Cancer biochemistry biophysics最新文献_第2页

Fluorescence spectroscopy of normal, SV40-transformed human keratinocytes, and carcinoma cells. 正常、sv40转化的人角质形成细胞和癌细胞的荧光光谱。

Cancer biochemistry biophysics Pub Date : 1999-07-01

A J Papadopoulos, N N Zhadin, M L Steinberg, R R Alfano

引用次数: 0

Modulation of the impaired drug metabolism in sarcoma-180-bearing mice by echitamine chloride. 氯echitamine对180肉瘤小鼠药物代谢受损的调节作用。

Cancer biochemistry biophysics Pub Date : 1999-07-01

V Saraswathi, V Mathuram, S Subramanian, S Govindasamy

引用次数: 0

Lipid peroxidation and antioxidant system in the blood of cancerous patients with metastasis. 肿瘤转移患者血液中脂质过氧化及抗氧化系统的研究。

Cancer biochemistry biophysics Pub Date : 1999-07-01

M Guven, B Ozturk, A Sayal, A Ozeturk, T Ulutin

引用次数: 0

Development of intracytoplasmic lumens in a colon cancer cell line cultured on a non-adhesive surface. 在非粘附表面培养的结肠癌细胞系细胞浆内腔的发育。

Cancer biochemistry biophysics Pub Date : 1999-07-01

S I Bae, G H Kang, Y I Kim, B L Lee, H K Kleinman, W H Kim

{"title":"Development of intracytoplasmic lumens in a colon cancer cell line cultured on a non-adhesive surface.","authors":"S I Bae, G H Kang, Y I Kim, B L Lee, H K Kleinman, W H Kim","doi":"","DOIUrl":"","url":null,"abstract":"Cell-matrix interactions have important effects on phenotypic features, such as morphology, differentiation and cell growth. Several papers have suggested that when cell-matrix interactions are interrupted, cells grow as multicellular spheroids and eventually undergo apoptosis. We found that when ET(-), a laminin non-adherent colon cancer cell line, was cultured on poly-2-hydroxyethyl methacrylate (HEMA) coated plastic, the cells floated as cellular aggregates of spheroids or as single cells. Some of the single cells contained a very large intracytoplasmic lumen (ICL) and appeared similar to signet ring cells. These ICL were lined by a layer of short microvilli. The number of the cell did not increased cells when cultured on poly-HEMA. Another type of single cells, usually without ICL, demonstrated the characteristics of apoptotic cells by histologic examination. Acridine orange staining, flow cytometry and electron microscopy confirmed the apoptotic nature of those cells. In immunohistochemical staining for proliferating cell nuclear antigen, spheroids of cells and single cells with ICL were immunoreactive, while most of the single cells without ICL were negative. These results suggest that multicellular aggregation and formation of ICL were induced by the adaptation of ET(-) colon cancer cells in a harmful environment caused by reduced adhesiveness, and these changes might be related to cell survival.","PeriodicalId":9552,"journal":{"name":"Cancer biochemistry biophysics","volume":"17 1-2","pages":"35-47"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21590596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total acid soluble and insoluble carnitine levels in human brain tumors. 人脑肿瘤中总酸溶性和不溶性肉碱水平。

Cancer biochemistry biophysics Pub Date : 1999-07-01

K S Sandikci, M K Gümüstaş, Y Tüter, E Kökoğlu, E Ozyurt, V Sözer

{"title":"Total acid soluble and insoluble carnitine levels in human brain tumors.","authors":"K S Sandikci, M K Gümüstaş, Y Tüter, E Kökoğlu, E Ozyurt, V Sözer","doi":"","DOIUrl":"","url":null,"abstract":"Carnitine has two main functions, i.e., transporting long-chain fatty acids into the mitochondrial matrix for beta-oxidation to provide cellular energy and modulating the rise in intramitochondrial acyl-CoA/CoA ratio, which relieves the inhibition of many intramitochondrial enzymes involving glucose and amino acid catabolism. The present study examined the acid soluble carnitine (ASCAR) acid insoluble carnitine (AICAR) and total carnitine (TCAR) concentrations of 50 human brain tumor tissues and 11 normal brain tissues. The ASCAR levels significantly higher in gliomas and meningiomas than brain, however similar to brain in metastatic adenocarcinomas. AICAR levels were lower than brain in all tumors with the exception of a medullablastoma. TCAR levels were similar to brain in all tumor types. Decreased AICAR levels may be due to increased utilization of lipids or enhanced phospholipid and cholesterol synthesis which is need for increased membrane synthesis or formation of eicosanoids. Also decreased concentrations may be a reflection of camitine and its acylesters role in preserving the physiologic membrane structure function from oxidative damage.","PeriodicalId":9552,"journal":{"name":"Cancer biochemistry biophysics","volume":"17 1-2","pages":"49-57"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21590597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effect of combined administration with CPT-11 and 5-fluorouracil in vitro and in vivo. CPT-11与5-氟尿嘧啶联合给药的体内外抑制作用。

Cancer biochemistry biophysics Pub Date : 1999-07-01

H Satoh, M Ohtomo, H Ishikawa, H Kamma, M Ohtsuka, S Hasegawa

引用次数: 0

The effects of reverse micelles on the reaction mechanism of alpha-chymotrypsin. 反胶束对α -凝乳胰蛋白酶反应机制的影响。

Cancer biochemistry biophysics Pub Date : 1998-11-01

P M D'Agostino, S K Chattopadhyay

{"title":"The effects of reverse micelles on the reaction mechanism of alpha-chymotrypsin.","authors":"P M D'Agostino, S K Chattopadhyay","doi":"","DOIUrl":"","url":null,"abstract":"Reverse micelles were employed to test the accuracy of the widely accepted mechanism for alpha-chymotrypsin in a highly structured aqueous system similar to intracellular conditions. Results yielded from spectrophotometrical assays of the alpha-chymotrypsin catalyzed hydrolysis of both p-nitrophenyl acetate (p-NPA) and p-nitrophenyl trimethylacetate (p-NPTA) were kinetically analyzed to determine constants typical of the proposed mechanistic model. This was accomplished through the establishment of a control, i.e. the well studied buffer system, for comparison between the reverse micellular environment and a bulk aqueous solution. Control group results yielded kinetic constants in favor of the proposed mechanism (Km = 1.55 x 10(-5) +/- 1.40 x 10(-6) M for p-NPA and a Km = 4.97 x 10(-6) +/- 2.29 x 10(-7) M, Km(app) = 4.92 x 10(-6) +/- 2.33 x 10(-8) M, k2 = 4.34 x 10(-3) +/- 1.31 x 10(-3), k(cat) = 1.96 x 10(-3) +/- 2.47 x 10(-4), and Ks = 1.60 x 10(-5) +/- 4.61 x 10(-6) M for p-NPTA). In contrast, similar reactions of the enzyme in a reverse micellular system produced kinetic constants atypical to that representative of the textbook mechanism. (Km = 1.59 x 10(-4) +/- 2.70 x 10(-5) M, Ks = -8.67 x 10(-5) +/- 4.46 x 10(-5) M and Km(app) = -4.80 x 10(-5) +/- 7.05 x 10(-5) M for p-NPA and Km = 1.95 x 10(-4) +/- 9.28 x 10(-5) M, Km(app) = -1.79 x 10(-4) +/- 2.36 x 10(-5) M, and Ks = -3.95 x 10(-4) +/- 1.18 x 10(-4) M for p-NPTA). In addition to negative kinetic constants, alpha-chymotrypsin seemed to display characteristics indicative of super-activity and a hysteretic response. Overall, the widely accepted mechanism for alpha-chymotrypsin appeared to fail within the confines of reverse micelles, due to the direct influence of the system's highly structured form.","PeriodicalId":9552,"journal":{"name":"Cancer biochemistry biophysics","volume":"16 4","pages":"387-407"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20829953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Profiles of the fatty acids in the plasma membrane of human brain tumors. 人脑肿瘤质膜脂肪酸谱。

Cancer biochemistry biophysics Pub Date : 1998-11-01

E Kökoğlu, Y Tüter, Z Yazici, K S Sandikci, H Sönmez, E Z Ulakoğlu, E Ozyurt

{"title":"Profiles of the fatty acids in the plasma membrane of human brain tumors.","authors":"E Kökoğlu, Y Tüter, Z Yazici, K S Sandikci, H Sönmez, E Z Ulakoğlu, E Ozyurt","doi":"","DOIUrl":"","url":null,"abstract":"Several studies demonstrated that certain fatty acids have specific effects on tumor cells. n-3 series fatty acids (alpha-linolenic acid, eicosapentaenoic acid and docosahexaenoic acid) may suppress the carcinogenesis, whereas n-6 series fatty acids (arachidonic acid, linoleic acid) may exert tumor promoting effects. In this study, 19 patients with various brain tumors and 12 control brain tissues were studied. n-3, n-6, n-9 unsaturated fatty acids and certain saturated fatty acids levels were measured in the plasma membrane of tumor or control brain tissues by capillary gas chromatography. We found that the level of docosahexaenoic acid from n-3 series fatty acids was significantly lower in gliomas and meningiomas than controls (p = 0.000). Total n-3 fatty acids level was also significantly lower in tumors than controls (p = 0.000). The levels of linoleic acid, arachidonic acid and dihomogamma linolenic acid from n-6 series were significantly higher in gliomas and meningiomas compared with controls (p = 0.000). Total n-6 fatty acids level was also significantly higher in tumors than controls (p = 0.000). Furthermore, in total n-9 fatty acids, total unsaturated fatty acids and total saturated fatty acids levels, there were no significant differences in gliomas and meningiomas compared with controls (p = 0.6840, p = 0.4388 and p = 0.4343, respectively). This findings suggest that n-6 fatty acids can act as a tumor-promoting agent in human brain tumors.","PeriodicalId":9552,"journal":{"name":"Cancer biochemistry biophysics","volume":"16 4","pages":"301-12"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20831292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of cytoskeletal inhibitors on the accumulation of vincristine in a resistant human lung cancer cell line with high level of polymerized tubulin. 细胞骨架抑制剂对高水平聚合微管蛋白的耐药人肺癌细胞系长春新碱积累的影响。

Cancer biochemistry biophysics Pub Date : 1998-11-01

M W Chan, C D Chiang, E J Song, V C Yang

引用次数: 0

Breast cancer and benign breast disease patients evaluated in relation to oxidative stress. 评估乳腺癌和良性乳腺疾病患者与氧化应激的关系

Cancer biochemistry biophysics Pub Date : 1998-11-01

A Seven, Y Erbil, R Seven, F Inci, T Gülyaşar, B Barutçu, G Candan

引用次数: 0