Stem cells and development最新文献_第3页

The Construction of Stem Cell-Induced Hepatocyte Model and Its Application in Evaluation of Developmental Hepatotoxicity of Environmental Pollutants. 干细胞诱导肝细胞模型的构建及其在环境污染物发育期肝毒性评估中的应用

Stem cells and development Pub Date : 2024-11-01 Epub Date: 2024-08-23 DOI: 10.1089/scd.2024.0117

Nadire Nijiati, Dilixiati Wubuli, Xiaobing Li, Zidong Zhou, Mulati Julaiti, Pengfei Huang, Bowen Hu

{"title":"The Construction of Stem Cell-Induced Hepatocyte Model and Its Application in Evaluation of Developmental Hepatotoxicity of Environmental Pollutants.","authors":"Nadire Nijiati, Dilixiati Wubuli, Xiaobing Li, Zidong Zhou, Mulati Julaiti, Pengfei Huang, Bowen Hu","doi":"10.1089/scd.2024.0117","DOIUrl":"10.1089/scd.2024.0117","url":null,"abstract":"Stem cells, with their ability to self-renew and differentiate into various cell types, are a unique and valuable resource for medical research and toxicological studies. The liver is the most crucial metabolic organ in the human body and serves as the primary site for the accumulation of environmental pollutants. Enrichment with environmental pollutants can disrupt the early developmental processes of the liver and have a significant impact on liver function. The liver comprises a complex array of cell types, and different environmental pollutants have varying effects on these cells. Currently, there is a lack of well-established research models that can effectively demonstrate the mechanisms by which environmental pollutants affect human liver development. The emergence of liver cells and organoids derived from stem cells offers a promising tool for investigating the impact of environmental pollutants on human health. Therefore, this study systematically reviewed the developmental processes of different types of liver cells and provided an overview of studies on the developmental toxicity of various environmental pollutants using stem cell models.","PeriodicalId":94214,"journal":{"name":"Stem cells and development","volume":" ","pages":"575-585"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141899271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

YAP Alleviates Pulmonary Fibrosis Through Promoting Alveolar Regeneration via Modulating the Stemness of Alveolar Type 2 Cells. YAP通过调节肺泡2型细胞的干性促进肺泡再生，从而缓解肺纤维化。

Stem cells and development Pub Date : 2024-11-01 Epub Date: 2024-08-23 DOI: 10.1089/scd.2024.0101

Jingyu Wang, Fengqing Zhu, Renru Luo, Yingyin Cui, Ziyu Zhang, Mengling Xu, Yuanyuan Zhao, Yonghui He, Wenqing Yang, Nianle Li, Zhu Zhu, Yingshan Chen, Tao Wang, Xuan Jiang, Chuwen Lin

{"title":"YAP Alleviates Pulmonary Fibrosis Through Promoting Alveolar Regeneration via Modulating the Stemness of Alveolar Type 2 Cells.","authors":"Jingyu Wang, Fengqing Zhu, Renru Luo, Yingyin Cui, Ziyu Zhang, Mengling Xu, Yuanyuan Zhao, Yonghui He, Wenqing Yang, Nianle Li, Zhu Zhu, Yingshan Chen, Tao Wang, Xuan Jiang, Chuwen Lin","doi":"10.1089/scd.2024.0101","DOIUrl":"10.1089/scd.2024.0101","url":null,"abstract":"Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with no cure except transplantation. Abnormal alveolar epithelial regeneration is a key driver of IPF development. The function of Yes1 Associated Transcriptional Regulator (YAP) in alveolar regeneration and IPF pathogenesis remains elusive. Here, we first revealed the activation of YAP in alveolar epithelium 2 cells (AEC2s) from human IPF lungs and fibrotic mouse lungs. Notably, conditional deletion of YAP in mouse AEC2s exacerbated bleomycin-induced pulmonary fibrosis. Intriguingly, we showed in both conditional knockout mice and alveolar organoids that YAP deficiency impaired AEC2 proliferation and differentiation into alveolar epithelium 1 cells (AEC1s). Mechanistically, YAP regulated expression levels of genes associated with cell cycle progression and AEC1 differentiation. Furthermore, overexpression of YAP in vitro promoted cell proliferation. These results indicate the critical role of YAP in alveolar regeneration and IPF pathogenesis. Our findings provide new insights into the regulation of alveolar regeneration and IPF pathogenesis, paving the road for developing novel treatment strategies.","PeriodicalId":94214,"journal":{"name":"Stem cells and development","volume":" ","pages":"586-594"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics Dysfunction in Replicative Senescence of Periodontal Ligament Stem Cells Regulated by AMPK Signaling Pathway. AMPK 信号通路调控牙周韧带干细胞复制衰老过程中的代谢组学功能障碍

Stem cells and development Pub Date : 2024-11-01 Epub Date: 2024-09-30 DOI: 10.1089/scd.2024.0112

Meilin Hu, Ruiqi Liu, Xiaoyu Chen, Shen Yan, Jian Gao, Yao Zhang, Di Wu, Lu Sun, Zhi Jia, Guangyunhao Sun, Dayong Liu

{"title":"Metabolomics Dysfunction in Replicative Senescence of Periodontal Ligament Stem Cells Regulated by AMPK Signaling Pathway.","authors":"Meilin Hu, Ruiqi Liu, Xiaoyu Chen, Shen Yan, Jian Gao, Yao Zhang, Di Wu, Lu Sun, Zhi Jia, Guangyunhao Sun, Dayong Liu","doi":"10.1089/scd.2024.0112","DOIUrl":"10.1089/scd.2024.0112","url":null,"abstract":"Periodontal ligament mesenchymal stem cells (PDLSCs) are a promising cell resource for stem cell-based regenerative medicine in dentistry, but they inevitably acquire a senescent phenotype after prolonged in vitro expansion. The key regulators of PDLSCs during replicative senescence remain unclear. Here, we sought to elucidate the role of metabolomic changes in determining the cellular senescence of PDLSCs. PDLSCs were cultured to passages 4, 10, and 20. The senescent phenotypes of PDLSCs were detected, and metabolomics analysis was performed. We found that PDLSCs manifested senescence phenotype during passaging. Metabolomics analysis showed that the metabolism of replicative senescence in PDLSCs varied significantly. The AMP-activated protein kinase (AMPK) signaling pathway was closely related to adenosine monophosphate (AMP) levels. The AMP:ATP ratio increased in senescent PDLSCs; however, the levels of p-AMPK, FOXO1 and FOXO3a decreased with senescence. We treated PDLSCs with an activator of the AMPK pathway (AICAR) and observed that the phosphorylated AMPK level at P20 PDLSCs was partially restored. These data delineate that the metabolic process of PDLSCs is active in the early stage of senescence and attenuated in the later stages of senescence; however, the sensitivity of AMPK phosphorylation sites is impaired, causing senescent PDLSCs to fail to respond to changes in energy metabolism.","PeriodicalId":94214,"journal":{"name":"Stem cells and development","volume":" ","pages":"607-615"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Different Developmental Stages on Bone Regeneration of Periodontal Ligament Stem Cells and Periodontal Ligament Cell Sheets In Vitro and Vivo. 不同发育阶段对牙周韧带干细胞和牙周韧带细胞片体内外骨再生的影响

Stem cells and development Pub Date : 2024-11-01 Epub Date: 2024-08-21 DOI: 10.1089/scd.2024.0087

Xin Shao, Fan Wu, Yang Song, Rongrong Kong, Shuang Wang, Liying Wang

{"title":"The Effects of Different Developmental Stages on Bone Regeneration of Periodontal Ligament Stem Cells and Periodontal Ligament Cell Sheets In Vitro and Vivo.","authors":"Xin Shao, Fan Wu, Yang Song, Rongrong Kong, Shuang Wang, Liying Wang","doi":"10.1089/scd.2024.0087","DOIUrl":"10.1089/scd.2024.0087","url":null,"abstract":"Periodontal ligament stem cells (PDLSCs) have broad applications in tissue engineering and regeneration. However, the function of PDLSCs changes in different microenvironments. This study aimed to explore the effects of different developmental stages on the biological characteristics of PDLSCs. Here, PDLSCs were successfully cultured from the periodontal tissues of various groups, including a group with immature roots, a young group with mature roots, and an aging group with mature roots. By comparing the results of the three experimental groups, we found that the donors with immature roots exhibited the best proliferative ability and osteogenic differentiation, whereas the aging group demonstrated the worst proliferation. The trend for adipogenic differentiation was the opposite to that for osteogenic differentiation. The cell sheet and in vivo experiments revealed that in the immature root group, the cells produced more extracellular matrix and new bone and better absorbed the implant materials. These results indicate that PDLSCs perform various functions at different stages of development. In clinical applications of PDLSCs for periodontal regeneration, donors with incompletely developed roots have stronger biological characteristics.","PeriodicalId":94214,"journal":{"name":"Stem cells and development","volume":" ","pages":"595-606"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dtx2 Deficiency Induces Ependymo-Radial Glial Cell Proliferation and Improves Spinal Cord Motor Function Recovery. Dtx2 缺乏会诱导上皮神经胶质细胞增殖，并改善脊髓运动功能的恢复。

Stem cells and development Pub Date : 2024-10-01 Epub Date: 2024-08-09 DOI: 10.1089/scd.2023.0247

Hao-Yuan Chen, Yin-Cheng Huang, Tu-Hsueh Yeh, Chia-Wei Chang, Yang-Jin Shen, Yi-Chieh Chen, Mu-Qun Sun, Yi-Chuan Cheng

{"title":"Dtx2 Deficiency Induces Ependymo-Radial Glial Cell Proliferation and Improves Spinal Cord Motor Function Recovery.","authors":"Hao-Yuan Chen, Yin-Cheng Huang, Tu-Hsueh Yeh, Chia-Wei Chang, Yang-Jin Shen, Yi-Chieh Chen, Mu-Qun Sun, Yi-Chuan Cheng","doi":"10.1089/scd.2023.0247","DOIUrl":"10.1089/scd.2023.0247","url":null,"abstract":"Traumatic injury to the spinal cord can lead to significant, permanent disability. Mammalian spinal cords are not capable of regeneration; in contrast, adult zebrafish are capable of such regeneration, fully recovering motor function. Understanding the mechanisms underlying zebrafish neuroregeneration may provide useful information regarding endogenous regenerative potential and aid in the development of therapeutic strategies in humans. DELTEX proteins (DTXs) regulate a variety of cellular processes. However, their role in neural regeneration has not been described. We found that zebrafish dtx2, encoding Deltex E3 ubiquitin ligase 2, is expressed in ependymo-radial glial cells in the adult spinal cord. After spinal cord injury, the heterozygous dtx2 mutant fish motor function recovered quicker than that of the wild-type controls. The mutant fish displayed increased ependymo-radial glial cell proliferation and augmented motor neuron formation. Moreover, her gene expression, downstream of Notch signaling, increased in Dtx2 mutants. Notch signaling inactivation by dominant-negative Rbpj abolished the increased ependymo-radial glia proliferation caused by Dtx2 deficiency. These results indicate that ependymo-radial glial proliferation is induced by Dtx2 deficiency by activating Notch-Rbpj signaling to improve spinal cord regeneration and motor function recovery.","PeriodicalId":94214,"journal":{"name":"Stem cells and development","volume":" ","pages":"540-550"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Endothelial Cell Is Not Simply an Endothelial Cell. 内皮细胞不仅仅是内皮细胞。

Stem cells and development Pub Date : 2024-10-01 Epub Date: 2024-08-09 DOI: 10.1089/scd.2024.0088

Shiwani Limbu, Kara E McCloskey

引用次数: 0

Stanniocalcin 2 Promotes Neuronal Differentiation in Neural Stem/Progenitor Cells of the Mouse Subventricular Zone Through Activation of AKT Pathway. Stanniocalcin 2通过激活AKT通路促进小鼠脑室下区神经干/祖细胞的神经元分化

Stem cells and development Pub Date : 2024-10-01 Epub Date: 2024-08-09 DOI: 10.1089/scd.2024.0094

Zhenyu Guo, Hanyue Zhang, Xinbate Jingele, Jing Yan, Xinxiang Wang, Yingxi Liu, Tingqin Huang, Chongxiao Liu

{"title":"Stanniocalcin 2 Promotes Neuronal Differentiation in Neural Stem/Progenitor Cells of the Mouse Subventricular Zone Through Activation of AKT Pathway.","authors":"Zhenyu Guo, Hanyue Zhang, Xinbate Jingele, Jing Yan, Xinxiang Wang, Yingxi Liu, Tingqin Huang, Chongxiao Liu","doi":"10.1089/scd.2024.0094","DOIUrl":"10.1089/scd.2024.0094","url":null,"abstract":"Neural stem/progenitor cells (NSPCs) persist in the mammalian subventricular zone (SVZ) throughout life, responding to various pathophysiological stimuli and playing a crucial role in central nervous system repair. Although numerous studies have elucidated the role of stanniocalcin 2 (STC2) in regulating cell differentiation processes, its specific function in NSPCs differentiation remains poorly understood. Clarifying the role of STC2 in NSPCs is essential for devising novel strategies to enhance the intrinsic potential for brain regeneration postinjury. Our study revealed the expression of STC2 in NSPCs derived from the SVZ of the C57BL/6N mouse. In cultured SVZ-derived NSPCs, STC2 treatment significantly increased the number of Tuj1 and DCX-positive cells. Furthermore, STC2 injection into the lateral ventricle promoted the neuronal differentiation of NSPCs and migration to the olfactory bulb. Conversely, the STC2 knockdown produced the opposite effect. Further investigation showed that STC2 treatment enhanced AKT phosphorylation in cultured NSPCs, whereas STC2 inhibition hindered AKT activation. Notably, the neuronal differentiation induced by STC2 was blocked by the AKT inhibitor GSK690693, whereas the AKT activator SC79 reversed the impact of STC2 knockdown on neuronal differentiation. Our findings indicate that enhancing STC2 expression in SVZ-derived NSPCs facilitates neuronal differentiation, with AKT regulation potentially serving as a key intracellular target of STC2 signaling.","PeriodicalId":94214,"journal":{"name":"Stem cells and development","volume":" ","pages":"551-561"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141725429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling Choroideremia Disease with Isogenic Induced Pluripotent Stem Cells. 用异源诱导多能干细胞模拟脉络膜血症。

Stem cells and development Pub Date : 2024-10-01 Epub Date: 2024-08-16 DOI: 10.1089/scd.2024.0105

Ana Fragoso Fonseca, Rita Coelho, Mafalda Lopes- da-Silva, Luísa Lemos, Michael J Hall, Daniela Oliveira, Ana Sofia Falcão, Sandra Tenreiro, Miguel C Seabra, Pedro Antas

{"title":"Modeling Choroideremia Disease with Isogenic Induced Pluripotent Stem Cells.","authors":"Ana Fragoso Fonseca, Rita Coelho, Mafalda Lopes- da-Silva, Luísa Lemos, Michael J Hall, Daniela Oliveira, Ana Sofia Falcão, Sandra Tenreiro, Miguel C Seabra, Pedro Antas","doi":"10.1089/scd.2024.0105","DOIUrl":"10.1089/scd.2024.0105","url":null,"abstract":"Choroideremia (CHM) is a rare X-linked chorioretinal dystrophy causing progressive vision loss due to mutations in the CHM gene, leading to Rab escort protein 1 loss of function. CHM disease is characterized by a progressive degeneration of the choroid, the retinal pigment epithelium (RPE), and the retina. The RPE is a monolayer of polarized cells that supports photoreceptors, providing nutrients, growth factors, and ions, and removes retinal metabolism waste products, having a central role in CHM pathogenesis. Commonly used models such as ARPE-19 cells do not reproduce accurately the nature of RPE cells. Human induced pluripotent stem cells (hiPSCs) can be differentiated into RPE cells (hiPSC-RPE), which mimic key features of native RPE, being more suited to study retinal diseases. Therefore, we took advantage of hiPSCs to generate new human-based CHM models. Two isogenic hiPSC lines were generated through CRISPR/Cas9: a CHM knock-out line from a healthy donor and a corrected CHM patient line using a knock-in approach. The differentiated hiPSC-RPE lines exhibited critical morphological and physiological characteristics of native RPE, including the presence of the tight junction markers Claudin-19 and Zonula Occludens-1, phagocytosis of photoreceptor outer segments, pigmentation, a postmitotic state, and the characteristic polygonal shape. In addition, all the studied cells were able to form retinal organoids. This work resulted in the establishment of isogenic hiPSC lines, representing a new and important CHM cellular model. To our knowledge, this is the first time that isogenic cell lines have been developed to model CHM disease, providing a valuable tool for studying the mechanisms at the onset of RPE degeneration.","PeriodicalId":94214,"journal":{"name":"Stem cells and development","volume":" ","pages":"528-539"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141794457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cleft Palate Induced by Augmented Fibroblast Growth Factor-9 Signaling in Cranial Neural Crest Cells in Mice. 小鼠颅神经嵴细胞中成纤维细胞生长因子-9信号增强诱发腭裂。

Stem cells and development Pub Date : 2024-10-01 Epub Date: 2024-08-22 DOI: 10.1089/scd.2024.0077

Chensheng Lin, Shiyu Liu, Ningsheng Ruan, Jiang Chen, YiPing Chen, Yanding Zhang, Jian Zhang

{"title":"Cleft Palate Induced by Augmented Fibroblast Growth Factor-9 Signaling in Cranial Neural Crest Cells in Mice.","authors":"Chensheng Lin, Shiyu Liu, Ningsheng Ruan, Jiang Chen, YiPing Chen, Yanding Zhang, Jian Zhang","doi":"10.1089/scd.2024.0077","DOIUrl":"10.1089/scd.2024.0077","url":null,"abstract":"Although enhanced fibroblast growth factor (FGF) signaling has been demonstrated to be crucial in many cases of syndromic cleft palate caused by tongue malposition in humans, animal models that recapitulate this phenotype are limited, and the precise mechanisms remain elusive. Mutations in FGF9 with the effect of either loss- or gain-of-function effects have been identified to be associated with cleft palate in humans. Here, we generated a mouse model with a transgenic Fgf9 allele specifically activated in cranial neural crest cells, aiming to elucidate the gain-of-function effects of Fgf9 in palatogenesis. We observed cleft palate with 100% penetrance in mutant mice. Further analysis demonstrated that no inherent defects in the morphogenic competence of palatal shelves could be found, but a passively lifted tongue prevented the elevation of palatal shelves, leading to the cleft palate. This tongue malposition was induced by posterior spatial confinement that was exerted by temporomandibular joint (TMJ) dysplasia characterized by a reduction in Sox9+ progenitors within the condyle and a structural decrease in the posterior dimension of the lower jaw. Our findings highlight the critical role of excessive FGF signaling in disrupting spatial coordination during palate development and suggest a potential association between palatal shelf elevation and early TMJ development.","PeriodicalId":94214,"journal":{"name":"Stem cells and development","volume":" ","pages":"562-573"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiation, Metabolism, and Cardioprotective Secretory Functions of Human Cardiac Stromal Cells from Ischemic and Endocarditis Patients. 来自缺血和心内膜炎患者的人类心脏基质细胞的分化、代谢和心脏保护分泌功能。

Stem cells and development Pub Date : 2024-09-01 Epub Date: 2024-07-16 DOI: 10.1089/scd.2024.0103

Helen Nguyen, Chuan-Chih Hsu, Annette Meeson, Rachel Oldershaw, Gavin Richardson, Andreas Czosseck, David J Lundy

{"title":"Differentiation, Metabolism, and Cardioprotective Secretory Functions of Human Cardiac Stromal Cells from Ischemic and Endocarditis Patients.","authors":"Helen Nguyen, Chuan-Chih Hsu, Annette Meeson, Rachel Oldershaw, Gavin Richardson, Andreas Czosseck, David J Lundy","doi":"10.1089/scd.2024.0103","DOIUrl":"10.1089/scd.2024.0103","url":null,"abstract":"This study investigates the characteristics of cardiac mesenchymal stem cell-like cells (CMSCLCs) isolated from the right atrial appendage of human donors with ischemia and a young patient with endocarditis (NE-CMSCLCs). Typical CMSCLCs from ischemic heart patients were derived from coronary artery bypass grafting procedures and compared against bone marrow mesenchymal stromal cells (BM-MSCs). NE-CMSCLCs had a normal immunophenotype, but exhibited enhanced osteogenic differentiation potential, rapid proliferation, reduced senescence, reduced glycolysis, and lower reactive oxygen species generation after oxidative stress compared with typical ischemic CMSCLCs. These differences suggest a unique functional status of NE-CMSCLCs, influenced by the donor health condition. Despite large variances in their paracrine secretome, NE-CMSCLCs retained therapeutic potential, as indicated by their ability to protect hypoxia/reoxygenation-injured human cardiomyocytes, albeit less effectively than typical CMSCLCs. This research describes a unique cell phenotype and underscores the importance of donor health status in the therapeutic efficacy of autologous cardiac cell therapy.","PeriodicalId":94214,"journal":{"name":"Stem cells and development","volume":" ","pages":"484-495"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141474186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0