利用四种伴侣鸟不同生物样本进行DNA性别鉴定

M. Turcu, A. Paștiu, L. Bel, Vlad Cocostîrc, F. Lucaci, D. Pusta
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引用次数: 1

摘要

大多数伴侣鸟没有明显的两性二态特征。本研究旨在从不同组织样本中鉴定鸟类性染色体(ZW)中的冠心病基因。收集了4种伴侣鸟(家鸡、波状斑胸鹦鹉、灰背鹦鹉、阿腊腊鹦鹉)的羽毛、口腔拭子、血液和各种组织(尸检)。使用组织裂解机II对羽毛进行机械加工。使用Isolate II Genomic DNA, Bioline试剂盒进行DNA分离。PCR方法参照Griffiths et al.(1998)使用P2/P8引物进行。根据鸡的表型特征确定了鸡的DNA性别,并通过PCR进行了验证。根据尸检结果,一种虎皮鹦鹉的DNA性别鉴定为雄性和雌性。非洲灰鹦鹉,通过DNA性别鉴定为雌性,根据先前进行的基因性别鉴定确认。这只蓝黄金刚鹦鹉通过DNA性别鉴定被确定为雄性,并根据尸检结果得到了确认。血液样本中的DNA浓度高于口腔拭子或羽毛中的DNA浓度。对鸟类来说,采血被认为是一种侵入性的过程。对于羽毛样品,建议在提取DNA之前进行机械变性。口腔拭子样本适用于分离和扩增鸟类DNA。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DNA Sex Identification Using Different Biological Samples from Four Companion Bird Species
Most companion birds have no distinct sexual dimorphic traits. The aim of this study was to identify the CHD genes from sexual chromosomes of birds (ZW) from various tissue samples. Feathers, oral swabs, blood and various tissues (by necropsy) were collected from four companion bird species (Gallus gallus domesticus, Melopsittacus undulatus, Psittacus erithacus, Ara ararauna). Feathers were processed mechanically using the Tissue Lyser II. DNA isolation was performed using the Isolate II Genomic DNA, Bioline kit. PCR was performed according to Griffiths et al. (1998) using P2/P8 primers. Chicken whose DNA sexing was identified based on phenotypic characters, was confirmed by PCR. Budgerigars whose DNA sexing identified a male and female have been confirmed based on necropsy results. African Grey Parrot whose sex was identified by DNA sexing as female was confirmed based on a genetic sexing previously performed. The Blue and Yellow Macaw, whose sex was determined as male by DNA sexing, was confirmed based on necropsy results. DNA concentration was higher in blood samples than in oral swabs or feathers. Blood collection is considered an invasive procedure in birds. For feather samples, mechanical denaturation before DNA extraction is recommended. Oral swab samples are suitable for isolating and amplifying DNA in birds.
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