mLife Pub Date : 2023-06-30 eCollection Date: 2023-06-01 DOI: 10.1002/mlf2.12075

Yuan Yao, Hongwei Sun, Wurihan, Gegeheng, Gezi, Kirsten Skarstad, Lifei Fan, Morigen

引用次数: 0

A bacterial symbiont in the gill of the marine scallop Argopecten irradians irradians metabolizes dimethylsulfoniopropionate. 海洋扇贝 Argopecten irradians irradians 鳃中的一种细菌共生体代谢二甲基硫代丙酸盐。

mLife Pub Date : 2023-06-26 eCollection Date: 2023-06-01 DOI: 10.1002/mlf2.12072

Yi Shu, Yongming Wang, Zhongcheng Wei, Ning Gao, Shuyan Wang, Chun-Yang Li, Qiang Xing, Xiaoli Hu, Xiao-Hua Zhang, Yu-Zhong Zhang, Weipeng Zhang, Zhenmin Bao, Wei Ding

{"title":"A bacterial symbiont in the gill of the marine scallop Argopecten irradians irradians metabolizes dimethylsulfoniopropionate.","authors":"Yi Shu, Yongming Wang, Zhongcheng Wei, Ning Gao, Shuyan Wang, Chun-Yang Li, Qiang Xing, Xiaoli Hu, Xiao-Hua Zhang, Yu-Zhong Zhang, Weipeng Zhang, Zhenmin Bao, Wei Ding","doi":"10.1002/mlf2.12072","DOIUrl":"10.1002/mlf2.12072","url":null,"abstract":"Microbial lysis of dimethylsulfoniopropionate (DMSP) is a key step in marine organic sulfur cycling and has been recently demonstrated to play an important role in mediating interactions between bacteria, algae, and zooplankton. To date, microbes that have been found to lyse DMSP are largely confined to free-living and surface-attached bacteria. In this study, we report for the first time that a symbiont (termed \"Rhodobiaceae bacterium HWgs001\") in the gill of the marine scallop Argopecten irradians irradians can lyse and metabolize DMSP. Analysis of 16S rRNA gene sequences suggested that HWgs001 accounted for up to 93% of the gill microbiota. Microscopic observations suggested that HWgs001 lived within the gill tissue. Unlike symbionts of other bivalves, HWgs001 belongs to Alphaproteobacteria rather than Gammaproteobacteria, and no genes for carbon fixation were identified in its small genome. Moreover, HWgs001 was found to possess a dddP gene, responsible for the lysis of DMSP to acrylate. The enzymatic activity of dddP was confirmed using the heterologous expression, and in situ transcription of the gene in scallop gill tissues was demonstrated using reverse-transcription PCR. Together, these results revealed a taxonomically and functionally unique symbiont, which represents the first-documented DMSP-metabolizing symbiont likely to play significant roles in coastal marine ecosystems.","PeriodicalId":94145,"journal":{"name":"mLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10989825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85816004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The secretory Candida effector Sce1 licenses fungal virulence by masking the immunogenic β-1,3-glucan and promoting apoptosis of the host cells. 分泌型念珠菌效应物 Sce1 通过掩盖免疫原性 β-1,3-葡聚糖和促进宿主细胞凋亡来增强真菌的毒力。

mLife Pub Date : 2023-06-26 eCollection Date: 2023-06-01 DOI: 10.1002/mlf2.12066

Hongyu Wu, Li Wang, Wenjuan Wang, Zhugui Shao, Xin-Ming Jia, Hui Xiao, Jiangye Chen

{"title":"The secretory Candida effector Sce1 licenses fungal virulence by masking the immunogenic β-1,3-glucan and promoting apoptosis of the host cells.","authors":"Hongyu Wu, Li Wang, Wenjuan Wang, Zhugui Shao, Xin-Ming Jia, Hui Xiao, Jiangye Chen","doi":"10.1002/mlf2.12066","DOIUrl":"10.1002/mlf2.12066","url":null,"abstract":"Candida albicans deploys a variety of mechanisms such as morphological switch and elicitor release to promote virulence. However, the intricate interactions between the fungus and the host remain poorly understood, and a comprehensive inventory of fungal virulence factors has yet to be established. In this study, we identified a C. albicans secretory effector protein Sce1, whose induction and secretion are associated with vagina-simulative conditions and chlamydospore formation. Sequence alignment showed that Sce1 belongs to a Pir family in C. albicans, which is conserved across several fungi and primarily characterized as a β-glucan binding protein in the Saccharomyces cerevisiae. Mechanically, Sce1 is primarily localized to the cell wall in a cleaved form as an alkali-labile β-1,3-glucan binding protein and plays a role in masking β-glucan in acidic environments and chlamydospores, a feature that might underline C. albicans' ability to evade host immunity. Further, a cleaved short form of Sce1 protein could be released into extracellular compartments and presented in bone marrow-derived macrophages infected with chlamydospores. This cleaved short form of Sce1 also demonstrated a unique ability to trigger the caspases-8/9-dependent apoptosis in various host cells. Correspondingly, genetic deletion of SCE1 led to dampened vaginal colonization of C. albicans and diminished fungal virulence during systemic infection. The discovery of Sce1 as a versatile virulence effector that executes at various compartments sheds light on the fungus-host interactions and C. albicans pathogenesis.","PeriodicalId":94145,"journal":{"name":"mLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10989805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85746122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial scaling of soil microbial co-occurrence networks in a fragmented landscape. 破碎景观中土壤微生物共生网络的空间尺度。

mLife Pub Date : 2023-06-26 eCollection Date: 2023-06-01 DOI: 10.1002/mlf2.12073

Pandeng Wang, Shao-Peng Li, Xian Yang, Xingfeng Si, Wen-Jun Li, Wensheng Shu, Lin Jiang

引用次数: 0

Synthetic biology to revive microbial natural product discovery. 合成生物学振兴微生物天然产品的发现。

mLife Pub Date : 2023-06-22 eCollection Date: 2023-06-01 DOI: 10.1002/mlf2.12071

Xiaoyu Tang

引用次数: 0

Single-cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste-modified soils. 单细胞拉曼和功能基因分析揭示了农业废弃物改良土壤中微生物对 P 的增溶作用。

mLife Pub Date : 2023-06-14 eCollection Date: 2023-06-01 DOI: 10.1002/mlf2.12053

Hongzhe Li, Jiazhi Ding, Longji Zhu, Fei Xu, Wenjing Li, Yanpo Yao, Li Cui

{"title":"Single-cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste-modified soils.","authors":"Hongzhe Li, Jiazhi Ding, Longji Zhu, Fei Xu, Wenjing Li, Yanpo Yao, Li Cui","doi":"10.1002/mlf2.12053","DOIUrl":"10.1002/mlf2.12053","url":null,"abstract":"Application of agricultural waste such as rapeseed meal (RM) is regarded as a sustainable way to improve soil phosphorus (P) availability by direct nutrient supply and stimulation of native phosphate-solubilizing microorganisms (PSMs) in soils. However, exploration of the in situ microbial P solubilizing function in soils remains a challenge. Here, by applying both phenotype-based single-cell Raman with D2O labeling (Raman-D2O) and genotype-based high-throughput chips targeting carbon, nitrogen and P (CNP) functional genes, the effect of RM application on microbial P solubilization in three typical farmland soils was investigated. The abundances of PSMs increased in two alkaline soils after RM application identified by single-cell Raman D2O. RM application reduced the diversity of bacterial communities and increased the abundance of a few bacteria with reported P solubilization function. Genotypic analysis indicated that RM addition generally increased the relative abundance of CNP functional genes. A correlation analysis of the abundance of active PSMs with the abundance of soil microbes or functional genes was carried out to decipher the linkage between the phenotype and genotype of PSMs. Myxococcota and C degradation genes were found to potentially contribute to the enhanced microbial P release following RM application. This work provides important new insights into the in situ function of soil PSMs. It will lead to better harnessing of agricultural waste to mobilize soil legacy P and mitigate the P crisis.","PeriodicalId":94145,"journal":{"name":"mLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10989763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85480673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human skin bacterial microbiota homeostasis: A delicate balance between health and disease. 人体皮肤细菌微生物群平衡：健康与疾病之间的微妙平衡

mLife Pub Date : 2023-06-04 eCollection Date: 2023-06-01 DOI: 10.1002/mlf2.12064

Yibin Zhu, Xi Yu, Gong Cheng

{"title":"Human skin bacterial microbiota homeostasis: A delicate balance between health and disease.","authors":"Yibin Zhu, Xi Yu, Gong Cheng","doi":"10.1002/mlf2.12064","DOIUrl":"10.1002/mlf2.12064","url":null,"abstract":"As the largest organ of the body, the skin acts as a barrier to prevent diseases and harbors a variety of beneficial bacteria. Furthermore, the skin bacterial microbiota plays a vital role in health and disease. Disruption of the barrier or an imbalance between symbionts and pathogens can lead to skin disorders or even systemic diseases. In this review, we first provide an overview of research on skin bacterial microbiota and human health, including the composition of skin bacteria in a healthy state, as well as skin bacterial microbiota educating the immune system and preventing the invasion of pathogens. We then discuss the diseases that result from skin microbial dysbiosis, including atopic dermatitis, common acne, chronic wounds, psoriasis, viral transmission, cutaneous lupus, cutaneous lymphoma, and hidradenitis suppurativa. Finally, we highlight the progress that utilizes skin microorganisms for disease therapeutics, such as bacteriotherapy and skin microbiome transplantation. A deeper knowledge of the interaction between human health and disease and the homeostasis of the skin bacterial microbiota will lead to new insights and strategies for exploiting skin bacteria as a novel therapeutic target.","PeriodicalId":94145,"journal":{"name":"mLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10989898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89653734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A host E3 ubiquitin ligase regulates Salmonella virulence by targeting an SPI‐2 effector involved in SIF biogenesis 宿主E3泛素连接酶通过靶向参与SIF生物发生的SPI‐2效应物来调节沙门氏菌的毒力

mLife Pub Date : 2023-06-01 DOI: 10.1002/mlf2.12063

Kun Meng, Jin Yang, Juan Xue, Jun Lv, Ping Zhu, Liuliu Shi, Shan Li

{"title":"A host E3 ubiquitin ligase regulates Salmonella virulence by targeting an SPI‐2 effector involved in SIF biogenesis","authors":"Kun Meng, Jin Yang, Juan Xue, Jun Lv, Ping Zhu, Liuliu Shi, Shan Li","doi":"10.1002/mlf2.12063","DOIUrl":"https://doi.org/10.1002/mlf2.12063","url":null,"abstract":"Abstract Salmonella Typhimurium creates an intracellular niche for its replication by utilizing a large cohort of effectors, including several that function to interfere with host ubiquitin signaling. Although the mechanism of action of many such effectors has been elucidated, how the interplay between the host ubiquitin network and bacterial virulence factors dictates the outcome of infection largely remains undefined. In this study, we found that the SPI‐2 effector SseK3 inhibits SNARE pairing to promote the formation of a Salmonella ‐induced filament by Arg‐GlcNAcylation of SNARE proteins, including SNAP25, VAMP8, and Syntaxin. Further study reveals that host cells counteract the activity of SseK3 by inducing the expression of the E3 ubiquitin ligase TRIM32, which catalyzes K48‐linked ubiquitination on SseK3 and targets its membrane‐associated portion for degradation. Hence, TRIM32 antagonizes SNAP25 Arg‐GlcNAcylation induced by SseK3 to restrict Salmonella ‐induced filament biogenesis and Salmonella replication. Our study reveals a mechanism by which host cells inhibit bacterial replication by eliminating specific virulence factors.","PeriodicalId":94145,"journal":{"name":"mLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new technique to ATTACK the silent pandemic of antimicrobial resistance. 攻克抗菌药耐药性这一无声流行病的新技术。

mLife Pub Date : 2023-05-17 eCollection Date: 2023-06-01 DOI: 10.1002/mlf2.12065

Yong-Guan Zhu

引用次数: 0

Beyond biogeographic patterns: Processes shaping the microbial landscape in soils and sediments along the Yangtze River. 超越生物地理模式：塑造长江沿岸土壤和沉积物微生物景观的过程。

mLife Pub Date : 2023-03-26 eCollection Date: 2023-03-01 DOI: 10.1002/mlf2.12062

Wenjie Wan, Geoffrey M Gadd, Ji-Dong Gu, Wenzhi Liu, Peng Chen, Quanfa Zhang, Yuyi Yang

{"title":"Beyond biogeographic patterns: Processes shaping the microbial landscape in soils and sediments along the Yangtze River.","authors":"Wenjie Wan, Geoffrey M Gadd, Ji-Dong Gu, Wenzhi Liu, Peng Chen, Quanfa Zhang, Yuyi Yang","doi":"10.1002/mlf2.12062","DOIUrl":"10.1002/mlf2.12062","url":null,"abstract":"Deciphering biogeographic patterns of microorganisms is important for evaluating the maintenance of microbial diversity with respect to the ecosystem functions they drives. However, ecological processes shaping distribution patterns of microorganisms across large spatial-scale watersheds remain largely unknown. Using Illumina sequencing and multiple statistical methods, we characterized distribution patterns and maintenance diversity of microorganisms (i.e., archaea, bacteria, and fungi) in soils and sediments along the Yangtze River. Distinct microbial distribution patterns were found between soils and sediments, and microbial community similarity significantly decreased with increasing geographical distance. Physicochemical properties showed a larger effect on microbial community composition than geospatial and climatic factors. Archaea and fungi displayed stronger species replacements and weaker environmental constraints in soils than that in sediments, but opposite for bacteria. Archaea, bacteria, and fungi in soils showed broader environmental breadths and stronger phylogenetic signals compared to those in sediments, suggesting stronger environmental adaptation. Stochasticity dominated community assemblies of archaea and fungi in soils and sediments, whereas determinism dominated bacterial community assembly. Our results have therefore highlighted distinct microbial distribution patterns and diversity maintenance mechanisms between soils and sediments, and emphasized important roles of species replacement, environmental adaptability, and ecological assembly processes on microbial landscape. Our findings are helpful in predicting loss of microbial diversity in the Yangtze River Basin, and might assist the establishment of environmental policies for protecting fragile watersheds.","PeriodicalId":94145,"journal":{"name":"mLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10989888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80220552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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