European journal of microbiology & immunology最新文献_第7页

Global transcriptomic analysis of breast cancer and normal mammary epithelial cells infected with Borrelia burgdorferi. 伯氏疏螺旋体感染的癌症和正常乳腺上皮细胞的全转录组学分析。

European journal of microbiology & immunology Pub Date : 2023-10-19 Print Date: 2023-11-23 DOI: 10.1556/1886.2023.00031

Vishwa A Khatri, Sambuddha Paul, Niraj Jatin Patel, Sahaja Thippani, Janhavi Y Sawant, Katie L Durkee, Cassandra L Murphy, Geneve Ortiz Aleman, Justine A Valentino, Jasmine Jathan, Anthony Melillo, Eva Sapi

{"title":"Global transcriptomic analysis of breast cancer and normal mammary epithelial cells infected with Borrelia burgdorferi.","authors":"Vishwa A Khatri, Sambuddha Paul, Niraj Jatin Patel, Sahaja Thippani, Janhavi Y Sawant, Katie L Durkee, Cassandra L Murphy, Geneve Ortiz Aleman, Justine A Valentino, Jasmine Jathan, Anthony Melillo, Eva Sapi","doi":"10.1556/1886.2023.00031","DOIUrl":"10.1556/1886.2023.00031","url":null,"abstract":"The bacterial spirochete Borrelia burgdorferi, the causative agent of Lyme Disease, can disseminate and colonize various tissues and organs, orchestrating severe clinical symptoms including arthritis, carditis, and neuroborreliosis. Previous research has demonstrated that breast cancer tissues could provide an ideal habitat for diverse populations of bacteria, including B. burgdorferi, which is associated with a poor prognosis. Recently, we demonstrated that infection with B. burgdorferi enhances the invasion and migration of triple-negative MDA-MB-231 cells which represent a type of breast tumor with more aggressive cancer traits. In this study, we hypothesized that infection by B. burgdorferi affects the expression of cancer-associated genes to effectuate breast cancer phenotypes. We applied the high-throughput technique of RNA-sequencing on B. burgdorferi-infected MDA-MB-231 breast cancer and normal-like MCF10A cells to determine the most differentially expressed genes (DEG) upon infection. Overall, 142 DEGs were identified between uninfected and infected samples in MDA-MB-231 while 95 DEGs were found in MCF10A cells. A major trend of the upregulation of C-X-C and C-C motif chemokine family members as well as genes and pathways was associated with infection, inflammation, and cancer. These genes could serve as potential biomarkers for pathogen-related tumorigenesis and cancer progression which could lead to new therapeutic opportunities.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":" ","pages":"63-76"},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49686566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effect of theaflavin-3,3'-digallate can involve its binding to the "stem" domain of α-hemolysin of Staphylococcus aureus. 茶黄素-3,3'-二没食子酸盐的抑制作用可能涉及其与金黄色葡萄球菌α-溶血素的“茎”结构域的结合。

European journal of microbiology & immunology Pub Date : 2023-10-18 Print Date: 2023-11-23 DOI: 10.1556/1886.2023.00032

Anna Goc, Waldemar Sumera, Matthias Rath, Aleksandra Niedzwiecki

{"title":"Inhibitory effect of theaflavin-3,3'-digallate can involve its binding to the \"stem\" domain of α-hemolysin of Staphylococcus aureus.","authors":"Anna Goc, Waldemar Sumera, Matthias Rath, Aleksandra Niedzwiecki","doi":"10.1556/1886.2023.00032","DOIUrl":"10.1556/1886.2023.00032","url":null,"abstract":"Infections caused by Staphylococcus aureus are currently a worldwide threat affecting millions of individuals. The pathogenicity of S. aureus is associated with numerous virulence factors, including cell surface proteins, polysaccharides, and secreted toxins. The pore-forming α-hemolysin, known as α-toxin, is produced by nearly all virulent strains of S. aureus and is implicated in several diseases including skin and soft tissue infections, atopic dermatitis, and pneumonia. There are currently no vaccines available for the prevention of S. aureus infections and the efficacy of available antibiotics has been fading. In this study we examined the mode of antihemolytic activity of theaflavin-3,3'-digallate against α-hemolysin of methicillin-resistant S. aureus by molecular docking using AutoDock Vina as the molecular docking tool. The theaflavin-3,3'-digallate docked the molecular sequence of the Hla (PDB ID:7ahl). The scores of the top 10 binding modes obtained were between -9.0 and -8.5 kcal mol-1, and the best binding mode was -9.0 kcal mol-1. Direct binding sites of theaflavin-3,3'-digallate to the \"stem\" domain of Hla were revealed which primarily targeted of the residues Met113, Thr117, Asn139. The disclosure of this potential binding mode warrants further clinical evaluation of theaflavin-3,3'-digallate as an anti-hemolytic compound in order to practically validate our results.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":" ","pages":"83-87"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49686567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Very low prevalence of Mansonella perstans-specific cell-free DNA in serum samples of Ghanaian HIV patients. 加纳HIV患者血清样本中的Mansonella perstans特异性无细胞DNA的患病率非常低。

European journal of microbiology & immunology Pub Date : 2023-09-26 DOI: 10.1556/1886.2023.00028

Kirsten Alexandra Eberhardt, Luzia Veletzky, Felix Weinreich, Richard Odame Phillips, Fred Stephen Sarfo, Torsten Feldt, Albert Dompreh, Shadrack Osei Asibey, Richard Boateng, Hagen Frickmann

{"title":"Very low prevalence of Mansonella perstans-specific cell-free DNA in serum samples of Ghanaian HIV patients.","authors":"Kirsten Alexandra Eberhardt, Luzia Veletzky, Felix Weinreich, Richard Odame Phillips, Fred Stephen Sarfo, Torsten Feldt, Albert Dompreh, Shadrack Osei Asibey, Richard Boateng, Hagen Frickmann","doi":"10.1556/1886.2023.00028","DOIUrl":"10.1556/1886.2023.00028","url":null,"abstract":"Background: Mansonellosis is a widely neglected helminth disease which is predominantly observed in tropical regions. This study was conducted to assess potential associations of the prevalence of circulating Mansonella perstans-specific cell-free DNA in human serum and HIV infection in Ghanaian individuals.Methods: For this purpose, serum samples obtained from Ghanaian HIV-patients (n = 989) and non-HIV-infected Ghanaian control individuals (n = 91) were subjected to real-time PCR targeting the ITS-(internal transcribed spacer-)2 sequence of M. perstans and Mansonella sp. Deux.Results: Mansonella-specific cell-free DNA was detected in serum samples of only 2 HIV-positive and 0 HIV-negative individuals, making any reliable conclusions on potential associations between HIV and mansonellosis in tropical Ghana unfeasible.Conclusions: Future epidemiological studies on hypothetical associations between mansonellosis and HIV infections should focus more specifically on high-endemicity settings for both Mansonella spp.-infections and HIV-infections, include higher case numbers and be based on real-time PCR from whole blood rather than from serum, in which only circulating parasite DNA but no more cell-bound parasite DNA can be detected. However, the study did not show associations of HIV infections in Ghanaian individuals with Mansonella worm loads high enough to detect cell-free Mansonella DNA in serum by PCR.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/21/59/eujmi-13-057.PMC10578140.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41171988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and optimization of an improved qPCR assay for the detection of Histoplasma capsulatum. 用于检测荚膜组织浆的改良qPCR检测方法的设计和优化。

European journal of microbiology & immunology Pub Date : 2023-09-21 DOI: 10.1556/1886.2023.00026

Bianca Kelly Neves Izidro da Silva, Ana Cláudia Alves Cortez, Luciana Aires Oliveira, Juan Diego Ribeiro de Almeida, Érica Simplício de Souza, Katia Santana Cruz, Ani Beatriz Jackisch Matsuura, Mauricio Morishi Ogusku, Karolina Jeaneth Solórzano Chavarría, Djane Baía-da-Silva, Quique Bassat, Marcus Vinícius Guimarães Lacerda, Hagen Frickmann, João Vicente Braga de Souza

{"title":"Design and optimization of an improved qPCR assay for the detection of Histoplasma capsulatum.","authors":"Bianca Kelly Neves Izidro da Silva, Ana Cláudia Alves Cortez, Luciana Aires Oliveira, Juan Diego Ribeiro de Almeida, Érica Simplício de Souza, Katia Santana Cruz, Ani Beatriz Jackisch Matsuura, Mauricio Morishi Ogusku, Karolina Jeaneth Solórzano Chavarría, Djane Baía-da-Silva, Quique Bassat, Marcus Vinícius Guimarães Lacerda, Hagen Frickmann, João Vicente Braga de Souza","doi":"10.1556/1886.2023.00026","DOIUrl":"10.1556/1886.2023.00026","url":null,"abstract":"Background: This study aimed at improving a real-time polymerase-chain-reaction (qPCR) assay for the detection of Histoplasma capsulatum, a fungal pathogen that can cause severe respiratory infections in humans, in clinical and soil samples.Methods: Primer and probes were in-silico designed, in-silico and in-vitro evaluated including clinical biopsy materials and finally subjected to a real-world application with collected soil samples.Results: Applying the qPCR assay with liver and lung biopsies from 71 patients each, including 59 patients infected with human immunodeficiency virus (HIV), as well as with Sabouraud (SAB) agar culture as the diagnostic reference standard, diagnostic accuracy of the qPCR assay of 100% (5/5) sensitivity and 96% (63/66) specificity for liver samples and 100% (4/4) sensitivity and 94% (63/67) specificity for the lung samples was recorded. When applying the assay with soil samples from caves near of Presidente Figueiredo city, Amazonas, Brazil, one sample from the Maroaga cave was confirmed as positive.Conclusions: The improved qPCR assessed in this study was successful in detecting H. capsulatum with high efficiency and accuracy in in-vitro evaluation, including the identification of the target pathogen in both clinical and environmental samples.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/58/5e/eujmi-13-037.PMC10578137.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41180057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuberculosis challenges: Resistance, co-infection, diagnosis, and treatment. 结核病挑战：耐药性、合并感染、诊断和治疗。

European journal of microbiology & immunology Pub Date : 2022-04-14 DOI: 10.1556/1886.2021.00021

Mohsen Heidary, Maryam Shirani, Melika Moradi, Mehdi Goudarzi, Ramin Pouriran, Tayebe Rezaeian, Saeed Khoshnood

{"title":"Tuberculosis challenges: Resistance, co-infection, diagnosis, and treatment.","authors":"Mohsen Heidary, Maryam Shirani, Melika Moradi, Mehdi Goudarzi, Ramin Pouriran, Tayebe Rezaeian, Saeed Khoshnood","doi":"10.1556/1886.2021.00021","DOIUrl":"10.1556/1886.2021.00021","url":null,"abstract":"Early diagnosis of tuberculosis (TB), followed by effective treatment, is the cornerstone of global TB control efforts. An estimated 3 million cases of TB remain undetected each year. Early detection and effective management of TB can prevent severe disease and reduce mortality and transmission. Intrinsic and acquired drug resistance of Mycobacterium tuberculosis (MTB) severely restricted the anti-TB therapeutic options, and public health policies are required to preserve the new medications to treat TB. In addition, TB and HIV frequently accelerate the progression of each other, and one disease can enhance the other effect. Overall, TB-HIV co-infections show an adverse bidirectional interaction. For HIV-infected patients, the risk of developing TB disease is approximately 22 times higher than for persons with a protective immune response. Analysis of the current TB challenges is critical to meet the goals of the end TB strategy and can go a long way in eradicating the disease. It provides opportunities for global TB control and demonstrates the efforts required to accelerate eliminating TB. This review will discuss the main challenges of the TB era, including resistance, co-infection, diagnosis, and treatment.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71430403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk Reduction of Needle Stick Injuries Due to Continuous Shift from Unsafe to Safe Instruments at a German University Hospital. 在德国大学医院，由于仪器从不安全到安全的持续转变，针刺伤的风险降低。

European journal of microbiology & immunology Pub Date : 2016-08-23 eCollection Date: 2016-09-29 DOI: 10.1556/1886.2016.00025

Hagen Frickmann, Wibke Schmeja, Emil Reisinger, Thomas Mittlmeier, Karen Mitzner, Norbert Georg Schwarz, Philipp Warnke, Andreas Podbielski

{"title":"Risk Reduction of Needle Stick Injuries Due to Continuous Shift from Unsafe to Safe Instruments at a German University Hospital.","authors":"Hagen Frickmann, Wibke Schmeja, Emil Reisinger, Thomas Mittlmeier, Karen Mitzner, Norbert Georg Schwarz, Philipp Warnke, Andreas Podbielski","doi":"10.1556/1886.2016.00025","DOIUrl":"https://doi.org/10.1556/1886.2016.00025","url":null,"abstract":"This study assessed protective effects of a continuous introduction of safe instruments in terms of reduction of needle stick injuries. The retrospective study analyzed correlations between the increasing proportion of safe instruments and a reduction of the incidence of needle stick injuries linked to such instruments in a German university hospital over 5 years. Incidents declined about 17.6% from 80.3 incidents per 1000 employees to 66.2, associated with an increase in the proportions of injuries due to instruments without protective mechanisms such as scalpels or hypodermic needles by 12.2%. For injuries due to venipuncture cannulae in various surgical and internal medicine departments, there was a negative association between the proportion of safe instruments and the incidence of injuries. For injection needles, portacath needles, and lancets in selected internal medicine departments, the number of injuries also dropped during this study interval. However, there was no clear-cut association with the percentage of safe instruments. This observational study suggests a correlation between the implementation of use of safe instruments and the reduction of needle stick injuries in a case of a graduated implementation. However, the effects are much less pronounced than in previous interventional studies.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":"6 3","pages":"227-237"},"PeriodicalIF":0.0,"publicationDate":"2016-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/1886.2016.00025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72212273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Experimental Models of Ocular Infection with Toxoplasma Gondii. 弓形虫眼部感染的实验模型。

European journal of microbiology & immunology Pub Date : 2015-12-04 eCollection Date: 2015-12-01 DOI: 10.1556/1886.2015.00045

Agata Dukaczewska, Roberto Tedesco, Oliver Liesenfeld

{"title":"Experimental Models of Ocular Infection with Toxoplasma Gondii.","authors":"Agata Dukaczewska, Roberto Tedesco, Oliver Liesenfeld","doi":"10.1556/1886.2015.00045","DOIUrl":"10.1556/1886.2015.00045","url":null,"abstract":"Ocular toxoplasmosis is a vision-threatening disease and the major cause of posterior uveitis worldwide. In spite of the continuing global burden of ocular toxoplasmosis, many critical aspects of disease including the therapeutic approach to ocular toxoplasmosis are still under debate. To assist in addressing many aspects of the disease, numerous experimental models of ocular toxoplasmosis have been established. In this article, we present an overview on in vitro, ex vivo, and in vivo models of ocular toxoplasmosis available to date. Experimental studies on ocular toxoplasmosis have recently focused on mice. However, the majority of murine models established so far are based on intraperitoneal and intraocular infection with Toxoplasma gondii. We therefore also present results obtained in an in vivo model using peroral infection of C57BL/6 and NMRI mice that reflects the natural route of infection and mimics the disease course in humans. While advances have been made in ex vivo model systems or larger animals to investigate specific aspects of ocular toxoplasmosis, laboratory mice continue to be the experimental model of choice for the investigation of ocular toxoplasmosis. ","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":"5 4","pages":"293-305"},"PeriodicalIF":0.0,"publicationDate":"2015-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4681357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71435047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0