Calcified Tissue International最新文献

{"title":"Enamel and Bleaching or Breaching: Vickers Hardness and Backscattered Electron Imaging.","authors":"Alan Boyde, Shima Ashrafianbonab, David Mills","doi":"10.1007/s00223-026-01518-6","DOIUrl":"10.1007/s00223-026-01518-6","url":null,"abstract":"","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13043528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147590345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can Difficulty Standing from a Chair Signal Early Risk of Chronic Illness? Insights from a Multi-Wave European Cohort.","authors":"M Azhar Hussain, Salma Naheed, Khalid Saeed, Asima Karim, Firdos Ahmad, Rizwan Qaisar, Shaea A Alkahtani","doi":"10.1007/s00223-026-01523-9","DOIUrl":"https://doi.org/10.1007/s00223-026-01523-9","url":null,"abstract":"","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147590327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor Radiotherapy-Induced Sympathetic Hyperactivation Orchestrates Rapid Systemic Bone Loss.","authors":"Chang Wang, Shouxiang Kuang, Lipeng Sun, Yang Li, Guodong Wang, Jianmin Sun, Fengge Zhou, Chenggui Zhang","doi":"10.1007/s00223-026-01514-w","DOIUrl":"https://doi.org/10.1007/s00223-026-01514-w","url":null,"abstract":"<p><p>Radiation therapy (RT) is widely used in cancer treatment but is associated with an increased risk of fractures, with underlying mechanisms remaining unclear. Here, we investigated RT-induced bone loss in an esophageal carcinoma mouse model and examined the role of the sympathetic nervous system (SNS). A total of 90 male C57BL/6J mice were employed in this study. Mice were subcutaneously inoculated with esophageal carcinoma cells and treated with localized RT (20 Gy in four fractions) with or without chemical sympathectomy. RT markedly increased norepinephrine levels and tyrosine hydroxylase expression in bone, accompanied by upregulation of β1-adrenergic receptor and β2-adrenergic receptor. In addition, RT induced increases in inflammatory markers in the vertebrae, and all these changes were attenuated by 6-OHDA. Micro-CT analysis revealed significant trabecular bone loss in the vertebrae, femur, and tibia following RT, as indicated by reduced BV/TV, whereas sympathectomy substantially mitigated bone loss. Histological and molecular analyses showed that RT increased osteoclast activity and osteocyte apoptosis while suppressing osteoblast function; these changes were largely reversed by 6-OHDA. In addition, RT reduced tumor volume, and sympathetic ablation further enhanced this antitumor effect. In summary, our results suggest that localized radiotherapy may induce systemic bone loss by triggering excessive activation of the SNS. Its potential mechanism may be related to increased osteocyte apoptosis, enhanced osteoclastogenesis, and impaired osteoblast activity. Therefore, targeting the SNS may represent a potentially effective strategy for preventing radiation-associated bone loss.</p>","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation Between the Deltoid Tuberosity Index and DXA Measurements: Feasibility of Opportunistic Osteoporosis Screening Using Routine Radiograph.","authors":"Nuri Koray Ülgen, Ali Said Nazlıgül, Nihat Yiğit, Mehmet Orçun Akkurt","doi":"10.1007/s00223-026-01519-5","DOIUrl":"https://doi.org/10.1007/s00223-026-01519-5","url":null,"abstract":"<p><p>Osteoporosis is a common metabolic bone disease associated with increased fracture risk and reduced quality of life. The deltoid tuberosity index (DTI), derived from humeral radiographs, reflects cortical thickness and may serve as a simple radiographic indicator of reduced bone mass. However, limited data exist regarding the relationship between DTI and dual-energy X-ray absorptiometry (DXA) parameters across different skeletal regions, particularly using routine chest radiographs. This retrospective study included 318 patients (303 females, 15 males) who underwent DXA measurements and radiographic imaging of the proximal humerus (chest, shoulder, cervical, or humeral radiographs). DTI measurements were independently performed by four observers, and mean values were used for analysis. Associations between DTI, bone mineral density (BMD), and T-scores were evaluated using Pearson correlation analysis. Receiver operating characteristic (ROC) analysis was performed to assess the ability of DTI to predict osteoporosis. DTI showed significant positive correlations with BMD and T-scores in both the lumbar spine (r = 0.295, p < 0.001) and femoral neck (r = 0.525, p < 0.001), with stronger associations observed in the femoral region. The area under the ROC curve (AUC) for predicting osteoporosis was 0.768 for the femoral neck and 0.677 for the lumbar spine. The optimal femoral DTI threshold for identifying femoral osteoporosis was 1.37 (sensitivity 73%, specificity 75%).Lumbar and femoral measurements were strongly correlated (BMD: r = 0.682; T-scores: r = 0.742; both p < 0.001), although femoral T-scores were significantly lower than lumbar values (mean difference = 0.17, p < 0.001). Across all age groups, DTI remained positively correlated with T-scores, with the strongest associations observed in patients aged ≥ 65 years. Interobserver reliability was excellent (intraclass correlation coefficient = 0.92). DTI is a practical, reproducible, and cost-effective radiographic parameter associated with reduced bone mass. Because it can be obtained from routinely performed chest radiographs, one of the most frequently used imaging examinations, DTI may help opportunistically identify individuals, particularly postmenopausal women, who could benefit from further osteoporosis evaluation and referral for formal bone mineral density assessment.Level of Evidence III: Diagnostic Study.</p>","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amelogenin Phosphorylation Affects Key Regulatory Genes in the Enamel Organ.","authors":"Brent Vasquez, Ai Thu Bui, Priya Mullick, Lasya Bhogadi, Henry C Margolis, Elia Beniash","doi":"10.1007/s00223-026-01516-8","DOIUrl":"https://doi.org/10.1007/s00223-026-01516-8","url":null,"abstract":"","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Large-Scale Multi-omics Polygenic Risk Score Analysis Identified Candidate Biomarkers Associated with Heel Bone Mineral Density.","authors":"Xuena Yang, Huan Liu, Ke Xu, Dan He, Shiqiang Cheng, Chuyu Pan, Li Liu, Wenming Wei, Boyue Zhao, Jingni Hui, Yan Wen, Yumeng Jia, Bolun Cheng, Peng Xu, Feng Zhang","doi":"10.1007/s00223-026-01494-x","DOIUrl":"https://doi.org/10.1007/s00223-026-01494-x","url":null,"abstract":"<p><strong>Objectives: </strong>Bone mineral density (BMD) is a critical indicator of osteoporosis (OP). Utilizing the latest multi-omics quantitative trait loci (QTLs) data, we aim to identify novel candidates associated with heel BMD (hBMD).</p><p><strong>Methods: </strong>We collected QTLs data from the INTERVAL cohort (independent of the UK Biobank (UKB)) that is a randomised trial including approximately 50,000 healthy blood donors enrolled from 25 centres of England's National Health Service Blood and Transplant. We then calculated individual polygenic risk score (PRS) for 13,646 RNAs, 308 proteins (Olink), 2379 proteins (SomaScan), 726 metabolites (Metabolon) and 141 metabolites (Nightingale) in UKB. hBMD was measured by quantitative ultrasound. Generalized linear model was used to evaluate the associations between multi-omics PRS and hBMD in 96,165 subjects. Integrated analysis of multi-omics was performed on the MetaboAnalyst 6.0 platform. Replication analysis was performed using the internal Olink proteomics and Nightingale metabolomics data of UKB. We subsequently explored the causal effects of the targets on hBMD using mendelian randomization (MR) analysis. Significant associations were determined using a false discovery rate (FDR)-adjusted P-value (Padj < 0.05).</p><p><strong>Results: </strong>We identified 195 hBMD-associated genes, such as WNT16 (Padj = 6.417 × 10^- 14); 180 proteins such as COL1A1 (Padj = 3.132 × 10^- 24); 21 metabolites, such as total cholesterol (Padj = 0.008). Those associated proteins/metabolites were replicated in UKB, such as COL1A1 (Padj = 7.480 × 10^- 4) and total cholesterol (Padj = 7.773 × 10^- 8). The integrated analysis of multi-omics identified several genes/metabolites (DGKZ, PLPP3, GPD1L, CHKB and glycerol 3-phosphate) enriched in the overlapping pathway-glycerophospholipid metabolism. Moreover, MR detected several novel biomarkers among the top/enriched targets, which were causally associated with hBMD, such as NAP1L2 (β= - 0.019, P = 0.003) and PLPP3 (β = - 0.026, P = 0.041).</p><p><strong>Conclusion: </strong>Our study not only identified several novel candidate biomarkers such as PLPP3, RGMB, and RNF128 for hBMD but also provided genetic evidence supporting their potential causal roles in bone mineral regulation. These findings could shed light on the molecular underpinnings of OP.</p>","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Course of Neuropsychological Functions in Chronic Non-Surgical Hypoparathyroidism on Conventional Therapy: 12 Years of Follow-Up.","authors":"Preeti Namjoshi, Snehaa Seetharaman, Soma Saha, Mani Kalaivani, Manjari Tripathi, Devasenathipathy Kandasamy, Sameer Aggarwal, Rajesh Sagar, Ravinder Goswami","doi":"10.1007/s00223-026-01507-9","DOIUrl":"https://doi.org/10.1007/s00223-026-01507-9","url":null,"abstract":"<p><strong>Purpose: </strong>Neuropsychological manifestations are common in chronic hypoparathyroidism (cHypoPT). Here, we report the change in cognitive and neuropsychiatric functions in cHypoPT during long-term follow-up.</p><p><strong>Design: </strong>Observational-cohort study.</p><p><strong>Methods: </strong>Fifty-four patients with non-surgical idiopathic cHypoPT (M: F = 24:30, age = 47.8 ± 14.0 years, duration of hypoparathyroidism = 24.5 ± 7.7 years), who were evaluated for cognitive, neuropsychiatric function, and quality of life in 2011-12, were re-evaluated in 2023-24 using the same battery of tests. Change in intracranial calcification was compared with previous computed tomography scans. Multivariable regression was used to assess association of change in cognitive and neuropsychiatric function with sex, age, body mass index (BMI), sites of intracranial calcification, and time weighted estimated glomerular filtration rate (eGFR), serum total calcium, phosphate, their product, and ratio.</p><p><strong>Results: </strong>The mean global cognitive dysfunction score increased during follow-up (4.04 ± 2.56 to 5.69 ± 2.10, p < 0.001). Motor speed and fine control, memory, trail-making test, Bender-Gestalt test, and quality of life showed significant deterioration. Deterioration in cognition was associated with increased age (p < 0.001). Neuropsychiatric assessment showed significant improvement in somatic concerns, anxiety, tension, and hostility. Male patient, increasing age, and higher BMI showed significant association with improvement in neuropsychiatric function. However, duration of illness and lower eGFR were associated with neuropsychiatric deterioration (p = 0.003 and 0.035). Changes in intracranial-calcification and biochemical control showed no significant association with cognitive and neuropsychiatric functions.</p><p><strong>Conclusions: </strong>Hypoparathyroid patients showed a significant decline in cognitive function during follow-up. However, neuropsychiatric function improved during follow-up, more so in males, in older individuals and in those with a higher BMI. Deteriorating cognition could affect the earning and compliance with treatment. Further studies assessing the role of behavioral/drug therapy would be helpful in cHypoPT.</p>","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Bayesian BMD-Derived Genome-Wide Polygenic Scores Enhance Clinical FRAX-Based Fracture Risk Prediction in Postmenopausal Women.","authors":"Anqi Liu, Jianing Liu, Qing Wu","doi":"10.1007/s00223-026-01497-8","DOIUrl":"10.1007/s00223-026-01497-8","url":null,"abstract":"<p><p>Current fracture prediction tools, including the Fracture Risk Assessment Tool (FRAX), rely primarily on clinical risk factors and bone mineral density measurements, yet omit genetic information. Given osteoporosis's substantial heritability (estimated at 50-80%), excluding genetic data may lead to clinical misclassification and uncertainty, particularly among postmenopausal women near clinical intervention thresholds. This study aimed to enhance clinical fracture risk prediction by integrating advanced Bayesian-derived genome-wide polygenic scores (GPS) into FRAX (Bayesian GPS-FRAX). Using genetic data from the Genetic Factors for Osteoporosis Consortium (GEFOS), we derived GPS using two advanced Bayesian methods, Polygenic Risk Score Continuous Shrinkage (PRS-CS) and Summary-data-based Bayesian Regression (SBayesR), selected for their ability to model complex genetic architectures and linkage disequilibrium. Bayesian GPS was integrated into FRAX, creating the Bayesian GPS-FRAX model, which was subsequently validated in 6932 postmenopausal women from the Women's Health Initiative. We evaluated model performance in terms of both discrimination and clinical utility, with clinical utility primarily assessed using net reclassification improvement (NRI), threshold-based clinical reclassification, and decision curve analysis. Robustness and generalizability were confirmed through independent validation in a separate cohort of 3688 women. Bayesian GPS-FRAX modestly improved discrimination, increasing the time-dependent AUC from 0.72 to 0.74, and yielded clinically meaningful gains in risk classification, with a NRI of 4.55-5.07% compared with FRAX-CRF. Approximately one-third of reclassified individuals had baseline FRAX scores within 15-25%, a critical zone of clinical uncertainty. Bayesian GPS-FRAX successfully reclassified these women, correcting misclassification and enabling more accurate and timely clinical interventions. The greatest reclassification benefit was evident among women older than 70. Decision curve analyses demonstrated consistent net clinical benefit across clinically relevant thresholds (15-25%), notably at the widely used 20% treatment threshold. Independent validation further confirmed robust generalizability and clinical applicability. Integrating Bayesian GPS into FRAX substantially enhances discrimination and clinical fracture risk stratification, especially in older women and individuals near critical intervention thresholds. By addressing genomic complexity and clinical uncertainty through advanced Bayesian methodologies, Bayesian GPS-FRAX represents a meaningful advancement toward personalized osteoporosis management. Prospective validation in diverse cohorts, along with assessments of clinical implementation and cost-effectiveness, will be essential to facilitate the integration of this precision-based approach into routine clinical practice.</p>","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13013207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ageing and Inequality Drive the Global Burden of Low Bone Mineral Density in Midlife and Older Women.","authors":"Mixue Guo, Meixuan Guo, Mengyuan Cai, Tao Sun, Lichun Qiao, Huqiang Dong","doi":"10.1007/s00223-026-01513-x","DOIUrl":"https://doi.org/10.1007/s00223-026-01513-x","url":null,"abstract":"","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pregnancy and Lactation Associated Osteoporosis: A Systematic Review.","authors":"Zeinab Issa, Randa Saad, Younes Lama, Shahed Ammar, Vanessa Akiki, Christopher S Kovacs, Ghada El-Hajj Fuleihan, Marlene Chakhtoura","doi":"10.1007/s00223-026-01499-6","DOIUrl":"https://doi.org/10.1007/s00223-026-01499-6","url":null,"abstract":"<p><p>Pregnancy and lactation-associated osteoporosis (PLO) is a rare disease. It represents a challenge for physicians for diagnosis, treatment and follow up. Our aim is to review fracture data in women with PLO presenting with fragility fractures occurring during late pregnancy or the postpartum/lactation period. We conducted a comprehensive literature search of the following databases: PubMed, MEDLINE and EMBASE from inception until October 2024. We included observational studies and placebo arms of interventional studies assessing fragility fractures at any site among reproductive-age women (18-50 years) during pregnancy, lactation, or up to one year postpartum. We abstracted data and assessed the quality of the included studies in duplicate and independently. We identified 11,375 citations in total, screened 329 full text articles, and included 17 papers from 12 original articles. The mean ± standard deviation (SD) age of women with PLO and fractures in the included studies ranged from 30.6 ± 3.3 to 39.5 ± 6 years. The mean ± SD BMI at the time of diagnosis ranged from 20.9 ± 1.9 to 23.9 ± 3.7 kg/m². In women with PLO, there was no clear association with the age at menarche, while being primiparous seemed a common yet inconsistent finding. While most reported fractures occur during lactation, evidence on whether lactation characteristics such as intensity, duration, and exclusivity modify fracture risk remains inconclusive. Around one third of women with PLO have family history of osteoporosis. Vertebral fractures are the most common fractures, specifically in the thoracolumbar spine. The mean ± SD number of vertebral fractures ranged from 3.3 ± 3.4 to 5.5 ± 3.3 per patient. The risk of fracture recurrence in subsequent pregnancies ranged between 7 and 28% in the included cohorts. We identified limited data on the beneficial impact and adverse events of anti-resorptive and anabolic therapy in women with PLO. PLO is a debilitating disorder in young women with scarce data on predictors and for evidence-based treatment strategies. Further prospective studies with control groups are needed.</p>","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}