Journal of dermatological science最新文献

{"title":"JSID flier with late breaking announce","authors":"","doi":"10.1016/S0923-1811(24)00186-5","DOIUrl":"10.1016/S0923-1811(24)00186-5","url":null,"abstract":"","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":"115 3","pages":"Page I"},"PeriodicalIF":4.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142244197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to volatile ferroptosis inhibitor, TEMPO, reduced cutaneous ischemia-reperfusion injury progression to pressure ulcer formation in a mouse model","authors":"Mai Ishikawa ,&nbsp;Akihiko Uchiyama ,&nbsp;Keiji Kosaka ,&nbsp;Mayu Nishio ,&nbsp;Sachiko Ogino ,&nbsp;Yoko Yokoyama ,&nbsp;Ryoko Torii ,&nbsp;Ryoko Akai ,&nbsp;Takao Iwawaki ,&nbsp;Seiji Torii ,&nbsp;Sei-ichiro Motegi","doi":"10.1016/j.jdermsci.2024.07.005","DOIUrl":"10.1016/j.jdermsci.2024.07.005","url":null,"abstract":"<div><h3>Background</h3><p>Ischemia– reperfusion (I/R) injury-induced oxidative stress is a key factor in the pathogenesis of pressure ulcer formation. Ferroptosis is an iron-dependent programmed cell death that connects oxidative stress and inflammation in various diseases. Recent studies revealed the protective effect of inhibition of ferroptosis in I/R injury. However, the role of ferroptosis in cutaneous I/R injury remains elusive.</p></div><div><h3>Objective</h3><p>To assess the role of ferroptosis in the progression of cutaneous I/R injury.</p></div><div><h3>Methods</h3><p>Cutaneous I/R injury experiments and histopathological studies were performed in wild-type mice with or without exposure to volatile ferroptosis inhibitor, TEMPO (2,2,6,6-Tetramethylpiperidine-1-oxyl). The suppressive effects of TEMPO on ferroptosis inducing cell death and oxidative stress were examined <em>in vitro</em>.</p></div><div><h3>Results</h3><p>Inhibition of ferroptosis with TEMPO significantly reduced ulcer formation after cutaneous I/R injury. Fluctuated ferroptosis markers, such as GPX4, ACSL4, and 4-HNE expression in the I/R skin site, were reversed by TEMPO treatment. Inhibition of ferroptosis reduced apoptosis, CD3⁺ infiltrating lymphocytes, and improved vascularity in the I/R skin site. Inhibition of ferroptosis also suppressed the enhancement of Nrf2 activation. <em>In vitro</em>, ferroptosis and the activation of ferroptosis-related gene expression by RSL3 stimulation were markedly ameliorated by TEMPO treatment in mouse fibroblasts. Inhibiting ferroptosis also suppressed the elevation of the mRNA levels of NOX2 and HO-1 caused by ferroptosis.</p></div><div><h3>Conclusion</h3><p>Cutaneous I/R injury-induced ferroptosis likely promotes cell death, vascular loss, infiltration of inflammatory cells, and oxidative stress. The inhibition of ferroptosis with TEMPO might have potential clinical application as novel therapeutic agent for cutaneous I/R injury.</p></div>","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":"115 3","pages":"Pages 130-140"},"PeriodicalIF":4.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923181124001518/pdfft?md5=ada58dbe07a87acdcb729adcccd7d6b0&pid=1-s2.0-S0923181124001518-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of an adeno-associated viral vector for epidermal keratinocytes in vitro and in vivo","authors":"Qi Shen ,&nbsp;Shogo Suga ,&nbsp;Yuta Moriwaki ,&nbsp;Zening Du ,&nbsp;Emi Aizawa ,&nbsp;Mutsumi Okazaki ,&nbsp;Juan Carlos Izpisua Belmonte ,&nbsp;Yusuke Hirabayashi ,&nbsp;Keiichiro Suzuki ,&nbsp;Masakazu Kurita","doi":"10.1016/j.jdermsci.2024.07.006","DOIUrl":"10.1016/j.jdermsci.2024.07.006","url":null,"abstract":"<div><h3>Background</h3><p>Local gene therapies, including <em>in vivo</em> genome editing, are highly anticipated for the treatment of genetic diseases in skin, especially the epidermis. While the adeno-associated virus (AAV) is a potent vector for <em>in vivo</em> gene delivery, the lack of efficient gene delivery methods has limited its clinical applications.</p></div><div><h3>Objective</h3><p>To optimize the AAV gene delivery system with higher gene delivery efficiency and specificity for epidermis and keratinocytes (KCs), using AAV capsid and promoter engineering technologies.</p></div><div><h3>Methods</h3><p>AAV variants with mutations in residues reported to be critical to determine the tropism of AAV2 for KCs were generated by site-directed mutagenesis of AAVDJ. The infection efficiency and specificity for KCs of these variants were compared with those of previously reported AAVs considered to be suitable for gene delivery to KCs <em>in vitro</em> and <em>in vivo</em>. Additionally, we generated an epidermis-specific promoter using the most recent short-core promoter and compared its specificity with existing promoters.</p></div><div><h3>Results</h3><p>A novel AAVDJ variant capsid termed AAVDJK2 was superior to the existing AAVs in terms of gene transduction efficiency and specificity for epidermis and KCs <em>in vitro</em> and <em>in vivo</em>. A novel tissue-specific promoter, termed the K14 SCP3 promoter, was superior to the existing promoters in terms of gene transduction efficiency and specificity for KCs.</p></div><div><h3>Conclusion</h3><p>The combination of the AAVDJK2 capsid and K14 SCP3 promoter improves gene delivery to epidermis <em>in vivo</em> and KCs <em>in vitro</em>. The novel AAV system may benefit experimental research and development of new epidermis-targeted gene therapies.</p></div>","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":"115 3","pages":"Pages 101-110"},"PeriodicalIF":4.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141914943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of skin barrier function and augmentation of epidermal cell-cell interactions by galactomyces ferment filtrate","authors":"Satoshi Nakamizo ,&nbsp;Xianghong Yan ,&nbsp;Kenji Kabashima","doi":"10.1016/j.jdermsci.2024.03.011","DOIUrl":"10.1016/j.jdermsci.2024.03.011","url":null,"abstract":"","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":"115 2","pages":"Pages 94-97"},"PeriodicalIF":4.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923181124000574/pdfft?md5=8d0fd39403ab5f87104e964e890f7e1d&pid=1-s2.0-S0923181124000574-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141629557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of line-field confocal optical coherence tomography and in situ microenvironmental mapping to investigate the living microenvironment of reconstructed human skin and melanoma models","authors":"Elisabetta Michielon ,&nbsp;Alexandre C. Motta ,&nbsp;Jonas Ogien ,&nbsp;Paul Oranje ,&nbsp;Taco Waaijman ,&nbsp;Varsha Thakoersing ,&nbsp;Sanne Veldhorst ,&nbsp;Tanja D. de Gruijl ,&nbsp;Susan Gibbs","doi":"10.1016/j.jdermsci.2024.07.001","DOIUrl":"10.1016/j.jdermsci.2024.07.001","url":null,"abstract":"<div><h3>Background</h3><p>In tissue engineering, real-time monitoring of tumors and of the dynamics of the microenvironment within <em>in vitro</em> models has traditionally been hindered by the need to harvest the cultures to obtain material to analyze. Line-field confocal optical coherence tomography (LC-OCT) has proven to be useful in evaluating <em>in vivo</em> skin conditions, including melanoma, by capturing dynamic, three-dimensional (3D) information without the need for invasive procedures, such as biopsies. Additionally, the M-Duo Technology® developed by IMcoMET presents a unique opportunity for continuous <em>in situ</em> biomarker sampling, providing insights into local cellular behavior and interactions.</p></div><div><h3>Objective</h3><p>This study aimed to validate the non-destructive mapping capabilities of two advanced methodologies (LC-OCT by DAMAE Medical and M-Duo Technology® by IMcoMET) to investigate the living microenvironment of <em>in vitro</em> reconstructed human skin (RhS) and melanoma-RhS (Mel-RhS).</p></div><div><h3>Methods</h3><p>LC-OCT and M-Duo Technology® were compared to conventional analysis of the RhS and Mel-RhS microenvironments.</p></div><div><h3>Results</h3><p>LC-OCT successfully visualized the distinct layers of the epidermis and tumor structures within the Mel-RhS, identifying keratinocytes, melanocytes, tumor nests, and fibroblasts. The M-Duo Technology® revealed differences in <em>in situ</em> cytokine (IL-6) and chemokine (CCL2, CXCL10, and IL-8) secretion between Mel-RhS and the control RhS. Notably, such differences were not detected through conventional investigation of secreted proteins in culture supernatants.</p></div><div><h3>Conclusion</h3><p>The combination of LC-OCT's high-resolution imaging and M-Duo Technology®’s <em>in situ</em> microenvironmental mapping has the potential to provide a synergistic platform for non-invasive, real-time analysis, allowing for prolonged observation of processes within Mel-RhS models without the need for culture disruption.</p></div>","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":"115 2","pages":"Pages 85-93"},"PeriodicalIF":4.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923181124001476/pdfft?md5=4a78bb95ef799ad9258a7621626a85d2&pid=1-s2.0-S0923181124001476-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141692014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical application of a BCL-2 inhibitor ameliorates imiquimod-induced psoriasiform dermatitis by eliminating senescent cells","authors":"","doi":"10.1016/j.jdermsci.2024.06.002","DOIUrl":"10.1016/j.jdermsci.2024.06.002","url":null,"abstract":"<div><h3>Background</h3><p>Psoriasis is an inflammatory skin disease with unclear pathogenesis and unmet therapeutic needs.</p></div><div><h3>Objective</h3><p>To investigate the role of senescent CD4⁺<span> T cells in psoriatic lesion formation and explore the application of senolytics in treating psoriasis.</span></p></div><div><h3>Methods</h3><p>We explored the expression levels of p16^INK4a<span> and p21, classical markers of cellular senescence, in CD4</span>⁺ T cells from human psoriatic lesions and imiquimod (IMQ)-induced psoriatic lesions. We prepared a senolytic gel using B-cell lymphoma 2 (BCL-2) inhibitor ABT-737 and evaluated its therapeutic efficacy in treating psoriasis.</p></div><div><h3>Results</h3><p>Using multispectrum immunohistochemistry (mIHC) staining, we detected increased expression levels of p16^INK4a and p21 in CD4⁺<span> T cells from psoriatic lesions. After topical application of ABT-737 gel, significant alleviation of IMQ-induced psoriatic lesions was observed, with milder pathological alterations. Mechanistically, ABT-737 gel significantly decreased the percentage of senescent cells, expression of T cell receptor<span> (TCR) α and β chains, and expression of </span></span><span><em>Tet methylcytosine </em><em>dioxygenase</em><em> 2</em></span> (<em>Tet2</em>) in IMQ-induced psoriatic lesions, as determined by mIHC, high-throughput sequencing of the TCR repertoire, and RT-qPCR, respectively. Furthermore, the severity of psoriatic lesions in CD4^creTet2^f/f mice was milder than that in Tet2^f/f mice in the IMQ-induced psoriasis model.</p></div><div><h3>Conclusion</h3><p>We revealed the roles of senescent CD4⁺ T cells in developing psoriasis and highlighted the therapeutic potential of topical ABT-737 gel in treating psoriasis through the elimination of senescent cells, modulation of the TCR αβ repertoire, and regulation of the TET2-Th17 cell pathway.</p></div>","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":"115 2","pages":"Pages 54-63"},"PeriodicalIF":4.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editors Choice","authors":"","doi":"10.1016/S0923-1811(24)00156-7","DOIUrl":"10.1016/S0923-1811(24)00156-7","url":null,"abstract":"","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":"115 2","pages":"Page 53"},"PeriodicalIF":4.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923181124001567/pdfft?md5=a56e12e719d32213b60726ca0e22cd16&pid=1-s2.0-S0923181124001567-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142040945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-25–5p in exosomes derived from UVB-induced fibroblasts regulates melanogenesis via TSC2-dominated cellular organelle dysfunction","authors":"","doi":"10.1016/j.jdermsci.2024.06.001","DOIUrl":"10.1016/j.jdermsci.2024.06.001","url":null,"abstract":"<div><h3>Background</h3><p><span>Few reports have confirmed whether exosomes derived from fibroblasts can regulate the process of </span>melanogenesis<span>. We wondered whether exosomes<span> derived from fibroblasts could have a potent regulatory effect on melanogenesis and explored the underlying mechanisms.</span></span></p></div><div><h3>Objective</h3><p>This study aimed to find the role of fibroblasts in melanocytes and revealed the related mechanisms.</p></div><div><h3>Methods</h3><p>RT-qPCR, Western blot analysis<span><span><span><span> were conducted to measure the RNA and </span>protein expression level of various related genes. </span>miRNA<span><span> sequencing, mass spectrum analysis and subsequent bioinformatics analysis were employed to find the underlying targets. Zebrafish were employed to measure the </span>melanin synthesis related process in vivo. Furthermore, </span></span>electron microscopy<span>, ROS measurement and dual-luciferase reporter assay were adopted to investigate the relationship between these processes.</span></span></p></div><div><h3>Results</h3><p><span><span><span>We found that exosomes derived from human primary dermal fibroblasts were internalized by human primary </span>melanocytes<span> and MNT1 cells and that the melanin<span> content and the expression of melanin synthesis-related proteins TYR and </span></span></span>MITF<span><span> was inhibited by exosomes derived from UVB-induced human primary dermal fibroblasts. The miRNA expression profile in secreted exosomes changed significantly, with miR-25–5p identified as capable of regulating </span>TSC2<span> expression via the CDS region. The miR-25–5p-TSC2 axis could affect the melanin content through subsequent cellular organelle dysfunction, such as mitochondrial dysfunction, </span></span></span>endoplasmic reticulum stress<span> and dysregulation of lysosomal cysteine proteases.</span></p></div><div><h3>Conclusion</h3><p>We unveiled a novel regulatory role of fibroblasts in melanocytes<span>, facilitated by the secretion of exosomes. miR-25–5p within exosomes plays a pivotal role in regulating melanogenesis via TSC2-induced cellular organelle dysfunction.</span></p></div>","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":"115 2","pages":"Pages 75-84"},"PeriodicalIF":4.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141408728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Necrosulfonamide promotes hair growth and ameliorates DHT-induced hair growth inhibition","authors":"Yuanhong Liu ,&nbsp;Shengbo Yang ,&nbsp;Lina Tan ,&nbsp;Xuemei Li,&nbsp;Daijing Long,&nbsp;Jianyun Lu,&nbsp;Dan Wang","doi":"10.1016/j.jdermsci.2024.04.004","DOIUrl":"10.1016/j.jdermsci.2024.04.004","url":null,"abstract":"<div><h3>Background</h3><p><span>Alopecia<span> affects patients' appearance and psychology. Mixed-lineage kinase domain-like pseudokinase (MLKL)-mediated </span></span>necroptosis<span> plays a role in various skin diseases, but its effect on hair growth is unclear.</span></p></div><div><h3>Objective</h3><p>To investigate the effects of MLKL on hair growth and its regulatory mechanisms and to determine the potential clinical value of Necrosulfonamide (NSA, a MLKL-targeting inhibitor) in promoting hair growth and counteracting dihydrotestosterone (DHT) inhibition of hair growth.</p></div><div><h3>Methods</h3><p><span>The expression level of MLKL was detected in the scalp of </span>androgenetic alopecia (AGA) patients and the skin tissues of mice. Knock down MLKL expression or use NSA to observe hair growth in vivo and in vitro.</p></div><div><h3>Results</h3><p><span>In AGA patients, MLKL expression is elevated in the alopecia areas. In mice, MLKL is significantly expressed in the outer root sheath (ORS) cells of </span>hair follicles<span><span>, peaking during the catagen phase. Knockdown expression of MLKL in mice skin promoted hair growth. NSA enhanced hair growth and prevented hair follicle regression via the Wnt signaling. Reduced MLKL boosts ORS </span>cell proliferation without directly impacting DPCs' growth. Interestingly, NSA boosts DPCs' proliferation and induction when co-cultured with ORS cells. Besides, NSA alleviated the inhibition of DHT on hair growth in vivo and vitro.</span></p></div><div><h3>Conclusion</h3><p>NSA inhibited the activation of MLKL in ORS cells, promoted the activation of Wnt signal in DPC cells, and improved the inhibition of hair growth by DHT, illuminating a new alopecia mechanism and aiding anti-alopecia drug development.</p></div>","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":"115 2","pages":"Pages 64-74"},"PeriodicalIF":4.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141753673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"JSID2024-1","authors":"","doi":"10.1016/S0923-1811(24)00154-3","DOIUrl":"10.1016/S0923-1811(24)00154-3","url":null,"abstract":"","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":"115 2","pages":"Page I"},"PeriodicalIF":4.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}