Cell Cycle最新文献_第10页

Investigating the role of LncRNA PSMG3-AS1 in gastric cancer: implications for prognosis and therapeutic intervention. 研究LncRNA PSMG3-AS1在癌症中的作用：对预后和治疗干预的影响。

IF 3.4 3区生物学

Cell Cycle Pub Date : 2023-10-01 Epub Date: 2023-12-05 DOI: 10.1080/15384101.2023.2278942

Yi Wang, Zhongshi Hong, Shenghong Wei, Zaisheng Ye, Luchuan Chen, Chengzhi Qiu

{"title":"Investigating the role of LncRNA PSMG3-AS1 in gastric cancer: implications for prognosis and therapeutic intervention.","authors":"Yi Wang, Zhongshi Hong, Shenghong Wei, Zaisheng Ye, Luchuan Chen, Chengzhi Qiu","doi":"10.1080/15384101.2023.2278942","DOIUrl":"10.1080/15384101.2023.2278942","url":null,"abstract":"LncRNAs are widely linked to the complex development of gastric cancer, which is acknowledged worldwide as the third highest contributor to cancer-related deaths and the fifth most common form of cancer. The primary focus of this study is to examine the role of LncRNA PSMG3-AS1 in a group of individuals with gastric cancer. The results of our study indicate that PSMG3-AS1 is highly expressed in over 20 different types of cancer. Significantly, there was a clear association found between the expression of PSMG3-AS1 and a multitude of TMB and MSI tumors. PSMG3-AS1 exhibited significant upregulation in gastric cancer patients compared to healthy individuals within the gastric cancer cohort. The prognosis of gastric cancer patients is intrinsically associated with PSMG3-AS1, as confirmed by survival analysis and ROC curves. Furthermore, we created a disruption vector based on LncRNA PSMG3-AS1 and introduced it into AGS and MKN-45 cells, which are human gastric cancer cells. Significant decreases in the expression of the PSMG3-AS1 gene were noticed in both intervention groups compared to the NC group, reflecting the protein level expressions. Significantly, the proliferative and invasive capabilities of MKN-45 and AGS cells were notably reduced following transfection with PSMG3-AS1 siRNA. The results of our study indicate that disruption of the LncRNA PSMG3-AS1 gene may impact the CAV1/miR-451a signaling pathway, thereby leading to a reduction in the ability of gastric cancer cells to multiply and invade.","PeriodicalId":9686,"journal":{"name":"Cell Cycle","volume":" ","pages":"2161-2171"},"PeriodicalIF":3.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10732658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72013626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and functional insights into GSDMB isoforms complex roles in pathogenesis. GSDMB异构体在发病机制中的复杂作用的结构和功能见解。

IF 3.4 3区生物学

Cell Cycle Pub Date : 2023-10-01 Epub Date: 2023-12-15 DOI: 10.1080/15384101.2023.2287933

Sara Colomo, David Ros-Pardo, Sara S Oltra, Paulino Gomez-Puertas, David Sarrio, Gema Moreno-Bueno

{"title":"Structural and functional insights into GSDMB isoforms complex roles in pathogenesis.","authors":"Sara Colomo, David Ros-Pardo, Sara S Oltra, Paulino Gomez-Puertas, David Sarrio, Gema Moreno-Bueno","doi":"10.1080/15384101.2023.2287933","DOIUrl":"10.1080/15384101.2023.2287933","url":null,"abstract":"SHADSGasdermins (GSDMs) have garnered significant scientific interest due to their protective and detrimental roles in innate immunity, host defense, inflammation, and cancer alongside with other pathologies. While GSDMs are mostly recognized as key effectors of a lytic type of pro-inflammatory cell death known as pyroptosis, they do also take part in other cell death processes (NETosis, secondary necrosis, or apoptosis) and exhibit cell-death independent functions depending on the cellular context. Among GSDMs, Gasdermin B (GSDMB) pyroptotic capacity has been a subject of conflicting findings in scientific literature even when its processing, and subsequent activation, by Granzyme A (GZMA) was decoded. Nevertheless, recent groundbreaking publications have shed light on the crucial role of alternative splicing in determining the pyroptotic capacity of GSDMB isoforms, which depends on the presence of exon 6-derived elements. This comprehensive review pays attention to the relevant structural differences among recently crystalized GSDMB isoforms. As a novelty, the structural aspects governing GSDMB isoform susceptibility to GZMA-mediated activation have been investigated. By elucidating the complex roles of GSDMB isoforms, this review aims to deepen the understanding of this multifunctional player and its potential implications in disease pathogenesis and therapeutic interventions. [Figure: see text].","PeriodicalId":9686,"journal":{"name":"Cell Cycle","volume":" ","pages":"2346-2359"},"PeriodicalIF":3.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of HNRNPM inhibits the progression of glioma through inducing ferroptosis. 敲低HNRNPM通过诱导铁下垂抑制胶质瘤的进展。

IF 3.4 3区生物学

Cell Cycle Pub Date : 2023-10-01 Epub Date: 2023-12-15 DOI: 10.1080/15384101.2023.2286782

Jian Wang, Xiaolin Luo, Dehua Liu

{"title":"Knockdown of HNRNPM inhibits the progression of glioma through inducing ferroptosis.","authors":"Jian Wang, Xiaolin Luo, Dehua Liu","doi":"10.1080/15384101.2023.2286782","DOIUrl":"10.1080/15384101.2023.2286782","url":null,"abstract":"Purpose: Ferroptosis acts as an important regulator in diverse human tumors, including the glioma. This study aimed to screen potential ferroptosis-related genes involved in the progression of glioma.Materials and methods: Differently expressed genes (DEGs) were screened based on GSE31262 and GSE12657 datasets, and ferroptosis-related genes were separated. Among the important hub genes in the protein-protein interaction networks, HNRNPM was selected as a research target. Following the knockdown of HNRNPM, the viability, migration, and invasion were detected by CCK8, wound healing, and transwell assays, respectively. The role of HNRNPM knockdown was also verified in a xenograft tumor model in mice. Immunohistochemistry detected the expression levels of HNRNPM and Ki67. Moreover, the ferroptosis was evaluated according to the levels of iron, glutathione peroxidase (GSH), and malondialdehyde (MDA), as well as the expression of PTGS2, GPX4, and FTH1.Results: Total 41 overlapping DEGs relating with ferroptosis and glioma were screened, among which 4 up-regulated hub genes (HNRNPM, HNRNPA3, RUVBL1, and SNRPPF) were determined. The up-regulation of HNRNPM presented a certain predictive value for glioma. In addition, knockdown of HNRNPM inhibited the viability, migration, and invasion of glioma cells in vitro, and also the tumor growth in mice. Notably, knockdown of HNRNPM enhanced the ferroptosis in glioma cells. Furthermore, HNRNPM was positively associated with SMARCA4 in glioma.Conclusions: Knockdown of HNRNPM inhibits the progression of glioma via inducing ferroptosis. HNRNPM is a promising molecular target for the treatment of glioma via inducing ferroptosis. We provided new insights of glioma progression and potential therapeutic guidance.","PeriodicalId":9686,"journal":{"name":"Cell Cycle","volume":" ","pages":"2264-2279"},"PeriodicalIF":3.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138450988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of LOX gene G473A polymorphism with the occurrence of allergic rhinitis and efficacy of montelukast sodium in children. LOX基因G473A多态性与儿童变应性鼻炎发生及孟鲁司特钠疗效的关系

IF 3.4 3区生物学

Cell Cycle Pub Date : 2023-10-01 Epub Date: 2023-12-15 DOI: 10.1080/15384101.2023.2286802

Xikun Yao, Yan Liu, Hong Jiao, Wenjie Ma, Minliang Chen

{"title":"Association of LOX gene G473A polymorphism with the occurrence of allergic rhinitis and efficacy of montelukast sodium in children.","authors":"Xikun Yao, Yan Liu, Hong Jiao, Wenjie Ma, Minliang Chen","doi":"10.1080/15384101.2023.2286802","DOIUrl":"10.1080/15384101.2023.2286802","url":null,"abstract":"Allergic rhinitis (AR) is very common in adolescents, and current treatment options are complex and unsatisfactory. The objective of this study was to analyze the association of lysyl oxidase (LOX) gene G473A polymorphism with susceptibility to AR in children. In addition, we analyzed the therapeutic effect of montelukast sodium on AR. Forty-five children with AR (research group, 8.16±2.88 years old) and 51 healthy children (control group, 8.22±3.87 years old) during the same period were selected. The LOX gene G473A polymorphism was detected with polymerase chain reaction (PCR)-restriction fragment length polymorphism method. The effect of G473A polymorphism in the occurrence of AR was assessed by logistic regression analysis. In addition, the levels of C-reactive protein (CRP), Interleukin (IL-6), and IL-8 were measured to observe the relationship between G473A polymorphism and inflammatory factors. Finally, montelukast sodium was given to children with AR to investigate the effect of G473A polymorphism on clinical outcomes. The number of G473A polymorphisms in the research group was not significantly different from the control group for GA-type (P = 0.521). However, the number of GG-type polymorphisms was less while the number of type AA was more than the control group (P = 0.044 and 0.046). Children carrying the AA gene had an approximately 4-fold increased risk of AR, while those carrying the GG gene had a decreased risk (P < 0.001). Moreover, children carrying the GG gene had lower levels of CRP, IL-6, and IL-8 and better clinical outcomes, while those carrying the AA gene had higher levels of inflammatory factors and worse outcomes (P<0.05). LOX gene G473A polymorphism is closely associated with AR pathogenesis and may have an important research value in antagonizing the therapeutic effect of montelukast sodium.","PeriodicalId":9686,"journal":{"name":"Cell Cycle","volume":" ","pages":"2280-2287"},"PeriodicalIF":3.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Krüppel-like factor 5 activates chick intestinal stem cell and promotes mucosal repair after impairment. kr<s:1> ppel样因子5激活鸡肠干细胞，促进损伤后黏膜修复。

IF 3.4 3区生物学

Cell Cycle Pub Date : 2023-10-01 Epub Date: 2023-12-05 DOI: 10.1080/15384101.2023.2278938

Lingzi Yu, Sichao Qi, Guozhen Wei, Xi Rao, Danni Luo, Minyao Zou, Yuling Mi, Caiqiao Zhang, Jian Li

{"title":"Krüppel-like factor 5 activates chick intestinal stem cell and promotes mucosal repair after impairment.","authors":"Lingzi Yu, Sichao Qi, Guozhen Wei, Xi Rao, Danni Luo, Minyao Zou, Yuling Mi, Caiqiao Zhang, Jian Li","doi":"10.1080/15384101.2023.2278938","DOIUrl":"10.1080/15384101.2023.2278938","url":null,"abstract":"The mucosal renewal, which depends on the intestinal stem cell (ISC) activity, is the foundation of mucosal repairment. Importantly, activation of reserve ISCs (rISCs) plays a vital role in initiating mucosal repair after injury. However, the underlying regulatory mechanism of rISCs activation in chickens remains unclear. In this study, immediately after lipopolysaccharide (LPS) challenge, mitochondrial morphological destruction and dysfunction appeared in the crypt, accompanied by decreased epithelial secretion (decreased Muc2 mRNA abundance and LYSOZYME protein level). However, immediately after mucosal injury, the mucosal renewal accelerated, as indicated by the increased BrdU positive rate, proliferating cell nuclear antigen (PCNA) protein level and mRNA abundance of cell cycle markers (Ccnd1, Cdk2). Concerning the ISCs activity, during the early period of injury, there appeared a reduction of active ISCs (aISCs) marker Lgr5 mRNA and protein, and an increasing of rISCs marker Hopx mRNA and protein. Strikingly, upon LPS challenge, increased mRNA transcriptional level of Krüppel-like factor 5 (Klf5) was detected in the crypt. Moreover, under LPS treatment in organoids, the KLF5 inhibitor (ML264) would decrease the mRNA and protein levels of Stat5a and Hopx, the STAT5A inhibitor (AC-4-130) would suppress the Lgr5 mRNA and protein levels. Furthermore, the Dual-Luciferase Reporter assay confirmed that, KLF5 would bind to Hopx promoter and activate the rISCs, STAT5A would trigger Lgr5 promoter and activate the aISCs. Collectively, KLF5 was upregulated during the early period of injury, further activate the rISCs directly and activate aISCs via STAT5A indirectly, thus initiate mucosal repair after injury.","PeriodicalId":9686,"journal":{"name":"Cell Cycle","volume":" ","pages":"2142-2160"},"PeriodicalIF":3.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10732631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89717019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epididymal segment-specific miRNA and mRNA regulatory network at the single cell level. 附睾片段特异性miRNA和单细胞水平的mRNA调控网络。

IF 3.4 3区生物学

Cell Cycle Pub Date : 2023-10-01 Epub Date: 2023-12-05 DOI: 10.1080/15384101.2023.2280170

Tong Chen, Liangyu Yao, Wen Liu, Jiaochen Luan, Yichun Wang, Chao Yang, Xiang Zhou, Chengjian Ji, Xuejiang Guo, Zengjun Wang, Ninghong Song

{"title":"Epididymal segment-specific miRNA and mRNA regulatory network at the single cell level.","authors":"Tong Chen, Liangyu Yao, Wen Liu, Jiaochen Luan, Yichun Wang, Chao Yang, Xiang Zhou, Chengjian Ji, Xuejiang Guo, Zengjun Wang, Ninghong Song","doi":"10.1080/15384101.2023.2280170","DOIUrl":"10.1080/15384101.2023.2280170","url":null,"abstract":"Spermatozoa released from the testis cannot fertilize an egg before becoming mature and motile in the epididymis. Based on three bulk and one single-cell RNA-seq (scRNA-seq) data series, we compared mRNA or miRNA expression between epididymal segment-specific samples and the other samples. Hereby, we identified 570 differentially expressed mRNAs (DE-mRNAs) and 23 differentially expressed miRNAs (DE-miRNAs) in the caput, 175 DE-mRNAs and 15 DE-miRNAs in the corpus, 946 DE-mRNAs and 12 DE-miRNAs in the cauda. In accordance with respective DE-miRNAs, we predicted upstream transcription factors (TFs) and downstream target genes. Subsequently, we intersected target genes of respective DE-miRNAs with corresponding DE-mRNAs, thereby obtaining 127 upregulated genes in the caput and 92 upregulated genes in cauda. Enriched upregulated pathways included cell motility-related pathways for the caput, smooth muscle-related pathways for the corpus, and immune-associated pathways for the cauda. Protein-protein interaction (PPI) network was constructed to extract key module for the caput and cauda, followed by identifying hub genes through cytohubba. Epididymis tissues from six mice were applied to validate hub genes expression using qRT-PCR, and 7 of the 10 genes displayed identical expression trends in mice caput/cauda. These hub genes were found to be predominantly distributed in spermatozoa using scRNA-seq data. In addition, target genes of DE-miRNAs were intersected with genes in the PPI network for each segment. Subsequently, the miRNA and mRNA regulatory networks for the caput and cauda were constructed. Conclusively, we uncover segment-specific miRNA-mRNA regulatory network, upstream TFs, and downstream pathways of the human epididymis, warranting further investigation into epididymal segment-specific functions.","PeriodicalId":9686,"journal":{"name":"Cell Cycle","volume":" ","pages":"2194-2209"},"PeriodicalIF":3.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10732646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138046212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics integration analysis unveils heterogeneity in breast cancer at the individual level. 多组学整合分析揭示了个体水平乳腺癌的异质性。

IF 4.3 3区生物学

Cell Cycle Pub Date : 2023-10-01 Epub Date: 2023-12-15 DOI: 10.1080/15384101.2023.2281816

Zhangxiang Zhao, Tongzhu Jin, Bo Chen, Qi Dong, Mingyue Liu, Jiayu Guo, Xiaoying Song, Yawei Li, Tingting Chen, Huiming Han, Haihai Liang, Yunyan Gu

{"title":"Multi-omics integration analysis unveils heterogeneity in breast cancer at the individual level.","authors":"Zhangxiang Zhao, Tongzhu Jin, Bo Chen, Qi Dong, Mingyue Liu, Jiayu Guo, Xiaoying Song, Yawei Li, Tingting Chen, Huiming Han, Haihai Liang, Yunyan Gu","doi":"10.1080/15384101.2023.2281816","DOIUrl":"10.1080/15384101.2023.2281816","url":null,"abstract":"Identifying robust breast cancer subtypes will help to reveal the cancer heterogeneity. However, previous breast cancer subtypes were based on population-level quantitative gene expression, which is affected by batch effects and cannot be applied to individuals. We detected differential gene expression, genomic, and epigenomic alterations to identify driver differential expression at the individual level. The individual driver differential expression reflected the breast cancer patients' heterogeneity and revealed four subtypes. Mesenchymal subtype as the most aggressive subtype harbored deletion and downregulated expression of genes in chromosome 11q23 region. Specifically, silencing of the SDHD gene in 11q23 promoted the invasion and migration of breast cancer cells in vitro by the epithelial-mesenchymal transition. The immunologically hot subtype displayed an immune-hot microenvironment, including high T-cell infiltration and upregulated PD-1 and CTLA4. Luminal and genomic-unstable subtypes showed opposite macrophage polarization, which may be regulated by the ligand-receptor pairs of CD99. The integration of multi-omics data at the individual level provides a powerful framework for elucidating the heterogeneity of breast cancer.","PeriodicalId":9686,"journal":{"name":"Cell Cycle","volume":" ","pages":"2229-2244"},"PeriodicalIF":4.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136396569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lung adenocarcinoma presenting with intrapulmonary metastases through air spaces concomitant with silicosis: a case report and literature review. 肺腺癌伴矽肺经肺腔转移:1例报告及文献复习。

IF 3.4 3区生物学

Cell Cycle Pub Date : 2023-10-01 Epub Date: 2023-12-05 DOI: 10.1080/15384101.2023.2277512

Guzong Wang, Wenbin Hu, Binjun He, Yanhong Ma

{"title":"Lung adenocarcinoma presenting with intrapulmonary metastases through air spaces concomitant with silicosis: a case report and literature review.","authors":"Guzong Wang, Wenbin Hu, Binjun He, Yanhong Ma","doi":"10.1080/15384101.2023.2277512","DOIUrl":"10.1080/15384101.2023.2277512","url":null,"abstract":"Herein, we reported a rare case of bilateral intrapulmonary metastases spread through air spaces (STAS) and silicosis to advance understanding and knowledge of this disease. A middle-aged man was diagnosed with a left upper lung nodule with bilateral silicosis by preoperative imaging. Local pleural indentation and extensive metastases spread in the visceral pleura were observed during the operation. Pathological examination showed multiple metastases of lung adenocarcinoma, and STAS positive. Genetic testing indicated EGFR mutation, and ektinib was administered. STAS can promote lung cancer, leading to multiple pulmonary metastases, and silicosis can contribute to the carcinogenesis of lung cancer. This case provided valuable clinical lessons. More studies are warranted to elucidate the role and underlying mechanism of silicosis and STAS in the development of lung cancer. More accurate imaging methods and radiographic criteria should be formulated for different diffuse nodules and STAS grades, and the exploration of optimal therapeutic regimens to treat these concomitant patients is urgently needed to improve diagnostic rates and formulate more optimal therapies.","PeriodicalId":9686,"journal":{"name":"Cell Cycle","volume":" ","pages":"2113-2118"},"PeriodicalIF":3.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10732644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89717020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role and mechanism of ferroptosis in acute lung injury. 脱铁性贫血在急性肺损伤中的作用及机制。

IF 4.3 3区生物学

Cell Cycle Pub Date : 2023-10-01 Epub Date: 2023-12-05 DOI: 10.1080/15384101.2023.2278328

Tingting Yu, Shibo Sun

引用次数: 0

IGF2BP2 promotes glycolysis and hepatocellular carcinoma stemness by stabilizing CDC45 mRNA via m6A modification. IGF2BP2通过m6A修饰稳定CDC45 mRNA，促进糖酵解和肝细胞癌的发生。

IF 3.4 3区生物学

Cell Cycle Pub Date : 2023-10-01 Epub Date: 2023-12-15 DOI: 10.1080/15384101.2023.2283328

Tao Wu, Li Liao, Tao Wu, Shuai Chen, Qilin Yi, Min Xu

{"title":"IGF2BP2 promotes glycolysis and hepatocellular carcinoma stemness by stabilizing CDC45 mRNA via m6A modification.","authors":"Tao Wu, Li Liao, Tao Wu, Shuai Chen, Qilin Yi, Min Xu","doi":"10.1080/15384101.2023.2283328","DOIUrl":"10.1080/15384101.2023.2283328","url":null,"abstract":"A growing number of studies have shown the prognostic importance of Cell division cycle protein 45 (CDC45) in hepatocellular carcinoma (HCC). This study aims to investigate the biological function and mechanism of CDC45 in HCC. The differential expression and prognostic significance of CDC45 in HCC and normal tissues were analyzed by bioinformatics. CDC45 was knocked down and the biological effects of CDC45 in HCC in vitro and in vivo were measured. Subsequently, using RNA m6A colorimetry and Methylated RNA Immunoprecipitation (MeRIP), the levels of m6A modification of total RNA and CDC45 were evaluated in cells. RIP was applied to establish that CDC45 and insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) interact. A test using actinomycin D was performed to gauge the stability of the CDC45 mRNA. Furthermore, the regulatory role of IGF2BP2 on CDC45 expression in HCC progression was explored by overexpressing IGF2BP2. High expression of CDC45 was correlated with poor prognosis in HCC patients. Knocking down CDC45 inhibited HCC cell proliferation, migration, invasion, EMT, stemness, and glycolysis, and promoted apoptosis, which was verified through in vitro experiments. Additionally, IGF2BP2 was highly expressed in HCC cells, and it was found to interact with CDC45. Knocking down IGF2BP2 resulted in reduced stability of CDC45 mRNA. Moreover, overexpression of IGF2BP2 promoted HCC cell proliferation, migration, invasion, EMT, stemness, and glycolysis, while inhibiting apoptosis, which was reversed by knocking down CDC45. In general, IGF2BP2 promoted HCC glycolysis and stemness by stabilizing CDC45 mRNA via m6A modification. [Figure: see text].","PeriodicalId":9686,"journal":{"name":"Cell Cycle","volume":" ","pages":"2245-2263"},"PeriodicalIF":3.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138175723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0