Bulletin of The Veterinary Institute in Pulawy最新文献_第2页

Redox status in the blood of ewes in the perinatal period and during lactation 围产期和哺乳期母羊血液中的氧化还原状态

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-12-01 DOI: 10.1515/BVIP-2015-0083

K. Ognik, K. Patkowski, T. Gruszecki, K. Kostro

引用次数: 9

Innervation of the chinchilla testis, epididymis, and vas deferens 龙猫睾丸、附睾和输精管的神经支配

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-12-01 DOI: 10.1515/BVIP-2015-0082

W. Sienkiewicz, A. Szczurkowski, A. Dudek, J. Kaleczyc

{"title":"Innervation of the chinchilla testis, epididymis, and vas deferens","authors":"W. Sienkiewicz, A. Szczurkowski, A. Dudek, J. Kaleczyc","doi":"10.1515/BVIP-2015-0082","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0082","url":null,"abstract":"Abstract The study was performed on six male chinchillas. The animals were anaesthetised with ether and the anaesthesia was deepened with nembuthal injected intraperitoneally. The chinchillas were then transcardially perfused with 0.4 L of 4% buffered paraformaldehyde, and testes, epididymides, and vasa deferentia were collected. The tunica albuginea from one testis from each chinchilla was stained as whole-mount preparation. The tissues were cut into 12 μm-thick cryostat sections, and processed for double-immunofluorescence method. In all organs studied, the most abundant nerve fibres were dopamine β hydroxylase positive (DβH+). Some of them contained neuropeptide Y (NPY). Sporadically NPY-positive-only nerve fibres were found. Single DβH+ nerve terminals contained also galanine. Small numbers of the nerve fibres supplying studied organs were stained for substance P (SP) and calitonin gene related peptide (CGRP). Almost all SP+ fibres were also CGRP+, whereas single CGRP+ nerves were SPimmunonegative. Some nerve terminals were immunoreactive to vesicular acetylcholine transporter and vasoactive intestinal peptide. The organs studied were innervated unevenly. The highest density of the nerves was found in the areas of the tunica albuginea adjacent to the mesorchial border of the testis and their number gradually decreased towards the free border of the gonad. None of the vascular tissue of the testicular parenchyma was free of the nerve fibres, except sporadically encountered DβH+ nerves which supply seminiferous tubules. Within the head of the epididymis a moderate number of nerve terminals were found, but in the body and tail of the organ the number of nerves gradually increased. The vas deferens was supplied with very numerous nerve fibres. There were no differences in the density of the innervation between the funicular and abdominal part of the vas deferens.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"547 - 556"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67316893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Substitution in position 222 of haemagglutinin of pandemic influenza A (H1N1) viruses isolated from pigs in Poland 从波兰猪中分离的甲型H1N1大流行性流感病毒222位血凝素的替换

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-12-01 DOI: 10.1515/BVIP-2015-0066

A. Kowalczyk, K. Urbaniak, I. Markowska-Daniel, Z. Pejsak

引用次数: 0

Application of SYBR Green I and TaqMan probe-based real-time PCRs for the identification of Listeria spp. and Listeria monocytogenes 基于SYBR Green I和TaqMan探针的实时pcr在李斯特菌和单核增生李斯特菌鉴定中的应用

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-12-01 DOI: 10.1515/BVIP-2015-0073

A. Kędrak-Jabłońska, S. Budniak, A. Szczawińska, M. Reksa, M. Krupa, K. Szulowski

{"title":"Application of SYBR Green I and TaqMan probe-based real-time PCRs for the identification of Listeria spp. and Listeria monocytogenes","authors":"A. Kędrak-Jabłońska, S. Budniak, A. Szczawińska, M. Reksa, M. Krupa, K. Szulowski","doi":"10.1515/BVIP-2015-0073","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0073","url":null,"abstract":"Abstract The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes. Five strains of L. monocytogenes and single strains of each of the species: L. ivanovii, L. innocua, L. grayi, L. welshimeri, and L. seeligeri were used for the experiments. Additionally, five strains of other species of bacteria were used for evaluation of the specificity of the tests. QuantiTect SYBR Green PCR and QuantiTect Probe PCR kits were selected for the study. In the first stage of the study, SYBR Green I real-time PCRs were performed under several methods, the first one allowing detection of the 23S rDNA gene and the remainder based on the amplification of the hlyA gene. In the next part, three varied in method TaqMan probe-based real-time PCRs allowing confirmation of strains belonging to Listeria spp. and L. monocytogenes were conducted. The observation of amplification curves in real-time PCR methods enabled the detection of both genes, and these methods demonstrated a significant sensitivity and high specificity. A high regression coefficient of 0.99 was found for all reactions. Specific amplification products were obtained for the 23S rDNA and hlyA genes, which confirmed the tested strains as Listeria spp. and L. monocytogenes respectively. Isolates of other microbial species did not yield real-time PCR products.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"489 - 494"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67315402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Seroprevalence of Coxiella burnetii in Polish cattle herds 波兰牛群伯纳氏科希菌血清流行率

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-12-01 DOI: 10.1515/BVIP-2015-0071

A. Jodełko, K. Niemczuk, M. Szymańska-Czerwińska

引用次数: 7

Anthelmintic residues in goat and sheep dairy products 山羊和绵羊乳制品中的驱虫残留物

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-12-01 DOI: 10.1515/BVIP-2015-0077

P. Jedziniak, M. Olejnik, J. Rola, T. Szprengier-Juszkiewicz

{"title":"Anthelmintic residues in goat and sheep dairy products","authors":"P. Jedziniak, M. Olejnik, J. Rola, T. Szprengier-Juszkiewicz","doi":"10.1515/BVIP-2015-0077","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0077","url":null,"abstract":"Abstract A multiresidue method (LC-MS/MS) for determination of wide range of anthelmintics was developed. The method covered benzimidazoles: albendazole (and metabolites), cambendazole, fenbendazol (and metabolites), flubendazole (and metabolites), mebendazole (and metabolites), oxibendazole, thiabendazole (and metabolites), triclabendazole (and metabolites); macrocyclic lactones: abamectin, doramectin, emamectin, eprinomectin, ivermectin, moxidectin; salicylanilides: closantel, ioxynil, nitroxynil, oxyclosamide, niclosamide, rafoxanid and others: clorsulon, derquantel, imidocarb, monepantel (and metabolites), morantel, praziquantel, and pyrantel. The method was used to examine the potential presence of anthelmintics in goat and sheep milk and dairy products from the Polish market. A total of 120 samples of milk, yoghurt, cottage cheese, cream cheese, and curd were analysed. None of the samples were found positive above CCα (1-10 μg/kg) except for one cottage cheese in which traces of albendazole sulfone were detected (5.2 ug/kg) and confirmed. The results of the study showed negligible anthelmintic residues in the goat and sheep milk and dairy products and confirm their good quality.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"515 - 518"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67315462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Presence of Escherichia coli O157 and O157:H7 in raw milk and Van herby cheese 生奶及凡赫比奶酪中检出大肠杆菌O157及O157:H7

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-12-01 DOI: 10.1515/BVIP-2015-0076

Y. C. Sancak, Hakan Sancak, O. İşleyi̇ci̇

{"title":"Presence of Escherichia coli O157 and O157:H7 in raw milk and Van herby cheese","authors":"Y. C. Sancak, Hakan Sancak, O. İşleyi̇ci̇","doi":"10.1515/BVIP-2015-0076","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0076","url":null,"abstract":"Abstract The Shiga toxin-producing Escherichia coli (STEC) strains are currently considered important emerging pathogens threatening public health. Among Shiga toxin-producing Escherichia coli, E. coli O157:H7 strains have emerged as important human pathogens. This study was conducted to determine the presence of Escherichia coli O157 and O157:H7 in raw milk samples and Van herby cheese samples. For this purpose, 100 samples of raw milk were collected and 100 samples of herby cheese sold for consumption in Van province in Turkey were obtained from grocers and markets in order to detect the presence of Escherichia coli O157 and O157:H7. The method of E. coli O157 and O157:H7 isolation proposed by the Food and Drug Administration (FDA) was used. E. coli O157 in raw milk and herby cheese samples was found in 11% and 6% of samples respectively, and E. coli O157:H7 was found in 2% of herby cheese samples. No E. coli O157:H7 was detected in raw milk samples. This study showed that raw milk was contaminated with E. coli O157 and herby cheese was contaminated with both E. coli O157 and E. coli O157:H7; therefore, herby cheese poses a serious risk to public health.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"511 - 514"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67315642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Usefulness of immature golden hamster (Mesocricetus auratus) as a model for uterotrophic assay 未成熟金仓鼠(Mesocricetus auratus)作为子宫营养试验模型的有效性

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-12-01 DOI: 10.1515/BVIP-2015-0080

L. Radko, M. Minta, A. Jasik, S. Stypuła-Trębas, J. Żmudzki

{"title":"Usefulness of immature golden hamster (Mesocricetus auratus) as a model for uterotrophic assay","authors":"L. Radko, M. Minta, A. Jasik, S. Stypuła-Trębas, J. Żmudzki","doi":"10.1515/BVIP-2015-0080","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0080","url":null,"abstract":"Abstract The present study assessed the sensitivity of immature hamster uterotrophic assay to reference oestrogen agonists/antagonists in order to develop a sensitive model for evaluation of endocrine-active compounds in diets. After performing a baseline for control animals, the sensitivity of immature females (postnatal day 18) to reference compounds was evaluated in a three-day uterotrophic assay. The absolute and adjusted dry uterine weights, fold induction over control for absolute wet uterine weight, and wet uterine weight/body weight ratio (%) were used as endpoints. The significantly active doses for reference oestrogens were as follows: 0.6 μg/kg for 17α-ethinyloestradiol (s.c.); 1 μg/kg/day (s.c.) and 40 μg/kg (p.o.) for diethylstilboestrol; 40 mg/kg (s.c.) and 160 mg/kg (p.o.) for bisphenol A. Co-treatment with tamoxifen at a dose of 1 mg/kg significantly antagonised the uterotrophic effect induced by 1 μg/kg 17α-ethinyloestradiol, and showed the attenuated proliferative effect in histopathological examination. We found immature hamster uterotrophic assay as a sensitive model that could be a good alternative to the rat assay.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"533 - 540"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67316128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Prevalence of bovine herpes virus type 1 in small herds of young beef cattle in south-eastern Poland – a preliminary study 波兰东南部小群年轻肉牛中1型牛疱疹病毒流行率的初步研究

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-12-01 DOI: 10.1515/BVIP-2015-0069

A. Wernicki, R. Urban-Chmiel, J. Rola, W. Socha, D. Stegierska, M. Dec, A. Puchalski

引用次数: 2

Experimental infection with epizootic haematopoietic necrosis virus (EHNV) of rainbow trout (Oncorhynchus mykiss Walbaum) and European perch (Perca fluviatilis L.) 虹鳟(Oncorhynchus mykiss Walbaum)和欧洲鲈鱼(pera fluviatilis L.)感染动物流行性造血坏死病毒(EHNV)的实验研究

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-12-01 DOI: 10.1515/BVIP-2015-0070

E. Borzym, J. Maj-Paluch

引用次数: 2