Canadian journal of veterinary research = Revue canadienne de recherche veterinaire最新文献_第8页

Development of optimized protocol for culturing African swine fever virus field isolates in MA104 cells. MA104细胞培养非洲猪瘟病毒分离株优化方案的建立。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-08-09 DOI: 10.21203/rs.3.rs-754616/v1

Hyeok-Il Kwon, D. Do, H. V. Vo, Seung-Chul Lee, Min‐ho Kim, Dung Thi Thuy Nguyen, T. M. Tran, Q. T. Le, Tram-Thi Bich Ngo, N. M. Nguyen, Joo Young Lee, T. T. Nguyen

{"title":"Development of optimized protocol for culturing African swine fever virus field isolates in MA104 cells.","authors":"Hyeok-Il Kwon, D. Do, H. V. Vo, Seung-Chul Lee, Min‐ho Kim, Dung Thi Thuy Nguyen, T. M. Tran, Q. T. Le, Tram-Thi Bich Ngo, N. M. Nguyen, Joo Young Lee, T. T. Nguyen","doi":"10.21203/rs.3.rs-754616/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-754616/v1","url":null,"abstract":"The goal of this study was to identify a candidate commercial cell line for the replication of African swine fever virus (ASFV) by comparing several available cell lines with various medium factors. In the sensitivity test of cells, MA104 and MARC-145 had strong potential for ASFV replication. Next, MA104 cells were used to compare the adaptation of ASFV obtained from tissue homogenates and blood samples in various infectious media. At the 10th passage, the ASFV obtained from the blood sample had a significantly higher viral load than that obtained from the tissue sample (P = 0.000), exhibiting a mean cycle threshold (Ct) value = 20.39 ± 1.99 compared with 25.36 ± 2.11. For blood samples, ASFV grew on infectious medium B more robustly than on infectious medium A (P = 0.006), corresponding to a Ct value = 19.58 ± 2.10 versus 21.20 ± 1.47. African swine fever virus originating from blood specimens continued to multiply gradually and peaked in the 15th passage, exhibiting a Ct value = 14.36 ± 0.22 in infectious medium B and a Ct value = 15.42 ± 0.14 in infectious medium A. When ASFV was cultured from tissue homogenates, however, there was no difference (P = 0.062) in ASFV growth between infectious media A and B. A model was developed to enhance ASFV replication through adaptation to MA104 cells. The lack of mutation at the genetic segments encoding p72, p54, p30, and the central hypervariable region (CVR) in serial culture passages is important in increasing the probability of maintaining immunogenicity when developing a vaccine candidate.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"86 4 1","pages":"261-268"},"PeriodicalIF":1.1,"publicationDate":"2021-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49047987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Dissemination and characteristics of high-level erythromycin-resistant Enterococcus faecalis from bulk tank milk of dairy companies in Korea. 韩国乳业公司散装罐奶中高水平耐红霉素粪肠球菌的传播及特点。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-07-14 DOI: 10.21203/RS.3.RS-598748/V1

Yu Jin Lee, Koeun Kim, Y. J. Lee

{"title":"Dissemination and characteristics of high-level erythromycin-resistant Enterococcus faecalis from bulk tank milk of dairy companies in Korea.","authors":"Yu Jin Lee, Koeun Kim, Y. J. Lee","doi":"10.21203/RS.3.RS-598748/V1","DOIUrl":"https://doi.org/10.21203/RS.3.RS-598748/V1","url":null,"abstract":"Enterococci are environmental pathogens that can cause bovine mastitis, which is treated with macrolides, one of which is erythromycin (ERY). The aim of this study was to compare the characteristics of high-level erythromycin-resistant (HLER) Enterococcus faecalis (E. faecalis) isolates from bulk tank milk of 4 dairy companies, identified as A to D, in order to assess the threat to public health. Although isolates from company D showed the highest prevalence of E. faecalis, the prevalence of HLER E. faecalis in isolates from company A showed a significant difference. A total of 149 of the 301 HLER E. faecalis isolates showed the highest rate of resistance to tetracycline. In the distribution of antimicrobial resistance genes, 147 isolates carried the ermB gene alone and 2 isolates carried both ermA and ermB genes. Also, 72 and 60 isolates carried both tetM and tetL genes and the tetM gene alone, respectively, and 38 isolates carried the optrA gene. The prevalence of both aac(6')Ie-aph(2″)-la and ant(6')-Ia genes was the highest and 104 isolates harbored the Int-Tn gene carrying the Tn916/1545-like transposon. Although the distribution of the e rmB gene showed no significant difference among dairy companies, the prevalence of other resistance genes and transposons showed significant differences among dairy companies. Virulence genes were highly conserved in the HLER E. faecalis isolates. Our results indicated that there were significant differences in phenotypic and genotypic characteristics of HLER E. faecalis isolates in milk from 4 different dairy companies. A structured management protocol by companies and constant monitoring are therefore necessary to minimize public health hazards.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"87 1 1","pages":"51-58"},"PeriodicalIF":1.1,"publicationDate":"2021-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42373081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The accuracy of tactile assessment of canine nose temperature to identify rectal hyperthermia and hypothermia in dogs presenting on an emergency basis. 犬鼻温度触觉评估的准确性，以确定在紧急情况下狗的直肠高热和低温。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-07-01

Christopher R Kennedy, Jonathan M Babyak, Elizabeth A Rozanski

{"title":"The accuracy of tactile assessment of canine nose temperature to identify rectal hyperthermia and hypothermia in dogs presenting on an emergency basis.","authors":"Christopher R Kennedy, Jonathan M Babyak, Elizabeth A Rozanski","doi":"","DOIUrl":"","url":null,"abstract":"The goals of this study were to evaluate whether touch can identify a warm nose as opposed to a cold nose, to examine the correlation between thermographically measured nose temperatures and rectal temperatures, and to calculate the accuracy of tactile assessment of nose temperature in detecting rectal hyperthermia and hypothermia in dogs. A total of 100 dogs presenting to an emergency room was prospectively enrolled. Tactile nose assessment was carried out on triage. Noses were subjectively categorized as warm, cold, or intermediate (neither warm nor cold). Thermographic nose temperatures were recorded using a thermal imaging camera. Tactile assessment categorized noses as warm, intermediate, or cold (P < 0.01). There was no correlation between thermographically measured nose temperature and rectal temperature (r = 0.02). Tactile assessment of noses as warm had a sensitivity of 29.4% and a specificity of 79.5% for detecting rectal hyperthermia; calculated test accuracy was 71%. Tactile assessment of noses as cold had a sensitivity of 54.5% and a specificity of 62.9%; calculated test accuracy was 62%. It was concluded that nose temperatures do not correlate with rectal temperatures. Tactile assessment of nose temperature is inaccurate for identifying rectal hyperthermia or hypothermia.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 3","pages":"205-209"},"PeriodicalIF":1.1,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243800/pdf/cjvr_03_205.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39173970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of anesthesia duration on the quality of recovery in horses undergoing elective and emergency surgeries using the same anesthetic protocol. 麻醉时间对使用相同麻醉方案进行选择性和紧急手术的马的恢复质量的影响。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-07-01

Hanna Vermedal, Alexander Valverde, William Sears

{"title":"Effect of anesthesia duration on the quality of recovery in horses undergoing elective and emergency surgeries using the same anesthetic protocol.","authors":"Hanna Vermedal, Alexander Valverde, William Sears","doi":"","DOIUrl":"","url":null,"abstract":"The objective of this study was to investigate the effect of anesthesia duration on the quality of recovery in horses. The medical records of horses that were anesthetized and underwent surgery for elective and emergency soft tissue and orthopedic conditions from 2013 to 2019 were reviewed. Horses included in the study (N = 305) fulfilled the following requirements: all had the same premedication/induction protocol and the same balanced anesthesia for maintenance and were anesthetized by the same, experienced Board-certified anesthesiologist. A standardized anesthetic recovery score was completed for all horses to evaluate their recovery and the following interactions were assessed: age, body weight, breed, sex, American Society of Anesthesiologists status, type of surgical procedure, occurrence of hypotension, use of dobutamine, number of additional doses of xylazine/ketamine after isoflurane discontinuation, anesthesia duration, post-anesthetic sedation, and end-tidal isoflurane concentration during maintenance and at the time of transfer to the recovery room. These interactions were assessed based on the quality of recovery score using logistic regression. Duration of anesthesia (P = 0.021) and age (P = 0.003) negatively affected the quality of recovery. The odds of a worse recovery score were increased by 1.20-fold (1.03, 1.41; lower and upper limits) for every additional 30 min of anesthesia duration, while the odds of a worse recovery score were increased by 1.09-fold (1.03, 1.16) for every additional 1 y of age. In conclusion, the results of this retrospective study indicate that increasing the anesthesia duration negatively affects the quality of recovery in horses undergoing routine and emergency surgical procedures.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 3","pages":"193-200"},"PeriodicalIF":1.1,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243802/pdf/cjvr_03_193.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39173968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical N-palmitoyl serinol, a commensal bacterial metabolite, prevents the development of epidermal permeability barrier dysfunction in a murine model of atopic dermatitis-like skin. 外用n -棕榈酰丝氨酸醇，一种共生细菌代谢物，可防止特应性皮炎样皮肤小鼠模型中表皮渗透性屏障功能障碍的发展。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-07-01

Si Wen, Li Ye, Dan Liu, Bin Yang, Mao-Qiang Man

引用次数: 0

Whole genome sequencing of methicillin-resistant and methicillin-sensitive Staphylococcus aureus isolated from 4 horses in a veterinary teaching hospital and its ambulatory service. 某兽医教学医院及其门诊4匹马耐甲氧西林和敏感金黄色葡萄球菌的全基因组测序。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-07-01

Paloma Morice, Marion Allano, Chantale Provost, Julie-Hélène Fairbrother, Carl A Gagnon, Frédéric Sauvé

{"title":"Whole genome sequencing of methicillin-resistant and methicillin-sensitive Staphylococcus aureus isolated from 4 horses in a veterinary teaching hospital and its ambulatory service.","authors":"Paloma Morice, Marion Allano, Chantale Provost, Julie-Hélène Fairbrother, Carl A Gagnon, Frédéric Sauvé","doi":"","DOIUrl":"","url":null,"abstract":"Genomic characterization was conducted on 2 methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from 2 horses hospitalized during an overlapping period of time and 2 methicillin-sensitive S. aureus (MSSA) strains isolated from 2 distinct horses. Phylogenetic proximity was traced and the genotypic and phenotypic characteristics of the antimicrobial resistance of the strains were compared. Whole genome sequencing of MRSA strains for this report was similar but differed from whole genome sequencing of MSSA strains. The MRSA strains were closely related, belonging to sequence type (ST) 612, spa type t1257, and SCCmec type IVd2B. The MSSA strains were also closely related, belonging to ST1660, spa type t3043, and having no detectable staphylococcal cassette chromosome mec elements. All MSRA and MSSA strains were Panton-Valentine leukocidin negative. There were discrepancies in the genotypic analysis and the antimicrobial susceptibility testing (phenotypic analysis) of MRSA strains for rifampin, trimethoprim-sulfamethoxazole, gentamicin, amikacin, and enrofloxacin.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 3","pages":"218-223"},"PeriodicalIF":1.1,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243796/pdf/cjvr_03_218.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39173972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a canine lens epithelial cell line. 犬晶状体上皮细胞系的建立。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-07-01

Akira Matsuda, Yuki Shimizu, Teppei Kanda, Akihiro Ohnishi, Noritaka Maeta, Masahiro Miyabe, Kaori Saeki, Yoshiki Itoh

{"title":"Establishment of a canine lens epithelial cell line.","authors":"Akira Matsuda, Yuki Shimizu, Teppei Kanda, Akihiro Ohnishi, Noritaka Maeta, Masahiro Miyabe, Kaori Saeki, Yoshiki Itoh","doi":"","DOIUrl":"","url":null,"abstract":"Some immortalized lens epithelial cell lines have been established and are useful for molecular analysis. The establishment of additional cell lines must, however, enable a variety of in-vitro examinations. The objective of this study was to establish a new canine lens epithelial cell line by isolating CLC-1 cells from the lens tissue of a dog with cataracts. In CLC-1 cells, transforming growth factor beta (TGF-β) treatment significantly decreased gene expression of an epithelial marker and elevated that of mesenchymal markers; these characteristics are similar to those of a human lens epithelial cell line. Interestingly, CLC-1 cells exhibited lower expression of an epithelial marker and higher expression of mesenchymal markers than an anterior lens capsule. These results suggest that CLC-1 cells were derived from a cell population that was committed to epithelial-mesenchymal transition in cataract lens tissue. In conclusion, CLC-1 cells could be useful for analyzing molecular pathogenesis in canine cataracts.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 3","pages":"236-240"},"PeriodicalIF":1.1,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243803/pdf/cjvr_03_236.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39173975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-obesity effects of Celastrus orbiculatus extract containing celastrol on canine adipocytes. 含雷公藤红素的环青藤提取物对犬脂肪细胞的抗肥胖作用。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-07-01

Cho-Won Kim, Ryeo-Eun Go, Hong Kyu Lee, Byeong-Teck Kang, Woo Jae Cho, Kyung-Chul Choi

{"title":"Anti-obesity effects of Celastrus orbiculatus extract containing celastrol on canine adipocytes.","authors":"Cho-Won Kim, Ryeo-Eun Go, Hong Kyu Lee, Byeong-Teck Kang, Woo Jae Cho, Kyung-Chul Choi","doi":"","DOIUrl":"","url":null,"abstract":"From 50 to 60% of companion animals in the United States are overweight or obese and this obesity rate is rising. As obesity is associated with a number of health problems, an agent that can help weight loss in pets and assist in clinically managing obesity through veterinary prescription foods and medication would be beneficial. Many studies have shown that celastrol, a phytochemical compound found in Celastrus orbiculatus extract (COE), has anti-obesity and anti-inflammatory effects, although these effects have not yet been determined in canine or canine-derived cells. The objective of this study was to investigate the effects of celastrol on the adipogenic differentiation and lipolysis of canine adipocytes. Primary preadipocytes were isolated from the gluteal region of a beagle dog and the primary adipocytes were differentiated into mature adipocytes by adipocyte differentiation media containing isobutylmethylxanthine, dexamethasone, and insulin. In a water-soluble tetrazolium (WST) assay, the cell viability of mature adipocytes was decreased after treatment with COE (0, 0.93, 2.32, and 4.64 nM celastrol) in a concentration-dependent manner, although preadipocytes were not affected. Oil Red O (ORO) staining revealed that COE inhibited the differentiation into mature adipocytes and lipid accumulation in adipocytes. In addition, treatment with COE significantly reduced triglyceride content and increased lipolytic activities by 1.5-fold in canine adipocytes. Overall, it was concluded that COE may enhance anti-obesity activity in canine adipocytes by inhibiting lipid accumulation and increasing lipolytic activity.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 3","pages":"177-185"},"PeriodicalIF":1.1,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243799/pdf/cjvr_03_177.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39173522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beekeeping management practices in southwestern Quebec. 魁北克西南部的养蜂管理实践。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-07-01

Gabrielle Claing, Pascal Dubreuil, Julie Ferland, Martine Bernier, Julie Arsenault

{"title":"Beekeeping management practices in southwestern Quebec.","authors":"Gabrielle Claing, Pascal Dubreuil, Julie Ferland, Martine Bernier, Julie Arsenault","doi":"","DOIUrl":"","url":null,"abstract":"The growing number of honey bee colonies and beekeepers in Canada has led to a great diversity of beekeeping practices. All beekeeping operations, however, need to implement consistent management measures for the control of diseases. The objective of this study was to document the actual disease management practices of beekeeping productions in southwestern Quebec, Canada. A survey was conducted to describe management practices used by 15 beekeepers who own 1824 colonies in that area. Data were obtained by telephone interviews. When infectious diseases were suspected, beekeepers generally avoided using potentially toxic acaricides and chemical treatments associated with antimicrobial resistance and instead used preventive, physical or management methods, although laboratory diagnosis was rarely used. This study highlights the wide variety of operation sizes, activities, and disease management strategies among beekeepers in southwestern Quebec. It identifies the need to encourage the use of services available to them and to propose a standardized preventive medical approach for field veterinarians to avoid the spread of infectious diseases.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 3","pages":"229-235"},"PeriodicalIF":1.1,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243813/pdf/cjvr_03_229.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39173974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence of a novel recombinant USA/GBI29/2015-like strain of Seneca Valley virus in Guangdong Province, 2018. 一株重组USA/ gbi29 /2015样塞内卡谷病毒在广东省的出现，2018。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-07-01

Jianguo Dong, Dan Rao, Shuhai He, Fengchao Jiao, Benchi Yi, Bin Chen, Chaoliang Leng, Li Huang

引用次数: 0