Canadian journal of biochemistry最新文献_第3页

Partial purification of a ribonucleic acid cAMP-independent protein kinase from embryonic chicken muscle. 从鸡胚肌中部分纯化一种核糖核酸camp非依赖性蛋白激酶。

Canadian journal of biochemistry Pub Date : 1982-09-01 DOI: 10.1139/o82-114

A P Hudson, J Bag, B H Sells

引用次数: 0

Biosynthesis of microsomal phospholipids and mitochondrial polyglycerophosphatides in rapidly sedimenting endoplasmic reticulum. 快速沉积内质网中微粒体磷脂和线粒体聚甘油磷脂的生物合成。

Canadian journal of biochemistry Pub Date : 1982-09-01 DOI: 10.1139/o82-112

L Stuhne-Sekalec, N Z Stanacev

{"title":"Biosynthesis of microsomal phospholipids and mitochondrial polyglycerophosphatides in rapidly sedimenting endoplasmic reticulum.","authors":"L Stuhne-Sekalec, N Z Stanacev","doi":"10.1139/o82-112","DOIUrl":"https://doi.org/10.1139/o82-112","url":null,"abstract":"Rapidly sedimenting endoplasmic reticulum (RSER), which is known to be a complex between endoplasmic reticulum and mitochondria, was isolated from rat liver and purified through a sucrose density gradient by centrifugation according to a well established procedure previously published by G. C. Shore and J. R. Tata. This complex was characterized by microsomal (NADPH-cytochrome c reductase) and mitochondrial (succinate-cytochrome c reductase and NADP-isocitrate dehydrogenase) marker enzymes and was examined for the ability to synthesize microsomal lipids and mitochondrial polyglycerophosphatides. Results of these experiments showed that the RSER is capable of synthesizing key microsomal lipids, i.e., phosphatidic acid, phosphatidylcholine, and neutral lipids, as well as mitochondrial phosphatidylglycerosphate and phosphatidic acid, phosphatidylcholine, and neutral lipids, as well as mitochondrial phosphatidylglycerophosphate and phosphatidylglycerol. Furthermore, the level of synthesis of these lipids paralleled the level of activity of microsomal and mitochondrial marker enzymes found in the RSER preparation. Details of these experimental findings and some implications are discussed in view of the possible functional role of RSER.","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":"60 9","pages":"877-81"},"PeriodicalIF":0.0,"publicationDate":"1982-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18186839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Adenylate cyclase activity in brown adipose tissue of the genetically obese (ob/ob) mouse. 遗传性肥胖(ob/ob)小鼠棕色脂肪组织中腺苷酸环化酶活性。

Canadian journal of biochemistry Pub Date : 1982-09-01 DOI: 10.1139/o82-117

N Bégin-Heick, H M Heick

引用次数: 14

In situ enzymatic removal of orthophosphate by the nucleoside phosphorylase catalyzed phosphorolysis of nicotinamide riboside. 核苷磷酸化酶原位去除正磷酸盐催化了烟酰胺核苷的磷解。

Canadian journal of biochemistry Pub Date : 1982-09-01 DOI: 10.1139/o82-118

J W Shriver, B D Sykes

引用次数: 3

Changes in the heterogeneity of ribulosebisphosphate carboxylase-oxygenase in winter rye induced by cold hardening. 冷硬化诱导冬麦核酮糖二磷酸羧化酶-加氧酶异质性的变化。

Canadian journal of biochemistry Pub Date : 1982-09-01 DOI: 10.1139/o82-115

N P Huner, D B Hayden

引用次数: 8

Interaction of lipids with intestinal brush border membrane preparations. 脂质与肠刷状边界膜制剂的相互作用。

Canadian journal of biochemistry Pub Date : 1982-09-01 DOI: 10.1139/o82-116

P Proulx, J McNeil, I Brglez, D G Williamson

引用次数: 11

The effect of anoxia on anthracycline-induced DNA damage in the RPMI-6410 human lymphoblastoid cell line. 缺氧对蒽环类药物诱导的RPMI-6410人淋巴母细胞DNA损伤的影响

Canadian journal of biochemistry Pub Date : 1982-09-01 DOI: 10.1139/o82-111

L Brox, B Gowans, R To, A Belch

引用次数: 2

Essential sulfhydryl groups and the light-dependent transhydrogenase system of Rhodopseudomonas spheroides: localization of substrate binding sites and evidence for masked or buried sulfhydryl groups in the peripheral protein factor. 球形红假单胞菌的必需巯基和光依赖性转氢酶系统:底物结合位点的定位和外周蛋白因子中被掩盖或掩埋的巯基的证据。

Canadian journal of biochemistry Pub Date : 1982-09-01 DOI: 10.1139/o82-113

J A Orlando, T Pisani

引用次数: 1

Reorganization of unique and repetitive sequences during nuclear development in Tetrahymena thermophila. 嗜热四膜虫核发育过程中独特和重复序列的重组。

Canadian journal of biochemistry Pub Date : 1982-09-01 DOI: 10.1139/o82-107

C F Brunk, S G Tsao, C H Diamond, P S Ohashi, N N Tsao, R E Pearlman

{"title":"Reorganization of unique and repetitive sequences during nuclear development in Tetrahymena thermophila.","authors":"C F Brunk, S G Tsao, C H Diamond, P S Ohashi, N N Tsao, R E Pearlman","doi":"10.1139/o82-107","DOIUrl":"https://doi.org/10.1139/o82-107","url":null,"abstract":"Genomic libraries of macro- and micro-nuclear DNA of the ciliate protozoan Tetrahymena thermophila were constructed in the bacteriophage vector lambda gt WES lambda B. Screening of these libraries with a probe for the repeated hexamucleotide sequence C4A2 showed many phage from the micronuclear library but few if any macronuclear sequences having homology to this probe. This is consistent with C4A2-repeating elements being present predominantly if not exclusively at or near the termini of macronuclear DNA. Sequences flanking C4A2-repeating elements were isolated from a number of purified phage and were used as hybridization probes to restriction endonuclease digested macro- and micro-nuclear DNA. These experiments revealed a repeated sequence family as well as unique sequences present only in micronuclear DNA. The repeated sequence element appears to be dispersed throughout the genome. Phage-containing individual members of this micronucleus limited sequence family were purified from the micronuclear library. Some of these phage contained sequences which hybidized to macronuclear DNA. These fragments therefore contain a \"transition\" region between micronucleus-limited sequences and sequences present in both nuclei.","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":"60 9","pages":"847-53"},"PeriodicalIF":0.0,"publicationDate":"1982-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18186836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

The effect of ultraviolet radiation on early stages of activation of human lymphocytes: inhibition is independent of effects on DNA. 紫外线辐射对早期人类淋巴细胞活化的影响:抑制是独立于对DNA的影响。

Canadian journal of biochemistry Pub Date : 1982-09-01 DOI: 10.1139/o82-108

G Castellanos, T Owens, C Rudd, T Bladon, Setterfield, J G Kaplan

{"title":"The effect of ultraviolet radiation on early stages of activation of human lymphocytes: inhibition is independent of effects on DNA.","authors":"G Castellanos, T Owens, C Rudd, T Bladon, Setterfield, J G Kaplan","doi":"10.1139/o82-108","DOIUrl":"https://doi.org/10.1139/o82-108","url":null,"abstract":"Low doses (30-84 ergs/mm2, 1 erg = 10(7) J) of ultraviolet radiation (UV) caused severe inhibition of the proliferation of human lymphocytes in vitro. Greatest inhibition was produced when resting cells were irradiated immediately prior to stimulation with concanavalin A (Con A); this was true whether activation was measured by the incorporation of labelled leucine, uridine, or thymidine. If UV was applied at 44 h after culture in presence of Con A, the incorporation of [3H]thymidine measured 4 h later was seen to be inhibited but transcription and translation were scarcely affected. UV, applied after the appearance of the Con A induced thymidine transport system, did not inhibit its function. The disaggregation of chromatin and increase in nuclear volume characteristic of, and essential to, the proliferative response of lymphocytes were completely inhibited if the cells were irradiated before mitogen was added to the cultures, but were unaffected if irradiation occurred after 16 h of culture in presence of Con A. Cells irradiated with 84 ergs/mm2 at the onset of culture with mitogen did not show the early increase of cation pump function which is a characteristic of stimulated lymphocytes, when this was measured by means of 86Rb uptake after 2-4 h culture. The mitogen-stimulated activation of cation pump function has previously been shown to be unaffected by concentrations of cycloheximide and actinomycin D which produce virtually complete inhibition of protein and RNA synthesis, respectively. The major inhibitory effect of UV treatment of lymphocytes at onset of culture with Con A is therefore not on DNA or on DNA synthesis, but on some component(s) of the early activation process, possibly at the cell periphery; inactivation of this component prevents cells from proceeding into later stages of the proliferative pathway.","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":"60 9","pages":"854-60"},"PeriodicalIF":0.0,"publicationDate":"1982-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18186837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9