Experimental oncology最新文献

{"title":"IDENTIFICATION AND FUNCTIONAL CHARACTERIZATION OF MICRORNAS REGULATING HTERT.","authors":"Maham Ansari, Rafiullah Rafiullah, Abdul Wali, Afrasiab Khan Tareen, Imrana Niaz Sultan, Muhammad Mushtaq Yasinzai","doi":"10.15407/exp-oncology.2025.02.167","DOIUrl":"https://doi.org/10.15407/exp-oncology.2025.02.167","url":null,"abstract":"<p><strong>Background: </strong>Telomerase is a ribonucleoprotein (RNP) reverse transcriptase that replicates the ends of chromosomes, thereby maintaining genome integrity, and its inhibition may be envisioned to prevent carcinogenesis or treat cancer patients. Various approaches have been used to target hTERT, and one of the promising strategies is the use of hTERT-targeting microRNAs (miRNAs).</p><p><strong>Aim: </strong>To investigate the interaction of miRNAs with hTERT, describing the strength, affinity, preferred binding orientation, and in vitro verification of miRNA on hTERT expression in cancer.</p><p><strong>Materials and methods: </strong>The miRWalk, TargetScan, and miRDB databases were used for screening. Consistently, five top-hit miRNAs were found in all three databases that could interact with hTERT mRNA, namely, hsa-miR-4651, hsa-miR-608, hsa-miR-6796-5p, hsa-miR-6752-5p, and hsa-miR-6791-5p. We applied stringent in silico tools to firstly model the structures of lead miRNA and hTERT mRNA. Then docking was performed, and finally stability of miRNA-mRNA complexes was analyzed using MD simulations.</p><p><strong>Results: </strong>The expression of the selected miRNAs was inhibited in the MCF-7 breast cancer cell line. The inhibition of hsa-miR-6796-5p was enhanced, while hsa-miR-4651 significantly reduced the expression of hTERT protein. Moreover, the inhibition of hsa-miR-4651 expression led to a reduction in melanoma and breast cancer cell proliferation.</p><p><strong>Conclusion: </strong>The current study provided a detailed procedure for identifying and verifying miRNAs against mRNAs, as well as highlighting the differential regulation of hTERT by specific miRNAs. It demonstrated that miRNA inhibition can modulate hTERT expression and cell proliferation, with potential implications for targeted cancer therapies. The strategy used in this study could also be applied to other genes for screening potential miRNAs.</p>","PeriodicalId":94318,"journal":{"name":"Experimental oncology","volume":"47 2","pages":"167-180"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOLECULAR MARKERS IN PREDICTING THE OUTCOME OF DIFFUSE GLIOMA GRADE 4 TREATMENT.","authors":"O Glavatskyi, I Vasylieva, T Malysheva, N Chopik, O Tsiubko, I Shuba, A Shmelova, O Zemskova, L Yakovenko, E Pedachenko","doi":"10.15407/exp-oncology.2025.02.216","DOIUrl":"https://doi.org/10.15407/exp-oncology.2025.02.216","url":null,"abstract":"<p><strong>Aim: </strong>To assess the expression of MGMT (O6-methylguanine-DNA methyltransferase) gene by MGMT RNA abundance and the presence of IDH1/2 (isocitrate dehydrogenase) variants in glioblastoma (GBM) samples for predicting the efficacy of temozolomide (TMZ) treatment, recurrence risk, and patients' survival.</p><p><strong>Materials and methods: </strong>The expression of the MGMT gene and the presence of IDH1/2 variants were assessed by RT-PCR in tumor samples from 39 patients with histologically verified GBM or diffuse astrocytoma, grade 4. The number of MGMT RNA copies was determined by the calibration curves based on the pMA-RQ plasmid with the inserted MGMT gene.</p><p><strong>Results: </strong>The number of MGMT RNA copies in GMB samples varied broadly from 1.7 to 88,270.2 copies per 1000 cells. The patients with a low level of MGMT expression (<1000 copies) in tumors had a more favorable prognosis for the TMZ treatment compared to the patients with a high level of MGMT RNA abundance (>10,000 copies). Among the patients included in the study, a wild type of IDH1/2 was detected in 36 cases, while 3 cases were IDH1 heterozygous.</p><p><strong>Conclusion: </strong>The level of MGMT expression is considered a significant factor for prognosing GMB patients' survival. Patients with a low level of MGMT expression are considered candidates for efficient therapy with alkylating agents.</p>","PeriodicalId":94318,"journal":{"name":"Experimental oncology","volume":"47 2","pages":"216-222"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LYMPHOCYTE SUBSET DISTRIBUTION AFTER COMBINED CHEMO- AND RADIOTHERAPY IN PATIENTS WITH CANCER OF THE ORAL CAVITY, OROPHARYNX, AND LARYNGOPHARYNX.","authors":"M Vorobyov, L Zvarych, D Bazyka","doi":"10.15407/exp-oncology.2025.02.207","DOIUrl":"https://doi.org/10.15407/exp-oncology.2025.02.207","url":null,"abstract":"<p><strong>Aim: </strong>To study the prognostic value of the lymphocyte subset distribution to predict the overall survival and its association with the clinicopathologic characteristics and treatment in patients with cancer of the oral cavity, oropharynx, and laryngopharynx.</p><p><strong>Materials and methods: </strong>44 patients were examined. Immunophenotyping of lymphocyte subsets was performed in peripheral blood samples using flow cytometry. The lymphocyte subset distribution was analyzed depending on the clinicopathological characteristics and treatment outcome, as well as the overall survival.</p><p><strong>Results: </strong>The changes in CD3+ T-cells and CD3+57+ NKT counts were associated with the sex of the patients, TCRαβ+ T-cells - with the stage of the disease, CD4+8+T-cells and CD3-16+57+ NK - with the tumor size and differentiation grade, and CD3+HLA-DR+,CD8+ T-cells, and CD4+/CD8+ ratio - with lymph node involvement. The content of CD3+HLA-DR+ and TCRαβ+ T-cells, CD3-16+57+ NK, and CD3+57+ NKT differed in patients depending on the tumor location. There were changes in CD19+ and HLA-DR+ B-cells, CD3+, CD4+, CD4+25+ and TCRαβ+ T-cells, CD3-CD16+57+ NK, and CD3+57+ NKT during treatment, with the most pronounced changes after the first stage of RT. The relative number of CD3+HLA-DR+ and tumor size T4 influenced the overall survival of patients ((HR = 0.798, 95% CI, 0.658-0.967, p = 0.021) and (HR = 3.015, 95% CI, 1.303-6.975, p = 0.009), respectively).</p><p><strong>Conclusion: </strong>Parameters of lymphocyte subsets can be promising prognostic markers.</p>","PeriodicalId":94318,"journal":{"name":"Experimental oncology","volume":"47 2","pages":"207-215"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INTERPLAY OF EPIGENETIC REGULATION OF KI-67 AND P53 BY MIR-21 AND MIR-34A IN CERVICAL INTRAEPITHELIAL NEOPLASIA.","authors":"A Svintsitska, N Lygyrda, V Svintsitskyi, T Borikun, A Kryzhanivska","doi":"10.15407/exp-oncology.2025.02.223","DOIUrl":"https://doi.org/10.15407/exp-oncology.2025.02.223","url":null,"abstract":"<p><strong>Background: </strong>Cervical cancer (CC), primarily linked to persistent HPV infection, arises from complex genetic and epigenetic alterations. The early detection of cervical intraepithelial neoplasia (CIN) allows for CC prevention. Recent data highlights the importance of epigenetic biomarkers, including non-coding RNAs such as miR-21 and miR-34a. Our aim was to investigate the interplay between Ki-67 and p53 expression and their epigenetic regulation by miR-21 and miR-34a to better predict the course of CIN.</p><p><strong>Materials and methods: </strong>Tumor biopsies from 50 patients with CIN 1-3/HSIL were analyzed. We performed immunohistochemical analysis of Ki-67 and p53 expression and qRT-PCR for the analysis of miRNA expression.</p><p><strong>Results: </strong>The average miR-21 and miR-34a levels were 5.8 ± 2.8 and 1.42 ± 0.85 (a.u.), respectively, while Ki-67 and p53 averaged 136.9 ± 79.9 and 93.15 ± 49.5 H-score points. Positive correlations were found between miR-21 and Ki-67 (r = 0.76) and miR-34a and p53 expressions (r = 0.65). Tumors with low Ki-67 showed 2.48-fold lower miR-21 levels, and low p53 tumors showed 4.2-fold lower miR-34a levels. While no correlation with age or menstrual status was found, miR-21 (r = 0.78), Ki-67 (r = 0.68), and miR-34a (r = -0.59) correlated with CIN grading (p < 0.05). The miR-21 and Ki-67 levels increased in CIN 2 and CIN 3 compared to CIN 1 in both HPV-positive and HPV-negative samples. The miR-34a levels were the lowest in CIN 3 HPV-negative samples and significantly decreased with CIN progression in HPV-positive samples. The p53 levels were significantly higher in CIN 3 cases of both the HPV-positive and HPV-negative groups.</p><p><strong>Conclusion: </strong>Our study demonstrates that the miR‑21, miR-34a, Ki-67, and p53 expression levels are significantly correlated with each other and are distinctly associated with the progression of CIN grades and HPV status, highlighting their potential as crucial CC biomarkers.</p>","PeriodicalId":94318,"journal":{"name":"Experimental oncology","volume":"47 2","pages":"223-229"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ALPHA-TOCOPHEROL AND G-CSF CHANGE EXPRESSION OF GENES ASSOCIATED WITH DIFFERENTIATION OF K562 CHRONIC MYELOID LEUKEMIA CELLS DOWNREGULATING EMT-ASSOCIATED STEMNESS BIOMARKERS.","authors":"L Shvachko, M Zavelevich, M Dybkov, I Gartovska, G Telegeev","doi":"10.15407/exp-oncology.2025.02.181","DOIUrl":"https://doi.org/10.15407/exp-oncology.2025.02.181","url":null,"abstract":"<p><strong>Background: </strong>Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder characterized by a block of myeloid differentiation, finally resulting in the uncontrolled expansion of CML stem cells in a phase of blast crisis of the disease. Tyrosine kinase inhibitors (TKI) are effective in delaying CML progression for a long time. Nevertheless, CML cells become resistant to TKI over time. Therefore, the search for alternative and complementary therapies, including differentiation therapy, is currently in the limelight. The aim of the study was to explore the differentiation potential of alpha-tocopherol and granulocyte-colony stimulating factor (G-CSF) by analyzing the gene expression of several factors critical for myeloid differentiation of K562 CML cells, as well as some key leukemic stemness transcription factors.</p><p><strong>Materials and methods: </strong>The mRNA expression of C/EBPα (CCAAT/enhancer binding protein alpha), neutrophil-granulocytic factor TNAP (tissue non-specific alkaline phosphatase), E-cadherin, SNAIL, OCT4, and PLAP (placental-like alkaline phosphatase) was studied by qRT-PCR in K562 cells exposed to alpha-tocopherol or G-CSF.</p><p><strong>Results: </strong>K562 cell exposure to alpha-tocopherol or G-CSF resulted in the CEBPB, CDH1, and ALPL gene upregulation. At the same time, down-regulation of EMT-associated markers SNAIL, PLAP, and OCT4 (SNAI1, ALPP, and POU5F1 genes) was demonstrated.</p><p><strong>Conclusion: </strong>The inverse relationship between expression of the genes of leukemic stemness cell markers SNAIL, OCT4, and PLAP and the genes of myeloid differentiation markers C/EBPα, TNAP, and E-cadherin in K562 cells exposed to alpha-tocopherol or G-CSF suggests the activation of the molecular pattern of myeloid differentiation in this setting.</p>","PeriodicalId":94318,"journal":{"name":"Experimental oncology","volume":"47 2","pages":"181-187"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CLINICO-MORPHOLOGICAL FEATURES OF THE LUMINAL B SUBTYPE OF BREAST CANCER IN YOUNG WOMEN.","authors":"O Martyniuk, I Smolanka, I Smolanka, V Med, T Tarasenko, V Chekhun","doi":"10.15407/exp-oncology.2025.02.245","DOIUrl":"https://doi.org/10.15407/exp-oncology.2025.02.245","url":null,"abstract":"<p><strong>Aim: </strong>To evaluate the clinical and morphological features of breast cancer (BC) of the luminal B subtype in young women to determine biological aggressiveness, response to neoadjuvant chemotherapy, and prognosis.</p><p><strong>Materials and methods: </strong>A retrospective study included luminal B subtype BC patients under 40 years of age (n = 108) and over 55 years (n = 101) treated at the National Cancer Institute. All patients received neoadjuvant chemotherapy according to the ddAC-12T regimen. TNM stages, tumor differentiation grade, Ki-67 expression, hormone receptor status, response to the neoadjuvant chemotherapy (NAC) (RECIST 1.1), pathomorphology grade (Miller - Payne), disease-free (DFS) and overall survival (OS) were analyzed.</p><p><strong>Results: </strong>Patients of the younger age group were more likely to have G3 tumors (68% vs. 45%), high Ki-67 levels >35% (72% vs. 50%), and lymph node involvement (71% vs. 59%). The median estrogen receptor expression was 35% in the young patients vs. 65% in the older patients. Complete histological response to NAC was achieved in 26% of the young patients (vs. 9% in the older group). Five-year RFS in the young women was 82.4% vs. 94.1% in the older group.</p><p><strong>Conclusions: </strong>The luminal B subtype of BC in the young women is characterized by the higher proliferative activity, lower hormonal sensitivity, and more frequent lymph node involvement. Despite the response to NAC, this group demonstrates the worse DFS. The results confirm the need for personalized treatment strategies and improved early diagnosis programs in young patients.</p>","PeriodicalId":94318,"journal":{"name":"Experimental oncology","volume":"47 2","pages":"245-250"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ILLUSTRATIVE CASE OF CAPILLARY HEMANGIOMA OF THE OPTIC NERVE.","authors":"O Danevych, A Mumliev, A Lisianyi, M Solovey, K Iegorova, M Guk, O Ukrainets, A Chukov, O Balimchuk, O Ostrovskyi, Ie Iarmoliuk","doi":"10.15407/exp-oncology.2025.02.251","DOIUrl":"https://doi.org/10.15407/exp-oncology.2025.02.251","url":null,"abstract":"<p><p>Capillary hemangiomas (CH) are benign proliferative vascular neoplasms commonly present in skin and soft tissues but rarely found intracranially. We describe a case of a 14-year-old male patient with histologically proven CH of the optic nerve, who underwent surgical resection of the lesion due to progressive visual loss. Imaging studies revealed a cystic-solid formation in the suprasellar region lateralized to the right and located above the pituitary gland. This case shows that CH may originate from the optic nerve, leading to its gradual compression and causing optic neuropathy. While the correct differential diagnosis on the MRI may be difficult, surgical treatment is warranted in cases of progressive visual decline.</p>","PeriodicalId":94318,"journal":{"name":"Experimental oncology","volume":"47 2","pages":"251-259"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATING PERFORMANCE OF COMBINED HEMOGLOBIN AND TRANSFERRIN DETECTION IN FECAL IMMUNOCHEMICAL TESTING FOR COLORECTAL NEOPLASIA.","authors":"Ida Parwati, Ronal Winter, Anna Tjandrawati, Delita Prihatni, Didik Setyo Heriyanto, Anton Sumarpo","doi":"10.15407/exp-oncology.2025.02.238","DOIUrl":"https://doi.org/10.15407/exp-oncology.2025.02.238","url":null,"abstract":"<p><strong>Background: </strong>Evidence-based screening strategies can substantially reduce colorectal cancer (CRC) mortality. While colonoscopy is the gold standard, its invasiveness renders it less preferable as an initial screening tool. A two-step approach using a non-invasive fecal immunochemical test (FIT) followed by a confirmatory colonoscopy is gaining favor. A novel FIT that simultaneously detects fecal hemoglobin (F-Hb) and fecal transferrin (F-Tf) demonstrates variable diagnostic performance.</p><p><strong>Aim: </strong>This study compared the diagnostic performance of four screening strategies using three FITs with different cutoffs for F-Hb and F-Tf to detect neoplastic lesions in patients with suspected CRC.</p><p><strong>Materials and methods: </strong>We conducted a cross-sectional study involving suspected CRC patients aged ≥ 18 at Hasan Sadikin Hospital, Bandung, from March 2023 to August 2023. The study included 72 clinically suspected CRC patients who underwent colonoscopy. We compared four CRC screening strategies using FITs designated as FIT-I (F-Hb ≥ 10 ng/mL), FIT-II (F-Hb ≥ 50 ng/mL), FIT-IIIa (F-Hb ≥ ≥ 100 ng/mL or F-Tf ≥ 40 ng/mL), and FIT-IIIb (F-Hb ≥ 100 ng/mL and F-Tf ≥ 40 ng/mL).</p><p><strong>Results: </strong>The FIT-IIIb strategy, which requires positive results for both markers, yielded the highest diagnostic performance for detecting neoplastic lesions, with 60.0% sensitivity, 96.6% specificity, a 93.8% positive predictive value, and a 73.7% negative predictive value.</p><p><strong>Conclusion: </strong>A dual-marker FIT detecting both F-Hb and F-Tf is a promising and effective screening tool for CRC. Future research should explore its implementation in broader populations and potential impacts on screening guidelines.</p>","PeriodicalId":94318,"journal":{"name":"Experimental oncology","volume":"47 2","pages":"238-244"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PREVENTION OF PACLITAXEL-INDUCED MOTOR NEUROPATHY OF FIBULAR AND TIBIAL NERVES WITH ALPHA-LIPOIC ACID AND IPIDACRIN HYDROCHLORIDE IN BREAST CANCER PATIENTS.","authors":"I Holotiuk, A Kryzhanivska, S Holotiuk, T Teren, H Hirna","doi":"10.15407/exp-oncology.2025.02.230","DOIUrl":"https://doi.org/10.15407/exp-oncology.2025.02.230","url":null,"abstract":"<p><strong>Aim: </strong>To investigate neurofunctional parameters of motor nerves in breast cancer (BCa) patients with paclitaxelinduced peripheral neuropathy (PIPN) and to determine the feasibility of using alpha-lipoic acid (ALA) in combination with ipidacrine hydrochloride (IPD) for PIPN prevention.</p><p><strong>Materials and methods: </strong>The study included 100 patients with BCa stages II-IV, who were treated with polychemotherapy (PCT) according to the AT (paclitaxel, doxorubicin) or ET (paclitaxel, epirubicin) scheme in the neoadjuvant, adjuvant, or palliative regimens. Patients were randomized into two groups (n = 50 in each): group I received PCT only; group II - PCT in combination with ALA + IPD. Electroneuromyographic (ENMG) studies of the motor fibular and tibial nerves were performed before the start of chemotherapy and after the 3rd and 6th cycles of PCT.</p><p><strong>Results: </strong>Comparison of ENMG parameters of the motor nerves of the lower extremities of BCa patients before the start of PCT with these parameters after 3 and 6 PCT cycles indicated a slightly pronounced but significant decrease in the M-response and partly the nerve conduction velocity, which progressed with an increase in the cumulative dose of paclitaxel. Despite this, the average values of ENMG parameters remained within normal limits even after 6 cycles of PCT. The detected changes indicated a tendency toward axonal damage and mild myelinopathy. Significantly higher M-response rates of motor nerves were found in patients of group II compared to group I only after 6 cycles of PCT with paclitaxel.</p><p><strong>Conclusion: </strong>The use of ALA and IPD improves the functional state of the axons in patients with BCa treated with paclitaxel.</p>","PeriodicalId":94318,"journal":{"name":"Experimental oncology","volume":"47 2","pages":"230-237"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COMPARATIVE STUDY OF SODIUM OXAMATE AND METFORMIN CYTOTOXICITY AGAINST LEWIS LUNG CARCINOMA CELLS UNDER ANCHORAGE-INDEPENDENT GROWTH.","authors":"D Kolesnik, Y Stepanov, I Prokhorova, Y Yakshibaeva, M Soldatkina, G Solyanik","doi":"10.15407/exp-oncology.2025.02.188","DOIUrl":"https://doi.org/10.15407/exp-oncology.2025.02.188","url":null,"abstract":"<p><strong>Background: </strong>The effect of the inhibitors of glycolysis and oxidative phosphorylation on the altered metabolism of neoplasms is considered a promising method of antitumor therapy. However, most studies on the antimetastatic activity of such inhibitors focus on analyzing their effect on the migratory and invasive characteristics of cells. Meanwhile, the survival of circulating metastatic cells and their resistance to anoikis are critically important factors in metastasis.</p><p><strong>Aim: </strong>To carry out a comparative study of sodium oxamate (SOX) and metformin (MTF) effects on the survival, proliferative activity, and metabolic plasticity of the low-metastatic variant of Lewis lung carcinoma (LLC/R9) cells under their anchorage-independent growth.</p><p><strong>Materials and methods: </strong>Cell death, apoptosis, cell cycle distribution, reactive oxygen species (ROS) production, glucose and lactate levels, and vimentin expression in LLC/R9 cells under their anchorage- independent growth were assessed following SOX and MTF treatments.</p><p><strong>Results: </strong>The cytotoxicity of inhibitors was manifested in a significant decrease in the number of viable LLC/R9 cells and an increase in the number of dead and apoptotic cells, the effects being more pronounced for MTF. In the case of SOX treatment, a correlation was observed between an increase in the percentage of apoptotic cells and ROS level and a decrease in the glucose consumption rate (GCR). MTF increased GCR and the number of apoptotic cells, without changes in ROS levels. Incubation with MTF resulted in a significant twofold increase in the percentage of cells in the S phase due to a decrease in the fraction of cells in the G1/G0 and G2/M phases of the cell cycle.</p><p><strong>Conclusions: </strong>Unlike SOX, the cytotoxic effect of MTF on de-adhesive cells was directly related to disrupting energy homeostasis and cell cycle regulation rather than by oxidative stress. Their combined application could potentially reinforce metabolic stress in circulating tumor cells, simultaneously weakening glycolytic and oxidative compensatory pathways, thereby limiting metastatic competence.</p>","PeriodicalId":94318,"journal":{"name":"Experimental oncology","volume":"47 2","pages":"188-196"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}