Buffalo Bulletin最新文献

{"title":"Development of ELISA kit for serological diagnosis of Q fever","authors":"T. Gülhan, M. Sezener, Şeyda Yaman, A. Fi̇ndi̇k, A. Çiftçi","doi":"10.56825/bufbu.2023.4224146","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4224146","url":null,"abstract":"In this study, it was aimed to develop an ELISA diagnostic kit that can be used in the serological investigation of Q fever disease caused by C. burnetii. For this purpose, bovine, sheep, goat and buffalo sera were tested with a commercial ELISA kit and home-made ELISA kit developed using two different conjugates. Of the 92 buffaloes blood serum samples tested, 22 (23.9%) were found positive by commercial ELISA kit, 7 (7.6%) and 29 (31.5%) were positive by protein-A conjugated ELISA and 29 anti-bovine IgG conjugated ELISA, respectively. In addition, Q fever positive and negative cattle, sheep, and goat blood sera that were found to be positive and negative for Q fever in a previous study conducted on animals with abortion history were also re-examined in our laboratory. In conclusion, within the scope of the project, an alternative homemade ELISA kit was developed for the serological diagnosis of Q fever and serum samples of the target species of buffalo, cattle, sheep, and goats were tested. Thus, the home-made ELISA kit, which can be used in research aimed at detecting seropositivity, especially in buffalo sera, was made ready for use. It was concluded that the data obtained would contribute to wider ranging epidemiological studies. The epidemiology of the disease can be revealed in detail in the light of research to be carried out with more comprehensive projects in our region.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47524605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transpalpebral exenteration of eye for recurrent squamous cell carcinoma in a Nili-Ravi buffalo – A case report","authors":"Urfeya Mirza, Sukhnandan Singh, U. Farooq","doi":"10.56825/bufbu.2023.4224783","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4224783","url":null,"abstract":"In this article, the treatment of squamous cell carcinoma surgically in an eye of an 8-year-old Nili-Ravi buffalo with entire vision loss is described. The positive result of this case indicates that radical exenteration of the orbit is a feasible option for alleviating the distress of bovines with squamous cell carcinoma of the eyes.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46388505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of melatonin implantation prior to ovsynch plus cidr protocol on subsequent ovarian activity and pregnancy rate in summer anestrous Murrah buffaloes","authors":"S. Ghuman, M. Honparkhe, Bilawal Singh","doi":"10.56825/bufbu.2023.4223586","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4223586","url":null,"abstract":"For assessing the impact of melatonin pre-treatment in summer anestrous Murrah buffaloes on subsequent reproductive activity of a fixed time artificial insemination (FTAI) protocol, 83 anestrous Murrah buffaloes were subcutaneously inserted 2x4 mm size absorbable melatonin (MT) implants (18 mg/50 kg b wt) at the base of left ear and 38 Murrah buffaloes were used as non-implanted controls. On day 35 post-MT, the treatment and control group Murrah buffaloes were subjected to FTAI protocol (Ovsynch plus CIDR). Ovarian ultrasonography and jugular vein blood sampling was carried out on day 7 and 10 of protocol and on day 7-post AI. Overall pregnancy rate on day 90 post-AI in MT group was higher compared to control group (55.4 vs 31.6%, P<0.05), along with the higher (P>0.05) plasma progesterone on day 7 of FTAI protocol in Murrah buffaloes of the former group which conceived subsequently compared to their non-pregnant counterparts. Moreover, MT group had higher (P>0.05) increase in dominant follicle diameter between day 7 and 10 of protocol compared to control group. On day 7 post-AI, the luteal profile in pregnant murrah buffaloes of MT group was better (P<0.05) compared to their counterparts in control group (CL: 14.80±0.70 vs 10.82±0.46 mm, P4: 1.54±0.07 vs 1.04±0.08 ng/ml). In conclusion, melatonin pre-treatment in summer anestrous Murrah buffaloes could be associated with better luteal activity and higher pregnancy rate in the Ovsynch+CIDR protocol.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48843166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence and haematobiochemical study of amphistomosis in buffaloes (Bubalus bubalis) in Mhow, Madhya Pradesh","authors":"Rahul Khurana, G. Jatav, A. Jayraw, S. Shukla, Nidhi Krishna Shrivastava, V. Agrawal, M. Shakya","doi":"10.56825/bufbu.2023.4224514","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4224514","url":null,"abstract":"In the present study, samples from 100 buffaloes of either sex were collected from slaughterhouse located at Cantonment Board Mhow for the detection of ruminal amphistomosis. Blood samples with or without anticoagulant were collected to study the haematobiochemical changes in buffaloes suffered from amphistomosis. Amphistomes were also collected for morphological identification of amphistome species in slaughtered animals. The prevalence of ruminal amphistomes was found to be 8%. The infected buffaloes in this study showed a reduction in the mean values of Hb, TEC and PCV. There was an increase in TLC and neutrophil count. Lymphocytes showed a slight decrease in their mean values and no significant changes were seen in values of basophil and eosinophil count. The infected buffaloes in this study showed a reduction in the mean values of total protein. The mean values of SGOT, SGPT and alkaline phosphatase showed an increment in the infected buffalo.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44001848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of photoperiod and other environmental factors on the oocyte populations of water buffalo (Bubalus bubalis) in Veracruz state, Mexico","authors":"Ivan Avalos-Rosario","doi":"10.56825/bufbu.2023.4225352","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4225352","url":null,"abstract":"The objective of this study was to investigate the effect of photoperiod and other environmental factors on the population of Water buffalo oocytes in Veracruz, Mexico. Oocytes (n=123) were obtained by follicular aspiration and graded according to morphological characteristics. Data regarding regional environmental factors (temperature, humidity, and daylight) were collected from a historic database of a metrological station. Statistical analysis was performed through Person correlation, one-way ANOVA and linear regression analysis, P<0.05 was considered significant. There was a correlation (P<0.05) between oocyte populations (total and viable) with temperature, humidity, and duration of daylight. ITH (index of temperature and humidity) and oocyte viability did not correlate (P>0.05) with other variables. Oocyte populations (total and viable) and environmental variables changed (P<0.05) during all the sampled months. Oocyte populations (total and viable) were affected (P<0.05) by temperature (r=-0.63; r=-0.57), humidity (r=0.46; r=0.39) and daylight (r=-0.86; r=-0.87), no association (P>0.05) with ITH or oocyte viability were identified. In conclusion, temperature, humidity, and daylight affected the oocyte populations (total and viable). Photoperiod seemed to be the most important factor affecting oocyte populations of water buffalos in Veracruz state, Mexico.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41310482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of buparvaquone in pcr confirmed concurrent infection of Trypansoma evansi and Theileria annulata in buffaloes","authors":"V. Agrawal, G. Das, Amit Jaiswal, A. Jayraw, G. Jatav, M. Shakya, G. Goyal","doi":"10.56825/bufbu.2023.4224141","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4224141","url":null,"abstract":"Five buffaloes, 5 to 7 years old in the month of July 2015 with history of high rise of temperature, nasal discharge, exophthalmia, excitement, lacrimation, salivation, dyspnea, generalised lymphadenopathy, especially in the prescapular lymph nodes were attended at a farm in Mahakaushal region of Madhypradesh. By blood smear and polymerase chain reaction analysis, it was determined that all the buffaloes had mixed infections of T. evansi and T. annulata. The treatment of all five buffaloes was initiated with single deep intramuscular injection of diminazine acceturate 3.5 mg/kg body weight along with three dosages of long acting oxytetracycline 20 mg/kg b.wt. on alternate day. A single injection of buparvaquone on the fourth day (in 3 cases) and two doses of buparvaquone (2.5 mg kg-1) at 48 h intervals (in 2 cases) were administered. Only 3 buffaloes showed uneventful recovery after a single injection with buparvaquone. When the blood samples of all the five buffaloes were again analyzed by PCR and found all the samples were negative for T. evansi and T. annulata except two buffaloes (Two cases hading two doses of buparvaquone) which were positive for T. annulata in spite of giving the second dose of  buparvaquone. The study indicated that there might be reduced efficacy or resistance of T. annulata against buparvaquone.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45517093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological studies on spontaneous urinary bladder lesions in Indian buffaloes","authors":"B. Jangir, R. Somvanshi","doi":"10.56825/bufbu.2023.4223585","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4223585","url":null,"abstract":"The present investigation describes the spontaneous pathological lesions in urinary bladders (n = 177) of slaughtered buffaloes collected from Northern India. Gross examination revealed congestion, haemorrhages, oedematous changes and thickening of mucosa. Microscopically, epithelial abnormalities, inflammatory and neoplastic conditions were noticed. Epithelial abnormalities included epithelial hyperplasia, von Brunn’s nests, cystitis cystica and cystitis glandularis. Inflammatory conditions such as congestion, haemorrhages and cystitis were noticed. Cystitis was classified as lymphocytic, plasmo-lymphocytic, follicular and interstitial types. However, only single case of haemangiosarcoma was observed. Polymerase chain reaction revealed the presence of DNA of only BPV-1 in one sample out of 21, while one sample showed the presence of DNA of both BPV-1 and BPV-2. On immunohistochemistry, Ki-67 expression was observed only in haemangiosarcoma and one case of plasmo-lymphocytic cystitis with immunostaining restricted to nuclei. COX-2 immunostaining was observed in one case each of flat hyperplasia, von Brunn’s nest, haemangiosarcoma and two cases of plasmo-lymphocytic cystitis. Among epithelial abnormalities, COX-2 immunostaining was restricted to epithelial component of the lesions, and it was diffused cytoplasmic type with occasional perinuclear staining particularly in the cytoplasmic membrane. However, in haemangiosarcoma the COX-2 immunoreactivity was noticed in epithelial as well as connective tissue stroma. In conclusion, it may be stated that urinary bladders of slaughtered buffaloes showed a number of epithelial abnormalities, inflammatory conditions and occasionally neoplasms. Further studies to explore the infectious as well as non-infectious etiologies should be undertaken.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43910649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of body condition and occurrence of anoestrus in buffaloes","authors":"Santosh Kumar Daheriya, Madhu Shivhare, S. P. Shukla, S. Nema","doi":"10.56825/bufbu.2023.4224725","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4224725","url":null,"abstract":"The present study was conducted on 30 buffaloes belonging to department of veterinary gynaecology and obstetrics and village around college of Veterinary Science and Animal Husbandry, Mhow (M.P.), buffalo were selected on the basis of history, which had not exhibited oestrus for 90 days or more postpartum, clinical examination of these buffaloes were made for general health and reproductive soundness, per rectally explored twice 10 days apart to confirm ovarian activity and genital status, the animals is awarded an appropriate body condition score 1 (very thin) to 5 (fat) on practice  half score were used for animals whose condition score is intermediate. the experiment was done on 30 buffaloes divided into 6 groups, each group consist of 6 buffaloes. The body condition score on scale 1 (very thin) to 5 (fat) ranged between. Body condition score in anoestrus buffaloes was significantly lower than the normal cycling buffaloes. There is a positive relationship between body condition score and subsequent reproductive performance of the animals.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48389680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical findings, haemato-biochemical alterations and conservative treatment of caecal dilatation in a buffalo","authors":"C. Bhutia, S. Uppal, S. A. Hussain","doi":"10.56825/bufbu.2023.4222615","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4222615","url":null,"abstract":"This article describes the clinical findings, haemato-biochemical alterations, and conservative treatment of a buffalo suffering from caecal dilatation. The clinical findings were anorexia, scanty faces, reduced rumen motility, distension of right flank, ping sounds on auscultation and percussion of right flank and dilated caecum at pelvic brim on per-rectal examination. The hemato-biochemical alterations were neutrophilic leukocytosis, increased AST, BUN and lactate, and low calcium levels. Conservative treatment resulted in uneventfully after a period of five days.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47479414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrus and ovulation synchrony of buffaloes (Bubalus bubalis): A review","authors":"G. R. Bhat, Gurucharan Singh Dhaliwal","doi":"10.56825/bufbu.2023.4222415","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4222415","url":null,"abstract":"Synchronizing estrus and ovulation in buffalo using timed inseminations (TAI) protocols circumvents the limitations associated with estrus detection. Spontaneous estrus in buffalo may achieve more conception rate on insemination than those subjected to synchronization of estrus and timed insemination (TAI). The advantages of insemination following synchronization programs include higher detection rates combined with decreased embryonic losses and reduction in days open. A synchonization programme should ensure control of estrus cycle which entirely depends on ovarian status of animals before starting any protocol. Ovarian follicular status at the beginning of treatment protocols plays vital role for effective response shown by animals. Studies show that more precise alterations in follicular waves may be needed to achieve better synchrony of ovulation and more fertility. The protocols which use Gonadotropic Releasing Hormone (GnRH), follicle stimulating hormone (FSH), luteinizing hormone (LH), equine chorionic gonadotropin (eCG), human chorionic gonadotropin (hCG), prostaglandins, progesterone and estradiol have been helpful in achieving the estrus and ovulation synchrony. Use of estradiol esters and progesterone per-vaginum implants have also been incorporated in synchronization programs. Synchronous emergence of follicular waves using trans-vaginal ultrasound guided follicular ablation and estradiol administration is a recent concept for synchronization in buffalo. Further, a recent approach has been to allow the follicle of second follicular wave to ovulate as the fertility has been lesser when first wave follicle ovulates. Moreover, controlling environmental influences, nutritional and managemental strategies remain pre-requisite to achieve desired goals though hormonal administration. This is more particular in buffaloes suffering from anestrum more so in low or non-breeding season and pre-pubetal anestrum. This review will cover the available documented literature regarding estrous cycle physiology with emphasis on follicular wave dynamics and the future area of work to be focused to achieve more reproductive efficiency in buffalo.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42770735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}