Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer最新文献

{"title":"Construction of a Prognostic Model and Drug Prediction Based on Endoplasmic Reticulum Stress Response Genes in Esophageal Cancer.","authors":"Jianwei Cao, Chaowei Li, Junmeng Xiao, Lei Zhu","doi":"10.1615/JEnvironPatholToxicolOncol.2025054000","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2025054000","url":null,"abstract":"<p><p>Endoplasmic reticulum stress (ERS) has profound implications for various cancer cells, including esophageal cancer (ESCA) cells. Currently, there is no study about the function of ERS-related genes (ERSRGs) prognosis in ESCA. We developed an ERS risk scoring model by TCGA-ESCA dataset to validate predictive ability of ERSRGs for ESCA prognosis and to screen reliable prognostic biomarkers. Enrichment analysis demonstrated that differentially expressed ERSRGs were mainly associated with the ERS response, regulation of apoptosis signaling pathways, and the p53 signaling pathway. Major differences among ESCA patients with different ERS risk scores were found by survival analysis. According to mutation analysis, high-risk group had a higher mutation frequency than the other group. Drug prediction indicated that fulvestrant, elesclomol, and methylprednisolone were promising small molecule candidates that could potentially target model genes. In summary, we developed ERS risk score model serving as an independent prognostic factor and simultaneously predicted several potential small molecule drugs. These results offer new directions for future investigation on prognosis and treatment.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"45 2","pages":"67-78"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene Expression Patterns in Liquid-Liquid Phase Separation: A Prognostic and Predictive Tool for Colorectal Cancer.","authors":"Zelai Liao, Wujun Zou, Jingquan Guo, Xianglong Teng","doi":"10.1615/JEnvironPatholToxicolOncol.2025055725","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2025055725","url":null,"abstract":"<p><p>To illuminate the role of liquid-liquid phase separation (LLPS) in colorectal cancer (CRC) and its potential as a therapeutic target, we conducted an in-depth analysis focusing on LLPS-related genes (LLPSRGs). Our approach involved a predictive model with LLPSRGs levels a s basis, with intent to prognosticate patient survival and assist in immunotherapy decision-making. Employing univariate Cox regression equipped with LASSO for initial screening, we identified LLPSRGs linked to CRC survival. A subsequent multivariate Cox regression analysis led to the establishment of a prognostic model that included seven pivotal genes. Survival analysis and ROC curve assessment confirmed the accuracy of this model in predicting CRC patient survival, validated in a GEO dataset. PPI network showed that there were complex correlations between differentially expressed genes in risk groups. qRT-PCR findings showed that the top 10 hub genes in the network were all upregulated in CRC cells. Immune profiling unveiled differences in immune cell infiltration and potential immunotherapy responses between high-risk and low-risk groups, with the latter being more suitable for immunotherapeutic interventions. Drug sensitivity analysis supported personalized treatment approaches. Overall, we developed a robust LLPSRG-based prognostic model for CRC, offering insights for CRC clinical prognosis and therapy.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"45 2","pages":"31-46"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased hsa_circ_0075829 Facilitates the Progression of Gastric Cancer and Its Relationship with Helicobacter Pylori Infection.","authors":"Zhentao Li, Haihan Zhang, Zhuangmei Wang","doi":"10.1615/JEnvironPatholToxicolOncol.2025054934","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2025054934","url":null,"abstract":"<p><strong>Background: </strong>Circular RNAs (circRNAs) play a pivotal part in the advancement of multiple tumors. Nonetheless, the influence of Helicobacter pylori (H. pylori) infection on the expression of circRNA in gastric cancer remains less studied.</p><p><strong>Aim: </strong>The objective of this research is to clarify the function and underlying mechanism of circ_0075829 in the context of gastric cancer and its relation to H. pylori infection.</p><p><strong>Methods: </strong>The circ_0075829 expression in tissue specimens and cells was evaluated utilizing quantitative real-time PCR (RT-qPCR). H. pylori infection and transfection were treated in gastric cancer cells to measure circ_0075829 expression changes and their effects on cellular behaviors. Kaplan-Meier curve was conducted to assess the clinical prognostic performance of circ_0085729 in patients with gastric cancer. Bioinformatics and dua-luciferase reporter assay were performed to validate the downstream miRNA and mRNA. CCK-8 proliferation assay and transwell experiments were carried out to investigate the influence of circ_0075829-miR-149-5p-AGO1 on cellular activities.</p><p><strong>Results: </strong>Circ_0075829 expression was increased in gastric cancer and correlated with several clinical characteristics and shorter overall survival. Besides, H. pylori infection increased circ_0075829 expression in cancer cells. miR-149-5p was a direct target miRNA of circ_0075829 and was downregulated in gastric cancer. Downregulation of circ_0075829 could repress the proliferative abilities, migratory capacities, and invasion potential of cancer cells by mediating miR-149-5p to regulate AGO1 expression.</p><p><strong>Conclusion: </strong>Circ_0075829 seems to play an oncogenic role in H. pylori-associated gastric malignancies and may serve as a promising indicator for predicting outcomes in gastric cancer, which underpins a more theoretical basis for the progression of new therapeutic approaches to treating gastric cancer.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"44 4","pages":"1-13"},"PeriodicalIF":2.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145491372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Cancer Role of Ellagic Acid by Modulating the Altered PI3K/PTEN/Akt Pathway in Bladder Cancer.","authors":"Satya Sahay, Deepika Trehan, Ranbala Kumari, Jyoti Sharma, Pawan Vasudeva, Niraj Kumar, Usha Agrawal","doi":"10.1615/JEnvironPatholToxicolOncol.2024053383","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2024053383","url":null,"abstract":"<p><p>Bladder cancer (BCa) is approximately the fourth most prevalent diagnosed cancer in men and is three times less common in women. Therefore, identifying biomarkers, developing more effective therapeutic strategies, and understanding the mechanisms underlying BCa tumor growth and progression are urgently required to improve survival rates. Therefore, we aim to investigate the expression of PTEN/Akt in tissue samples of both non-muscle invasive bladder cancer (NMIBC) and muscle-invasive bladder cancer (MIBC) patients (n = 70) and human BCa cell lines T24 and 5637, along with the potent role of ellagic acid (EA) in the modulation of the PTEN/Akt pathway and the resulting therapeutic potential. Results showed low-intensity nuclear or cytoplasmic PTEN staining or loss of PTEN expression in tumor cells and overexpression of p-Akt (Ser-473) with high intensity in the nucleus or cytoplasm. EA treatment of T24 and 5637 cells reduced cell viability, inflammation (NF-κB, COX-2), invasion (MMP-9), induced the caspase (cas-3 and cas-9) cascade signaling pathway, and induced cell apoptosis along with the suppression of the PI3K/PTEN/Akt signaling pathway after 48h in a dose-dependent manner. Thus, these data suggested that the EA showed a strong potential anti-cancer effect in T24 and 5637 cells. In conclusion, the expression of PTEN/p-Akt and the inverse relation indicated an alteration of the PTEN/Akt pathway, and such cases could benefit from treatment with EA in BCa.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"44 2","pages":"57-69"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Riskscore Model for Predicting Survival, Tumor Microenvironment, Immunotherapy and Drug Sensitivity of Lung Squamous Cell Carcinoma Based on PI3K/AKT/MTOR Pathway-Related Genes.","authors":"Qiang Zhang, Jianhua Zhu, Huiyong Han","doi":"10.1615/JEnvironPatholToxicolOncol.2025054954","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2025054954","url":null,"abstract":"<p><p>Targeting PI3K/AKT/MTOR (PAM) signaling pathway may be a strategy at the fore for treating lung squamous cell carcinoma (LUSC). However, relationships of PAM pathway-related genes (PAGs) with LUSC prognosis are unknown. Therefore, identifying the prognostic significance of PAGs for LUSC is innovative and feasible. Transcriptomic data, clinical features, and PAGs of LUSC were obtained from public databases (TCGA, GEO). A PAGs-based prognostic model was built using regression analysis in TCGA-LUSC. Gene levels were assessed via qRT-PCR. Predictive performance was verified through multiple datasets. Differences in immune infiltration and anti-tumor immunity between risk groups were assessed by R packages. Sensitivity to common anti-cancer agents was tested using oncoPredict package. We identified a Riskscore model containing 11 PAGs. Patients were assigned into groups of high risk (HR) and low risk (LR) per median Riskscore. CAB39L, CDKN1A, and ITPR2 were significantly underexpressed in LUSC cells. TRAF2 and TRIB3 were significantly enhanced in LUSC cells. The LR group had a longer survival time. Prognostic values of one-, three-, and five-year ROC curves were good. Results were verified in GEO. Patients in LR group had higher immune infiltration levels of B cells and Tfh cells, and higher ssGSEA scores for APC_co_inhibition and T_cell_ co_stimulation. LR group had lower TIDE scores and lower IC50 values (Alpelisib, Ibrutinib, Sapitinib, and Savolitinib). We successfully built a reliable 11-gene Riskscore prognostic model. Patients in LR group had potential advantages in survival, immune response, and drug sensitivity. In summary, the results offered new insights into prognosis prediction, immunotherapy, and personalized treatment of LUSC.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"44 4","pages":"15-29"},"PeriodicalIF":2.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145491341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"lncRNA-miRNA/RBP-mRNA Network Involved in Human Triple-Negative Breast Cancer: An Integrated Approach.","authors":"Wen Yun, Yao Li, Jianyuan Huang, Yuan Yuan","doi":"10.1615/JEnvironPatholToxicolOncol.2024056566","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2024056566","url":null,"abstract":"<p><p>Noncoding RNA regulatory networks play crucial roles in human breast cancer. The aim of this study was to establish a network containing multi-type RNAs and RBPs in triple-negative breast cancer (TNBC). Differential expression analyses of lncRNAs, miRNAs, and genes were performed using the GEO2R tool. Downstream RBPs and miRNAs were identified using respective databases. GO, KEGG, and protein-protein interaction were predicted. Expression of mRNAs, lncRNAs, and miRNAs were examined using TCGA and ENCORI. TMEM161B-AS1-miR-3646-SERPINB5 axis was validated with the expression level and cell function. TMEM161B-AS1-RBFOX1-SER-PINB5 axis was validated using RIP assays. A lncRNA-miRNA-mRNA network, a lncRNA-RBP-mRNA network, and a lncRNA-miRNA/RBP-mRNA network were constructed. Alterations in the expression levels of TMEM161B-AS1, miR-3646, and SERPINB5 were confirmed by real-time polymerase chain reaction as downregulation, upregulation, and downregulation, respectively. TMEM161B-AS1 inhibited TNBC cell proliferation, migration, and invasion. miR-3646 reversed the inhibitory effect of TMEM161B-AS1 on cell function, while SERPINB5 offset the miR-3646 effect. RB-FOX1 bound to SERPINB5 mRNAs, and TMEM161B-AS1 was involved in their interaction. This study revealed the ln-cRNA-miRNA/RBP-mRNA network in TNBC, providing more directions to study the molecular mechanism of TNBC.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"44 3","pages":"75-87"},"PeriodicalIF":2.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145025078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory Gene Variants and Protein Levels: An Important Predictor of Prostate Cancer Development.","authors":"Emine Yagci, Hulyam Kurt, Ata Ozen, Cansu Ozbayer, Ertugrul Colak","doi":"10.1615/JEnvironPatholToxicolOncol.2024054333","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2024054333","url":null,"abstract":"<p><p>Prostate cancer and inflammation mechanism are closely related because chronic inflammation causes inflammatory cells to infiltrate into prostatic atrophy areas and proliferative inflammatory atrophy is accepted as the initiator of prostate cancer. The study included 90 patients (28 patients with benign prostatic hyperplasia (BPH), 35 patients with localized prostate cancer (LPCa), and 27 patients with metastatic prostate cancer (MPCa) and 90 healthy controls. Blood samples from 90 patients and 90 healthy people were used to isolate genomic DNA. Serum protein levels were detected using the ELISA technique, and genotyping was done using the PCR-RFLP method. It was found that genotype distributions of CCR3 gene rs4987053 and the COX-2 gene rs689466 variants showed a substantial difference between the groups (Control, BPH, LPCa, MPCa) (respectively P < 0.05, P < 0.001). In addition, a important difference was determined between the non-cancerous and the prostate cancer groups in the NOD1 gene rs5743336 variant genotype distributions (P < 0.05). However, no substantial relationship was determined between the rs16969415, rs1801157, rs2228014, rs2066847, rs4986791 variants, and a risk of prostate cancer. The serum IL-1β, LY96, and TLR4 protein levels differed significantly between the groups (Control, BPH, LPCa, MPCa) (P < 0.001). In addition, IL-1β was associated with rs689466, LY96 with rs2228014, rs5743336 genotypes (P < 0.05). As a conclusion, the CCR3 gene rs4987053, COX-2 gene rs689466, and NOD1 gene rs5743336 variations were determined to be closely associated with prostate cancer risk.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"44 3","pages":"27-49"},"PeriodicalIF":2.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145025083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Interleukin-1 Receptor-Associated Kinase-1 by Pacritinib Is a Therapeutic Strategy to Overcome Melanoma Resistance.","authors":"Li Ma, Yue Ruan, Mei Cao, Jing Ke","doi":"10.1615/JEnvironPatholToxicolOncol.2024053298","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2024053298","url":null,"abstract":"<p><p>Despite advancements in systemic therapy, the mortality rate for patients with metastatic melanoma remains around 70%, underscoring the imperative for alternative treatment strategies. Through the establishment of a chemoresistant melanoma model and a subsequent drug investigation, we have identified pacritinib, a medication designed for treating myelofibrosis and severe thrombocytopenia, as a potential candidate to overcome resistance to melanoma therapy. Our research reveals that pacritinib, administered at clinically achievable concentrations, effectively targets dacarbazine-resistant melanoma cells by suppressing IRAK1 rather than JAK2. Notably, melanoma-resistant cells exhibit elevated p-IRAK1 levels, while there is no discernible difference in p-JAK2 levels between sensitive and resistant cells. Additionally, analysis of melanoma patients indicates a higher expression of IRAK1, correlating with a significantly improved survival rate. Furthermore, the depletion of IRAK1 not only reduces cell viability in melanoma cells but also enhances the effectiveness of dacarbazine treatment. In a dacarbazine-resistant melanoma model, pacritinib demonstrates significant inhibition of tumor growth in mice, leading to enhanced overall survival. In the dacarbazine-sensitive melanoma model, the combination of pacritinib and dacarbazine is more effective than dacarbazine alone in reducing tumor growth and improving overall survival in mice. In summary, our findings highlight IRAK1 as a promising therapeutic target to overcome melanoma resistance, and pacritinib emerges as a valuable addition to the treatment armamentarium for melanoma.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"44 3","pages":"51-61"},"PeriodicalIF":2.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145025072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"lncRNA PTCSC1 Promotes TRAIL Resistance through FOXO3a Pathway in HCT116 and SW480 Cells.","authors":"Changcheng Wang, Jia Guo, Zengan Wu","doi":"10.1615/JEnvironPatholToxicolOncol.2024053010","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2024053010","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a malignant tumor that affects patients worldwide, and its mortality rate is high. Although treatments that activate TNF-associated apoptosis-inducing ligand (TRAIL)-induced apoptosis have shown some efficacy, many CRC patients are resistant to TRAIL therapy. Our findings indicated that the lncRNA PTCSC1 is over-expressed in CRC. However, the mechanism underlying resistance to PTCSC1 in CRC is unclear. In this work, we determined the role of PTCSC1 in TRAIL-resistant CRC patients and explored possible molecular mechanisms. We found that TRAIL-sensitive HCT116 and SW480 cells expressed relatively lower levels of PTCSC1 than TRAIL-resistant HT-29 and caco-2 cells. Increased expression of PTCSC1 was here found to inhibited TRAIL-induced apoptosis in HCT116 and SW480 cells. Decreased expression of PTCSC1 increased TRAIL-induced apoptosis in HT-29 and caco-2 cells. The level of expression of PTCSC1 was related to their sensitivity to TRAIL-induced apoptosis. Furthermore, PTCSC1 decreased the expression of Death Receptor 4 (DR4) while increased the activation of serine/threonine kinase 1 (AKT) and Forkhead Box O3a (FOXO3a). Our findings therefore support the idea that targeting PTCSC1 function may represent a strategy to overcome TRAIL resistance in CRC through the DR4/AKT/FOXO3a pathway.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"44 2","pages":"31-39"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiotherapy-Related Autophagy Genes Predict Prognosis and Reveal Immunoscape Features and Immunotherapeutic Agents in Colorectal Cancer Patients.","authors":"Mao Mao, Yichao Zhuang, Yan Chen","doi":"10.1615/JEnvironPatholToxicolOncol.2025055727","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2025055727","url":null,"abstract":"<p><p>Numerous genes have been associated with colorectal cancer (CRC) treatment in prior studies, but the impact of radiotherapy-related autophagy genes (RRAGs) on CRC remains largely unexplored. The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were implemented to screen out RRAGs significantly associated with survival, and a prognostic model was constructed. Samples were categorized into high- and low-risk groups with median riskscore. Immunomicroenvironment analysis, immunotherapy response prediction, enrichment analysis, tumor mutation analysis and drug prediction were performed in risk groups. Expression of signature genes in CRC cells was examined using quantitative reverse transcription polymerase chain reaction (qRT-PCR). There were 10 CRC-related RRAGs found. Prognostic models were built with RRAGs. Based on immune infiltration analysis, low-risk populations showed significantly greater levels of immune infiltration (P P ARHGEF17 mutation rate was 6%. Medications such as CGP-082996, Dasatinib, Erlotinib, and Salubrinal were more sensitive to high-risk group, whereas drugs such as FTI-277, DMOG, and Crizotinib were more sensitive to low-risk group. UGT1A6 and IRGM were significantly upregulated in tumor group as revealed by qRT-PCR. This study constructed a new prognostic model for CRC patients based on RRAGs, and a series of analysis results is conducive to providing more theoretical references and new insights into precision treatment of CRC patients.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"44 4","pages":"63-80"},"PeriodicalIF":2.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145490439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}