1016/j.chembiol.2015.10.009","url":null,"abstract":"<p><p>Mycobacterium tuberculosis (Mtb) can survive in hypoxic necrotic tissue by assimilating energy from host-derived fatty acids. While the expanded repertoire of β-oxidation auxiliary enzymes is considered crucial for Mtb adaptability, delineating their functional relevance has been challenging. Here, we show that the Mtb fatty acid degradation (FadAB) complex cannot selectively break down cis fatty acyl substrates. We demonstrate that the stereoselective binding of fatty acyl substrates in the Mtb FadB pocket is due to the steric hindrance from Phe287 residue. By developing a functional screen, we classify the family of Mtb Ech proteins as monofunctional or bifunctional enzymes, three of which complement the FadAB complex to degrade cis fatty acids. Crystal structure determination of two cis-trans enoyl coenzyme A (CoA) isomerases reveals distinct placement of active-site residue in Ech enzymes. Our studies thus reveal versatility of Mtb lipid-remodeling enzymes and identify an essential role of stand-alone cis-trans enoyl CoA isomerases in mycobacterial biology. </p>","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"19 1","pages":"1577-87"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89636208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0