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[Evaluation of Salt Tolerance of Transgenic Tobacco Plants Bearing with P5CS1 Gene of Arabidopsis thaliana]. [评价带有拟南芥 P5CS1 基因的转基因烟草植株的耐盐性]。
Genetika Pub Date : 2015-12-01
S M Ibragimova, E A Trifonova, E A Filipenko, V K Shymny
{"title":"[Evaluation of Salt Tolerance of Transgenic Tobacco Plants Bearing with P5CS1 Gene of Arabidopsis thaliana].","authors":"S M Ibragimova, E A Trifonova, E A Filipenko, V K Shymny","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Arabidopsis thaliana delta1-pyrroline-5-carhoxylate synthase 1 gene (P5CS1) cDNA was cloned under the control of the potent constitutive 35S RNA promoter of the cauliflower mosaic virus and transferred into genome of tobacco cv. Petit Havana SR-1 (Nicotiana tabacum L.) plants. It is shown that the constitutive level of proline in the transgenic plants T0 exceeds that of the SR1 reference line by 1.5 to 4 times. Under conditions of salt stress (200, 300 mM NaCl) T1-generation transgenic plants in early stages of development formed a large biomass, developed more quickly, and had a higher rate of root growth compared to the control, which confirms the involvement of the P5CS1 gene in molecular mechanisms of stress resistance in plants.</p>","PeriodicalId":94019,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Analysis of Phenotypic Manifestation of peanut Gene Expression Suppression by RNAi in Drosophila Oogenesis]. 【RNAi抑制果蝇卵子发生过程中花生基因表达的表型表现分析】。
Genetika Pub Date : 2015-09-01
K A Akhmetova, C N Dorogova, I N Chesnokov, S A Fedorova
{"title":"[Analysis of Phenotypic Manifestation of peanut Gene Expression Suppression by RNAi in Drosophila Oogenesis].","authors":"K A Akhmetova,&nbsp;C N Dorogova,&nbsp;I N Chesnokov,&nbsp;S A Fedorova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The peanut gene functions in Drosophila melanogaster oogenesis were studied. It was demonstrated that the suppression of peanut expression by RNA interference in the ovary follicular cells results in the violation of oocyte polarization, anomalous cytokinesis in the chorion cells, and violation of the chromatin condensation in follicular cells. No oogenesis violations were observed in females with decreased peanut gene expression or an absence of the Pnut protein in the ovary generative cells. However, embryos produced by such females had a decreased survival rate caused by two death peaks.</p>","PeriodicalId":94019,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027749/pdf/nihms975443.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41175512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Transgenic tobacco (Nicotiana tabacum SR1) plants expressing the gene coding for Serratia marcescens nuclease]. [表达 Serratia marcescens 核酸酶编码基因的转基因烟草（Nicotiana tabacum SR1）植物]。
Genetika Pub Date : 2002-02-01
E A Trifonova, M L Komarova, O A Syrnik, A V Kochetov, V K Shumnyĭ
{"title":"[Transgenic tobacco (Nicotiana tabacum SR1) plants expressing the gene coding for Serratia marcescens nuclease].","authors":"E A Trifonova, M L Komarova, O A Syrnik, A V Kochetov, V K Shumnyĭ","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The gene coding for the secreted Serratia marcescens endonuclease was fused with the mannopine synthase promoter of Agrobacterium tumefaciens Ti plasmid and transferred to Nicotiana tabacum SR1 plants. The promoter is leaf- and root-specific. The resulting transgenic plants demonstrated elevated nuclease activity. The level of the transgene product was determined in the transgenic lines.</p>","PeriodicalId":94019,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Level of phytohormones in various types of symbiotic pea mutants]. [各种共生豌豆突变体的植物激素水平]。
Genetika Pub Date : 2001-11-01
A V Kholodar', K K Sidorova, V K Shumnyĭ
{"title":"[Level of phytohormones in various types of symbiotic pea mutants].","authors":"A V Kholodar', K K Sidorova, V K Shumnyĭ","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The levels of the phytohormones auxin and gibberellin were studied in the original pea (Pisum sativum L.) cultivars Rondo and Ramonskii 77 and in different types of symbiotic mutants (non-nodulating, with single nodules, and supernodulating) induced from them. The results obtained indicated that the levels of the phytohormones in the symbiotic mutants depend on the plant's genotype, developmental phase, and infection with rhizobia. Two mutants were isolated whose phytohormonal statuses markedly differed from the original forms. These mutants may be used for identification of the genes that determine the auxin and gibberellin statuses.</p>","PeriodicalId":94019,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Molecular-genetic identification and certification of soybean (Glycine max L.) cultivars]. [大豆（Glycine max L. ）栽培品种的分子遗传鉴定和认证]。
Genetika Pub Date : 2001-09-01
A F Brik, Iu M Sivolan
{"title":"[Molecular-genetic identification and certification of soybean (Glycine max L.) cultivars].","authors":"A F Brik, Iu M Sivolan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An approach to certification of soybean genotypes has been developed. The procedure employs three methods of DNA analysis based on polymerase chain reaction (PCR): PCR with arbitrary primers (AP PCR), simple sequence repeat polymorphism (SSRP) analysis, and inter-simple sequence repeat (ISSR) analysis. The approach to certification proposed may be used in both genetic and breeding research and seed production. A \"certificate\" form that reflects the unique characteristics of each cultivar studied is proposed. The results of molecular genetic analysis of allele distribution in genotypes of soybean from different ecological geographic zones permit estimation of the adaptive significance of individual alleles.</p>","PeriodicalId":94019,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Transgenic Tobacco plants expressing bacterial genes encoded the thermostable glucanases]. [转基因烟草植物表达细菌基因编码的恒温葡聚糖酶]。
Genetika Pub Date : 2001-06-01
N R Movsesian, X Alizade, K A Musiĭchuk, Iu G Popov, E S Piruzian
{"title":"[Transgenic Tobacco plants expressing bacterial genes encoded the thermostable glucanases].","authors":"N R Movsesian, X Alizade, K A Musiĭchuk, Iu G Popov, E S Piruzian","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It is shown that bacterial genes for thermostable beta-glucanases are expressed retaining their activity and substrate specificity. The leader peptide of the carrot extensin exerts effective secretion of the bacterial enzymes into the intercellular space of the plant tissue. Expression of the bacterial gene for beta-1,3-glucanase in plant tissues alters their morphogenetic potential. Regeneration of shoots from the calli of these plant lines requires a six- to eightfold increase in cytokinin (6-BAP) concentration in comparison with the control lines and the transgenic lines expressing beta-1,3-1,4-glucanase. Rooting of transgenic plants expressing the bacterial gene for beta-1,3-glucanase occurs much faster. The transgenic plants obtained in the study are proposed as model objects for investigating the role of glucanases in plants.</p>","PeriodicalId":94019,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Inheritance of altered flower morphology and kanamycin resistance in transgenic Tobacco plants]. [转基因烟草植物花形态改变和卡那霉素抗性的遗传]。
Genetika Pub Date : 2001-06-01
A A Zagorskaia, E V Deĭneko, Iu V Sidorchuk, V K Shumnyĭ
{"title":"[Inheritance of altered flower morphology and kanamycin resistance in transgenic Tobacco plants].","authors":"A A Zagorskaia, E V Deĭneko, Iu V Sidorchuk, V K Shumnyĭ","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Inheritance of altered flower morphology and resistance to antibiotic kanamycin was studied in the first and second generations (T1 and T2, respectively) of self-pollinated transgenic tobacco plants. In most transformants, kanamycin resistance was closely linked to mutant phenotype. T-DNA-induced mutations were shown to be dominant.</p>","PeriodicalId":94019,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Production of a SCAR marker in pea Pisum sativum L. using RAPD analysis]. [利用 RAPD 分析在豌豆 Pisum sativum L. 中制作 SCAR 标记]。
Genetika Pub Date : 2001-04-01
O V Koveza, Z G Kokaeva, S A Gostimskiĭ, T V Petrova, E S Osipova
{"title":"[Production of a SCAR marker in pea Pisum sativum L. using RAPD analysis].","authors":"O V Koveza, Z G Kokaeva, S A Gostimskiĭ, T V Petrova, E S Osipova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A polymorphic 750-bp fragment, RAPD marker, specific to particular pea genotypes (line L-111 and the Nord cultivar) was identified. Using this RAPD marker, SCAR was obtained. SCAR inheritance in the first and second generations was studied and its dominant character was shown.</p>","PeriodicalId":94019,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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