Canadian journal of physiology and pharmacology最新文献_第10页

Variability in agitated saline echocardiography protocols: a cross-sectional analysis of bubble study techniques in echo labs across Alberta, Canada. 搅拌盐水超声心动图方案的可变性：加拿大阿尔伯塔回声实验室气泡研究技术的横断面分析。

IF 1.8 4区医学

Canadian journal of physiology and pharmacology Pub Date : 2026-01-01 DOI: 10.1139/cjpp-2025-0326

Logesh Selvakumar, Jonathan Choy, Roman Rashkovetsky, Thomas Jeerakathil, Michael D Hill, Dilini Vethanayagam

{"title":"Variability in agitated saline echocardiography protocols: a cross-sectional analysis of bubble study techniques in echo labs across Alberta, Canada.","authors":"Logesh Selvakumar, Jonathan Choy, Roman Rashkovetsky, Thomas Jeerakathil, Michael D Hill, Dilini Vethanayagam","doi":"10.1139/cjpp-2025-0326","DOIUrl":"10.1139/cjpp-2025-0326","url":null,"abstract":"Intrapulmonary right-to-left shunts (RLSs) are a clinical concern in hereditary hemorrhagic telangiectasia (HHT), contributing to dyspnea, brain abscess, and ischemic stroke. HHT is a genetic disorder characterized by arteriovenous malformations (AVMs), with pulmonary AVMs (PAVMs) being a major source of intrapulmonary shunting. Screening for PAVMs is essential to prevent paradoxical embolization, guide management, and mitigate mortality risk. Agitated saline contrast echocardiography (\"bubble echo\") is the recommended method for detecting and grading RLS, as outlined in International HHT Guidelines. Given Western Canada's large rural population, satellite HHT clinics and affiliated echo labs have been established to improve access to care. This observational cross-sectional study evaluated the written policies governing bubble-echo protocols across Alberta's accredited echocardiography laboratories. With the help of the College of Physicians and Surgeons of Alberta (CPSA), all CPSA-accredited adult echocardiography laboratories were contacted (as of 15 July 2024) to determine whether they perform RLS assessments and to obtain available written bubble-echo protocols. Of the 62 accredited facilities identified, 12 performed bubble-echo studies. Policy review of participating labs revealed notable variations in injection techniques and shunt grading criteria. These findings suggest that considering a standardized protocol could reduce variability and allow for more reliable comparisons over time.","PeriodicalId":9520,"journal":{"name":"Canadian journal of physiology and pharmacology","volume":" ","pages":"1-7"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147670472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of endothelin-1 levels in plasma from human coronary arteries measured by enzyme linked immunosorbent assay and Olink high-throughput proteomics platform. 酶联免疫吸附法和Olink高通量蛋白质组学平台测定冠状动脉血浆内皮素-1水平的比较

IF 1.8 4区医学

Canadian journal of physiology and pharmacology Pub Date : 2026-01-01 DOI: 10.1139/cjpp-2026-0112

Majid Anwar, Rhoda E Kuc, Catherine Bullock, George R Abraham, Janet J Maguire, Stephen P Hoole, Diane Proudfoot, Anthony P Davenport

{"title":"Comparison of endothelin-1 levels in plasma from human coronary arteries measured by enzyme linked immunosorbent assay and Olink high-throughput proteomics platform.","authors":"Majid Anwar, Rhoda E Kuc, Catherine Bullock, George R Abraham, Janet J Maguire, Stephen P Hoole, Diane Proudfoot, Anthony P Davenport","doi":"10.1139/cjpp-2026-0112","DOIUrl":"10.1139/cjpp-2026-0112","url":null,"abstract":"Endothelin-1 (ET-1) antagonists are increasingly being approved for new treatments for cardiovascular disease, where elevated ET-1 levels contribute to increased vasoconstriction. Further therapeutic targets, including coronary artery disease, are under investigation. The Olink Explore 3072 Proximity Extension Assay platform enables multiplexed high-throughput measurement of ~3000 plasma proteins, from minimal (≤6 µL) sample volumes. However, it is not known if the two oligonucleotide-tagged antibodies raised against preproET-11-212, used in this Olink assay, specifically measure biologically active ET-1 or the other inactive EDN1-encoded peptides, also secreted by human endothelial cells. Paired plasma samples from 29 patients with coronary artery disease were obtained, using a specialised intra-coronary sampling catheter, designed to obtain site specific biochemical information from within coronary arteries. We compared ET-1 concentrations measured with an ET-1 specific ELISA, demonstrated to have no cross-reactivity with other EDN1-encoded peptides versus values obtained using Olink Explore platform. Olink-measured ET-1 correlated significantly with ELISA-derived ET-1 levels (r = 0.53, p = 0.003), and Olink values predicted ELISA results. Olink ET-1 concentrations also correlated with ETB receptor levels (r = 0.40, p < 0.05). These findings indicate that the Olink Explore platform can detect relative changes in biologically active ET-1, supporting its use as a biomarker tool in clinical and translational studies.","PeriodicalId":9520,"journal":{"name":"Canadian journal of physiology and pharmacology","volume":" ","pages":"1-9"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incretins for type 2 diabetes. 2型糖尿病的肠促胰岛素。

IF 1.8 4区医学

Canadian journal of physiology and pharmacology Pub Date : 2026-01-01 DOI: 10.1139/cjpp-2025-0142

Esther Min, Andrew Goyette, Elaine Young, Katherine Moon, Natalie Bellini, Diana Isaacs

{"title":"Incretins for type 2 diabetes.","authors":"Esther Min, Andrew Goyette, Elaine Young, Katherine Moon, Natalie Bellini, Diana Isaacs","doi":"10.1139/cjpp-2025-0142","DOIUrl":"10.1139/cjpp-2025-0142","url":null,"abstract":"Type 2 diabetes (T2D) is a growing global health challenge. With prevalence projected to rise significantly in the coming decades, T2D is expected to contribute to increased healthcare costs due to associated morbidity and mortality. Characterized by insulin resistance and impaired pancreatic insulin secretion, T2D could be mediated by the effects of glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP). Incretin-based therapies have, therefore, emerged as effective treatments for improving glycemic management and weight reduction in people with T2D. Since the approval of the first twice-daily GLP-1 RA in 2005, advancements have led to the development of once-daily and once-weekly injectable formulations and an oral formulation. Furthermore, the approval of a dual GLP-1/GIP RA marked a significant milestone in incretin-based therapies. These agents have demonstrated glycemic-lowering benefits and weight loss, with once-weekly agents also reducing cardiovascular risk in high-risk populations. The most common adverse effects are gastrointestinal and can be partially mitigated with slow dose titration and patient education. Ongoing research into alternative dosing regimens, combination therapies, and strategies to improve access will be important for optimizing the long-term impact of incretin-based therapies in T2D management.","PeriodicalId":9520,"journal":{"name":"Canadian journal of physiology and pharmacology","volume":" ","pages":"1-23"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148194534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The era of continuous data is now: wearables and artificial intelligence. 现在是连续数据的时代：可穿戴设备和人工智能。

IF 1.8 4区医学

Canadian journal of physiology and pharmacology Pub Date : 2026-01-01 DOI: 10.1139/cjpp-2026-0011

Anthony G Pompa, Na Le Dang, Jennifer N Avari Silva

引用次数: 0

Aerobic and/or resistance exercise in restoring metabolic dysregulation induced by chronic sleep restriction in rats. 有氧和/或阻力运动在恢复大鼠慢性睡眠限制引起的代谢失调中的作用。

IF 1.3 4区医学

Canadian journal of physiology and pharmacology Pub Date : 2025-12-01 Epub Date: 2025-10-31 DOI: 10.1139/cjpp-2025-0019

Gülhan Cansu Şen, Muhammed Ali Aydın, Ozan Öner, Selen Yıldız, Esra Akbaş, Levent Öztürk

{"title":"Aerobic and/or resistance exercise in restoring metabolic dysregulation induced by chronic sleep restriction in rats.","authors":"Gülhan Cansu Şen, Muhammed Ali Aydın, Ozan Öner, Selen Yıldız, Esra Akbaş, Levent Öztürk","doi":"10.1139/cjpp-2025-0019","DOIUrl":"10.1139/cjpp-2025-0019","url":null,"abstract":"Chronic sleep restriction (SR) disrupts blood glucose regulation, leading to glucose intolerance and insulin resistance. Regular exercises, however, are known to enhance glycemic control. This study aimed to evaluate the regulatory effects of three distinct exercise protocols on blood glucose alterations caused by chronic rapid eye movement SR. Thirty-four Sprague-Dawley rats were allocated into five groups: control (CTRL), SR, SR plus aerobic exercise (SR + ExA), SR plus resistance exercise (SR + ExR), and SR plus combined exercises (SR + ExC). Except for the control group, all rats underwent 18 h of SR daily for 8 weeks using a modified multi-platform model. Exercise protocols included 30 min of swimming and/or vertical ladder climbing (15 repetitions/day) performed 3 days per week for 8 weeks. Following the intervention, glucose and insulin tolerance tests were conducted. Chronic SR increased blood glucose levels, while aerobic and/or resistance exercises effectively reduced or prevented this elevation. Glucose tolerance was significantly improved in all exercise groups compared to the sedentary group (intraperitoneal glucose tolerance test blood glucose 120 min: SR + ExA = 95 ± 7.7, SR + ExR = 100 ± 7.3, SR + ExC = 90 ± 12.6, SR = 119 ± 14.5 mg/dL; P < 0.05). Regular exercise may mitigate adverse metabolic effects of SR.","PeriodicalId":9520,"journal":{"name":"Canadian journal of physiology and pharmacology","volume":" ","pages":"378-389"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finding a link between the TRPV4 ion channel and angiogenesis: a potential therapeutic target for vascular remodeling. 发现TRPV4离子通道与血管生成之间的联系：血管重构的潜在治疗靶点。

IF 1.3 4区医学

Canadian journal of physiology and pharmacology Pub Date : 2025-12-01 Epub Date: 2025-11-13 DOI: 10.1139/cjpp-2024-0383

Gabriel Malka, Vanessa Salucci, Andreas Bergdahl

{"title":"Finding a link between the TRPV4 ion channel and angiogenesis: a potential therapeutic target for vascular remodeling.","authors":"Gabriel Malka, Vanessa Salucci, Andreas Bergdahl","doi":"10.1139/cjpp-2024-0383","DOIUrl":"10.1139/cjpp-2024-0383","url":null,"abstract":"Angiogenesis, the formation of new blood vessels, is crucial in ischemic heart disease to improve blood supply to the heart. Meanwhile, in cancer, inhibiting angiogenesis can limit tumor growth by reducing oxygen and nutrients. Calcium ions, key in cellular functions like proliferation and migration, play an important role in this process. Transient Receptor Potential Cation Channel, Vanilloid Subfamily Member 4 (TRPV4), a calcium-permeable channel, is highly expressed in endothelial cells lining blood vessels. This study explored the connection between TRPV4 and angiogenesis using an aortic ring assay. Aortic rings from 3-day-old C57Bl/6 pups were exposed to TRPV4 agonist (GSK1016790) and antagonist (HC067047) and standard growth media (control) after which maximal length and number of new sprouts were measured. The study found that the antagonist significantly reduced the number and length of new micro vessels, while the agonist increased sprout length. These findings highlight TRPV4's role in vascular remodeling, suggesting it could be a therapeutic target for treating diseases related to impaired blood flow and abnormal angiogenesis.","PeriodicalId":9520,"journal":{"name":"Canadian journal of physiology and pharmacology","volume":" ","pages":"409-417"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulin modulates MMP-2 and 9 activities through the control of oxidative stress in the heart of diabetic rats. 胰岛素通过控制氧化应激调节糖尿病大鼠心脏中MMP-2和9的活性

IF 1.3 4区医学

Canadian journal of physiology and pharmacology Pub Date : 2025-12-01 Epub Date: 2025-11-12 DOI: 10.1139/cjpp-2025-0037

Bruno Teixeira Martins, Isabela Jesus de Deus, Vitor Valadares Gutierres, Marcus Vinicius Souza, Thayane Christine Alves da Silva, Miliane Martins de Andrade Fagundes, Aline Coelho das Mercês, Wanderson Geraldo Lima, Carla Speroni Ceron, Sílvia Paula-Gomes

{"title":"Insulin modulates MMP-2 and 9 activities through the control of oxidative stress in the heart of diabetic rats.","authors":"Bruno Teixeira Martins, Isabela Jesus de Deus, Vitor Valadares Gutierres, Marcus Vinicius Souza, Thayane Christine Alves da Silva, Miliane Martins de Andrade Fagundes, Aline Coelho das Mercês, Wanderson Geraldo Lima, Carla Speroni Ceron, Sílvia Paula-Gomes","doi":"10.1139/cjpp-2025-0037","DOIUrl":"10.1139/cjpp-2025-0037","url":null,"abstract":"This study aimed to evaluate the effects of insulin therapy on oxidative stress markers, matrix metalloproteinases (MMPs) 2 and 9 activities, and cardiac remodeling in alloxan-induced diabetic rats. Forty-two male Wistar rats were randomly allocated into three groups (n ≤ 14 animals), for 30 days: control (CON; saline, IP); diabetes mellitus (DM; alloxan 60 mg/kg, IP); and insulin-treated diabetic (DINS; NPH insulin 4 U, twice daily, IP). After the treatment, the animals were anesthetized and euthanized, and plasma and heart were collected for biochemical, histological, and enzymatic analysis. Compared to DM, the DINS exhibited improved body and heart masses, as well as reduced food intake. Insulin significantly decreased glycemia, triglycerides, and total cholesterol. The antioxidant defenses were enhanced (increased catalase activity and concentrations of reduced glutathione), while the oxidative damage was reduced (decreased protein carbonyls and thiobarbituric acid reactive substance concentrations). Histological analysis revealed a reduction in the inflammatory nuclei and collagen deposition. MMP-2 and MMP-9 activities were lower in the DINS compared to the DM. The insulin treatment attenuated oxidative stress, modulated MMP activity, and collagen accumulation in the heart of diabetic rats. These findings support the potential role of insulin in mitigating diabetes-induced cardiac remodeling through redox balance.","PeriodicalId":9520,"journal":{"name":"Canadian journal of physiology and pharmacology","volume":" ","pages":"400-408"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-sensitivity troponin I-guided cardiovascular risk assessment in a general asymptomatic population: a cost-effectiveness analysis in 4000 adults. 高灵敏度肌钙蛋白I指导一般无症状人群心血管风险评估：4000名成人的成本-效果分析

IF 1.3 4区医学

Canadian journal of physiology and pharmacology Pub Date : 2025-12-01 Epub Date: 2025-11-18 DOI: 10.1139/cjpp-2025-0209

Goran Krstačić, Antonija Krstačić, Tin Krstačić

{"title":"High-sensitivity troponin I-guided cardiovascular risk assessment in a general asymptomatic population: a cost-effectiveness analysis in 4000 adults.","authors":"Goran Krstačić, Antonija Krstačić, Tin Krstačić","doi":"10.1139/cjpp-2025-0209","DOIUrl":"10.1139/cjpp-2025-0209","url":null,"abstract":"High-sensitivity cardiac troponin I (hs-TnI) is a promising biomarker for cardiovascular disease (CVD) risk stratification. This study assessed clinical outcomes and cost-effectiveness of an hs-TnI-guided CVD screening strategy in a general asymptomatic adult population. In a modeled observational program, 4000 adults aged 40-70 years without known CVD underwent hs-TnI testing. Participants were stratified into low (<4 ng/L), moderate (4-10 ng/L), and high (>10 ng/L) risk categories, and underwent further cardiac evaluation, and intervention, if indicated. A discrete-event microsimulation estimated CVD events, mortality, quality-adjusted life years (QALYs), and costs over 10 years. Among 4000 participants (mean age 57.1 ± 8.6 years; 52% women), 3548 (88.7%) were low-risk, 390 (9.8%) moderate-risk, and 62 (1.5%) high-risk. Noninvasive cardiac workup was performed in 452 (11.3%), and coronary angiography in 112 (2.8%). Significant coronary artery disease (CAD) was diagnosed in 49 (1.2%), with revascularization in 45. Compared to standard care, hs-TnI screening reduced CVD events by 37% and cardiovascular deaths by 34%, gaining 16.3 QALYs per 1000 participants. Incremental cost per person was €528, with an incremental cost-effectiveness ratio of €32 100/QALY, remaining cost effective in 93% of simulations. hs-TnI-guided cardiovascular risk assessment effectively stratifies asymptomatic adults, identifies subclinical CAD, and facilitates preventive intervention, appearing cost effective in reducing CVD burden.","PeriodicalId":9520,"journal":{"name":"Canadian journal of physiology and pharmacology","volume":" ","pages":"436-443"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145291232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Deciphering MMP9's dual role in regulating SOD3 through protein-protein interactions. 更正：解读MMP9在通过蛋白质相互作用调节SOD3中的双重作用。

IF 1.3 4区医学

Canadian journal of physiology and pharmacology Pub Date : 2025-12-01 Epub Date: 2025-11-12 DOI: 10.1139/cjpp-2025-0306

Flobater I Gawargi, Paras K Mishra

引用次数: 0

Lysine acetylation of aquaporin-3 does not improve lithium-induced nephrogenic diabetes insipidus. 水通道蛋白-3赖氨酸乙酰化不能改善锂致肾源性尿崩症。

IF 1.3 4区医学

Canadian journal of physiology and pharmacology Pub Date : 2025-12-01 Epub Date: 2025-11-10 DOI: 10.1139/cjpp-2025-0177

Nha V Huynh, Hung Nguyen, Kelly A Hyndman

{"title":"Lysine acetylation of aquaporin-3 does not improve lithium-induced nephrogenic diabetes insipidus.","authors":"Nha V Huynh, Hung Nguyen, Kelly A Hyndman","doi":"10.1139/cjpp-2025-0177","DOIUrl":"10.1139/cjpp-2025-0177","url":null,"abstract":"Aquaporin-3 (AQP3) is expressed in the basolateral membrane of the renal principal cell, contributing to vasopressin-mediated water reabsorption and urine concentration. We reported that post-translational acetylation of lysine 282 of AQP3 promotes water permeability. In this study, we hypothesized that AQP3 acetylation may improve polyuria in a mouse model of lithium-induced nephrogenic diabetes insipidus (Li-NDI). Wild type, AQP3 acetylation (K282Q), and deacetylation (K282R) mimetic mice were fed a lithium-containing diet or a control diet for 14 days. Body masses and spot urines were collected overtime, while urine flow and osmolality, plasma osmolality, and kidneys were collected on day 14 of the diets. All Li-NDI mice had greater urine output and water intake compared to control fed mice, and unexpectedly, this was exacerbated in female Li-NDI AQP3-acetylation and AQP3-deacetylation mice. After 14 days of lithium diet, acetylated AQP3 was almost undetectable in the kidneys of WT mice, and AQP3 localization in acetylated and deacetylated mice was minimal. In the setting of LI-NDI, the significant loss of AQP3 was not prevented in acetylated-AQP3 mice and in female mice mutation of K282 resulted in a worsened Li-NDI phenotype, suggesting that this lysine is critical for promoting sex-specific AQP3-water permeability.","PeriodicalId":9520,"journal":{"name":"Canadian journal of physiology and pharmacology","volume":" ","pages":"390-399"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0