Cellular and molecular bioengineering最新文献

{"title":"Effects of FTY720 on Neural Cell Behavior in Two and Three-Dimensional Culture and in Compression Spinal Cord Injury.","authors":"Zahra Zeraatpisheh, Fatemeh Shamsi, Parisa Sarkoohi, Somayyeh Torabi, Hamed Alipour, Hadi Aligholi","doi":"10.1007/s12195-022-00724-0","DOIUrl":"10.1007/s12195-022-00724-0","url":null,"abstract":"<p><strong>Introduction: </strong>The present study aimed to evaluate the effects of FTY720 as a neuromodulatory drug on the behaviors of neural stem/progenitor cells (NS/PCs) in two-dimensional (2-D) and three-dimensional (3-D) cultures and in spinal cord injury (SCI).</p><p><strong>Methods: </strong>The NS/PCs isolated from the ganglionic eminence of the 13.5-day old embryos were cultured as free-floating spheres. The single cells obtained from the second passage were cultured in 96-well plates without any scaffold (2-D) or containing PuraMatrix (PM, 3-D) or were used for transplantation in a mouse model of compression SCI. After exposure to 0, 10, 50, and 100 nanomolar of FTY720, the survival, proliferation, and migration of the NS/PCs were evaluated <i>in vitro</i> using MTT assay, neurosphere assay, and migration assay, respectively. Moreover, the functional recovery, survival and migration capacity of transplanted cells exposure to 100 nanomolar FTY720 were investigated in SCI.</p><p><strong>Results: </strong>Cell survival and migration capacity increased after exposure to 50 and 100 nanomolar FTY720. In addition, higher doses of FTY720 led to the formation of more extensive and more neurospheres. Although this phenomenon was similar in both 2-D and 3-D cultures, PM induced better distribution of the cells in a 3-D environment. Furthermore, co-administration of FTY720 and NS/PCs 7 days after SCI enhanced functional recovery and both survival and migration of transplanted cells in the lesion site.</p><p><strong>Conclusions: </strong>Due to the positive effects of FTY720 on the behavior of NS/PCs, using them in combination therapies can be an appealing approach for stem cell therapy in CNS injury.</p>","PeriodicalId":9687,"journal":{"name":"Cellular and molecular bioengineering","volume":"15 4","pages":"331-340"},"PeriodicalIF":2.3,"publicationDate":"2022-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474962/pdf/12195_2022_Article_724.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9614784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Novel Technique for the Measurement of Neuromuscular Junction Functionality in Isotonic Conditions","authors":"Flavia Forconi, L. Apa, S. Pisu, I. Casola, A. Musarò, E. Rizzuto, Z. Del Prete","doi":"10.1007/s12195-022-00721-3","DOIUrl":"https://doi.org/10.1007/s12195-022-00721-3","url":null,"abstract":"","PeriodicalId":9687,"journal":{"name":"Cellular and molecular bioengineering","volume":"15 1","pages":"255 - 265"},"PeriodicalIF":2.8,"publicationDate":"2022-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45330910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Regional Variations of the Interstitial Cells of Cajal in the Murine Distal Stomach Informed by Confocal Imaging and Machine Learning Methods.","authors":"Sue Ann Mah,&nbsp;Peng Du,&nbsp;Recep Avci,&nbsp;Jean-Marie Vanderwinden,&nbsp;Leo K Cheng","doi":"10.1007/s12195-021-00716-6","DOIUrl":"https://doi.org/10.1007/s12195-021-00716-6","url":null,"abstract":"<p><strong>Introduction: </strong>The network of Interstitial Cells of Cajal (ICC) plays a plethora of key roles in maintaining, coordinating, and regulating the contractions of the gastrointestinal (GI) smooth muscles. Several GI functional motility disorders have been associated with ICC degradation. This study extended a previously reported 2D morphological analysis and applied it to 3D spatial quantification of three different types of ICC networks in the distal stomach guided by confocal imaging and machine learning methods. The characterization of the complex changes in spatial structure of the ICC network architecture contributes to our understanding of the roles that different types of ICC may play in post-prandial physiology, pathogenesis, and/or amelioration of GI dsymotility- bridging structure and function.</p><p><strong>Methods: </strong>A validated classification method using Trainable Weka Segmentation was applied to segment the ICC from a confocal dataset of the gastric antrum of a transgenic mouse, followed by structural analysis of the segmented images.</p><p><strong>Results: </strong>The machine learning model performance was compared to manually segmented subfields, achieving an area under the receiver-operating characteristic (AUROC) of 0.973 and 0.995 for myenteric ICC (ICC-MP; <i>n </i>= 6) and intramuscular ICC (ICC-IM; <i>n </i>= 17). The myenteric layer in the distal antrum increased in thickness (from 14.5 to 34 <i>μ</i>m) towards the lesser curvature, whereas the thickness decreased towards the lesser curvature in the proximal antrum (17.7 to 9 <i>μ</i>m). There was an increase in ICC-MP volume from proximal to distal antrum (406,960 ± 140,040 vs. 559,990 ± 281,000 <i>μ</i>m³; <i>p </i>= 0.000145). The % of ICC volume was similar for ICC-LM and for ICC-CM between proximal (3.6 ± 2.3% vs. 3.1 ± 1.2%; <i>p </i>= 0.185) and distal antrum (3.2 ± 3.9% vs. 2.5 ± 2.8%; <i>p</i> = 0.309). The average % volume of ICC-MP was significantly higher than ICC-IM at all points throughout sample (<i>p </i>< 0.0001).</p><p><strong>Conclusions: </strong>The segmentation and analysis methods provide a high-throughput framework of investigating the structural changes in extended ICC networks and their associated physiological functions in animal models.</p>","PeriodicalId":9687,"journal":{"name":"Cellular and molecular bioengineering","volume":"15 2","pages":"193-205"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938532/pdf/12195_2021_Article_716.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10463255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sevoflurane Suppresses the Proliferation, Migration and Invasion of Colorectal Cancer Through Regulating Circ_0000423/miR-525-5p/SGPP1 Network.","authors":"Xiaofang Kang,&nbsp;Xiaocong Li,&nbsp;Yanli Li","doi":"10.1007/s12195-021-00717-5","DOIUrl":"https://doi.org/10.1007/s12195-021-00717-5","url":null,"abstract":"<p><strong>Introduction: </strong>Sevoflurane (SEV) has been shown to inhibit the malignant progression in many cancers, including colorectal cancer (CRC). However, it is not clear whether SEV regulates the progression of CRC by mediating the circular RNA (circRNA) axis.</p><p><strong>Methods: </strong>Different concentrations of SEV were used to treat CRC cells. Cell proliferation, migration and invasion were determined by cell counting kit 8 assay, colony formation assay and transwell assay. The expression of circ_0000423, microRNA (miR)-525-5p and sphingosine-1-phosphate phosphatase 1 (SGPP1) mRNA was measured by quantitative real-time PCR. Cell apoptosis was assessed using flow cytometry, and protein expression was measured by western blot analysis. Dual-luciferase reporter assay and RIP assay were performed to confirm the interactions among circ_0000423, miR-525-5p and SGPP1. Animal experiments were performed to explore the effect of SEV and circ_0000423 on CRC tumorigenesis.</p><p><strong>Results: </strong>SEV could inhibit CRC cell proliferation, migration and invasion. Circ_0000423 was upregulated in CRC and its expression could be reduced by SEV. Overexpressed circ_0000423 reversed the inhibitory effect of SEV on CRC cell proliferation, migration and invasion and the promotion effect on cell apoptosis. MiR-525-5p could be sponged by circ_0000423, and its overexpression also abolished the regulation of circ_0000423 on the progression of SEV-treated CRC cells. In addition, SGPP1 was confirmed to be a target of miR-525-5p, and its expression was positively regulated by circ_0000423. MiR-525-5p inhibitor promoted CRC cell progression under the treatment of SEV, while these effects could be overturned by SGPP1 silencing. Furthermore, the inhibition effect of SEV on CRC tumorigenesis also could be abolished by overexpressing circ_0000423.</p><p><strong>Conclusion: </strong>Our results showed that SEV inhibited CRC progression through the regulation of circ_0000423/miR-525-5p/SGPP1 axis.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12195-021-00717-5.</p>","PeriodicalId":9687,"journal":{"name":"Cellular and molecular bioengineering","volume":"15 2","pages":"219-230"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938590/pdf/12195_2021_Article_717.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9120302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Pregnancy-Specific Glycoproteins on Trophoblast Motility in Three-Dimensional Gelatin Hydrogels.","authors":"Samantha G Zambuto,&nbsp;Shemona Rattila,&nbsp;Gabriela Dveksler,&nbsp;Brendan A C Harley","doi":"10.1007/s12195-021-00715-7","DOIUrl":"https://doi.org/10.1007/s12195-021-00715-7","url":null,"abstract":"<p><strong>Introduction: </strong>Trophoblast invasion is a complex biological process necessary for establishment of pregnancy; however, much remains unknown regarding what signaling factors coordinate the extent of invasion. Pregnancy-specific glycoproteins (PSGs) are some of the most abundant circulating trophoblastic proteins in maternal blood during human pregnancy, with maternal serum concentrations rising to as high as 200-400 μg/mL at term.</p><p><strong>Methods: </strong>Here, we employ three-dimensional (3D) trophoblast motility assays consisting of trophoblast spheroids encapsulated in 3D gelatin hydrogels to quantify trophoblast outgrowth area, viability, and cytotoxicity in the presence of PSG1 and PSG9 as well as epidermal growth factor and Nodal.</p><p><strong>Results: </strong>We show PSG9 reduces trophoblast motility whereas PSG1 increases motility. Further, we assess bulk nascent protein production by encapsulated spheroids to highlight the potential of this approach to assess trophoblast response (motility, remodeling) to soluble factors and extracellular matrix cues.</p><p><strong>Conclusions: </strong>Such models provide an important platform to develop a deeper understanding of early pregnancy.</p>","PeriodicalId":9687,"journal":{"name":"Cellular and molecular bioengineering","volume":"15 2","pages":"175-191"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938592/pdf/12195_2021_Article_715.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9180091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Albumin-Binding Aptamer Chimeras for Improved siRNA Bioavailability.","authors":"Jonah C Rosch,&nbsp;Ella N Hoogenboezem,&nbsp;Alexander G Sorets,&nbsp;Craig L Duvall,&nbsp;Ethan S Lippmann","doi":"10.1007/s12195-022-00718-y","DOIUrl":"https://doi.org/10.1007/s12195-022-00718-y","url":null,"abstract":"<p><strong>Introduction: </strong>Short interfering RNAs (siRNAs) are potent nucleic acid-based drugs designed to target disease driving genes that may otherwise be undruggable with small molecules. However, therapeutic potential of siRNA <i>in vivo</i> is limited by poor pharmacokinetic properties, including rapid renal clearance and nuclease degradation. Backpacking on natural carriers such as albumin, which is present at high concentration and has a long half-life in serum, is an effective way to modify pharmacokinetics of biologic drugs that otherwise have poor bioavailability. In this work, we sought to develop albumin-binding aptamer-siRNA chimeras to improve the bioavailability of siRNA.</p><p><strong>Methods: </strong>A Systematic Evolution of Ligands through Exponential Enrichment (SELEX) approach was used to obtain modified RNA-binding aptamers, which were then fused directly to siRNA via <i>in vitro</i> transcription. Molecular and pharmacokinetic properties of the aptamer-siRNA chimeras were subsequently measured <i>in vitro</i> and <i>in vivo</i>.</p><p><strong>Results: </strong><i>In vitro</i> assays show that albumin-binding aptamers are stable in serum while maintaining potent gene knockdown capabilities in the chimera format. <i>In vivo</i>, the absolute circulation half-life of the best-performing aptamer-siRNA chimera (Clone 1) was 1.6-fold higher than a scrambled aptamer chimera control.</p><p><strong>Conclusions: </strong>Aptamer-siRNA chimeras exhibit improved bioavailability without compromising biological activity. Hence, this albumin-binding aptamer-siRNA chimera approach may be a promising strategy for drug delivery applications.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12195-022-00718-y.</p>","PeriodicalId":9687,"journal":{"name":"Cellular and molecular bioengineering","volume":"15 2","pages":"161-173"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938549/pdf/12195_2022_Article_718.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9447581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minimally-Invasive and Efficient Method to Accurately Fit the Bergman Minimal Model to Diabetes Type 2.","authors":"Ana Gabriela Gallardo-Hernández, Marcos A González-Olvera, Medardo Castellanos-Fuentes, Jésica Escobar, Cristina Revilla-Monsalve, Ana Luisa Hernandez-Perez, Ron Leder","doi":"10.1007/s12195-022-00719-x","DOIUrl":"10.1007/s12195-022-00719-x","url":null,"abstract":"<p><strong>Introduction: </strong>Diabetes mellitus is a global burden that is expected to grow <math><mrow><mn>25</mn> <mo>%</mo></mrow> </math> by 2030. This will increase the need for prevention, diagnosis and treatment of diabetes. Animal and individualized <i>in silico</i> models will allow understanding and compensation for inter and intra-individual differences in treatment and management strategies for diabetic patients. The method presented here can advance the concept of personalized medicine.</p><p><strong>Methods: </strong>Twenty experiments were performed with Sprague-Dawley rats with streptozotocin induced experimental diabetes in which the insulin-glucose response curve was recorded over 60-100 min using only an insulin pump and a percutaneous glucose sensor. The information was used to fit the five-parameter Bergman Minimal Model to the experimental results using a genetic algorithm with a root-mean-squared optimization rule.</p><p><strong>Results: </strong>The Bergman Minimal Model parameters were estimated with high accuracy, low prediction bias, and low average root-mean-squared error of 15.27 mg/dl glucose.</p><p><strong>Conclusions: </strong>This study demonstrates a simple method to accurately parameterize the Bergman Minimal Model. We used Sprague-Dawley rats since their physiology is close to that of humans. The parameters can be used to objectively characterize the physiological severity of diabetes. In this way, planned treatments can compensate for natural variations of conditions both inter and intra patients. Changes in parameters indicate the patient's diabetic condition using values of glucose effectiveness ( <math> <mrow><msub><mi>S</mi> <mtext>G</mtext></msub> <mo>=</mo> <msub><mi>p</mi> <mn>1</mn></msub> </mrow> </math> ) and insulin sensitivity ( <math> <mrow><msub><mi>S</mi> <mi>I</mi></msub> <mo>=</mo> <msub><mi>p</mi> <mn>3</mn></msub> <mo>/</mo> <msub><mi>p</mi> <mn>2</mn></msub> </mrow> </math> ). Quantifying the diabetic patient's condition is consistent with the trend toward personalized medicine. Parameter values can also be used to explain atypical research results of other studies and increase understanding of diabetes.</p>","PeriodicalId":9687,"journal":{"name":"Cellular and molecular bioengineering","volume":"15 3","pages":"267-279"},"PeriodicalIF":2.3,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124285/pdf/12195_2022_Article_719.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9157912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LINC00852 Regulates Cell Proliferation, Invasion, Migration and Apoptosis in Hepatocellular Carcinoma Via the miR-625/E2F1 Axis","authors":"Shi Chen","doi":"10.1007/s12195-021-00714-8","DOIUrl":"https://doi.org/10.1007/s12195-021-00714-8","url":null,"abstract":"","PeriodicalId":9687,"journal":{"name":"Cellular and molecular bioengineering","volume":"15 1","pages":"207 - 217"},"PeriodicalIF":2.8,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44710433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Objective Assessment of Covid-19 Severity Affecting the Vocal and Respiratory System Using a Wearable, Autonomous Sound Collar.","authors":"D Ishac,&nbsp;S Matta,&nbsp;S Bin,&nbsp;H Aziz,&nbsp;E Karam,&nbsp;A Abche,&nbsp;G Nassar","doi":"10.1007/s12195-021-00712-w","DOIUrl":"https://doi.org/10.1007/s12195-021-00712-w","url":null,"abstract":"<p><strong>Introduction: </strong>Since the outbreak began in January 2020, Covid-19 has affected more than 161 million people worldwide and resulted in about 3.3 million deaths. Despite efforts to detect human infection with the virus as early as possible, the confirmatory test still requires the analysis of sputum or blood with estimated results available within approximately 30 minutes; this may potentially be followed by clinical referral if the patient shows signs of aggravated pneumonia. This work aims to implement a soft collar as a sound device dedicated to the objective evaluation of the pathophysiological state resulting from dysphonia of laryngeal origin or respiratory failure of inflammatory origin, in particular caused by Covid-19.</p><p><strong>Methods: </strong>In this study, we exploit the vibrations of waves generated by the vocal and respiratory system of 30 people. A biocompatible acoustic sensor embedded in a soft collar around the neck collects these waves. The collar is also equipped with thermal sensors and a cross-data analysis module in both the temporal and frequency domains (STFT). The optimal coupling conditions and the electrical and dimensional characteristics of the sensors were defined based on a mathematical approach using a matrix formalism.</p><p><strong>Results: </strong>The characteristics of the signals in the time domain combined with the quantities obtained from the STFT offer multidimensional information and a decision support tool for determining a pathophysiological state representative of the symptoms explored. The device, tested on 30 people, was able to differentiate patients with mild symptoms from those who had developed acute signs of respiratory failure on a severity scale of 1 to 10.</p><p><strong>Conclusion: </strong>With the health constraints imposed by the effects of Covid-19, the heavy organization to be implemented resulting from the flow of diagnostics, tests and clinical management, it was urgent to develop innovative and safe biomedical technologies. This passive listening technique will contribute to the non-invasive assessment and dynamic observation of lesions. Moreover, it merits further examination to provide support for medical operators to improve clinical management.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12195-021-00712-w.</p>","PeriodicalId":9687,"journal":{"name":"Cellular and molecular bioengineering","volume":"15 1","pages":"67-86"},"PeriodicalIF":2.8,"publicationDate":"2021-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39732044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Atherosclerotic Plaque Coating for Thrombosis Microfluidics Assays.","authors":"M F A Karel,&nbsp;T P Lemmens,&nbsp;B M E Tullemans,&nbsp;S J H Wielders,&nbsp;E Gubbins,&nbsp;D van Beurden,&nbsp;S van Rijt,&nbsp;J M E M Cosemans","doi":"10.1007/s12195-021-00713-9","DOIUrl":"https://doi.org/10.1007/s12195-021-00713-9","url":null,"abstract":"<p><strong>Introduction: </strong>Studying arterial thrombus formation by <i>in vitro</i> flow assays is a widely used approach. Incorporating human atherosclerotic plaque material as a thrombogenic surface in these assays represents a method to model the pathophysiological environment of thrombus formation upon plaque disruption. Up until now, achieving a homogeneous coating of plaque material and subsequent reproducible platelet adhesion has been challenging. Here, we characterized a novel method for coating of plaque material on glass coverslips for use in thrombosis microfluidic assays.</p><p><strong>Methods: </strong>A homogenate of human atherosclerotic plaques was coated on glass coverslips by conventional manual droplet coating or by spin coating. Prior to coating, a subset of coverslips was plasma treated. Water contact angle measurements were performed as an indicator for the hydrophilicity of the coverslips. Homogeneity of plaque coatings was determined using profilometric analysis and scanning electron microscopy. Thrombogenicity of the plaque material was assessed in real time by microscopic imaging while perfusing whole blood at a shear rate of 1500 s^-1 over the plaque material.</p><p><strong>Results: </strong>Plasma treatment of glass coverslips, prior to spin coating with plaque material, increased the hydrophilicity of the coverslip compared to no plasma treatment. The most homogeneous plaque coating and highest platelet adhesion was obtained upon plasma treatment followed by spin coating of the plaque material. Manual plaque coating on non-plasma treated coverslips yielded lowest coating homogeneity and platelet adhesion and activation.</p><p><strong>Conclusion: </strong>Spin coating of atherosclerotic plaque material on plasma treated coverslips leads to a more homogenous coating and improved platelet adhesion to the plaque when compared to conventional droplet coating on non-plasma treated coverslips. These properties are beneficial in ensuring the quality and reproducibility of flow experiments.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12195-021-00713-9.</p>","PeriodicalId":9687,"journal":{"name":"Cellular and molecular bioengineering","volume":"15 1","pages":"55-65"},"PeriodicalIF":2.8,"publicationDate":"2021-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39964343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}