{"title":"THE CONCENTRATION OF FIBRINOLYSIN SOLUTIONS CONTAINING GLYCEROL.","authors":"R. H. Painter, G. McVicar","doi":"10.1139/O63-256","DOIUrl":"https://doi.org/10.1139/O63-256","url":null,"abstract":"A simple technique is described for using dry Sephadex G25 to concentrate solutions of fibrinolysin dissolved in a solution containing 50% of glycerol as a stabilizer. It is shown that the percentage concentration of glycerol is not appreciably disturbed by this technique.","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"14 1","pages":"2269-72"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75384482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"AEROBIC GLYCOLYSIS OF X-IRRADIATED THYMOCYTES.","authors":"K ARAKI, D K MYERS","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2157-69"},"PeriodicalIF":0.0,"publicationDate":"1963-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23666652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE EFFECT OF ADRENALECTOMY, DIHYDROERGOTAMINE, AND DIBENZYLINE ON THE SENSITIVITY OF RATS TO TOLBUTAMIDE.","authors":"R D HAWKINS, R E HAIST","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2189-95"},"PeriodicalIF":0.0,"publicationDate":"1963-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23666655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"POTASSIUM MOVEMENTS IN RAT UTERUS STUDIED IN VITRO. I. EFFECTS OF TEMPERATURE.","authors":"E. Daniel","doi":"10.1139/O63-236","DOIUrl":"https://doi.org/10.1139/O63-236","url":null,"abstract":"1. Potassium movements have been studied in vitro in uteri of estrogenpretreated rats with 42K as a tracer. At 37 °C the uterus was nearly in potassium balance in Krebs–Ringer bicarbonate and the e...","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"65 1","pages":"2065-84"},"PeriodicalIF":0.0,"publicationDate":"1963-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88298115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"MECHANICAL PROPERTIES AND HISTAMINE AND SEROTONIN CONTENT OF GUINEA PIG LUNGS AS INFLUENCED BY MICROPARTICLES INHALATION.","authors":"E. Robillard, Y. Alarie","doi":"10.1139/Y63-244","DOIUrl":"https://doi.org/10.1139/Y63-244","url":null,"abstract":"In previous experiments, the inhalation of aluminum or iron oxide submicronic particles was shown to possess strong constricting properties, using the method of volume-pressure curves with atelectatic guinea pig lung. The constricting eHect of aluini1111m was prevented by cyproheptadine, an ailtihistainiilic and antiserotonill substance. In the present experiments, determinations of serotonin and histai-nine in dust-treated lungs were undertaken; the content of serotonin and of histanline was found to be increased after aluini~lum and after iron oxide particle inhalation respectively. Serotonin and histamine given as aerosols to guinea pigs reprodt~ced the characteristic volume-pressure curves obtained after aluminum or iron oxide n~icroparticle inhalation, respectively.","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"10 1","pages":"2177-82"},"PeriodicalIF":0.0,"publicationDate":"1963-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87627836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"MECHANICAL PROPERTIES AND HISTAMINE AND SEROTONIN CONTENT OF GUINEA PIG LUNGS AS INFLUENCED BY MICROPARTICLES INHALATION.","authors":"E ROBILLARD, Y ALARIE","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2177-82"},"PeriodicalIF":0.0,"publicationDate":"1963-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23666654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PARTICLES IN DISRUPTED RAT LIVER MITOCHONDRIA.","authors":"C V LUSENA, C M DASS","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2205-8"},"PeriodicalIF":0.0,"publicationDate":"1963-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23666657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P J LUPIEN, F A VANDENHEUVEL, W G HUNSAKER, G V HATINA
{"title":"EFFECT OF AN ANTIBIOTIC ON LIPID FRACTIONS IN PLASMS AND EGG YOLK OF LAYING HENS.","authors":"P J LUPIEN, F A VANDENHEUVEL, W G HUNSAKER, G V HATINA","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2171-5"},"PeriodicalIF":0.0,"publicationDate":"1963-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23666653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PARTIAL PURIFICATION AND PROPERTIES OF PROLYL-RNA SYNTHETASE OF RAT LIVER.","authors":"M. J. Fraser, D. Klass","doi":"10.1139/O63-239","DOIUrl":"https://doi.org/10.1139/O63-239","url":null,"abstract":"Prolyl-RNA (prolyl ribonucleic acid) synthetase has been purified 30-fold from a 105,000 × g supernatant of a rat liver homogenate by precipitation at pH 5.0, heat treatment at 55 °C for 3.0 minutes in the presence of 1.0 mM ATP (adenosine triphosphate), and by ammonium sulphate fractionation. The enzyme catalyzed proline-dependent ATP-32PP (PP, inorganic pyrophosphate) exchange and the formation of prolyl hydroxamate and of prolyl-RNA. Although the enzyme did not catalyze the formation of hydroxyprolyl-RNA, it catalyzed a slight hydroxyproline-dependent ATP-32PP exchange and the formation of a small amount of hydroxyprolyl hydroxamate which was much less than the amount of prolyl hydroxamate formed under the same conditions. The enzyme is thus not quite specific for proline activation, but is specific for amino acyl-RNA formation. It is probably concerned in protein biosynthesis.In the proline-dependent ATP-32PP exchange reaction the enzyme showed optimum activity in the pH range 6.2–8.2 and no activity ...","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"1 1","pages":"2123-40"},"PeriodicalIF":0.0,"publicationDate":"1963-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90838422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE METABOLISM OF MUCOPOLYSACCHARIDES. I. ADRENAL STEROIDS AND THE SYNTHESIS OF GLUCOSAMINE-6-PHOSPHATE.","authors":"T. Foster","doi":"10.1139/O63-240","DOIUrl":"https://doi.org/10.1139/O63-240","url":null,"abstract":"The transamidase which synthesizes glucosamine-6-phosphate was studied in liver and connective tissue of rats. Adrenalectomy was found to result in a significant reduction in enzyme activity in liver. Adrenal steroid therapy with individual corticoids did not restore the level of activity, and cortisone caused a further significant decrease.Neither adrenalectomy nor therapy with the adrenal steroids corticosterone or cortisone effected any significant change in the level of activity in connective tissue. However, in the case of three other steroids, deoxycorticosterone, hydrocortisone, or progesterone, which were tested individually, the level was significantly decreased. Treatment with Reichstein's substance S resulted in a significantly increased enzyme activity.","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"13 1","pages":"2141-6"},"PeriodicalIF":0.0,"publicationDate":"1963-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78451287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}