Cancer Genomics & Proteomics最新文献_第5页

Melatonin Inhibits Chemical Carcinogen-mediated Malignant Transformation of Urothelial Cells: In Vitro Evidence. 褪黑激素抑制化学致癌物介导的泌尿道细胞恶性转化：体外证据

IF 2.6 4区医学

Cancer Genomics & Proteomics Pub Date : 2024-07-01 DOI: 10.21873/cgp.20456

Yujiro Nagata, Nguyen Thu Quynh, Hisami Aono, Kenichi Harada, Hiroshi Miyamoto, Naohiro Fujimoto

{"title":"Melatonin Inhibits Chemical Carcinogen-mediated Malignant Transformation of Urothelial Cells: In Vitro Evidence.","authors":"Yujiro Nagata, Nguyen Thu Quynh, Hisami Aono, Kenichi Harada, Hiroshi Miyamoto, Naohiro Fujimoto","doi":"10.21873/cgp.20456","DOIUrl":"10.21873/cgp.20456","url":null,"abstract":"Background/aim: The efficacy of melatonin and its biological significance in human bladder cancer remain poorly understood. This study aimed to investigate the functional role of melatonin in urothelial carcinogenesis.Materials and methods: In human normal urothelial SVHUC cells with exposure to the chemical carcinogen 3-methylcholanthrene, we assessed the effects of melatonin on the neoplastic/malignant transformation.Results: In the in vitro system with carcinogen challenge, melatonin significantly prevented the neoplastic transformation of SV-HUC-1 cells. In addition, melatonin treatment resulted in increased expression of SIRT1, Rb1, and E-cadherin, and decreased expression of N-cadherin and FGFR3 in SV-HUC-1 cells. Furthermore, publicly available datasets from GSE3167 revealed that the expression of melatonin receptor 1 and melatonin receptor 2 was significantly down-regulated in bladder urothelial carcinoma tissues, compared with adjacent normal urothelial tissues.Conclusion: These findings indicate that melatonin serves as a suppressor for urothelial tumorigenesis. To the best of our knowledge, this is the first preclinical study demonstrating the impact of melatonin on the development of urothelial cancer.","PeriodicalId":9516,"journal":{"name":"Cancer Genomics & Proteomics","volume":"21 4","pages":"388-394"},"PeriodicalIF":2.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11215424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141466341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Restoration of ARID1A Protein in ARID1A-deficient Clear Cell Carcinoma of the Ovary Attenuates Reactivity to Cytotoxic T Lymphocytes. 在 ARID1A 基因缺陷的卵巢透明细胞癌中恢复 ARID1A 蛋白可减轻对细胞毒性 T 淋巴细胞的反应。

IF 2.6 4区医学

Cancer Genomics & Proteomics Pub Date : 2024-07-01 DOI: 10.21873/cgp.20460

Risa Tsunematsu, Aiko Murai, Yuka Mizue, Terufumi Kubo, Tasuku Mariya, Rena Morita, Kenji Murata, Takayuki Kanaseki, Tomohide Tsukahara, Yoshihiko Hirohashi, Tsuyoshi Saito, Toshihiko Torigoe

{"title":"Restoration of ARID1A Protein in ARID1A-deficient Clear Cell Carcinoma of the Ovary Attenuates Reactivity to Cytotoxic T Lymphocytes.","authors":"Risa Tsunematsu, Aiko Murai, Yuka Mizue, Terufumi Kubo, Tasuku Mariya, Rena Morita, Kenji Murata, Takayuki Kanaseki, Tomohide Tsukahara, Yoshihiko Hirohashi, Tsuyoshi Saito, Toshihiko Torigoe","doi":"10.21873/cgp.20460","DOIUrl":"10.21873/cgp.20460","url":null,"abstract":"Background/aim: Clear cell carcinoma is a prevalent histological type of ovarian cancer in East Asia, particularly in Japan, known for its resistance to chemotherapeutic agents and poor prognosis. ARID1A gene mutations, commonly found in ovarian clear cell carcinoma (OCCC), contribute to its pathogenesis. Recent data revealed that the ARID1A mutation is related to better outcomes of cancer immunotherapy. Thus, this study aimed to investigate the immunotherapy treatment susceptibility of OCCC bearing ARID1A mutations.Materials and methods: Expression of ARID1A was analyzed using western blotting in ovarian cancer cell lines. OCCC cell lines JHOC-9 and RMG-V were engineered to overexpress NY-ESO-1, HLA-A*02:01, and ARID1A. Sensitivity to chemotherapy and T cell receptor-transduced T (TCR-T) cells specific for NY-ESO-1 was assessed in ARID1A-restored cells compared to ARID1A-deficient wild-type cells.Results: JHOC-9 cells and RMG-V cells showed no expression of ARID1A protein. Overexpression of ARID1A in JHOC-9 and RMG-V cells did not impact sensitivity to gemcitabine. While ARID1A overexpression decreased sensitivity to cisplatin in RMG-V cells, it had no such effect in JHOC-9 cells. ARID1A overexpression reduced the reactivity of NY-ESO-1-specific TCR-T cells, as observed by the IFNγ ESLIPOT assay.Conclusion: Cancer immunotherapy is an effective approach to target ARID1A-deficient clear cell carcinoma of the ovary.","PeriodicalId":9516,"journal":{"name":"Cancer Genomics & Proteomics","volume":"21 4","pages":"414-420"},"PeriodicalIF":2.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11215429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141466343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Analysis of Metastatic Uveal Melanoma and Differences in Male and Female Patients. 转移性葡萄膜黑色素瘤的转录组分析及男女患者的差异

IF 2.6 4区医学

Cancer Genomics & Proteomics Pub Date : 2024-07-01 DOI: 10.21873/cgp.20452

Sishir Doddi, Abdul-Rizaq Hamoud, Hunter M Eby, Xiaolu Zhang, Ali Sajid Imami, Elizabeth Shedroff, Isaac Schiefer, Jose Moreno-Lopez, David Gamm, Jaroslaw Meller, Robert E McCullumsmith

{"title":"Transcriptomic Analysis of Metastatic Uveal Melanoma and Differences in Male and Female Patients.","authors":"Sishir Doddi, Abdul-Rizaq Hamoud, Hunter M Eby, Xiaolu Zhang, Ali Sajid Imami, Elizabeth Shedroff, Isaac Schiefer, Jose Moreno-Lopez, David Gamm, Jaroslaw Meller, Robert E McCullumsmith","doi":"10.21873/cgp.20452","DOIUrl":"10.21873/cgp.20452","url":null,"abstract":"Background/aim: Uveal melanoma is an ocular malignancy whose prognosis severely worsens following metastasis. In order to improve the understanding of molecular physiology of metastatic uveal melanoma, we identified genes and pathways implicated in metastatic vs non-metastatic uveal melanoma.Patients and methods: A previously published dataset from Gene Expression Omnibus (GEO) was used to identify differentially expressed genes between metastatic and non-metastatic samples as well as to conduct pathway and perturbagen analyses using Gene Set Enrichment Analysis (GSEA), EnrichR, and iLINCS.Results: In male metastatic uveal melanoma samples, the gene LOC401052 is significantly down-regulated and FHDC1 is significantly up-regulated compared to non-metastatic male samples. In female samples, no significant differently expressed genes were found. Additionally, we identified many significant up-regulated immune response pathways in male metastatic uveal melanoma, including \"T cell activation in immune response\". In contrast, many top up-regulated female pathways involve iron metabolism, including \"heme biosynthetic process\". iLINCS perturbagen analysis identified that both male and female samples have similar discordant activity with growth factor receptors, but only female samples have discordant activity with progesterone receptor agonists.Conclusion: Our results from analyzing genes, pathways, and perturbagens demonstrate differences in metastatic processes between sexes.","PeriodicalId":9516,"journal":{"name":"Cancer Genomics & Proteomics","volume":"21 4","pages":"350-360"},"PeriodicalIF":2.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11215432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141466344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Prospero Homeobox-1 (PROX-1) οn the Oncogenic Phenotypes of Hepatocellular Carcinoma Cells. Prospero Homeobox-1 (PROX-1) 对肝细胞癌细胞致癌表型的影响

IF 2.5 4区医学

Cancer Genomics & Proteomics Pub Date : 2024-04-27 DOI: 10.21873/cgp.20448

Ji-Yun Hong, Sun-Young Park, Young-Lan Park, Ga-Ram You, Jae Hyun Yoon, Young-Eun Joo, Sung Kyu Choi, Sung-Bum Cho