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引用次数: 0
摘要
大豆基因型认证方法已经开发出来。该程序采用了三种基于聚合酶链式反应（PCR）的 DNA 分析方法：任意引物 PCR（AP PCR）、简单序列重复多态性（SSRP）分析和简单序列间重复（ISSR）分析。建议的认证方法既可用于遗传和育种研究，也可用于种子生产。建议采用一种 "证书 "形式，以反映所研究的每个栽培品种的独特特征。对来自不同生态地理区域的大豆基因型的等位基因分布进行分子遗传分析的结果，允许对个别等位基因的适应意义进行估计。
[Molecular-genetic identification and certification of soybean (Glycine max L.) cultivars].
An approach to certification of soybean genotypes has been developed. The procedure employs three methods of DNA analysis based on polymerase chain reaction (PCR): PCR with arbitrary primers (AP PCR), simple sequence repeat polymorphism (SSRP) analysis, and inter-simple sequence repeat (ISSR) analysis. The approach to certification proposed may be used in both genetic and breeding research and seed production. A "certificate" form that reflects the unique characteristics of each cultivar studied is proposed. The results of molecular genetic analysis of allele distribution in genotypes of soybean from different ecological geographic zones permit estimation of the adaptive significance of individual alleles.
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