Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine最新文献

{"title":"Studies Regarding the Detection of Sulfonamide Residues and Evaluation of the Level of Contamination in Poultry Meat","authors":"Gabriela Valentina Vesa, Anette Iudit GHEIŢI-HEGHEDUŞ, M. Mihaiu, L. Colobatiu, Dariana Bratfelan, A. Cioca","doi":"10.15835/buasvmcn-vm:2021.0026","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0026","url":null,"abstract":"The excessive or uncontrolled use of antibiotics in poultry farming can lead to contaminated food products. Subsequently, the human consumption of products contaminated with these substances increases the global phenomenon of antibiotic resistance. The aim of this study was to develop and validate a high-performance liquid chromatography (HPLC) method suitable for the determination of sulfonamide residues in poultry meat and to check the presence of residues in random samples. The level of antimicrobial resistance was identified in order to evaluate the current stage and to estimate the tendency of this phenomenon. The HPLC method validation was performed in accordance with Commission Regulation (EC) No 657/2002 of 14 August 2002 Samples of fresh meat collected from a poultry slaughterhouse were analysed using the validated method in order to reveal the level of contamination. The data from EFSA was collected and analysed following the antimicrobial resistance for isolates of Salmonella spp., E. coli and Campylobacter spp. The method had good selectivity, linearity (R2 ≥ 0.99), precision (<6%), recoverybetween 97.7-109.6% and low limits of detection (LOD) and quantification (LOQ) Sulfaquinoxaline residues were present in 2 samples and the level of contamination did not exceed the Maximum Residue Level (31.98 ± 5.18 µg/kg and 23.70 ± 3.84 µg/kg). The analysis of data from EFSA highlighted the general presence of antimicrobial resistance especially for the following antibiotics: ciprofloxacin, tetracycline, nalidixic acid, ampicillin, sulfamethoxazole and trimethoprim. The present study brings a contribution to the process of stopping antibiotic resistance through new methods of monitorization of sulfonamide residues. The statistical data shows that there is a direct correlation between the market availability of antibiotics used in poultry farming and the occurrence of antibiotic resistance. ","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73589601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determinants of Infection as Genetic Indicators in Cows Mastitis","authors":"B. Lungu, O. Georgescu, B. Tudor, C. Mircu, P. Barrow, Hortensja Ł. Brzóska, I. Huțu","doi":"10.15835/buasvmcn-vm:2021.0036","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0036","url":null,"abstract":"The resistance to antimicrobial substances severely impacts public health and the abuse of antibiotics leads to antimicrobial resistance (AMR) or the antibiotic \"resistome\" (Wright, 2007). Bovine mastitis is largely diagnosed in dairy farms and is caused by a variety of pathogens including Streptococcus spp., Staphylococcus spp. and Escherichia coli. AMR gene expression testing in bacteria involved in mastitis in dairy cows was performed. Milk samples were subjected to the California Mastitis Test. Positive samples were transferred using eSwab, cultured on Columbia blood agar and on MacConkey agar. The Qiagen DNeasy kit was used for DNA extraction and qPCRs were run using an Agilent thermocycler. In most of the samples tested (n = 42, from three different lactating farms), the presence of ampC (36 out of 42; 85.7%) and blaZ (95.2%), correlated with confirmed resistance to beta-lactam and cephalosporin antibiotics. A variable presence of other tested AMR genes was detected, including ermB, resistance to lincosamide, macrolide (35.7%), ermC (28.6%), erythromycin resistance, mecA,methicillinresistance (42.9%), and tetK, tetracyclineresistance (78.6%). The phenomenon of antimicrobial resistance is present in dairy farms in West Romania. Multiple AMR genes were detected intested samples, with the highest resistance observed to beta-lactam antibiotics and cephalosporins.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80442698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Evaluation of the Quality Parameters of Raw Buffalo Milk Using a High-Performance Equipment","authors":"Filippos Georgios Nikolau, A. Fat, Alexandra Negrila, Arghir Ilea, R. Mihaiu, M. Mihaiu, S. Dan","doi":"10.15835/buasvmcn-vm:2021.0028","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0028","url":null,"abstract":"The aim of this study was to evaluate the quality parameters of raw buffalo milk, by determining the physicochemical parameters with major impact in terms of milk quality, number of somatic cells and total number of viable counts, at the level of a commercial farm. Over a period of 12 months, samples of raw buffalo milk were collected and analyzed from a farm authorized for the production of buffalo milk, resulting in a dynamic evaluation of quality parameters. The raw milk samples were analyzed using the - CombiFossTM7 equipment. which analyzes all the parameters necessary for official controls of milk production and quality. The averages for the results of the physicochemical parameters fat, protein, lactose, total solids and urea were 8.821 g/100 g, 4.400 g/100 g, 4.291 g/100 g, 17.93 g/100g and 27.08 mg/dL, respectively. Even if the gross composition does not exceed the allowed limits, the TVC exceeded the European standards in the case of 58.3% of the analyzed samples, the annual average being 2059.65x1000 cfu/ml. The value of the SCC average obtained was 304.85x1000 cells/ml, in accordance with the European standard. The values of SCC and TVC are increasing during the sheltering period, which means poor hygiene conditions.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86398179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Study on Radiographic Diagnosis of Esophageal Foreign Bodies in Dogs","authors":"Vlad Lupu, N. Tudor","doi":"10.15835/buasvmcn-vm:2021.0020","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0020","url":null,"abstract":"Obstruction caused by esophageal foreign bodies is a common condition in dogs, usually due to dietary indiscretion. The aim of the study was the clinical and imaging description of a series of dogs with esophageal foreign bodies. This study included 11 dogs of various breeds, ages and both sexes that showed signs compatible with esophageal obstruction. The imaging examination revealed the presence of foreign bodies (represented by bones and metal objects) in different segments of the esophagus, including both the cervical segment (2 cases) and intrathoracic segment (9 cases). Given the consequences of the presence of foreign bodies in the esophagus, the imaging examination represent the main tool in determining the location of the foreign body for therapeutic management.  ","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83726654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Sex Identification Using Different Biological Samples from Four Companion Bird Species","authors":"M. Turcu, A. Paștiu, L. Bel, Vlad Cocostîrc, F. Lucaci, D. Pusta","doi":"10.15835/buasvmcn-vm:2021.0027","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0027","url":null,"abstract":"Most companion birds have no distinct sexual dimorphic traits. The aim of this study was to identify the CHD genes from sexual chromosomes of birds (ZW) from various tissue samples. Feathers, oral swabs, blood and various tissues (by necropsy) were collected from four companion bird species (Gallus gallus domesticus, Melopsittacus undulatus, Psittacus erithacus, Ara ararauna). Feathers were processed mechanically using the Tissue Lyser II. DNA isolation was performed using the Isolate II Genomic DNA, Bioline kit. PCR was performed according to Griffiths et al. (1998) using P2/P8 primers. Chicken whose DNA sexing was identified based on phenotypic characters, was confirmed by PCR. Budgerigars whose DNA sexing identified a male and female have been confirmed based on necropsy results. African Grey Parrot whose sex was identified by DNA sexing as female was confirmed based on a genetic sexing previously performed. The Blue and Yellow Macaw, whose sex was determined as male by DNA sexing, was confirmed based on necropsy results. DNA concentration was higher in blood samples than in oral swabs or feathers. Blood collection is considered an invasive procedure in birds. For feather samples, mechanical denaturation before DNA extraction is recommended. Oral swab samples are suitable for isolating and amplifying DNA in birds.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81112130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the Distribution of Coronary Arteries in Pig (Sus Scrofa Domesticus)","authors":"G. Zagrai, C. Belu, I. Dumitrescu, S. Raita, M. Dobrila, G. Cotor, Sorina Andreea Mihai, D. Cotor, A. Damian","doi":"10.15835/buasvmcn-vm:2021.0031","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0031","url":null,"abstract":"Pigs are some of the most widely used experimental models, an advantage being the characteristics of homeostasis and many morphological features comparable to those in humans. For this reason, we addressed this topic in this paper, with the major objective of completing the data and even identifying some features not described in the literature. The present study was performed on 20 pig carcasses, with different weights and ages (2-4 months). The aim was to highlight some peculiarities regarding the coronary artery system in this species, using as a method the injection of vessels with contrasting plastic material. The collaterals and terminals of the coronary arteries, their distribution territories as well as the identified individual variants were described. As a general conclusion, regardless of the polymorphism in all dissected cases, the left coronary artery was the dominant artery, there being no exception from the data known in the literature.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83471307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can Wheat Germination Test Be Used to Predict Pregnancy in Ewes?","authors":"N. Constantin, Andra Șipoș, Florin Petrișor Posastiuc, Crina Andrei, Ioana Cristina Sprințu","doi":"10.15835/buasvmcn-vm:2021.0035","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0035","url":null,"abstract":"Several invasive diagnosis methods to predict pregnancy in humans and animals were used in the past. Since Antiquity, grains germination test was used to predict more safely the pregnancy in women in order to avoid the possibility of miscarriage. The abscisic acid is a component of pregnant females’ urine that prevents germination of grains. Considering this principle, 20 samples of urine were collected from 10 pregnant ewes and 10 from non-pregnant animals. After urinalysis, 15 ml urine sample was added to 20 grains of wheat in a Petri dish, followed by a dilution of 1:4 with distilled water. Only water has been added in the control sample. There were performed duplicates for each animal. For 10 days, all plates were kept in a constant environmental temperature (22°C) and humidity (42%). Statistical analysis revealed that after 5 and 10 days, wheat grains belonging to pregnant ewes were less germinated, and values regarding shoot length were lower than those from the control group. Even if the number of samples is small, it can be concluded that wheat germination test can be used to predict pregnancy in ewes.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82031667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Plasma Oxidative Stress Biomarkers and Metabolic Parameters of Dairy Heifers: Relationship with Success at First Service","authors":"Sofiane Boudjellaba, Habiba Saadi, M. Zaouani, L. Ainouz, A. Hani, Hayat Remichi, D. Saidj, Takfarinas Idres, M. Iguer-ouada","doi":"10.15835/buasvmcn-vm:2021.0007","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0007","url":null,"abstract":"First insemination success is considered as good indicator of heifer fertility to ensure renewal of dairy herds. The objective of this study was to determine the relationship between first insemination success and biomarkers of oxidative stress (OS). A total of 25 heifers were divided into two groups according to their success at first insemination: group FS+ (heifers that were pregnant at first service, n = 14) and group FS- (heifers that were not pregnant at first service, n = 11). The serum of these two groups were analyzed for malondialdehyde (MDA), glutathione S-transferase (GST), reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), myeloperoxidase (MPO), nitric Oxide (NO) and superoxide dismutase (SOD) as oxidative stress biomarkers and biochemical parameters. Heifers in the group FS+ showed no significant difference in all OS parameters compared to heifers in the group FS-. The OS parameters showed almost similar values in both groups except for GST and CAT where the difference was at the limit of significance. The plasma concentration of OS biomarkers assessed in our study were not related to first service success in heifers. Further studies are needed to clarify the role of oxidative status in the reproductive performance of heifers.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86257510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trauma Etiology in Dogs and Cats: A Retrospective Study of 4626 Cases","authors":"R. Cojocaru, L. Schuszler, D. Bumb, Rebeca Barnea, C. Igna","doi":"10.15835/buasvmcn-vm:2021.0015","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0015","url":null,"abstract":"Traumatic injuries are a major health hazard having an important impact in animals’ welfare. The outcome following a traumatic event in the animal’s life, depends on multiple factors, the most common ones being the cause of trauma, the location of the trauma and the amount of kinetic energy and the way it was distributed to the animal, but also human activities and lifestyles, human-pet relationships and pet-management local strategies. This retrospective study aims to evaluate the impact of traumatic injuries in 4626 dogs and cats that presented to the Surgical Department from the USAMVBT’s Veterinary Hospital between 2000 and 2020. The most frequent traumatic injuries were represented by road traffic accidents, young animals under 1 year of age being more prone than other age groups χ2(4, N=4626) =43, p<.001 and dogs being more affected than cats χ2(1, N=4626) =107.66, p<.001.  Although non-accidental injuries had a low frequency in our study, there is a tendency of misdistribution these cases in the other categories like road traffic accidents. By reporting non-accidental injuries, it could provide a better understanding and better perspective on the real number of abused animals.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88237360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regression Models Based on Ultrasonography Fetal Ocular Measurements for Determining Gestational Age in Arab-Barb Mares","authors":"M. Mebarki, K. Benhenia","doi":"10.15835/buasvmcn-vm:2021.0008","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0008","url":null,"abstract":"The aim of the present study was to develop regression models based on ultra-sonographic measurements of fetal eyes (orbital diameter (OD) and eyes volume (EV)) to determine mare gestational age (fetal age) in Arab-Barb breed and to compare the obtained formula with similar formulas used in practice. Twenty-two Arab-Barb mares were included in this study. For each mare, an average of 6 ultrasound examinations per month of gestation, from day 90 of pregnancy to foaling were performed. The parameters examined, OD and EV, were used to establish regression models to determine gestational age of mares. The results led to establish linear regressions of the two parameters, EV and OD. The derived gestational age (GA) prediction formulas were: GA = 0,0097EV + 93,887; GA = 8.687OD + 0.6598 (GA in day; EV in mm3; OD in mm). The coefficients of correlation (R) between EV (R=0.96), OD (R=0.93) and mare gestational age were highly significant (P<0.001) indicating that the two eye measurements can be used to determine accurately mare gestational age after 90 days of pregnancy in Arab-Barb breed. In addition, the formula established in the present work from the OD is more accurate in determining the GA in the Arab-Barb compared to that predefined for English Thoroughbred. This comparison reveals that the growth of fetal orbit of Arab-Barb breed is slower than that of the English Thoroughbred.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82355924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}