Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture最新文献

{"title":"Targeting the dependence on PIK3C3-mTORC1 signaling in dormancy-prone breast cancer cells blunts metastasis initiation.","authors":"Islam E Elkholi, Amélie Robert, Camille Malouf, Hellen Kuasne, Stanislav Drapela, Graham Macleod, Steven Hébert, Alain Pacis, Virginie Calderon, Claudia L Kleinman, Ana P Gomes, Julio A Aguirre-Ghiso, Morag Park, Stéphane Angers, Jean-François Côté","doi":"10.1101/2023.08.02.551681","DOIUrl":"10.1101/2023.08.02.551681","url":null,"abstract":"<p><p>Halting breast cancer metastatic relapses following primary tumor removal and the clinical dormant phase, remains challenging, due to a lack of specific vulnerabilities to target during dormancy. To address this, we conducted genome-wide CRISPR screens on two breast cancer cell lines with distinct dormancy properties: 4T1 (short-term dormancy) and 4T07 (prolonged dormancy). We discovered that loss of class-III PI3K, Pik3c3, revealed a unique vulnerability in 4T07 cells. Surprisingly, dormancy-prone 4T07 cells exhibited higher mTORC1 activity than 4T1 cells, due to lysosome-dependent signaling occurring at the cell periphery. Pharmacological inhibition of Pik3c3 counteracted this phenotype in 4T07 cells, and selectively reduced metastasis burden only in the 4T07 dormancy-prone model. This mechanism was also detected in human breast cancer cell lines in addition to a breast cancer patient-derived xenograft supporting that it may be relevant in humans. Our findings suggest dormant cancer cell-initiated metastasis may be prevented in patients carrying tumor cells that display PIK3C3-peripheral lysosomal signaling to mTORC1.</p><p><strong>Statement of significance: </strong>We reveal that dormancy-prone breast cancer cells depend on the class III PI3K to mediate a constant peripheral lysosomal positioning and mTORC1 hyperactivity. Targeting this pathway might blunt breast cancer metastasis.</p>","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11360912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89559233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of wearing textured versus smooth shoe insoles for 12 weeks on gait, foot sensation and patient-reported outcomes, in people with multiple sclerosis: a randomised controlled trial.","authors":"Anna L Hatton, Katrina Williams, Mark D Chatfield, Sheree Hurn, Jayishni N Maharaj, Elise M Gane, Thomas Cattagni, John Dixon, Keith Rome, Graham Kerr, Sandra G Brauer","doi":"10.1017/BrImp.2022.33","DOIUrl":"10.1017/BrImp.2022.33","url":null,"abstract":"<p><strong>Background: </strong>Innovative shoe insoles, designed to enhance sensory information on the plantar surface of the feet, could help to improve walking in people with Multiple Sclerosis.</p><p><strong>Objective: </strong>To compare the effects of wearing textured versus smooth insoles, on measures of gait, foot sensation and patient-reported outcomes, in people with Multiple Sclerosis.</p><p><strong>Methods: </strong>A prospective, randomised controlled trial was conducted with concealed allocation, assessor blinding and intention-to-treat analysis. Thirty ambulant men and women with multiple sclerosis (MS) (Disease Steps rating 1-4) were randomly allocated to wear textured or smooth insoles for 12 weeks. Self-reported insole wear and falls diaries were completed over the intervention period. Laboratory assessments of spatiotemporal gait patterns, foot sensation and proprioception, and patient-reported outcomes, were performed at Weeks 0 (Baseline 1), 4 (Baseline 2) and 16 (Post-Intervention). The primary outcome was the size of the mediolateral base of support (stride/step width) when walking over even and uneven surfaces. Independent <i>t</i>-tests were performed on change from baseline (average of baseline measures) to post-intervention.</p><p><strong>Results: </strong>There were no differences in stride width between groups, when walking over the even or uneven surfaces (<i>P</i> ≥ 0.20) at post-intervention. There were no between-group differences for any secondary outcomes including gait (all <i>P</i> values > 0.23), foot sensory function (all <i>P</i> values ≥ 0.08) and patient-reported outcomes (all <i>P</i> values ≥ 0.23).</p><p><strong>Conclusions: </strong>In our small trial, prolonged wear of textured insoles did not appear to alter walking or foot sensation in people with MS who have limited foot sensory loss. Further investigation is needed to explore optimal insole design.</p><p><strong>Clinical trial registration: </strong>Australian and New Zealand Clinical Trials Registry (ACTRN12615000421538).</p>","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"72 1","pages":"148-167"},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89537874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What We Have Learned About Combining a Ketogenic Diet and Chemoimmunotherapy: a Case Report and Review of Literature.","authors":"Daniel Sims, Agnes K Liman, Victoria Leung, Andrew Hwang, Jeffrey Means, Andrew D Liman","doi":"10.12788/fp.0399","DOIUrl":"10.12788/fp.0399","url":null,"abstract":"<p><strong>Background: </strong>A high-fat, moderate-protein, low-carbohydrate ketogenic diet has been reported in the literature as a treatment option for patients with cancer.</p><p><strong>Case presentation: </strong>A 69-year-old veteran was initially diagnosed with stage III colorectal cancer and progressed to having liver, pancreatic, and omental lymph node involvement despite completing adjuvant FOLFOX (fluorouracil, leucovorin calcium, and oxaliplatin) after surgery. The patient was treated with FOLFIRI (fluorouracil, leucovorin calcium, and irinotecan hydrochloride) and bevacizumab, followed by encorafenib and cetuximab on progression. Subsequently, he received pembrolizumab but continued to progress. The patient was later placed on trifluridine/tipiracil and bevacizumab concurrent with a ketogenic diet. Positron emission tomography and carcinoembryonic antigen levels indicated disease stabilization for 10 months. On progression, the patient was transitioned to ipilumimab and nivolumab and continued to adhere to the ketogenic diet. The patient's disease has continued to remain stable for the past 1 year. His degree of ketosis was determined using the glucose ketone index. The patient continues to have a good quality of life during concurrent ketogenic diet and therapy.</p><p><strong>Conclusions: </strong>This case supports the tolerability of the ketogenic diet along with chemotherapy and immunotherapy and should be considered as an adjunct to standard cancer treatment. In this report, we reviewed the latest literature about cellular mechanism of the ketogenic diet and the efficacy and relationship with chemotherapy and immunotherapy. We are about to open a ketogenic diet protocol at the Veterans Affairs Central California Health Care System in Fresno.</p>","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"70 1","pages":"S98-S104"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10681018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89522355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of some Compounds Found in Aronia and Goji Berries on Human Health","authors":"Anca Maria Chiorean, E. Buta, V. Mitre","doi":"10.15835/buasvmcn-hort:2021.0033","DOIUrl":"https://doi.org/10.15835/buasvmcn-hort:2021.0033","url":null,"abstract":"Berries consumption is a current concern in order to highlight the important content of their compounds on human health. Aronia berry is known as chokeberry and is native in North America and Canada. Due to the therapeutic properties, black chokeberries are very popular and appreciated fruits. The most important compounds, polyphenols and antioxidants, have many biological actions such as antioxidative, anti-inflammatory, hypotensive, antiviral, anticancer, antidiabetic and antiatherosclerotic effects Goji berry is also another important source of carotenoids and antioxidants and it has been used as a medicine in China for centuries. Goji berry is considered as a super fruit due to bioactive compounds that offer protection against cardiovascular diseases, diabetes and other comorbidities. Black chokeberry and goji berry have an antioxidant capacity ten times higher than other berries. Due to their high biological and nutritional value, these berries are being used more and more frequently in human nutrition and the extracts in the pharmaceutical industry. Therefore, the main aim of this paper was to summarize and highlight the high content of bioactive compounds and beneficial effects of aronia and goji berries upon human health.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78811044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Substrate Types and Bio-phyto-modulators on Spruce Seed Germination and Seedling Development (Picea abies [L.] Karst.)","authors":"Steluța Sîngeorzan, I. Păcurar, Irina M. Morar, A. Truță, C. Albert, H. Criveanu, L. Holonec","doi":"10.15835/buasvmcn-hort:2022.0007","DOIUrl":"https://doi.org/10.15835/buasvmcn-hort:2022.0007","url":null,"abstract":"Spruce is a European native coniferous species, spreading over the area in between The Carpathian Mountains and the Alps, up to the northern parts of the Scandinavian Peninsula. In the future, spruce forests will have to cope with the growing climate change that is already threatening their natural habitats. This study has focused on the spruce seed germination process and seedlings growth under the influence of DEA (Water Energizing Device) bio-phyto-modulators. The biological material under analysis consists of spruce seeds selected from four Romanian sources included in the National Catalogue of Basic Materials for Generating Forestry Reproduction Material. Five different substrates were used for tasting germination capacity, with two different treatments (distilled water and energized water). Analyzing the treatments used it can be notice that subjecting the seeds to energized water treatment led to a higher germination capacity than in the case of those watered with distilled water (40-94%) and, respectively (24-84%). The seedlings growth was also higher in the case of those springing from the energized water treated seeds (2.45-4.23 cm) than from those springing from the distilled water treated seeds (2.07 cm-3.14 cm). The differences in germination capacity, growth and development were observed for all the provenances. Applying DEA bio-phyto-modulators has proven beneficial, with noticeable differences for both germination capacity and seedlings growth.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83877840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An apparent breakdown of Vf resistance occurring in an apple orchard in Bistrita area","authors":"S. Roșu-Mareș, G. Guzu, I. Zagrai, C. Puia","doi":"10.15835/buasvmcn-hort:2021.0032","DOIUrl":"https://doi.org/10.15835/buasvmcn-hort:2021.0032","url":null,"abstract":"Scab resistance through the Rvi6 gene was largely used in Romanian breeding programs including those from Fruit Research & Development Station (FRDS) Bistrita. The aim of the study was to verify the efficacy of the resistance due to the Vf gene under the current conditions in the Bistrita area. Three scab resistant apple cultivars bred at FRDS Bistrita were monitored in the field, in comparison with the Rvi6 ‘Florina’ and ‘Prima’ cvs., and the sensitive ‘Idared’ and ‘Golden Delicious’ cvs. Molecular markers (AM-19, U1₄₀₀ and AL07) were used in PCR to check the presence of the Rvi6 gene in the genome of the three Romanian varieties. During the years 2019-2021, scab lesions appeared on all the resistant cultivars studied. PCR results showed the presence of Rvi6 gene on ‘Bistritean’, ‘Salva’, ‘Aura’, ‘Prima’ and ‘Florina’ genoms. The results suggest a possible breakdown of the resistance given by the Rvi6 gene in the current ecological conditions in this area. Scab symptoms occurring on cultivars that have the Rvi6 gene could lead to the hypothesis that different scab races are now present in our area or that some genetic variations occurred on the existing races so that they overcome Rvi6 gene.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79016812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Pollen Grain Characterization of Several Apple and Plum Varieties","authors":"Vlad Popa, L. Bădulescu, M. Iordachescu, A. Udriște","doi":"10.15835/buasvmcn-hort:2021.0020","DOIUrl":"https://doi.org/10.15835/buasvmcn-hort:2021.0020","url":null,"abstract":"Palynology is one of the most used methods to determine the species of different plants. It acts like a fingerprint, being unique to each plant species. The aim of this paper was to observe differences both at morphological level and from the pollen viability point of view of 21 apple (Malus domestica Borkh.) and 19 plum (Prunus domestica L.) varieties, differences that can be correlated with genotype variation in further genotyping research, with the final goal of using these results as a basis for genotype-assisted breeding programs. Pollen grain (PG) morphology was assessed using a Scanning Electron Microscope and pollen viability was analyzed using in vitro germination. This study demonstrated significant differences in both the size of PGs and the percentage of viable pollen through in vitro germination. Among the apple varieties, Belle de Boskoop presented the largest PGs, and among the plum varieties Timpurii de Țurlești. Aura apple variety had the lowest viability percentage.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"321 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76289767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Cover Crops to Control Arthropod Pests in Organic Viticulture","authors":"A. Ranca, Anne-Laure Fragnière, Sergiu-Ayar Ene, Š. Modic, Vittorio Rossi, T. Caffi, J. Razinger, Eugenia RODRIGO SANTAMALIA, D. Wipf","doi":"10.15835/buasvmcn-hort:2021.0041","DOIUrl":"https://doi.org/10.15835/buasvmcn-hort:2021.0041","url":null,"abstract":"Plant diversity has the potential to conserve beneficials and thereby naturally controlling arthropod pests. Beneficials’ activity can be increased by pollen-rich plant species. Here we aimed to develop innovative viticultural systems that naturally control arthropod pests, by increasing plant diversity within vineyards planting of selected cover crops. The experimental vineyards were set-up in Chablis (France), Piacenza (Italy), Murfatlar (Romania), Manče (Slovenia), Valencia (Spain) and Nyon (Switzerland). Each vineyard was divided in a traditional and innovative subplot and monitored in 2019 and 2020. The effect of cover crops on arthropods was assessed according to a common protocol using visual samplings and specific traps. Analysing the obtained data, there were no statistically significant differences in the abundance of sampled arthropod pests in the innovative and the traditional systems. Yet, two of five studied pest species tended to be less abundant in the innovative systems. Regarding arthropod beneficials, flying parasitoids and bees tended to benefit from the planting of cover crops in the innovative compared to the traditional systems. Alike, predators such as spiders and carabids were more abundant in vineyards with understorey vegetation. Overall, the arthropod biodiversity seems to benefit from the planting of cover crops but without necessarily favouring pest suppression.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76830398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Gis Study about Forest Management in Strâmbu Băiuț","authors":"M. Nap, Diana Ficior, T. Sălăgean, I. Pop, F. Matei, I. Coroian, J. Deak, Silvia Chiorean, E. Șuba","doi":"10.15835/buasvmcn-hort:2021.0025","DOIUrl":"https://doi.org/10.15835/buasvmcn-hort:2021.0025","url":null,"abstract":"Forests have a major role in preserving an environment, suitable for human life, being a very important and renewable natural resource. Because of the multiple interests of forest users, a wider range of up-to-date information is being requested in the community of forestry. The study describes a geographic information system (GIS) research, based on information about a protected forest area. The aim of this paper is to develop a management plan that lays the foundations for a long-term management of natural resources, both in favor of humans, and nature, to promote the values of biodiversity in this protected natural area, involving as much as possible, the communities in the area. GIS can be used not only to edit and display maps, but also to enhance work quality. These enhancements include an exploration of information from different types of maps, recognizing potentially problematic areas, facilitating informative public hearings, and presenting major area features. GIS has been recently applied to numerous fields, including forestry. Most GIS research works on land-use planning and forest management, relates to the establishment of databases, the expansion of mathematical models to incorporate the capabilities of spatial analyses. The results of this study reveal several features including: classification of plots by altitude, slope, exhibition, consistency of the tree, dominant species, accessibility of the plots, and identifying the areas where the snow lasts the longest. GIS technology provides users easier access to various information’s about forest features and to visualize them through the maps. The management of this database facilitates the implementation of methods of conservation of protected forests.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80658717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and Biochemical Characterisation of the Newly Developed Cultivar of Momordica charantia – Brâncuşi","authors":"C. Vinatoru, B. Mușat, C. Bratu, G. Negoșanu, Matilda Popescu, Cristian Şomoiag","doi":"10.15835/buasvmcn-hort:2021.0027","DOIUrl":"https://doi.org/10.15835/buasvmcn-hort:2021.0027","url":null,"abstract":"Our study aimed to carry out a phenotypic and biochemical characterization of the newly-developed cv. of Momordica charantia namely BRÂNCUȘI. Momordica charantia contains biologically active plant chemicals as confirmed by the latest studies. Our germplasm collection consists of 30 varieties, from which BRÂNCUŞI variety was bred by repeated individual selection. The working methods consisted in phenotypic and biometrical observations. The biochemical analyses were performed by organoleptic and physico chemical examinations of 3 variants of processed fruit: powder, paste and dried fruit. BRÂNCUŞI variety presents a distinct phenotypic expressiveness with white immature fruit and the highest yield per plant, of 9.2 kg. The highest percentage of total invert sugar, 1.18 % but also carbohydrates 69.4 % was obtained in the fine powder variant, which outlines a specific direction of use with numerous benefits in human nutrition and health. BRÂNCUŞI variety has distinct phenotypic characteristics that confer uniqueness compared to other varieties.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75806132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}