Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine最新文献

{"title":"Clinical input of anti-D (anti-RH1) microtitration in the management of D negative (RH:-1) pregnant women. Experience of the French National Reference Center For Perinatal Hemobiology (CNRHP).","authors":"Marion Coux, Jenny Beaud, Cecile Toly-Ndour, Vincent Thonier, Stephanie Huguet-Jacquot, Elodie Maenulein, Michel Vaubourdolle, Agnès Mailloux","doi":"10.1016/j.tracli.2026.07.002","DOIUrl":"10.1016/j.tracli.2026.07.002","url":null,"abstract":"<p><strong>Background and objectives: </strong>The administration of anti-D immunoglobulin (RhIg) has significantly reduced the incidence of anti-D alloimmunization. However, it complicates the interpretation of antibody screening, as it is necessary to distinguish passive anti-D from early alloimmunization. Microtitration is the reference method for making this distinction. The aim of this study was to evaluate the impact of anti-D microtitration on the immuno-hematological and clinical management of RH:-1 (D-) pregnant women in the CNRHP in 2024.</p><p><strong>Materials and methods: </strong>This retrospective observational study included all anti-D microtitrations performed between January 1st and December 31st, 2024 in the CNRHP. The anti-D microtitration impact was analyzed in three settings: antenatal care, delivery, and fetomaternal hemorrhage (FMH).</p><p><strong>Results: </strong>A total of 3281 microtitrations were performed. Antenatally, 84.6% of results matched the expected anti-D levels, while 10.5% were higher, leading to 11 diagnoses of alloimmunization. At delivery, 603 microtitrations identified two new alloimmunizations and prevented RhIg injections in 30 patients. In FMH setting 11.3% of the cases required multiple doses, with microtitration being essential for assessing neutralization. This was especially observed in massive FMH.</p><p><strong>Conclusion: </strong>The CNRHP's experience highlights that anti-D microtitration is an essential tool for distinguishing passive from immune anti-D, enabling early detection of alloimmunization, avoiding unnecessary monitoring and RhIg injections, and optimizing dose adjustment in cases of FMH.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148498621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From active surveillance to precision donor haemovigilance: The next step in plateletpheresis donor safety.","authors":"Rishiraj Sinha, Devesh C Dubey","doi":"10.1016/j.tracli.2026.07.006","DOIUrl":"10.1016/j.tracli.2026.07.006","url":null,"abstract":"","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148458264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond myths: Transfusion literacy as the next frontier in patient-centred transfusion medicine.","authors":"Devanshi Khare, Rishiraj Sinha","doi":"10.1016/j.tracli.2026.07.007","DOIUrl":"10.1016/j.tracli.2026.07.007","url":null,"abstract":"","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148458233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of freezing methodology on coagulation factor preservation in fresh frozen plasma: A pilot study.","authors":"Amit Ajay Pawar, Akshaya Tomar, Anoop Sharma, Ushasree Sadana, Neerja Kushwaha","doi":"10.1016/j.tracli.2026.07.004","DOIUrl":"10.1016/j.tracli.2026.07.004","url":null,"abstract":"<p><strong>Background and objectives: </strong>Rapid freezing is thought to better preserve labile coagulation factors in fresh frozen plasma (FFP). This pilot study compared contact shock freezing (CSF) with conventional freezing (CF) to assess their effectiveness in maintaining post-thaw coagulation factor levels. Although the ABO blood group may influence plasma characteristics, its role was not a primary objective and was only noted for preliminary observation during the comparison of the two freezing methods.</p><p><strong>Materials and methods: </strong>In a prospective pilot study, plasma from 75 donors (balanced by ABO group) was divided into matched aliquots and subjected to either CSF (30min at -30°C) or CF (7-8hours at -30°C). Post-thaw analyses of PT, aPTT, fibrinogen, factor V, factor VIII, and factor IX were performed. Non-parametric statistics (Wilcoxon Signed-Rank tests and Aligned Ranks Transform ANOVA) were used for analysis.</p><p><strong>Results: </strong>Study data suggested a trend towards superior preservation with CSF, yielding lower median PT and aPTT and higher median activities of fibrinogen, factor V, and factor VIII. Notably, statistically significant interaction effects between the freezing method and ABO group were observed for fibrinogen (P=0.033), factor VIII (P=0.012), and factor IX (P=0.003). Post-hoc analysis indicated that the potential benefit of CSF for fibrinogen may be most pronounced in group AB, and for factor VIII in group B, while CF might better preserve factor IX in group A.</p><p><strong>Conclusion: </strong>This in vitro pilot study demonstrates that contact shock freezing is superior to conventional freezing in preserving the coagulation integrity of fresh frozen plasma across all major parameters. The observed ABO-related differences emerged as incidental exploratory findings and should be interpreted with caution. The clinical significance of these biochemical improvements requires further investigation in well-designed clinical outcome studies.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148458225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating laboratory-verified ABO and RhD blood group information into India's Aadhaar card: A public health perspective.","authors":"Neema Vijay, Manish Raturi, Parag Prabhakar Fulzele, Juilee Shalik Charmode, Soumya Das, Rounak Dubey","doi":"10.1016/j.tracli.2026.07.003","DOIUrl":"10.1016/j.tracli.2026.07.003","url":null,"abstract":"","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequent overactivation of massive transfusion protocol: An underrecognized challenge for blood resource stewardship.","authors":"Rajat Bansal, Neerav Saini","doi":"10.1016/j.tracli.2026.07.001","DOIUrl":"10.1016/j.tracli.2026.07.001","url":null,"abstract":"","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148406951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Albumin priming enables safe and effective red blood cell exchange in low-weight children with sickle cell disease.","authors":"O Hequet, D Revesz, Y Chelghoum, K Kebaili, A L Peugnet, A Villars, C Baud, F Cognasse, A Guironnet-Paquet, C Halfon-Domenech, P Joly, P Connes","doi":"10.1016/j.tracli.2026.06.011","DOIUrl":"10.1016/j.tracli.2026.06.011","url":null,"abstract":"<p><p>Red blood cell (RBC) exchanges (RCE) are particularly efficient to treat and prevent complications in patients with sickle cell anemia (SCA). However, the low-weight of young children is a barrier to their use. Spectra Optia is a therapeutic apheresis platform that enables automatic priming to prevent hemodynamic complications in low-weight children. The easiest method is to use albumin for priming, which is allowed by the software. We conducted a retrospective monocenter analysis of the feasibility, safety, and efficacy of albumin priming during RCE (RCE/Alb-primed) in 19 children weighing 12 to 23kg for a total of 153 sessions. They were treated either on an emergency basis (8 children, 8 sessions), before surgery (2 children, 4 sessions) or as part of a RCE program (9 children, 141 sessions). Indications of RCE/Alb-primed sessions included: (i) an extracorporeal volume of the kit equal to 15% of the child's total blood volume; (ii) a weight of less than 20kg; or (iii) a weight between 20 and 25kg with a hematocrit (Hct) of 20% or less. We monitored Grade 2 adverse events (AEs) during the sessions, in extenso decreased blood pressure, increased heart rate and fatigue. Post-apheresis hematocrit and the fraction of cell remaining (FCR) values after RCE were compared to the expected ones. A low incidence of complications was observed in the 153 RCE/Alb-primed sessions with only 1 episodes of transient Grade 2 AEs. Post-apheresis Hct and FCR reached expected values with the RCE/Alb-primed method. In conclusion, RCE/Alb-primed seems to be safe and efficient in young SCA children and should be proposed systematically for curative RCE or prophylactic programs.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148383474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catastrophic CD34+ autologous hematopoietic stem cell collection despite sustained mobilization and solutions to rescue the harvests.","authors":"Laurence Raoult-Valette, Arnaud Campidelli, Isabelle Boutin, Isabelle Gauvrit, Florent Perdriel-Vaissière, Dehbia Menouche, Pauline Soler, Quoc Hung Le, Philippe Rey, Caroline Regny, Kahina Ouis Tahri, Nabih Azar, Angela Granata, Christina Vieira Dos Santos, Emmanuel Rouger, Aude Le Tallec, Olivier Hequet","doi":"10.1016/j.tracli.2026.06.010","DOIUrl":"10.1016/j.tracli.2026.06.010","url":null,"abstract":"<p><p>Poor collection of hematopoietic stem cells during insufficient mobilization for autologous stem cell transplantation has been widely described. However, only isolated cases of insufficient stem cell collection despite adequate mobilization prior to autologous transplantation have been reported. Over a 5-year period, French apheresis teams reported 31 cases of highly disastrous HSC collection (1.0±0.9 × 10⁶ CD34+ cells/kg, median 0.65) (using a continuous collection method), despite adequate levels of circulating CD34+ cells (79 ± 49/μL, median 79/μL) during the processing of 3 blood volumes. Collection efficiency is the main indicator for assessing the ability to collect cells during cytapheresis. These collections were characterized by disastrous collection efficiencies (CE₂), considered very low below 20% (8/31 patients) and catastrophic below 10% (23/31 patients). The teams attempted to find solutions to compensate for these disastrous and unexpected collections. Changes to the collection parameters led only to minor improvements in collection efficiency (CE₂=8 ± 5 vs 5 ± 2%) and the amount of hematopoietic stem cells collected (1.46 ± 1.39 vs 0.56 ± 0.45 × 10⁶ CD34+ cells/kg), while switching from a continuous collection technique to a discontinuous technique resulted in a significant improvement in collection efficiencies (39 ± 20 vs 8 ± 6%) and the amount of hematopoietic stem cells collected (4.64 ± 3.39 vs 1.09 ± 0.88 × 10⁶ CD34+ cells/kg). Our work highlights the importance of switching from a continuous collection technique to a discontinuous technique in cases of catastrophic collection despite appropriate hematopoietic stem cell mobilization.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrected count increment as a predictor of early graft dysfunction and mortality following living donor liver transplantation.","authors":"Deepti Sachan, Amal F Sam, Lavanya Sree B, Ashwin Rammohan, Deepthi Krishna G, Akila Rajakumar, Rajesh Rajalingam, Mohamed Rela","doi":"10.1016/j.tracli.2026.06.007","DOIUrl":"10.1016/j.tracli.2026.06.007","url":null,"abstract":"<p><strong>Background: </strong>Thrombocytopenia is prevalent in living donor liver transplantation (LDLT), resulting from underlying liver disease and perioperative factors. Although platelet transfusions are frequently required, their response is variable and may be associated with adverse outcomes. This study evaluated the prevalence and severity of thrombocytopenia, platelet transfusion practices, assessed post-transfusion response using corrected count increment (CCI), and predictors of poor CCI in adult LDLT recipients.</p><p><strong>Study design & methods: </strong>This prospective observational study was conducted at a quaternary care center after ethical committee approval. Adult LDLT recipients were enrolled, and clinical, biochemical, intraoperative, and transfusion data were collected. Platelet transfusion response was assessed using CCI post-transfusion; CCI <5,000 was considered a poor response.</p><p><strong>Results: </strong>Among 100 LDLT recipients (median age 52 years; 88% male), preoperative thrombocytopenia was observed in 91% of patients (17% severe). Intraoperative platelet transfusions were administered in 18% of cases. Postoperatively, 66 platelet transfusions were given to 23 patients, with a mean CCI of 9.0×10⁹/L. Poor CCI was observed in 10 patients and was associated with higher bilirubin levels, prolonged INR, longer ICU stay, and increased 30-day mortality. Early allograft dysfunction (EAD) at postoperative day 7 was an independent predictor of poor CCI (OR 10.2, 95% CI 2.4-43.1). An absolute platelet count ≤71,000/mm³ on day 7 predicted EAD with 85% sensitivity (AUC 0.79).</p><p><strong>Conclusions: </strong>Thrombocytopenia is highly prevalent in LDLT recipients. Poor CCI is associated with graft dysfunction and adverse outcomes, suggesting that CCI monitoring may help to prognosticate and anticipate worser outcomes.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TLR4/MD-2 complex promotes transfusion-related acute lung injury pathogenesis through the activation of the AP-1 and NF-κB signaling pathways.","authors":"Lingling Liu, Yi He, Min Chen, Caihan Zhao, Qing Tang, Yong Zou, Lin Chen, Ruifang Fan","doi":"10.1016/j.tracli.2026.06.009","DOIUrl":"10.1016/j.tracli.2026.06.009","url":null,"abstract":"<p><strong>Background: </strong>Transfusion-related acute lung injury (TRALI) is a severe complication of blood transfusion, but its molecular mechanisms remain poorly understood. This study aimed to investigate the role of Toll-like receptor 4 (TLR4) and its downstream signaling cascade in the pathogenesis of TRALI.</p><p><strong>Methods: </strong>Lipopolysaccharide (LPS)-treated PAEC cells and BALB/c mice were used as a model of TRALI. Relative mRNA expression was evaluated via quantitative RT-PCR. Protein abundance in cells/tissues and cell culture supernatant/serum was detected using western blot and ELISA, respectively. Lung tissue injury was evaluated by hematoxylin and eosin staining, and protein expression in lung tissues was analyzed by immunohistochemistry.</p><p><strong>Results: </strong>Expressions of TLR2, TLR4 and myeloid differentiation 2 (MD-2) were significantly elevated in the cellular TRALI model and accompanied by an increase in c-Jun, c-Fos, and P65 phosphorylation and increased expression and secretion of IL-1β, IL-6, IL-8, and TNF-α. The TLR4 inhibitor TAK-242 or MD-2 siRNAs effectively suppressed the molecular alterations induced by LPS in the cellular TRALI model. TAK-242 significantly reduced mortality and lung tissue injury in the TRALI mouse model, decreased TLR2, TLR4 and MD-2 expression, inhibited c-Jun, c-Fos, and p65 phosphorylation, and downregulated IL-1β, IL-6, IL-8, and TNF-α expression.</p><p><strong>Conclusions: </strong>In this animal model of TRALI, the highly expressed TLR4/MD-2 complex promotes the pathogenesis of TRALI through the activation of the activator protein-1(AP-1) and nuclear factor-κB (NF-κB) signaling pathways and the release of inflammatory mediators. One limitation is the positive study in rodent model but not in humans.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148321391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}