Canadian journal of veterinary research = Revue canadienne de recherche veterinaire最新文献_第10页

Classification of porcine reproductive and respiratory syndrome virus in Ontario using Bayesian phylogenetics and assessment of temporal trends. 安大略省猪繁殖与呼吸综合征病毒的贝叶斯系统发育分类及时间趋势评估。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-04-01

Dylan John Melmer, Robert Friendship, Terri L O'Sullivan, Amy L Greer, Dinko Novosel, Davor Ojkić, Zvonimir Poljak

{"title":"Classification of porcine reproductive and respiratory syndrome virus in Ontario using Bayesian phylogenetics and assessment of temporal trends.","authors":"Dylan John Melmer, Robert Friendship, Terri L O'Sullivan, Amy L Greer, Dinko Novosel, Davor Ojkić, Zvonimir Poljak","doi":"","DOIUrl":"","url":null,"abstract":"Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important swine viruses globally, including in Ontario, Canada. Understanding the evolution and relation of the various PRRSV genotypes in Ontario can provide insight into the epidemiology of the virus. The objectives of this study were to i) describe the variability of PRRSV genotypes in Ontario swine herds, and ii) evaluate possible groupings based on PRRSV genomic data. Virus open reading frame 5 (ORF-5) sequences collected from 2010 to 2018 were obtained from the Animal Health Laboratory, University of Guelph and Bayesian phylogenetic models were created from these. The PRRSV population of Ontario was then categorized into 10 distinct clades. Model comparisons indicated that the model with a constant population assumption fit the data best, which suggests that the net change in the PRRS virus variation of the entire population over the last decade was low. Nonetheless, viruses grouped into individual clades showed temporal clustering during distinct time intervals of the entire study period (P < 0.01).","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 2","pages":"83-92"},"PeriodicalIF":1.1,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995535/pdf/cjvr_02_83.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38896247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative efficacy test of 1 versus 2 doses of CIRCOQ PCV2 subunit vaccine against naturally occurring PCV2-type d in piglets with high maternally derived antibodies (MDAs) on a Vietnamese swine farm. 在越南养猪场进行了1剂和2剂CIRCOQ PCV2亚单位疫苗对具有高母源性抗体(mda)的仔猪天然发生的PCV2- d型的比较效力试验。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-04-01

Duy Tien Do, Khanh Doan Vinh Tran, Anh Tuyet Quach, David Lee, Frank Cj Chang, Carol Py Wu, Toan Nguyen Tat, Chanhee Chae

{"title":"A comparative efficacy test of 1 versus 2 doses of CIRCOQ PCV2 subunit vaccine against naturally occurring PCV2-type d in piglets with high maternally derived antibodies (MDAs) on a Vietnamese swine farm.","authors":"Duy Tien Do, Khanh Doan Vinh Tran, Anh Tuyet Quach, David Lee, Frank Cj Chang, Carol Py Wu, Toan Nguyen Tat, Chanhee Chae","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to evaluate the protective efficacy of the CIRCOQ porcine circovirus type 2 (PCV2) subunit vaccine in piglets with high maternally derived antibodies (MDAs) against disease caused by natural infection with PCV2d. A total of 130 weaned, 21-day-old healthy pigs was allocated into 3 trial groups. The signs of respiratory disorder were higher in unvaccinated pigs than in vaccinated pigs at 13 to 17 weeks old (P < 0.05), 18 to 22 weeks old (P < 0.001), and 23 to 27 weeks old (P < 0.01). The unvaccinated pigs had an early rate of dermatitis at 8 to 12 weeks old (10.0%), 13 to 17 weeks old (30.0%), 18 to 22 weeks old (46.7%), and 23 to 27 weeks old (33.3%), while there were no cases of dermatitis in vaccinated pigs. There was a significant difference (P < 0.05) in the mortality of pigs in the unvaccinated group and the 2-dosed vaccinated group. PCV2 viremia was detected in the blood and peaked at 105 days old in both unvaccinated pigs (Ct-adj = 8.40) and pigs vaccinated with 1 dose (Ct-adj = 6.37), while no detectable PCV2 virus was found in the blood of pigs vaccinated with 2 doses. At 77 and 105 days old, the PCV2 viremia load (Ct-adj) of unvaccinated pigs and those vaccinated with 1 dose was significantly higher (P < 0.05) than that of the 2-dosed vaccinated pigs. The body weight (BW), average weight gain (AWG), and average daily gain (ADG) in both groups of vaccinated pigs were significantly higher (P < 0.05) than those of unvaccinated pigs. The study vaccine was significantly efficacious in protecting vaccinated pigs against clinical symptoms, blood viral load, and mortality, as well as improving productivity, compared with unvaccinated pigs.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 2","pages":"93-100"},"PeriodicalIF":1.1,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995537/pdf/cjvr_02_93.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38903467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of meloxicam on respiratory virus titers and health outcomes when administered concurrently with a modified live respiratory vaccine in abruptly weaned beef steers. 在突然断奶的肉牛中，美洛昔康与改良呼吸活疫苗同时使用时对呼吸道病毒滴度和健康结果的影响。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-04-01

Elizabeth R Homerosky, Michael J Jelinski, Craig Dorin

{"title":"Impact of meloxicam on respiratory virus titers and health outcomes when administered concurrently with a modified live respiratory vaccine in abruptly weaned beef steers.","authors":"Elizabeth R Homerosky, Michael J Jelinski, Craig Dorin","doi":"","DOIUrl":"","url":null,"abstract":"Abruptly weaned crossbred steer calves (N = 271) were used in a randomized, blinded 2-arm clinical trial to assess the impact of a long-acting non-steroidal anti-inflammatory drug on bovine herpesvirus type 1, bovine respiratory syncytial virus, parainfluenza virus type 3, and coronavirus titers and health outcomes when administered concurrently with a modified live respiratory vaccine upon arrival at a feedlot. Treatment groups included a control (saline; n = 135) and an experimental group (injectable meloxicam; n = 136). Viral antibody titers and body weight were measured on arrival, day 7, and day 21, along with a final weight on day 45. Body weight and antibody titers for all viruses increased over time (P < 0.001); however, there were no differences by treatment group or a significant group × time interaction when evaluated using repeated measures analysis of variance. Interestingly, the use of meloxicam was associated with increased treatment risk (P < 0.05). In conclusion, the administration of meloxicam may adversely affect health; however, a decreased vaccine response is likely not a contributing factor.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 2","pages":"101-105"},"PeriodicalIF":1.1,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995533/pdf/cjvr_02_101.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38903468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a SYBR Green-based real-time quantitative polymerase chain reaction assay to detect enzootic nasal tumor virus in goats. 基于SYBR green的山羊流行性鼻肿瘤病毒实时定量聚合酶链反应方法的建立。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-04-01

Rongze He, Yulan Du, Linli Gan, Muhammad Ali Mohsin, Bao-Xiang He

{"title":"Development of a SYBR Green-based real-time quantitative polymerase chain reaction assay to detect enzootic nasal tumor virus in goats.","authors":"Rongze He, Yulan Du, Linli Gan, Muhammad Ali Mohsin, Bao-Xiang He","doi":"","DOIUrl":"","url":null,"abstract":"Enzootic nasal adenocarcinoma is a contagious respiratory disease in goats that is caused by the enzootic nasal tumor virus 2 (ENTV-2). In order to increase the number of available detection methods for ENTV-2, we developed a SYBR Green real-time polymerase chain reaction (SGrPCR) assay that targets the gag gene of ENTV-2. The low limit of detection of the assay was 3.68 × 101 copies/μL, a hundredfold more sensitive than conventional PCR. The melt curve showed a single sharp melt peak at 83°C, which indicated that there was no non-specific amplification or primer dimer formation. The intra-assay and inter-assay coefficients of variation were 1.58% and 1.82%, respectively. There was no cross-reactivity with closely related goat viruses (i.e., orf virus, peste des petits ruminants virus, goatpox virus, foot-and-mouth disease virus) and endogenous retroviruses. In conclusion, the SGrPCR assay is specific for the gag gene of ENTV-2 and provides a rapid and sensitive approach for detecting ENTV-2 in clinical samples.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 2","pages":"145-150"},"PeriodicalIF":1.1,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995532/pdf/cjvr_02_145.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38896673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A genome-wide analysis of cardiac lesions of pigs that die during transport: Is heart failure of in-transit-loss pigs associated with a heritable cardiomyopathy? 在运输过程中死亡的猪心脏病变的全基因组分析:运输损失猪的心力衰竭与遗传性心肌病有关吗?

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-04-01

Katherine Zurbrigg, Francesca Bertolini, Muhammed Walugembe, Toni van Dreumel, David Alves, Robert Friendship, Terri L O'Sullivan, Max F Rothschild

{"title":"A genome-wide analysis of cardiac lesions of pigs that die during transport: Is heart failure of in-transit-loss pigs associated with a heritable cardiomyopathy?","authors":"Katherine Zurbrigg, Francesca Bertolini, Muhammed Walugembe, Toni van Dreumel, David Alves, Robert Friendship, Terri L O'Sullivan, Max F Rothschild","doi":"","DOIUrl":"","url":null,"abstract":"While heart failure is a primary cause of death for many in-transit-loss (ITL) pigs, the underlying cause of these deaths is not known. Cardiomyopathies are considered a common cause of heart failure in humans and often have a genetic component. The objective of this study was to determine if genes associated with cardiomyopathies could be identified in ITL pigs. Samples from the hearts of pigs that died during transport to an abattoir in Ontario, Canada were collected and genotyped along with samples from pigs that did not die during transport (ILT hearts: n = 149; non-ITL/control hearts: n = 387). Genome-wide analyses were carried out on each of the determined phenotypes (gross cardiac lesions) using a medium density single nucleotide polymorphism (SNP) chip and 500 kb windows/regions for analysis, with 250 kb regions of overlap. The distribution derived by a multidimensional scaling (MDS) analysis of all phenotypes demonstrated a lack of complete separation between phenotypes of affected and unaffected animals, which made diagnosis difficult. Although genetic differences were small, a few genes associated with dilated cardiomyopathy (DCM) and arrhythmogenic right ventricular cardiomyopathy (ARVM) were identified. In addition, multiple genes associated with cardiac arrhythmias and ventricular hypertrophy were identified that can possibly result in heart failure. The results of this preliminary study did not provide convincing evidence that a single, heritable cardiomyopathy is the cause of heart failure in ITL pigs.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 2","pages":"119-126"},"PeriodicalIF":1.1,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995549/pdf/cjvr_02_119.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38903471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of clinical and inflammatory parameters in dogs with pyometra before and after ovariohysterectomy. 犬卵巢子宫切除术前后子宫积脓的临床和炎症参数比较。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-01-13 DOI: 10.21203/RS.3.RS-143024/V1

Soomin Ahn, Hyun-Jung Han, Jinho Park, Sang-Ki Kim, D. Jung, Dohyeon Yu

{"title":"Comparison of clinical and inflammatory parameters in dogs with pyometra before and after ovariohysterectomy.","authors":"Soomin Ahn, Hyun-Jung Han, Jinho Park, Sang-Ki Kim, D. Jung, Dohyeon Yu","doi":"10.21203/RS.3.RS-143024/V1","DOIUrl":"https://doi.org/10.21203/RS.3.RS-143024/V1","url":null,"abstract":"This study aimed to identify potential biomarkers of canine pyometra and their correlations with clinical parameters. First, 90 dogs with pyometra and 26 healthy female dogs were compared. Then, paired samples (before and after ovariohysterectomy) from 22 dogs with pyometra and 9 healthy controls from the initial cohort were compared. Concentrations of acute inflammatory proteins, C-reactive protein (CRP) and serum amyloid A (SAA), and cell-free DNA (cfDNA), were significantly higher in dogs with pyometra than in clinically healthy dogs. Cell-free DNA was the most sensitive biomarker for systemic inflammation, based on the receiver operating characteristic curve analysis (area under the curve = 0.959). In addition, cfDNA and CRP were significantly associated with inflammation and organ injury-related clinical parameters. Following the surgical removal of the inflamed uterus, interleukin-6 (IL-6), high-mobility group box 1 (HMGB1), and procalcitonin (PCT) significantly decreased, whereas changes in CRP, SAA, and cfDNA were not significant. These findings indicate that cfDNA, CRP, and SAA are potential clinical biomarkers of systemic inflammation in dogs with pyometra and PCT, IL-6, and HMGB1 are potential biomarkers of clinical recovery.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 4 1","pages":"271-278"},"PeriodicalIF":1.1,"publicationDate":"2021-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47822796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

An initial genome-wide investigation of protein-losing enteropathy in Gordon setters: Exploratory observations. 戈登赛特人蛋白质丢失性肠病的初步全基因组调查:探索性观察。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-01-01

Elle K Donnini, Muhammed Walugembe, Max F Rothschild, Albert E Jergens, Karin Allenspach

{"title":"An initial genome-wide investigation of protein-losing enteropathy in Gordon setters: Exploratory observations.","authors":"Elle K Donnini, Muhammed Walugembe, Max F Rothschild, Albert E Jergens, Karin Allenspach","doi":"","DOIUrl":"","url":null,"abstract":"The objective of this preliminary study was to identify genomic regions that may predispose Gordon setters from the United Kingdom to familial protein-losing enteropathy (PLE) at a young age. A total of 106 related Gordon setters was used, including 6 affected dogs from an affected litter, 6 case controls from the same litter, 10 related/affected dogs, and 84 related/unaffected dogs. Genomic DNA was collected from each Gordon setter and extracted from buccal mucosal swabs. Genotyping of affected and unaffected dogs was carried out using the Canine Illumina HD SNP array and data generated were analyzed with PLINK software, using fixation index (Fst) and runs of homozygosity (ROH) methods. Pairwise Fst analyses between the affected and unaffected Gordon setter dogs identified various regions of differentiation on chromosomes 10, 18, 21, and 23 that contained several important genes. These regions revealed 5 candidate genes, including RARB, TTC7A, SOCS5, PIGF, and RHOD, that are associated with human inflammatory bowel disease (IBD) and could potentially be associated with PLE in Gordon setters. Run of homozygosity (ROH) analyses revealed additional unique regions on chromosomes 15 and 17. These regions contained genes SYT1, UCN, and FNDC that could also be potential candidates for PLE in Gordon setters. The biological functions of the identified genes provided initial insights into the pathophysiology of PLE. Further large-scale studies are warranted to investigate the possible causality of these genomic regions and any possible genetic markers that could be used in predicting susceptibility to PLE syndrome.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 1","pages":"51-60"},"PeriodicalIF":1.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747665/pdf/cjvr_01_51.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39110192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of gene expression of prostaglandin EP4 receptor in canine osteosarcoma. 前列腺素EP4受体在犬骨肉瘤中的基因表达分析。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-01-01

Margaret L Musser, Austin K Viall, Rachel L Phillips, Jesse M Hostetter, Chad M Johannes

引用次数: 0

Changes in serum protein electrophoresis profiles and acute phase proteins in calves with diarrhea. 犊牛腹泻血清蛋白电泳谱和急性期蛋白的变化。

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-01-01

Kyoung-Seong Choi, Jin-Hee Kang, Hyung-Chul Cho, Do-Hyeon Yu, Jinho Park

{"title":"Changes in serum protein electrophoresis profiles and acute phase proteins in calves with diarrhea.","authors":"Kyoung-Seong Choi, Jin-Hee Kang, Hyung-Chul Cho, Do-Hyeon Yu, Jinho Park","doi":"","DOIUrl":"","url":null,"abstract":"Calf diarrhea leads to substantial economic losses in the livestock industry worldwide due to medical treatment costs, retarded growth performance, and even death. The objective of this study was to investigate changes in serum protein profiles and acute phase proteins in calves with diarrhea and identify the association between these changes and diarrhea. A total of 185 Korean beef calves were used and divided into 3 groups by age: 1 to 10 days (n = 46), 11 to 20 days (n = 65), and 21 to 30 days (n = 74). Blood and fecal samples were collected from each calf. Serum concentrations of total protein, protein fractions (albumin, α1-globulin, α2-globulin, β-globulin, and γ-globulin), haptoglobin (Hp), and serum amyloid A (SAA) were analyzed. Compared to calves without diarrhea, calves with diarrhea had significantly lower albumin concentrations at 11 to 20 days and 21 to 30 days of age (P = 0.017 and P = 0.000, respectively) and significantly higher α1-globulin fractions at 21 to 30 days of age (P = 0.01). Interestingly, α2-globulin fractions were significantly higher in diarrheic calves in all age groups, whereas γ-globulin fractions were significantly lower in calves with diarrhea aged 1 to 10 days, compared with normal animals. In calves with diarrhea, the concentration of Hp was significantly higher, whereas SAA levels were not different between normal and diarrheic calves. In addition, a positive correlation was found between α2-globulin and Hp (P = 0.0004). Taken together, these results provide useful information about the use of serum protein profiles and Hp as prognostic and diagnostic markers for animal health status.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 1","pages":"45-50"},"PeriodicalIF":1.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747664/pdf/cjvr_01_45.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39110191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in mRNA of immune factors expressed by milk somatic cells of Holstein cows with hypocalcemia after calving. 产犊后低钙荷斯坦奶牛乳体细胞免疫因子mRNA表达的变化

IF 1.1 4区农林科学

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-01-01

Hiromichi Ohtsuka, Misa Ohsawa, Kenji Murakami, Ryo Murata, Toshihide Kato, Motoshi Tajima

{"title":"Changes in mRNA of immune factors expressed by milk somatic cells of Holstein cows with hypocalcemia after calving.","authors":"Hiromichi Ohtsuka, Misa Ohsawa, Kenji Murakami, Ryo Murata, Toshihide Kato, Motoshi Tajima","doi":"","DOIUrl":"","url":null,"abstract":"Changes in immune factors expressed by milk somatic cells from Holstein cows with hypocalcemia after calving were investigated in this study. Fourteen multiparous Holstein cows after their 3rd or 4th calving in one farm were used. The cows were divided into 2 groups: 7 cows needing treatment due to onset of hypocalcemia (hypocalcemia group; age = 5.53 ± 0.27 years, parity = 3.14 ± 0.14) and 7 cows without health problems (control group; age = 5.88 ± 0.31 years, parity = 3.57 ± 0.26). Milk samples were collected aseptically using a cannula and mRNA of immune factors expressed by milk somatic cells were analyzed. Milk samples (50 mL) were collected from the right rear mammary gland of cows before milking at day 1 and weeks 1, 2, 4, and 8 after calving. All milk samples showed a negative reaction to the California Mastitis Test. Levels of relative interleukin (IL)-6 and cathelicidin in the hypocalcemia group were lower than those in the control group in weeks 1 to 8. A significant difference in relative IL-6 levels was found in week 4 (P < 0.05). These results suggest that levels of IL-6 expressed by milk somatic cells may be affected by hypocalcemia in dairy cows.","PeriodicalId":9550,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 1","pages":"72-76"},"PeriodicalIF":1.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747659/pdf/cjvr_01_72.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39122578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0