Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine最新文献

{"title":"Profiles of differential expression of miRNAs in the late stage of red blood cell preservation and their potential roles.","authors":"Yajie Wang, Yiming Ma, Liping Sun, Quan Rao, Xiaozhou Yuan, Yan Chen, Xiaofei Li","doi":"10.1016/j.tracli.2024.09.003","DOIUrl":"https://doi.org/10.1016/j.tracli.2024.09.003","url":null,"abstract":"<p><strong>Objective: </strong>To detect the differentially expressed regulatory miRNAs in the late stage of red blood cell (RBC) preservation and predict their roles.</p><p><strong>Methods: </strong>Suspended RBCs with different storage periods of 35 day, 42 day, and 50 day were collected for routine blood tests, RNA extraction, and preparation of small RNA sequencing libraries. The constructed libraries were sequenced and the biological functions of differential miRNAs in RBCs in the late storage were analyzed by bioinformatics.</p><p><strong>Results: </strong>Routine indicators of RBCs in the late stage were not significantly affected by preservation time. The Pearson correlation analysis performing on RBC miRNAs with different storage days revealed that RBC miRNAs changed with the increase of storage days. RBC miRNAs from day 35 (D35), day 42 (D42) and day 50 (D50) showed significant differences (P<0.05). Compared RBC miRNAs from D42 with these from D35, there were 690 up-regulated miRNAs and 82 down-regulated miRNAs; compared RBC miRNAs from D50 with these from D35, there were 638 up-regulated miRNAs and 123 down-regulated miRNAs; compared RBC miRNAs from D42 with these from D50, there were 271 up-regulated miRNAs and 515 down-regulated miRNAs. GO enrichment analysis of target genes of differential miRNAs were mainly involved in cell metabolism, biosynthesis, protein modification, gene expression and transcriptional regulation of biological processes. KEGG pathway enrichment analysis of miRNA target genes showed that differential miRNA target genes were closely related to pathways in cancer.</p><p><strong>Conclusion: </strong>MiRNAs were differentially expressed in the late stage of RBC preservation, and may be involved in various biological processes, especially cancer.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune cell kinetics after allogeneic red blood cell transfusion in patients undergoing cardiovascular surgery.","authors":"Marie Yamada, Mami Nakao, Naotomo Yamada, Hideaki Nakamura, Manabu Itoh, Junji Yunoki, Keiji Kamohara, Shinya Kimura, Eisaburo Sueoka","doi":"10.1016/j.tracli.2024.09.002","DOIUrl":"https://doi.org/10.1016/j.tracli.2024.09.002","url":null,"abstract":"<p><strong>Background and objectives: </strong>Recent reports have highlighted that allogeneic blood transfusions decrease immune responses and affect patient outcomes. However, the effects of allogeneic red blood cell transfusions on the composition of immune cells are unclear. We aimed to clarify the alterations in host immune cells in patients who received allogeneic red blood cell transfusions during the perioperative period of cardiovascular surgery.</p><p><strong>Materials and methods: </strong>Eight non-transfused, 22 intraoperative autotransfusions, and 36 allogeneic red blood cell-transfused patients undergoing surgery were grouped, and lymphocyte subsets were analyzed using flow cytometry. Blood samples collected before surgery, approximately 1-week, and 1-month after surgery were used for analysis. Surgical parameters, operation time, blood loss, and length of hospital stay were also assessed.</p><p><strong>Results: </strong>The group receiving transfusions showed statistical significance compared to non-transfused in the above-mentioned surgical parameters. When comparing the autologous and allogeneic transfusion groups, only the allogeneic red blood transfusion group had a longer hospital stay. In comparing preoperative and 1-week and 1-month postoperative samples, there were almost no differences in CD4, CD20, or NK counts between the autotransfusions and the allogenic red blood cell transfusion groups. In contrast, a significant decrease in lymphocyte count was observed in the allogenic red blood cell transfused group 1-week postoperatively compared to preoperatively. Moreover, the number of CD8+ cells was statistically lowest in the allogeneic transfusion group 1 week after the operation.</p><p><strong>Conclusion: </strong>Our results suggest that allogeneic red blood cell transfusion could alter immune cell composition especially CD8+ cells, potentially impacting immune function.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stratified analysis of risk factors affecting vasovagal reactions in first-time whole blood donors: a regional multi-center donor hemovigilance data study.","authors":"Jiangxiu Li, Junhong Yang, Qiuai Yang, Shengji Zhang, Hongbo Liu, Li Zhou, Xia Huang","doi":"10.1016/j.tracli.2024.09.001","DOIUrl":"https://doi.org/10.1016/j.tracli.2024.09.001","url":null,"abstract":"<p><strong>Background and objectives: </strong>Vasovagal reactions (VVR) are the most common adverse reactions in blood donation. This study aimed to provide and analyze the data of the regional hemovigilance system in Chongqing, China from 2020 to 2022, report the prevalence and explore the risk factors of VVR.</p><p><strong>Materials and methods: </strong>R software (version 4.2.3) was used for all statistical analyses. Frequency and composition rates were used to describe the data of total donation , as well as data on the different types of ADR. The Chi-square test was used to analyze risk factors for VVR and inter-group comparisons of VVR stratified by gender (female/male), age (18-22; 23-29; 30-39; 40-49; 50-60) and season(Spring (Mar.-May.); Summer (Jun.-Aug.); Autumn (sep.-Nov.); Winter (Dec.-Feb.)).</p><p><strong>Results: </strong>The reported incidence rate of VVR was 8.69‰ during whole blood donations and 1.02‰ during platelet-apheresis donations.The stratified analysis revealed that female donors aged 18-22 years old and 30-39 years old were reported to have lower VVR rates than male donors, oppositely, higher in females in 50-60 years old. Statistically significant differences in the incidence rate of VVR were observed between winter and summer, and between winter and spring in 18-49 years old. No seasonal variation was found in 50-60 years old.</p><p><strong>Conclusions: </strong>The reported incidence rate of VVR related to blood donation was very low and varied from those calculated by other haemovigilance systems. The higher prevalence of VVR in young, first-time donors, college students, donating in mobile vehicles, males and in spring. Among first-time donors, the prevalence of VVR was higher in males than in females; and stratified analysis revealed there were seasonal variation and gender differences within a same age group.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging the gaps: A prospective analysis of root causes for rejection and incompleteness in blood requisition forms.","authors":"Shweta Ranjan, Nishith Nayan, Bankim Das, Rakesh Kumar, Saurabh Lahare, Neha Singh, Ruchi Sinha","doi":"10.1016/j.tracli.2024.08.005","DOIUrl":"10.1016/j.tracli.2024.08.005","url":null,"abstract":"<p><strong>Introduction: </strong>Blood request form (BRF) stands as a pivotal document in ensuring safe and effective blood transfusions within healthcare settings. Incomplete or erroneous data on BRF can heighten risk of adverse reactions and compromise patient safety. Aim of study was to assess level of completion of BRFs by clinicians and to evaluate root cause analysis (RCA) of incompleteness of BRFs and factors leading to their rejection.</p><p><strong>Materials and methods: </strong>This prospective study was carried out from February 2024 to April 2024 on BRFs received in the blood centre. They were audited and RCA for factors leading to their incompleteness and rejection were analysed.</p><p><strong>Results: </strong>Total number of BRFs received in blood centre was 14,468. 13,358 (92.3%) BRFs were accepted and 1,110 (7.7%) BRFs were rejected. 12,804 (95.85%) of accepted BRFs were incomplete. Weight was the most common missing parameter (89% {n = 11403}) while name of the requesting clinician was least common (2.5% {n-318}). 3.52% n = 510) BRFs were rejected due to mismatch in name and patient registration number on BRF and samples. 0.14% n = 21) BRFs were rejected due to hemolysed samples. RCA for incompleteness of BRFs showed that main reason was manpower (61-83%) while environment was least common (17-67%). RCA for rejection of BRFs showed that environment was most common cause (13.3-80.15%) while manpower was least common (9-19.85%).</p><p><strong>Conclusion: </strong>Regular audits and personnel training, and quality assurance measures can help identify and address deficiencies in BRF completion to enhance patient safety and reduce incidence of transfusion-related errors and complications.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142147270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the potential of blood donors negative for high prevalence Rh antigens: A database initiative for thalassaemia care.","authors":"Akarshan Gupta, Davood Bava, Pandeep Kaur, Amit Kumar Chatterjee, Amit Kumar, Ankita Nigam, Anuneet Tripathi, Rakesh Kumar","doi":"10.1016/j.tracli.2024.08.004","DOIUrl":"10.1016/j.tracli.2024.08.004","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background and objectives: &lt;/strong&gt;With increasing life expectancy and prevalence of thalassaemia, it has led to a greater need for safe blood, yet the current supply from voluntary donors is insufficient to meet this demand. Thalassaemia recipients face a significant risk of alloimmunization because of repeated exposure to foreign red cell antigens. Study aims to determine high prevalent Rh antigen negative donors in western India donor population along with what percentage of these donors are willing to become dedicated voluntary donors for thalassaemia patients. Study also aims to examine factors influencing their willingness and challenges faced in mobilizing dedicated donors.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Method: &lt;/strong&gt;700 whole blood donors from western India, following screening for inclusion & exclusion criteria as per Drugs and Cosmetic Act (DCA) 2020 amendment guidelines & were sero-negative for transfusion transmitted infections were enrolled for the study. Red cell phenotyping was performed using Conventional Tube Technique (CTT) for \"D\", \"C\", \"E\", \"c\", \"e\" and \"K\" antigen using known antisera. Donors that were \"C\" AND/OR \"e\" antigen negative were contacted telephonically and were counseled and motivated for becoming voluntary blood donors. Statistical analysis assessed correlation between donation frequency, donor's occupation and education.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Result: &lt;/strong&gt;Among 700 donors, 96.6% (n = 676) were males and 3.4% (n = 24) were females. The most predominant blood group was B &gt; O &gt; A &gt; AB. Rh(D) antigen was present in 91.44% (n = 640) and absent in 8.6% (n = 60). Prevalence of other Rh antigens is as follows: \"e\" (99%) &gt; \"C\" (85.4%) &gt; \"c\" (59.1%) &gt; \"E\" (18.0%). Only 1.15% had \"K\" antigen positive. The commonest Rh phenotype R&lt;sub&gt;1&lt;/sub&gt;R&lt;sub&gt;1&lt;/sub&gt; (DCe/DCe) was expressed by 40.57% (n = 283), and the least common r″r (cE/ce), r″r″ (cE/cE) and r'r' (Ce/Ce) was found in 0.14% (n = 1), respectively. 'C' negative, 'e' negative, 'C' and 'e' antigen negative donors constituted 14.8% (n = 104) with 93.2% (n = 97) C-antigen negative, 1.92% (n = 2) e-antigen negative and 4.8% (n = 5) both \"C\" and \"e\" antigen negative donors. The commonest phenotypes among C-antigen and e-antigen negative donors were rr (50%) and R&lt;sub&gt;z&lt;/sub&gt;R&lt;sub&gt;2&lt;/sub&gt; (1.94%) respectively. Likewise, the most common phenotype amongst both C- and e-antigens negative donors was R&lt;sub&gt;2&lt;/sub&gt;R&lt;sub&gt;2&lt;/sub&gt; (3.84%). 61.5% of the donors agreed to enroll for voluntary blood donation following telephonic invitation, while 6.8% of them refused permanently. Approximately, 3.9% of the blood donors were willing to donate blood only when needed and 27.8% of them could not be contacted.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Creating a database of voluntary donors with known phenotype, especially who lack very common antigens like \"C\" and \"e\" and are willing to become dedicated, regular voluntary donors for thalassemic patients can ensure timely administration of safe blood. One ","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142001672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of oxidative and biochemical parameters of red cell concentrates (RCCs) prepared from G6PD deficient donors and healthy donors during RCC storage.","authors":"Samira Moshkelgosha, Mohammad Reza Deyhim, Ramazan Ali Khavari-Nejad, Mahdieh Meschi","doi":"10.1016/j.tracli.2024.08.003","DOIUrl":"10.1016/j.tracli.2024.08.003","url":null,"abstract":"<p><strong>Introduction: </strong>Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common inherited enzyme disorder in red blood cell (RBC). Due to the importance of G6PD enzyme as an antioxidant in RBC, we tried to investigate the oxidative damage in red cell concentrates (RCCs) prepared from donors with G6PD enzyme deficiency in comparison with healthy donors.</p><p><strong>Material method: </strong>This cross-sectional study was conducted on 20 male donors. Ten of the donors had G6PD deficiency (as a case) and the others had normal enzyme activity (as a control). Biochemical and oxidative damage parameters were examined in RCCs prepared from two groups on days 0, 7, 14, 21, 28 and 35 of RCCs storage; data comparison was analyzed by SPSS statistical software.</p><p><strong>Results: </strong>According to the result, lactate concentration increased significantly from the 7th day to the 35th day of RCC storage in G6PD-deficient donors compared to the control (P < 0.05). In addition, malondialdehyde (MDA) concentration in G6PD-deficient RCC showed a significant increase compared to the control in all days of storage (P < 0.05). Among the hematological parameters, mean corpuscular volume (MCV) and mean cell hemoglobin (MCH) increased significantly in all days of RCC storage in G6PD-deficient donors compared to the control (P < 0.05).</p><p><strong>Conclusion: </strong>Our study showed that oxidative changes in G6PD-deficient donors were significantly increased compared to the healthy donors, which probably leads to RCC storage lesion and an increase in blood transfusion complications. Due to the high prevalence of G6PD enzyme deficiency in pandemic areas, it seems that enzyme screening should be included in donor screening programs.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemovigilance and artificial intelligence: A way forward.","authors":"Radheshyam Meher","doi":"10.1016/j.tracli.2024.08.002","DOIUrl":"10.1016/j.tracli.2024.08.002","url":null,"abstract":"","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telemedicine in transfusion medicine: Bridging gaps in access, compliance, and expertise in the remote high-altitude hilly regions of Uttarakhand, India.","authors":"Manish Raturi, Yashaswi Dhiman, Dushyant Singh Gaur, Adityaveer Sahrawat","doi":"10.1016/j.tracli.2024.08.001","DOIUrl":"10.1016/j.tracli.2024.08.001","url":null,"abstract":"","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of acute radiation syndrome.","authors":"Pierre Arnautou, Guillaume Garnier, Jean Maillot, Johanna Konopacki, Michel Brachet, Annabelle Bonnin, Jean-Christophe Amabile, Jean-Valère Malfuson","doi":"10.1016/j.tracli.2024.07.002","DOIUrl":"10.1016/j.tracli.2024.07.002","url":null,"abstract":"<p><p>Acute radiation syndrome encompasses a spectrum of pathological manifestations resulting from exposure to high doses of ionizing radiation. This syndrome typically progresses through three stages with a prodromal phase, a latency phase and a critical phase. Each of them varies in intensity and duration depending on the absorbed dose of radiation. Predominantly affecting the bone marrow, skin, and gastrointestinal tract, its clinical implications are profound and multiorgan failure must be considered. Radiation doses below 2 Gray generally result in insignificant clinical consequences, while exposures surpassing 12 Gray exceeds current therapeutic capacities. Survival outcomes for patients within this therapeutic range depend on their ability to withstand radiation-induced aplasia, compounded by an increased risk of bleeding and infection due to skin, gastrointestinal, and potentially combined radiation injuries. Assessing the degree of radiation exposure plays a pivotal role in tailoring patient management strategies and is based on a combination of clinical, biological, and physical parameters. Treatment approaches primarily include intensive hematologic support to manage symptomatic manifestations and etiologic treatment is now based on the administration of growth factors. The role of hematopoietic stem cell transplant (HSCT) will be carefully considered on an individual basis, especially for patients who do not respond following 3 weeks of cytokine therapy. This review highlights the pathophysiological mechanisms, assessment modalities, and therapeutic interventions crucial for managing acute radiation syndrome aiming to optimize patient outcomes and guide clinical practice.</p>","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141725435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the Microscope: A memoir of Professor Sudha S. Bhat, an iconic Indian pathologist, and a transfusion medicine specialist.","authors":"Manish Raturi, Aditya Shivhare, Shamee Shastry","doi":"10.1016/j.tracli.2024.07.001","DOIUrl":"https://doi.org/10.1016/j.tracli.2024.07.001","url":null,"abstract":"","PeriodicalId":94255,"journal":{"name":"Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}