Hematology, transfusion and cell therapy最新文献

{"title":"Coagulation factor stability and sterility of thawed fresh frozen plasma stored at 2-6 ^o C for five days: Towards optimizing utilization.","authors":"Ruchika Bhartia, Paramjit Kaur, Kshitija Mittal, Anita Tahlan, Varsha Gupta, Ravneet Kaur, Gagandeep Kaur","doi":"10.1016/j.htct.2025.103988","DOIUrl":"https://doi.org/10.1016/j.htct.2025.103988","url":null,"abstract":"<p><strong>Background: </strong>Fresh frozen plasma plays a crucial role in managing trauma and bleeding patients. The concern about a decline in labile coagulation factors limits its usage beyond 24 hours. This study aimed to analyze coagulation factor levels and microbial contamination of thawed fresh frozen plasma stored at 2-6 °C for five days.</p><p><strong>Material and methods: </strong>A prospective observational study was conducted on 40 male donors with blood groups A and O selected through purposive sampling. Blood was collected in 450 mL bags and freshly prepared plasma was aliquoted and frozen at -80 °C. Aliquots were thawed at 37 °C and tested on Days 0, 1, and 5 after storage at 2-6 °C. Coagulation screening assays and activity of coagulation factors V, VIII, IX, fibrinogen, and von Willebrand factor were performed. Samples were tested for sterility on Day 5.</p><p><strong>Results: </strong>One-way ANOVA revealed a significant increase in mean prothrombin time, activated partial thromboplastin time, and international normalized ratio during storage (p-value < 0.001). The activity of factors V and VIII showed a significant decrease over five days (factor V - 20.0 % and factor VIII - 42.2 %; p-value < 0.001), with factor VIII activity declining by 30.8 % within the first 24 hours and remaining relatively stable thereafter. Mean von Willebrand factor activity was lower in fresh frozen plasma from O blood group donors (p-value < 0.05) on Days 1 and 5 of storage using an unpaired t-test. Cultures were sterile on Day 5.</p><p><strong>Conclusion: </strong>Key coagulation factors were well preserved in thawed plasma till five days of storage at 2-6 °C without compromising product sterility suggesting potential for extended shelf life.</p>","PeriodicalId":94026,"journal":{"name":"Hematology, transfusion and cell therapy","volume":"47 4","pages":"103988"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145260310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of serum interleukin-8 with disease severity in aplastic anemia.","authors":"Shahla'a Fadhil Sabir, Huda Ibraheem Abd Al-Lateef, Naam Ali Hamza","doi":"10.1016/j.htct.2025.103989","DOIUrl":"https://doi.org/10.1016/j.htct.2025.103989","url":null,"abstract":"<p><strong>Background: </strong>Aplastic anemia is a heterogeneous group of hematological disorders identified by the presence of one or more cytopenias in the bone marrow or the peripheral blood or both, affecting either one or multiple blood cell lineages. The pathophysiology of this disorder is unclear; however, the prevailing hypothesis posits that an aberrant immune response is responsible for the death of hematopoietic precursor stem cells due to autoreactive cytotoxic T lymphocytes in individuals with a genetic predisposition. Interleukin-8 acts as a pleiotropic prototype chemokine and serves as a powerful inhibitor of myelopoiesis and assumes an essential role in both the initiation and progression of acute inflammation and tissue damage. Consequently, it is postulated that the sustained elevation in interleukin-8 production could potentially lead to immune-mediated bone marrow failure in aplastic anemia. The aim of this study was to evaluate the serum interleukin-8 concentrations in individuals with aplastic anemia as well as investigate its potential connection with disease severity.</p><p><strong>Methods: </strong>This study was performed at the National Center of Hematology and included 28 aplastic anemia patients and 30 healthy individuals matched by age and gender as a control group. The serum interleukin-8 levels were measured by the quantitative sandwich enzyme-linked immunosorbent assay (ELISA) method.</p><p><strong>Results: </strong>Considerrable elevation in serum interleukin-8 levels were observed between the two groups (p-value = 0.405). Of the aplastic anemia patients, severe cases had significantly higher levels of interleukin-8 compared to non-severe patients (p-value = 0.0495), with a cutoff serum level of 7.71 pg/mL.</p><p><strong>Conclusion: </strong>Interleukin-8 may have a role in the immune-mediated pathophysiology of aplastic anemia as well as have a significant correlation with disease severity.</p>","PeriodicalId":94026,"journal":{"name":"Hematology, transfusion and cell therapy","volume":"47 4","pages":"103989"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145260359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experience with second allogeneic hematopoietic stem cell transplantation in Chilean patients: A single-center study.","authors":"Yorman Flores, Javier Díaz, Patricio Rojas, José Salinas, Catherine Gutiérrez, Marcela Vidal, Verónica Jara, Elizabeth Rivera, María José García, Vicente Sandoval, Felipe Palacios, Maximiliano Vergara, Mauricio Ocqueteau, Mauricio Sarmiento","doi":"10.1016/j.htct.2025.103980","DOIUrl":"https://doi.org/10.1016/j.htct.2025.103980","url":null,"abstract":"<p><strong>Introduction: </strong>Allogeneic hematopoietic stem cell transplantation is potentially a curative treatment for several hematological diseases. However, post-transplant relapse remains a significant challenge. For patients who achieve a second complete remission, a second allogeneic transplantation may be a promising therapeutic option. The aim of this study was to analyze clinical outcomes including graft-versus-host disease, non-relapse mortality, and relapse rates, as well as graft sources in patients who underwent a second allogeneic transplantation in a university-based transplant program.</p><p><strong>Patients and methods: </strong>A retrospective analysis of 21 adult patients who underwent a second allogeneic transplantation between 2001 and 2023 was performed. Data on demographics, underlying disease, graft source, conditioning, graft-versus-host disease, relapse, and survival were collected. Survival estimates were calculated using the Kaplan-Meier method.</p><p><strong>Results: </strong>The graft source was bone marrow in 60 % and peripheral blood in 40 % of cases. Grade III-IV acute graft-versus-host disease occurred in 5 % and extensive chronic graft-versus-host disease in 17 %. The non-relapse mortality was 69.2 %, and disease relapse occurred in 23.1 %. The one-year progression-free survival was 26.5 %, and overall survival was 42.3 %. Compared to those transplanted before 2010, patients who underwent transplantation after 2010 showed improved two-year PFS and OS, reaching 55 % and 45.4 %, respectively.</p><p><strong>Conclusion: </strong>A second allogeneic transplantation may offer a survival benefit in selected patients with relapsed hematologic malignancies or bone marrow failure syndromes. Despite high non-relapse mortality, outcomes have improved in recent years with better salvage strategies.</p>","PeriodicalId":94026,"journal":{"name":"Hematology, transfusion and cell therapy","volume":"47 4","pages":"103980"},"PeriodicalIF":0.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concurrent intravenous immunoglobulin and platelet transfusion for refractory alloimmune thrombocytopenia in patients undergoing allogeneic hematopoietic stem cell transplantation.","authors":"Moazzam Shahzad, Muhammad Kashif Amin, Maggie Nelson, Abhinav Vyas, Joe S Al-Ramahi, Nausheen Ahmed, Rajat Bansal, Haitham Abdelhakim, Leyla Shune, Al-Ola Abdallah, Anurag K Singh, Sunil H Abhyankar, Joseph P McGuirk, Muhammad Umair Mushtaq","doi":"10.1016/j.htct.2025.103961","DOIUrl":"10.1016/j.htct.2025.103961","url":null,"abstract":"<p><strong>Background: </strong>Severe refractory alloimmune thrombocytopenia is a challenging and life-threatening complication in patients with hematologic disorders who are undergoing allogeneic hematopoietic stem cell transplantation. This study aimed to investigate the utility of continuous intravenous immunoglobulin and platelet transfusions as a therapeutic approach for alloimmune thrombocytopenia in patients undergoing allogeneic transplants.</p><p><strong>Methods: </strong>A single-center retrospective analysis was conducted of ten adult allogeneic transplant patients hospitalized with transfusion-refractory alloimmune thrombocytopenia. Intravenous immunoglobulin (2 g/kg) was administered as a slow continuous infusion over 48 h along with a continuous apheresis platelet infusion (one apheresis unit over eight hours). Clinical response was defined as the resolution of bleeding or patients being able to undergo the required procedure without bleeding complications.</p><p><strong>Results: </strong>The median time after the transplant was 27.5 (range: 7-299) days. Myeloablative and reduced-intensity conditioning were performed in 5 (50 %) and 5 (50 %) patients, respectively. The median platelet count at the time of infusion was 4.5 × 10⁹/L. All patients were able to achieve clinical response with the median maximum platelet count within ten days of the infusion being 41.0 × 10⁹/L. The median time to best response was three days with a median platelet count of 27.0 × 10⁹/L.</p><p><strong>Conclusions: </strong>Continuous intravenous immunoglobulin and platelet infusions over 48 h may be able to overcome life-threatening refractory alloimmune thrombocytopenia in transplant patients and may provide a bridging measure until platelet engraftment or for life-threatening hemorrhage or invasive procedures with high bleeding risk.</p>","PeriodicalId":94026,"journal":{"name":"Hematology, transfusion and cell therapy","volume":"47 4","pages":"103961"},"PeriodicalIF":0.0,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12356998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144823486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The battle against pediatric retinoblastoma: insights from 2024 medical literature.","authors":"Yoshiyasu Takefuji","doi":"10.1016/j.htct.2025.103839","DOIUrl":"10.1016/j.htct.2025.103839","url":null,"abstract":"","PeriodicalId":94026,"journal":{"name":"Hematology, transfusion and cell therapy","volume":"47 4","pages":"103839"},"PeriodicalIF":0.0,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of hydroxyurea on tubular phosphate handling in sickle cell nephropathy","authors":"Gabriela Araujo de Abreu, Duaran Lopes de Sousa, Suzzy Maria Carvalho Dantas, Alice Maria Costa Martins, T. L. Sampaio, R. Lemes","doi":"10.1016/j.htct.2023.11.015","DOIUrl":"https://doi.org/10.1016/j.htct.2023.11.015","url":null,"abstract":"","PeriodicalId":94026,"journal":{"name":"Hematology, transfusion and cell therapy","volume":"16 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139818365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemorrhagic code protocol, a successful case in the patient blood management model for patients with severe hemorrhages","authors":"João Carlos de Campos Guerra, Michele Jaures, Roseny dos Reis Rodrigues, A. Cypriano, D. Malheiro, Anna Carolina Batista Dantas, Fernanda Paulino Fernandes, Neila Maria Marques Negrini, V. Teich","doi":"10.1016/j.htct.2024.01.002","DOIUrl":"https://doi.org/10.1016/j.htct.2024.01.002","url":null,"abstract":"","PeriodicalId":94026,"journal":{"name":"Hematology, transfusion and cell therapy","volume":"34 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139826614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HLA-DR-DQ associations, combined with PLASMIC score, are reliable predictors of acquired thrombotic thrombocytopenic purpura (aTTP) and aid in differentiating aTTP from other thrombotic microangiopathies","authors":"Soumya Pandey, Akul Shrivastava, Y. Harville, Michele Cottler-Fox, T. Harville","doi":"10.1016/j.htct.2023.11.016","DOIUrl":"https://doi.org/10.1016/j.htct.2023.11.016","url":null,"abstract":"","PeriodicalId":94026,"journal":{"name":"Hematology, transfusion and cell therapy","volume":"31 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139888795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-PF4 antibodies and their relationship with COVID infection","authors":"Chieh Yang, Irene Wang, Akshit Chitkara, Jibin Swankutty, Rushin Patel, Samir V Kubba","doi":"10.1016/j.htct.2023.11.012","DOIUrl":"https://doi.org/10.1016/j.htct.2023.11.012","url":null,"abstract":"","PeriodicalId":94026,"journal":{"name":"Hematology, transfusion and cell therapy","volume":"31 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139892698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology, patient journey and unmet needs related to hemophilia in Brazil: a scoping review with evidence map","authors":"N. B. Schneider, C. L. P. de Araujo, Harryson Wings Godoy dos Santos, Simone Lima, Maicon Falavigna, D. Pachito","doi":"10.1016/j.htct.2023.12.004","DOIUrl":"https://doi.org/10.1016/j.htct.2023.12.004","url":null,"abstract":"","PeriodicalId":94026,"journal":{"name":"Hematology, transfusion and cell therapy","volume":"525 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139831674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}