One health outlook最新文献

{"title":"Correction: Multidrug antibiotic resistance index and antimicrobial resistance patterns of Escherichia coli in intensive pig farms in Kenya.","authors":"Christine Inguyesi, Moses Olum, Peter Ndirangu, Nathan Langat, Ascah Jesang, Ednah Masila, Esther Wachuka, Ruth Onywera, Dishon Muloi, Linnet Ochieng, Victor Yamo, Kelvin Momanyi, Patrick Muinde, John M Maingi, Monicah Maichomo","doi":"10.1186/s42522-026-00249-w","DOIUrl":"10.1186/s42522-026-00249-w","url":null,"abstract":"","PeriodicalId":94348,"journal":{"name":"One health outlook","volume":"8 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection of urban rodents by SARS-CoV-2 in COVID-19 healthcare facilities in Brazzaville, Republic of the Congo.","authors":"Léadisaelle Hosanna Lenguiya, Novy Charel Bobouaka Bonguili, Matthieu Fritz, Deborah Garcia, Amour Mouanda Sounda, Reiche Golmard Elenga, Aldi Fred Mandiangou, Felix Koukouikila-Koussounda, Pembe Issamou Mayengue, N'Kaya-Tobi, Eric M Leroy, Fabien Roch Niama","doi":"10.1186/s42522-026-00224-5","DOIUrl":"10.1186/s42522-026-00224-5","url":null,"abstract":"<p><strong>Background: </strong>Considering the global increase of COVID-19 cases in the healthcare facilities between 2020 and 2021, and the widespread presence of pest rodents in Brazzaville, we conducted this study at the end of the pandemic peak to assess the potential for urban rodents to be infected with SARS-CoV-2 in the Republic of the Congo.</p><p><strong>Methods: </strong>Between April and December 2022, a total of 96 rodent were captured in three COVID-19 healthcare facilities in Brazzaville. Intestinal samples were tested for SARS-CoV-2 RNA using RT-qPCR, followed by nested RT-PCR targeting the RdRp gene and Sanger sequencing. Concurrently, serum samples were analysed to detect SARS-CoV-2 antibodies using the Luminex technology.</p><p><strong>Results: </strong>SARS-CoV-2 RNA was detected in intestinal samples from nine (9.4%) rodent: 7/80 (8.7%) R. rattus, 1/13 (7.7%) R. tanezumi, and 1/2 (50%) M. musculus. Phylogenetic analyses using Nextclade and maximum likelihood phylogeny methods revealed substantial diversity among SARS-CoV-2 partial genome belonging to clades 20B, 20G, Recombinant, 21 K and 22B. Additionally, 9/96 (9.4%) rodents tested seropositive for SARS-CoV-2 antibodies.</p><p><strong>Conclusions: </strong>These findings provide evidence for the establishment of new SARS-CoV-2 reservoirs in certain animal populations, emphasizing the urgent need for continuous surveillance to prevent future outbreaks from animal sources.</p>","PeriodicalId":94348,"journal":{"name":"One health outlook","volume":"8 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13621751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149077021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does plastic waste present an infection hazard? Current evidence, surveillance challenges and research priorities.","authors":"Patrick Ken Kalonde, Kestern Mkoola, Michael Ormsby, Taonga Mwapasa, Precious Innocent Mastala, Madalitso Mphasa, Katalina Tonthola, Fanuel Meckson Bickton, Peter Nambala, Tony Robertson, Richard S Quilliam, Kondwani Chidziwisano, Tracy Morse, Christopher M Jones, Michelle C Stanton, Peter M Atkinson, Marc Y R Henrion, Nicholas Feasey","doi":"10.1186/s42522-026-00237-0","DOIUrl":"https://doi.org/10.1186/s42522-026-00237-0","url":null,"abstract":"<p><p>Unmanaged plastic waste is widespread in settings with limited waste collection, yet its contribution to infectious disease risk remains poorly understood. In this review, we synthesise current evidence on the human health implications of plastic waste, with a specific focus on environmentally persistent bacteria that are transmitted to humans through the faecal oral route. We examine studies documenting microbial colonisation of plastic surfaces, including pathogenic and antimicrobial-resistant bacteria, and assess how well existing human and environmental surveillance systems capture these risks. We also review recent developments in remote sensing for detecting waste accumulation and evaluate spatial and statistical approaches that could link mapped waste to human exposure or infection data. Drawing on this synthesis, we identify major gaps that limit assessment of plastic-associated disease risk and outline key research priorities: improving methods for mapping waste using remotely sensed imagery, characterising spatial and temporal patterns of waste accumulation, and developing exposure metrics that incorporate environmental transport pathways. This review provides a foundation for advancing One Health research on plastic waste and its potential role in disease transmission.</p>","PeriodicalId":94348,"journal":{"name":"One health outlook","volume":"8 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13491761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148804223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One Health in Mesoamerica: historical legacies, current challenges, and strategic directions.","authors":"Bernadeth Cubillo-Cervantes, Mauricio Losilla, Allan Fernández, Jeffry Ortiz-Gamboa, Carlos N Ibarra-Cerdeña, Nadja Kabisch, Jan Felix Drexler, Gerardo Suzán, Andrea Chaves","doi":"10.1186/s42522-026-00236-1","DOIUrl":"10.1186/s42522-026-00236-1","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Mesoamerica, a biodiversity hotspot extending from Mexico to Panama, faces intersecting health challenges rooted in colonial-era transformations and amplified by contemporary environmental change. This perspective paper demonstrates how historical legacies interact with current ecological and social drivers to shape zoonotic disease vulnerability across the region.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Approach: &lt;/strong&gt;We integrate historical analysis of colonial disease introduction with an examination of current environmental, climatic, and social determinants of disease transmission. Findings from a structured multisectoral participatory workshop organized by the GLACIER Project Consortium in Guatemala in 2023, involving participants from academia, government, non-governmental organizations, and community representatives across Mesoamerica, were synthesized together with a narrative review to develop community-centered One Health strategies for the region.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Key findings: &lt;/strong&gt;European colonization of the Americas during the 15th-18th centuries, introduced devastating pathogens and fundamentally altered disease ecology through the introduction of livestock, human-associated pathogens, vectors, and reservoirs, establishing transmission patterns that persist today. Contemporary vulnerabilities arise from: deforestation and agricultural expansion facilitating vector proliferation and wildlife-human contact; informal and unregulated animal movement enabling the spread of transboundary diseases; climate change altering vector distribution and creating novel health threats; fragmented health systems with inequitable access; and poverty-linked structural conditions amplifying disease transmission. Despite growing One Health discourse regionally, meaningful integration across human, animal, plant, and environmental health sectors remains limited, as evidenced by recent disease resurgences and continued institutional gaps in surveillance and cross-border coordination.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Strategic recommendations: &lt;/strong&gt;Six priority action lines emerged from the workshop discussions and their thematic synthesis: strengthening intersectoral governance and regional cooperation; implementing integrated surveillance systems; ensuring community participation and intercultural inclusion of Indigenous knowledge; expanding culturally appropriate health education; promoting community-engaged applied research; and integrating sustainable territorial management. Success requires political commitment beyond economic capacity, positioning communities as decision-makers, and alignment with international frameworks including the FAO-WOAH-WHO-UNEP Joint Plan of Action.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Mesoamerica's interconnected health challenges demand One Health approaches acknowledging historical context while centering vulnerable communities. Regional cooperation, community empowerment, and sustained political will-rather than wealth alo","PeriodicalId":94348,"journal":{"name":"One health outlook","volume":"8 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13465641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148727702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding zoonotic spillover interfaces for the design of a One Health education intervention: a qualitative baseline study from rural Thailand.","authors":"Kate Bärnighausen, Monchai Phongsiri, Kyra Lilier, Simaphon Tomklang, Theerayuth Labooth, Thanapat Chaibut, Pongsakon Jaruton, Kotthamon Chanprathad, Sarutaya Suphawatthanaphan, Hans Overgaard, Mark Donald C Reñosa, Bianca Joyce Sornillo, Jonas Wachinger, Hussein Amin, Mohamed Shelil, Cassia Rocha Pompeu, Uliana Kachnova, Doreen Montag, Vidhya Sasitharan, Till Bärnighausen, Marina Treskova, Patcharin Lapanun","doi":"10.1186/s42522-026-00228-1","DOIUrl":"10.1186/s42522-026-00228-1","url":null,"abstract":"<p><strong>Background: </strong>Prevention of zoonotic spillover requires an understanding of how human, animal, and environmental interactions shape risk in everyday life. Efforts have focused largely on surveillance and response rather than on primary prevention informed by lived experience. In Southeast Asia-a recognised hotspot for zoonotic emergence-there is limited qualitative evidence describing how spillover risk is shaped by routine practices, environmental change, and structural constraints, and how such evidence can inform the design of One Health education interventions.</p><p><strong>Methods: </strong>We conducted a formative qualitative study in Nan Province, northern Thailand, employing shared walks, in-depth interviews, community mapping, and participant and non-participant observation. Data were analysed using reflexive thematic analysis and organised using a qualitative One Health Risk Interface Framework encompassing human-animal, human-environment, human-human, animal-animal and animal-environment interfaces.</p><p><strong>Results: </strong>Spillover risk and mitigation was produced and experienced through routine food preparation, hunting, farming, and caregiving practices. The human-animal interface includes frequent handling of wildlife and domestic animals, mixed-species environments, and informal meat processing. The human-environment interface is characterised by water scarcity, contamination, deforestation, landslides, and inadequate waste management. The human-human interface describes information and communication issues and how these problems can be addressed. The animal-animal and animal-environment interfaces reflect shifting ecologies driven by land-use change, secondary forests, and agricultural intensification, bringing wildlife, livestock, and people into closer proximity.</p><p><strong>Conclusion: </strong>Zoonotic spillover risk in rural Thailand is produced through interconnected behavioural, ecological, and structural processes that cannot be addressed through individual behaviour change alone. An education intervention will need to develop both knowledge and skills within a broader framework of conservation and acknowledgment of structural resource constraints.</p>","PeriodicalId":94348,"journal":{"name":"One health outlook","volume":"8 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13440113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148681963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence, associated risk factors, and molecular detection of Brucella spp. in cattle, sheep, goats and humans in Mvomero district, Morogoro, Tanzania.","authors":"Emmanuel P Lita, Ernatus M Mkupasi, Erneo B Ochi, Gerald Misinzo, Henriette van Heerden, Robab Katani, Jacques Godfroid, Coletha Mathew","doi":"10.1186/s42522-026-00230-7","DOIUrl":"10.1186/s42522-026-00230-7","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Brucellosis is a zoonotic disease of public health and economic importance worldwide. A cross-sectional study using simple random and purposive sampling techniques was conducted from May 2024 to October 2024 in agro-pastoral areas of Mvomero district, Morogoro Region. The aim of this study was to determine the seroprevalence of brucellosis, associated risk factors, and characterize the circulating Brucella spp., in livestock and humans.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 918 serum samples were collected from Mvomero district, comprising cattle (n = 339), sheep (n = 164), goats (n = 303), and humans (n = 112). Animal samples were obtained from 18 herds with mixed livestock species, while human samples were collected from four health facilities. All samples were screened for Brucella-specific antibodies using the Rose Bengal plate test (RBPT), and positive samples were subjected to competitive enzyme-linked immunosorbent assay (c-ELISA). Genomic DNA was extracted from the heparin-coated blood samples corresponding to the same samples that tested positive by c-ELISA for confirmation by using real-time polymerase chain reaction (qPCR). A herd was considered Brucella seropositive if at least one animal tested positive on c-ELISA. A structured questionnaire was used to collect information on potential individual and herd-level risk factors. A multivariate logistic regression analysis was carried out using a backward elimination procedure to identify potential risk factors, following a univariate analysis, using a p-value cut-off of 0.25 from the univariable analysis to select candidate variables for a multivariable model.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;At the individual level, screening by RBPT revealed seropositivity rates of 9.1% (31/339) in cattle, 4.3% (7/164) in sheep, 5.6% (17/303) in goats, and 10.7% (12/112) in humans. Subsequent results by c-ELISA demonstrated lower seroprevalence rates of 6.5% (22/339) in cattle, 1.2% (2/164) in sheep, and 2.0% (6/303) in goats. Human seroprevalence by c-ELISA was 4.5% (5/112), with all positive cases originating from a single health facility. At the herd level, the overall seroprevalence was 55.5% (10/18) based on c-ELISA results. Out of the 35 c-ELISA positive samples, 21/35 (60.0%) tested positive for the Brucella genus detection using the IS711 gene. The Brucella species-specific assay detected 17/35 (48.6%) samples as B. abortus. There was no B. melitensis detected in this study. Risk factor analysis in sheep, goats, and humans yielded no significant associations in the univariable analyses. However, multivariable logistic regression analysis in cattle identified two significant risk factors: prior history of abortion (p &lt; 0.001, OR = 12.7, 95% CI: 3.88-41.8) and retained placenta (p = 0.023, OR = 32.5, 95% CI: 1.602-660.8).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Brucella abortus was the predominant circulating species in cattle, goats and humans. The significant ass","PeriodicalId":94348,"journal":{"name":"One health outlook","volume":" ","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148498595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A scoping review of One Health interventions 2005-2025: What has been done, are they effective, and what are the best practices?","authors":"Eri Togami, Lincoln Hopkins, Lori Rosman, Meghan F Davis","doi":"10.1186/s42522-026-00229-0","DOIUrl":"10.1186/s42522-026-00229-0","url":null,"abstract":"<p><strong>Background: </strong>One Health research and practice are evolving rapidly, necessitating solid evidence-building and quality assessment of One Health interventions. We conducted a scoping review to (1) characterize how many and what kinds of One Health interventions are published in peer-reviewed literature; and (2) assess whether the identified interventions demonstrated effectiveness. We hypothesized that the evidence was limited in numbers and ability to demonstrate effectiveness.</p><p><strong>Methods: </strong>The review was reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews. The initial search was conducted on Medline (Ovid), Embase, PubAg, and Web of Science in May 2024 and updated in December 2025. Inclusion criteria were: Primary research in which the investigators intervened with humans, animals, or their shared environment (at least two of three domains); an outcome assessment was included, and full text was available in English, published on or before December 16, 2025. Two researchers independently screened and analyzed all manuscripts.</p><p><strong>Results: </strong>Over 18,000 references were screened, and 151 studies were included. Studies were conducted in 53 countries representing all six WHO Regions. The highest number of interventions were reported from the United States of America, Tanzania, and India. Among 79 interventions that targeted zoonoses, the most common diseases were general zoonoses, rabies, and cysticercosis. Over half (55%) of the study designs were quasi-experimental, followed by 23% randomized control trials (RCTs) and 19% non-randomized experiments. Less than half (41%) of the studies had a control or comparison group. The most common perceived sources of bias were immigrative (28%) and emigrative selection bias (11%). Eight field-based RCTs identified that a One Health intervention was effective in reducing disease occurrence or pathogen detection compared to the control. Exemplary practices included a comprehensive intervention spanning human, animal, and environmental domains, using a participatory approach, assessing longer-term outcomes, triangulating results using more than one method, not overstating findings, and providing a visual aid to describe the intervention.</p><p><strong>Conclusions: </strong>A small number of field-based RCTs clearly demonstrated the comparative effectiveness of One Health interventions, while it was challenging to assess the effectiveness of other studies without a more granular definition of effectiveness. Rigorous studies that assess the effectiveness of One Health interventions will collectively improve buy-in from governments, researchers, and policymakers, and advance the evidence base for employing the One Health approach.</p>","PeriodicalId":94348,"journal":{"name":"One health outlook","volume":" ","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148475322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological investigation of zoo animals: SARS-CoV-2 antibodies detected in a European moose and an Asian golden cat.","authors":"Kerstin Wernike, Dominik Fischer, Lisa Grund, Martin Beer","doi":"10.1186/s42522-026-00227-2","DOIUrl":"10.1186/s42522-026-00227-2","url":null,"abstract":"<p><p>As the human COVID-19 pandemic progressed, the causative agent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was detected in an increasing number of animal species, raising concerns about the establishment of new animal reservoirs. Here, we investigated the presence of SARS-CoV-2 antibodies in serum samples of 123 mammals housed in various zoological institutions in Germany with direct or indirect contact to humans (visitors and zoo staff), collected between December 2019 and January 2025. The animals included diverse representatives from the orders Artiodactyla (n = 59), Carnivora (n = 54), as well as Proboscidea (n = 7), two perissodactyls, and a rodent. All serum samples were tested using a species-independent commercial surrogate virus neutralization test (sVNT) and a multispecies receptor-binding domain (RBD)-based ELISA. With two exceptions, all samples tested consistently negative in both assays. However, antibodies against SARS-CoV-2 were detected in a European moose (Alces alces) and an Asian golden cat (Catopuma temminckii), sampled in 2020 and 2021, respectively, expanding the known host range of SARS-CoV-2. Hence, pathogen monitoring and continued health surveillance in both captive and wild animal populations are essential for understanding the actual host range, potential reservoir formation, and the broader implications for animal and public health.</p>","PeriodicalId":94348,"journal":{"name":"One health outlook","volume":" ","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13326025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Empowering One Health with metagenomics.","authors":"Erin Harvey, Kate Van Brussel, Edward C Holmes","doi":"10.1186/s42522-026-00225-4","DOIUrl":"10.1186/s42522-026-00225-4","url":null,"abstract":"<p><p>In an increasingly connected world a global One Health approach to the management of human, animal and ecosystem health will be critical to effective infectious disease responses. The emergence and rapid global spread of several emerging and re-emerging pathogens in the past decade has highlighted the need for rapid, sensitive and accurate diagnostics. Metagenomics, while commonly used for research purposes for almost two decades, entered the global spotlight during the COVID-19 pandemic. In this review we discuss the impacts that metagenomic studies have had on our understanding of origins, aetiology and ecology of infectious diseases within a One Health context. We also discuss the role of metagenomics in the future of diagnostics and disease surveillance, and outline the challenges and limitations of current metagenomic methods.</p>","PeriodicalId":94348,"journal":{"name":"One health outlook","volume":" ","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13621691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Winter migratory birds may carry diverse antimicrobial resistance genes into Japan.","authors":"Kei Nabeshima, Manabu Onuma","doi":"10.1186/s42522-026-00223-6","DOIUrl":"10.1186/s42522-026-00223-6","url":null,"abstract":"<p><strong>Background: </strong>Environmental surveillance of antimicrobial resistance (AMR) in wildlife remains limited, despite increasing recognition that resistance determinants can circulate across human, livestock, and natural ecosystems. Migratory waterbirds move long distances and aggregate at shared stopover and wintering sites, potentially facilitating the acquisition and redistribution of antimicrobial resistance genes (ARGs) across regions. However, nationwide evidence describing the breadth of ARGs carried by winter migratory birds in Japan is scarce. We assessed the diversity and distribution of ARGs in pooled fecal samples from winter migratory birds across Japan.</p><p><strong>Methods: </strong>We analyzed pooled fecal DNA collected at migratory bird habitats across 12 local governments during the 2021-2022 and 2022-2023 winter seasons (24 pools). Avian host origin was inferred by DNA metabarcoding, and ARGs were profiled by probe-based target enrichment with read-based detection (ARG detected at ≥ 10 reads).</p><p><strong>Results: </strong>Ducks (Anas spp. and Mareca spp.) were the predominant inferred hosts. ARGs were detected in all areas and included genes associated with resistance to multiple antibiotic classes used in livestock production. Across the two seasons, genes associated with resistance to gentamicin, cephalosporins, macrolides, tetracyclines, fosfomycin, clindamycin, penicillins, streptogramins, sulfonamides/trimethoprim, colistin, erythromycin, chloramphenicol, rifampicin, and isoniazid were detected in all 12 areas in at least one season. Genes associated with resistance to agents restricted for use in Japanese livestock production, including colistin, erythromycin, chloramphenicol, and rifampicin, were also detected in all 24 pools. Isoniazid-, gentamicin-, meropenem-, and tigecycline-associated genes were detected in 23/24, 20/24, 11/24, and 9/24 pools, respectively.</p><p><strong>Conclusions: </strong>These data indicate widespread environmental occurrence of diverse ARGs and support the possibility that migratory birds could contribute to long-distance dispersal of ARGs. Culture-based isolation, phenotypic testing, and quantitative analyses will be needed to identify host bacteria and assess clinical relevance.</p>","PeriodicalId":94348,"journal":{"name":"One health outlook","volume":" ","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13520448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148274329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}