Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine最新文献

{"title":"Standardized Preparation and Characterization of Leukocyte-Rich and Leukocyte-Poor PRP Using PrecurCell® Kits.","authors":"Garima Singh, Sankarganesh Arunachalam, Vignesh Subramanian, Sridhar Haranahalli, Sayanti Pal, D U Vinay, Sankhadip Dolui, Monika Ramesh, Sumit Sahoo","doi":"10.1016/j.tracli.2026.10.003","DOIUrl":"https://doi.org/10.1016/j.tracli.2026.10.003","url":null,"abstract":"<p><strong>Objectives: </strong>To compare the cellular composition of leukocyte-rich platelet-rich plasma (LR-PRP) and leukocyte-poor platelet-rich plasma (LP-PRP) prepared using PrecurCell® PRP and PrecurCell®-LP PRP kits, with particular emphasis on platelet enrichment, leukocyte content, and residual red blood cells.</p><p><strong>Material and methods: </strong>Whole blood samples from healthy donors were processed using the two PRP preparation systems. Platelet concentration and red blood cell content were quantified using a hematology analyzer, and leukocyte content was assessed to characterize the resulting LR-PRP and LP-PRP preparations. Platelet enrichment was determined relative to baseline whole blood values.</p><p><strong>Results: </strong>The preparations achieved platelet concentrations up to six times the baseline whole blood values. Differences in leukocyte content distinguished the LR-PRP and LP-PRP preparations. No residual red blood cells were detected in the analyzed PRP samples.</p><p><strong>Conclusion: </strong>The evaluated systems produced platelet-enriched preparations with distinct leukocyte profiles and no detectable residual red blood cells. These findings characterize the cellular differences between LR-PRP and LP-PRP and may inform further investigation of their application-specific biological effects. Functional and clinical studies are required to establish their therapeutic relevance.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haemovigilance and Epidemiological Surveillance of Transfusion-Transmitted Infections in Greece, 1996-2023: National Donor Screening Data and Recipient Investigations.","authors":"C Politis, S Delicou, S Mellou, K Ventiadi, M Diamantidis, I Lafiatis, M Asariotou, E Grouzi, A Gafou, L Politi, K Stamoulis, V Bakaloudi, M Papé, E Zervou, G Martinis, M Hatzitaki, P Chalkia, P Damaskos, V Ladis, M Karagiorga, E Vrettou, L Kavalierou, G Bollas, E Nomikou, T Georgakopoulou, G Dougas, M Deutsch, J Koskinas, D Paraskevis, C Richardson","doi":"10.1016/j.tracli.2026.10.001","DOIUrl":"https://doi.org/10.1016/j.tracli.2026.10.001","url":null,"abstract":"<p><strong>Background: </strong>Surveillance of transfusion-transmitted infections (TTIs) supports blood safety, particularly for multi-transfused patients such as those with haemoglobinopathies.</p><p><strong>Materials and methods: </strong>Retrospective descriptive analysis of aggregated national surveillance data from Greece. We report aggregate infectious-marker positivity values from national surveillance summaries for 1996-2009. We also report NAT yields in 2007-2023, the transmission categories of HIV-positive donors in 2010-2019 and recipient investigations reported to the national haemovigilance system in 2005-2023.</p><p><strong>Results: </strong>More than 14 million donations were tested in 1996-2023. For 1996-2009, the surveillance summaries reported aggregate positivity of 0.39% for HBsAg, 0.09% for anti-HCV, 0.017% for syphilis and 0.008% for anti-HIV1/2. In 2007-2023, NAT detected 1,393 serology-negative infectious donations among 8,389,862 donations reported as tested (estimated 16.6 per 100,000; 95% CI 15.7-17.5), of which 1,321 were HBV. In 2010-2019, 56.9% of HIV-positive donors were men who have sex with men and 25.3% were heterosexual. Fifteen recipient investigations were reported among 13.6 million issued components; transmission was excluded in one.</p><p><strong>Conclusion: </strong>In 2007-2023, NAT detected infectious donations that serology missed, mostly HBV. Reported recipient infections were rare but included emerging pathogens. Annual data are needed to assess trends.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Error-based simulation to improve transfusion safety : Evaluation of «Transfusion Error Room ».","authors":"Taicir Rekik, Jalel Gargouri, Ikram Ben Amor","doi":"10.1016/j.tracli.2026.10.002","DOIUrl":"https://doi.org/10.1016/j.tracli.2026.10.002","url":null,"abstract":"<p><strong>Background and aim: </strong>Transfusion errors are mainly related to human factors and are largely preventable. We developed a simulation-based activity, the \"Transfusion Error Room,\" to describe its implementation and assess undergraduate students' ability to identify transfusion-related errors.</p><p><strong>Methods: </strong>The activity was conducted at the Simulation Center of the Medical School of Sfax among second-cycle medical students. A scenario containing nine transfusion-related errors, selected from local haemovigilance experience and reported adverse transfusion events, was designed and validated by faculty members with expertise in simulation-based education. Students identified the embedded errors and participated in a role-play activity, followed by a structured debriefing. A satisfaction survey was subsequently administered to the students.</p><p><strong>Results: </strong>A total of 244 students participated in 16 sessions and identified a mean of 4.62 of the nine embedded errors (range, 1-8). The least frequently identified errors were pre-labelled blood collection tubes, phenotype incompatibility in the presence of an anti-KEL1 antibody, and blood-component traceability. Learner satisfaction was high, with 98.77% reporting that they were satisfied or very satisfied. In addition, 97.54% reported perceived acquisition of useful knowledge.</p><p><strong>Conclusion: </strong>The \"Transfusion Error Room\" is an immersive and well-accepted simulation activity that provides a descriptive assessment of error detection and highlights inappropriate transfusion practices that may represent educational needs.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"National blood-service continuity during armed conflict: a 39-day operational analysis of collection, redistribution and red-cell inventory in Iran.","authors":"Nasim Sadat Hosseini Divkolaye, Gilda Esmaelifar, Babak Yektaparast, Shahram Shekarchi, Seyed Mohammad Shahzadeh Safavi, Ahmad Gharehbaghian","doi":"10.1016/j.tracli.2026.09.009","DOIUrl":"https://doi.org/10.1016/j.tracli.2026.09.009","url":null,"abstract":"<p><strong>Background: </strong>Armed conflict can disrupt blood collection, inventory management and distribution, placing continuity of essential transfusion services at risk.</p><p><strong>Objective: </strong>To describe national blood-service operations and response measures during 39 days of armed conflict in Iran.</p><p><strong>Methods: </strong>This retrospective comparative operational study used aggregated routine data from the Iranian Blood Transfusion Organization (IBTO) for 28 February-7 April 2026 and the corresponding 39-day period in 2025. Outcomes were donor presentations, whole-blood collections, first-time and female donors, interprovincial red blood cell (RBC) redistribution and national RBC inventory in days of supply.</p><p><strong>Results: </strong>Donor presentations increased from 262 403 to 301 006 (14.7%) and successful whole-blood collections from 220 361 to 247 893 (12.5%). First-time donors increased by 63.9%, female donors by 79.6%, and interprovincial RBC redistribution by 97.9%. Mean national RBC inventory increased from 5.4 to 7.8 days of supply; it exceeded the 7-day operational reserve target on day 10 and remained above that target thereafter. In the supplementary operational summary, recorded expiry-to-production proportions were 0.032% for RBC, 0.089% for LRBC, 4.90% for platelets and 0.051% for FFP. Post-conflict donation counts varied by month compared with 2025.</p><p><strong>Conclusion: </strong>During the conflict, IBTO sustained collection and redistribution activities while national RBC inventory increased above its operational reserve target. Available supplementary data describe recorded expiry but do not measure total wastage; aggregate post-conflict counts cannot establish the effect of donor deferral or clinical consequences.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149121358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reassessing cytomegalovirus as a relevant transfusion-transmitted infection.","authors":"Jeremy W Jacobs, Steven J Drews, Evan M Bloch","doi":"10.1016/j.tracli.2026.09.007","DOIUrl":"10.1016/j.tracli.2026.09.007","url":null,"abstract":"","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-transfusion purpura: Pathogenic mechanisms and relationship with immune thrombocytopenia: A critical analytical review.","authors":"Tian-Cheng Shen, Feng-Qing Wang, Ming Gao","doi":"10.1016/j.tracli.2026.09.008","DOIUrl":"10.1016/j.tracli.2026.09.008","url":null,"abstract":"<p><p>Post-transfusion purpura (PTP) is a rare, life-threatening immune-mediated adverse transfusion event. It is characterized by severe thrombocytopenia developing 5-10 days after transfusion of platelet-containing blood products. The most notable pathological paradox is that alloantibodies directed against foreign human platelet antigen (HPA) can destroy the patient's autologous platelets that do not express the corresponding target antigen. PTP predominantly affects middle-aged women, and its major clinical manifestation is mucocutaneous bleeding, with intracranial hemorrhage being the leading cause of death. The disorder is mostly self-limiting, and three major hypotheses have been proposed to explain its pathogenesis: immune-complex-mediated injury, antigen adsorption, and concomitant autoantibody production. Evidence from clinical serological assays, recombinant autoantibody studies and animal models suggests that B-cell somatic hypermutation drives the transition from alloimmunity to transient autoimmunity with the generation of anti-platelet autoantibodies, which constitutes the core pathogenic mechanism of PTP. By contrast, the immune-complex pathway and antigen-adsorption mechanism likely exert auxiliary and synergistic effects, without sufficient in-vivo human evidence to validate them. PTP shares certain similarities while exhibiting marked distinctions from immune thrombocytopenia (ITP). Pathogenic antibodies in both conditions target conserved epitopes on platelet glycoproteins. Nevertheless, PTP is initiated by memory-B-cell-mediated anamnestic alloimmunity, with transient autoantibodies and mostly spontaneous clinical resolution. ITP, by comparison, represents a chronic primary autoimmune disorder with persistently circulating autoantibodies that frequently require long-term immunosuppressive therapy. Single-cell multi-omics technologies hold promise for deciphering the clonal relationship between alloantibody-secreting and autoantibody-secreting B cells, elucidating the transient autoimmune cascade underlying PTP, and offering novel therapeutic targets for dissecting immune tolerance breakdown and treating refractory antibody-mediated autoimmune hematological diseases including ITP.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structured donor re-engagement following temporary deferral: A prospective implementation study of donor re-entry.","authors":"Rajat Bansal, Neerav Saini","doi":"10.1016/j.tracli.2026.09.006","DOIUrl":"10.1016/j.tracli.2026.09.006","url":null,"abstract":"<p><strong>Background: </strong>Temporary blood donor deferral may interrupt subsequent donor participation. Structured follow-up may provide an opportunity for donor re-engagement and reassessment of eligibility. This study evaluated the implementation and donor re-entry outcomes of a structured donor re-engagement programme incorporating cause-specific recall and telephonic follow-up.</p><p><strong>Methods: </strong>A prospective implementation study was conducted from January to June 2026 at a tertiary care teaching hospital blood centre in India. Temporarily deferred donors who consented to follow-up received cause-specific counselling, scheduled telephone reassessment, and targeted interventions where appropriate. The primary outcome was successful donor re-entry, defined as whole-blood donation at the study blood centre following temporary deferral and programme enrolment. Donations reported at other centres were recorded separately.</p><p><strong>Results: </strong>Among 2345 donors screened, 1,760 (75.1%) donated, 518 (22.1%) were temporarily deferred, and 67 (2.9%) permanently deferred. Of the temporarily deferred donors, 451 (87.1%) enrolled in the programme. Successful re-entry occurred in 223/451 (49.4%). Re-entry varied by deferral category: inadequate sleep, 71/88 (80.7%); elevated blood pressure, 21/34 (61.8%); low haemoglobin in males, 32/60 (53.3%); antibiotic therapy, 19/43 (44.2%); menstruation, 26/62 (41.9%); poor venous access, 17/42 (40.5%); and low haemoglobin in females, 37/122 (30.3%). A further 16/451 (3.5%) reported donation elsewhere.</p><p><strong>Conclusion: </strong>The structured donor re-engagement pathway was feasible to integrate into routine blood-centre practice. Approximately half of enrolled temporarily deferred donors subsequently donated at the study centre. Given the absence of a comparator and limited long-term follow-up, these findings represent donor re-entry outcomes rather than evidence of a causal effect on retention.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149066989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge management in computerised systems validation at GMP facilities: A qualitative interview study with specialists in the Austrian public health sector.","authors":"Thomas Bernsteiner, Anita Siller","doi":"10.1016/j.tracli.2026.09.005","DOIUrl":"10.1016/j.tracli.2026.09.005","url":null,"abstract":"<p><strong>Background: </strong>Working methods grounded in knowledge management add structure and recurring value creation to organisational processes. For GMP facilities, knowledge management (KM) around the validation of computerised systems could unlock existing knowledge potential and reduce dependence on external consultants, yet the topic is barely addressed in the scientific literature.</p><p><strong>Objective: </strong>To determine which aspects of knowledge management specialists consider suitable for the application and validation of computerised systems, and which aspects have been used since their implementation.</p><p><strong>Methods: </strong>An exploratory qualitative design was applied. Nine semi-structured, guideline-based specialist interviews were conducted in Austrian public-sector GMP pharmacies and tissue banks. Interviews were subsequently analysed by structured qualitative content analysis, using Probst et al. as the deductive framework and inductively derived subcategories.</p><p><strong>Results: </strong>Statements were coded across all eight building blocks, with the densest coding in knowledge distribution. Documentation, conferences, continuing education and specialist literature formed the backbone of knowledge acquisition. Leadership behaviour, a blame-free culture and flat hierarchies were the decisive enablers of transfer of knowledge. Staff turnover was the principal source of knowledge loss. Knowledge-management aspects were known and partly implemented in every facility, but explicit knowledge goals and their assessment were largely absent.</p><p><strong>Conclusions: </strong>Knowledge management for CSV is operationalised only to a low degree in the investigated facilities, mainly because of limited public-sector resources, the difficult quantification of benefits and the absence of a holistic organisational commitment. Five recommendations for action are proposed as an empirical concretisation of known methods enriched by newly identified aspects.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149043093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rethinking platelet hemovigilance after CAR-T therapy.","authors":"Rishiraj Sinha, Satish Kumar","doi":"10.1016/j.tracli.2026.09.003","DOIUrl":"10.1016/j.tracli.2026.09.003","url":null,"abstract":"","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pattern of blood donor deferral and donor return following structured telephonic follow-up in a tertiary care blood centre in India.","authors":"Pandeep Kaur, Anuneet Tripathi, Ankita Nigam, Tarun Dhawan","doi":"10.1016/j.tracli.2026.09.002","DOIUrl":"10.1016/j.tracli.2026.09.002","url":null,"abstract":"<p><strong>Objectives: </strong>Blood donor deferral is an essential component of donor selection aimed at ensuring the safety of both donors and recipients. However, temporary deferral may negatively affect subsequent donor return and contribute to loss of potentially eligible donors. The present study aimed to evaluate the pattern of blood donor deferral and assess donor return following structured telephonic follow-up after temporary deferral in a tertiary care blood centre.</p><p><strong>Methods: </strong>This prospective observational study was conducted in the Department of Immunohematology and Blood Transfusion at National Institute of Medical Sciences and Research, Jaipur, Rajasthan, India. Donor recruitment was conducted from January 2026 to June 2026. All 339 temporarily deferred donors identified during the recruitment period constituted the follow-up cohort. Donor demographic details, type of donation, and causes of deferral were recorded. Temporarily deferred donors were contacted telephonically according to the eligibility timeline applicable to their individual deferral reason, using a standardized counselling script, and subsequent return for donation was assessed during the available observation period.</p><p><strong>Results: </strong>Among 4773 screened donors, 344 donors were deferred, resulting in an overall deferral rate of 7.2%. Temporary deferrals accounted for 339 (98.5%) of all deferrals. Low hemoglobin was the most common cause of deferral (30.7%), followed by recent antibiotic intake (14.5%), recent surgery (6.7%), poor venous access (5.6%), and tattooing (5.3%). Of the 339 temporarily deferred donors, 336 (99.1%) were successfully contacted, while 3 (0.9%) remained unreachable despite three attempts. Among the 336 successfully contacted donors, 144 (42.9%; 95% CI, 37.7-48.2%) returned to the blood centre and subsequently donated blood, whereas 192 (57.1%) did not return during the available observation period.</p><p><strong>Conclusions: </strong>Temporary donor deferral represents an important challenge to donor retention. Low hemoglobin was the leading cause of donor deferral in the present study. Structured telephonic follow-up was feasible and was associated with a 42.9% donor return rate among successfully contacted temporarily deferred donors. However, because the study did not include a non-intervention comparison group, the independent effect of telephonic follow-up on donor return cannot be established.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148992699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}