[转基因烟草植物表达细菌基因编码的恒温葡聚糖酶]。

Genetika Pub Date : 2001-06-01
N R Movsesian, X Alizade, K A Musiĭchuk, Iu G Popov, E S Piruzian
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引用次数: 0

摘要

研究表明,细菌表达的恒温β-葡聚糖酶基因保留了其活性和底物特异性。胡萝卜扩展蛋白的领导肽能有效地将细菌酶分泌到植物组织的细胞间隙中。在植物组织中表达细菌的 beta-1,3-葡聚糖酶基因会改变其形态发生潜能。与对照品系和表达β-1,3-1,4-葡聚糖酶的转基因品系相比,从这些植物品系的胼胝体中再生出嫩芽需要细胞分裂素(6-BAP)浓度增加 6 到 8 倍。表达β-1,3-葡聚糖酶细菌基因的转基因植株生根速度更快。本研究获得的转基因植物可作为研究葡聚糖酶在植物中作用的模型对象。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Transgenic Tobacco plants expressing bacterial genes encoded the thermostable glucanases].

It is shown that bacterial genes for thermostable beta-glucanases are expressed retaining their activity and substrate specificity. The leader peptide of the carrot extensin exerts effective secretion of the bacterial enzymes into the intercellular space of the plant tissue. Expression of the bacterial gene for beta-1,3-glucanase in plant tissues alters their morphogenetic potential. Regeneration of shoots from the calli of these plant lines requires a six- to eightfold increase in cytokinin (6-BAP) concentration in comparison with the control lines and the transgenic lines expressing beta-1,3-1,4-glucanase. Rooting of transgenic plants expressing the bacterial gene for beta-1,3-glucanase occurs much faster. The transgenic plants obtained in the study are proposed as model objects for investigating the role of glucanases in plants.

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