{"title":"Assessing the acceptability of hormone-based estrus induction / synchronization protocols to overcome anestrus in buffaloes","authors":"Mallikerimath Mahantswamy, Sarvpreet Singh Ghuman, Bilawal Singh","doi":"10.56825/bufbu.2023.4233575","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4233575","url":null,"abstract":"Five hundred dairy households in the rural areas of Punjab state were interviewed through comprehensively designed questionnaires to assess the knowledge and experience of farmers regarding the hormone-based strategies to overcome anestrus and getting the buffaloes pregnant. About 81.8% dairy households were unaware (P<0.05) about any estrus induction or synchronization hormonal protocol and majority (65.5%) of these belonged to households with <5 animals. Out of households having awareness about hormonal protocol use in buffaloes, the majority (58.2%) belonged to households with >15 animal. Out of dairy farmers using hormonal protocols, the proportion of illiterate was 14.8%. Out of dairy farmers not aware about hormonal protocols, the proportion of illiterate was 52.3%. Data analysis of 91 dairy households who had used hormonal protocols revealed summer (65.9%) and anestrus (82%) as the major underlying reasons for their use in buffaloes. Low success rate (20.2%), high cost (50%) and non-availability of doctor (29.7%) were the reasons for the irregular use of hormonal protocols. The success rate of hormonal protocols under field condition in terms of conception was 47.1%. In summary, the major bottlenecks in acceptability of hormone-based therapeutics strategies under filed conditions are illiteracy, high cost of therapeutics strategies and lack of awareness in buffalo farmers especially in households with <5 animals.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135082845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Use of hyaluronidase enzyme for hastening the cervical dilatation in successfully detorted uterine torsion affected buffaloes","authors":"Randhir Singh, Sarvpreet Singh Ghuman, Navdeep Singh","doi":"10.56825/bufbu.2023.4233570","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4233570","url":null,"abstract":"The successfully detorted uterine torsion buffaloes were either subjected to routine post-detorsion treatment (n=10) or in addition to routine treatment, intracervical hyaluronidase enzyme was administered (10,000 IU; 2.5 ml at each of 3, 6, 9, 12 o’clock position of cervix) immediately post-detorsion (n=10), followed by repeated administration at 6h interval (0, 6, 12, 18 h) till complete cervical dilatation or till 24 h post-detorsion. The time elapsed between successful uterine detorsion to complete cervical dilatation was reduced (P<0.05) in buffaloes subjected to enzyme treatment in comparison to their control counterparts (754.6±114.0 vs. 213.5±33.1 minutes). In buffaloes delivering dead fetus, following intracervical treatment, the interval between detorsion to complete cervical dilatation was reduced (P<0.05) to 240.8±44.0 minutes in comparison to their control counterparts (838.1±126.2 minutes). Moreover, following hyaluronidase treatment, the time interval from detorsion of a buffalo to complete cervical dilatation with respect to degree of uterine torsion was much lower (P<0.05) in treated compared to their control group counterparts having either <180o or ≥180o uterine torsion. The fertility parameters (number of days to first estrus and duration of service period) were improved (P>0.05) in hyaluronidase-treated buffaloes of both the groups (<18 h or ≥18 h duration of torsion) as compared to their control counterparts. Conclusion: Intracervical hyaluronidase treatment hastens the cervical dilatation in successfully detorted uterine torsion affected buffaloes with a subsequent potentially beneficial impact on fertility.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135082982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of some non-genetic factors on productive and reproductive traits of Mehsana buffaloes","authors":"Rishit Navinchandra Sathwara, Jay Prakash Gupta, Jagdish Devji Chaudhari, Pravin Ambabhai Patel","doi":"10.56825/bufbu.2023.4233965","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4233965","url":null,"abstract":"The records of 7782 Mehsana buffaloes sired by 184 sires maintained at Dudhsagar Research and Development Association (DURDA), Dudhsagar Dairy, Mehsana, Gujarat born during 1989 to 2012 were collected and analysed to examine the effect of non-genetic factors viz. period and season of calving and age at first calving. The least squares analysis was used for estimation of various non-genetic factors on First Lactation Milk Yield (FLMY), First Lactation Fat Yield (FLFY), Average Fat Percentage (AFP) and Age at First Calving (AFC). The least squares mean with standard error of FLMY, FLMY, AFP and AFC were estimated to be 1900.68±7.65 lit., 135.04±0.57 kg, 7.11±0.11 percentage and 1407.18±4.36 days, respectively. Highly significant (P≤0.01) effect of all the non-genetic factors (period and season of calving and age at first alving group) under study was observed on FLMY, FLMY and AFP in Mehsana buffaloes. Further, highly significant (P≤0.01) effect of season and period of birth was recorded on AFC. It is worthy to note that in Mehsana breed evaluation programme these factors should be considered as they have great influence of them.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135082835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The impact of intracervical hyaluronidase enzyme on cervical histomorphology in successfully detorted uterine torsion affected buffaloes","authors":"Randhir Singh, Sarvpreet Singh Ghuman, Devendra Pathak, Navdeep Singh","doi":"10.56825/bufbu.2023.4233577","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4233577","url":null,"abstract":"The present study evaluated the impact of intracervical hyaluronidase enzyme on cervical histomorphology in successfully detorted uterine torsion affected buffaloes. These animals were either subjected to routine post-detorsion treatment (n=10) or in addition to routine treatment, hyaluronidase enzyme (10,000 IU; 2.5 ml at each of 3, 6, 9, 12 o’clock position of cervix) was administered intracervically immediately post-detorsion (n=10), followed by repeated administration at 6 h interval (0, 6, 12, 18 h) till complete cervical dilatation or till 24 h after detorsion. The pre-treatment cervical biopsy samples were collected immediately after detorsion and post-treatment samples were collected at complete cervical dilatation which happened within 24 h after detorsion in all the cases. Following histomorphology, the quantification of collagen fibers as mean percentage area occupied collagen out of total tissue area in pre-treatment cervical biopsy samples of all the buffaloes was revealed as 21.0±5.7. During post-treatment period, at the time of complete cervical dilatation, this value exhibited a decrease in control (9.1±2.2; P>0.05) as well as hyaluronidase group (5.8±2.1; P<0.05). In summary, collagen fiber dispersion in cervical tissue of successfully detorted uterine torsion affected buffaloes suggested their role in cervical dilatation, whereby, intracervical hyaluronidase was able to enhance the cervical tissue collagen dispersion.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135082836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buffalo BulletinPub Date : 2023-09-29DOI: 10.56825/bufbu.2023.4233089
Amar Nasir, Arbab Sikandar, Abdul Shakoor, Muhammad Kashif, Rana Waqar Tabish, Asghar Hussain, Adnan Hassan Tahir, Mazhar Abbas, Muhammad Umer Iqbal
{"title":"Detection of subclinical ketosis in dairy buffalo herds of Tehsil Jhang, Punjab, Pakistan","authors":"Amar Nasir, Arbab Sikandar, Abdul Shakoor, Muhammad Kashif, Rana Waqar Tabish, Asghar Hussain, Adnan Hassan Tahir, Mazhar Abbas, Muhammad Umer Iqbal","doi":"10.56825/bufbu.2023.4233089","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4233089","url":null,"abstract":"Bovine ketosis is a major metabolic disorder emerging as a result of negative energy balance in the post calving period and subsequent lactation of lactating dairy animals. The ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone etc.) are accumulated that can be detected in milk, blood, and urine samples. The present study was accomplished to sort out the frequency of bovine ketosis in dairy buffalo herds during the first 2 months of lactation in buffalo to detect bovine ketosis. For this purpose, blood, and urine samples (n=50) were randomly collected from private dairy farms (n=5) having buffalos as a pilot study employing commercial test strips i.e., Free Style Optium β-ketone test strips (Abbot Healthcare, Pvt. Ltd. UK) for blood and JusChek (Muenster, Germany) for urine analysis. Resultantly, as a whole prevalence of bovine ketosis found using blood sample was 18% and via urine was 12% that indicates high prevalence of subclinical ketosis during 2nd month of lactation. Animals in the age group of 5 to 7 years were found to be significantly more susceptible (P<0.05) with an incidence of 37.5%. The results revealed that a significant subclinical ketosis exists in the dairy buffaloes, which causes decreased production associated with significant economic loss to the farmers. It was concluded that a moderately high incidence of sub clinical bovine ketosis predominates in the study area which may be curtailed by early diagnostic and remedial approaches using field tests to save the economics of the farmers.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135296060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buffalo BulletinPub Date : 2023-09-29DOI: 10.56825/bufbu.2023.4233521
Balbir Singh Khadda, Kanak Lata, Raj Kumar
{"title":"Effect of Pashu chocolate supplementation on production and reproductive performance of milch buffalo under field conditions of semi arid region","authors":"Balbir Singh Khadda, Kanak Lata, Raj Kumar","doi":"10.56825/bufbu.2023.4233521","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4233521","url":null,"abstract":"Pashu chocolate is the brand name of the urea molasses mineral block formulated by KVK, Panchmahal by using of urea, molasses, wheat bran, area specific mineral mixture, black salt, etc. for augment milk production and reproductive performance of dairy animals. A study was conducted to find out the efficacy of Pashu chocolate supplementation on productive and reproductive performance of milking buffalo and feed economics at farmers field in semi-arid zone, during the year 2018 to 2019. Twenty, lactating Mehsana breed of buffalo were randomly selected and distributed equally in to two groups ten each in a completely randomized design i.e., T1 (Control) and T2 (Pashu chocolate supplementations). In the feeding schedule of the present study T1 animals were given 5 to 6 kg dry roughage (sorghum/pearl millet) with green fodder (20 kg Napier-lucerne) added to 2.5 kg concentrate feed/ day, whereas an ad lib lick of UMMB (Pashu chocolate) was offered in the T2 Group in addition to the feeding schedule of T1 Group. An increase of 24.56% in milk i.e., from 6.23±0.40 to 7.76±0.54 L per day was recorded after comparing the outcomes of the two treatments. This significant (P˂0.05) increase in milk production indicates a positive impact of Pashu chocolate on the performance of milch buffaloes. Supplementation of Pashu chocolate as an ad lib lick reduced the postpartum estrus period and service period from 79.7±7.43 and 132.00±13.34 days to 54.3±4.41 and 81.60±7.54 days, respectively. Hence, the findings in the present study indicate beneficial effects of Pashu chocolate on productive and reproductive traits of buffaloes in semi-arid climatic conditions.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135295919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buffalo BulletinPub Date : 2023-09-29DOI: 10.56825/bufbu.2023.4232682
Nunna Veera Venkata Hari Krishna
{"title":"Dystocia due to external hydrocephalus in a primiparous buffalo heifer","authors":"Nunna Veera Venkata Hari Krishna","doi":"10.56825/bufbu.2023.4232682","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4232682","url":null,"abstract":"A primiparous non-descript buffalo heifer in ninth month of gestation was presented for showing the signs of abortion, i.e., vaginal discharges and rupture of water bag (choreo-allantois). Obstetrical examination revealed an open cervix. The foetus was in anterior longitudinal presentation, dorso-sacral position and was locked in pelvic cavity. A round sructure was observed in the pelvic cavity. Based on history and clinical observations, it was diagnosed as late gestational threatened abortion. It was decided to deliver the calf by manual traction, if not possible, by cesarean section. Manual traction on the foetus using snares was applied and a dead foetus with hydrocephalus was delivered per vagina.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135295925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buffalo BulletinPub Date : 2023-06-30DOI: 10.56825/bufbu.2023.4225257
H. Guler, G. Yarim, M. Yarım, O. Duru, Ayris Gokceoglu
{"title":"The milk IGF-2 level is positively correlated with milk yield in Anatolian water buffaloes","authors":"H. Guler, G. Yarim, M. Yarım, O. Duru, Ayris Gokceoglu","doi":"10.56825/bufbu.2023.4225257","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4225257","url":null,"abstract":"The aim of this study was to investigate the relationship between milk insulin-like growth factor-2 (IGF-2) concentration and milk yield in Anatolian water buffaloes. This study was conducted on milk samples from 80 Anatolian water buffaloes. Milk samples collected from buffaloes were evaluated for subclinical mastitis. For this purpose, the California mastitis test and somatic cell count were performed in milk samples taken from four different mammary lobes of buffaloes. The milk IGF-2 level was determined by an enzyme-linked immunosorbent assay kit. Milk IGF-2 concentration ranged from 21.6 ng/ml - 63.2 ng/ml in Anatolian water buffaloes. The IGF-2 concentration in Anatolian water buffaloes milk was 40.1±8.5 ng/ml. Milk IGF-2 was positively correlated with milk yield (r2=0.941, P<0.01). Our results showed that milk IGF-2 concentration was associated with milk yield in Anatolian buffaloes. These findings show that locally synthesized IGF-2 can affect milk yield. This study contributes to the understanding of the composition of buffalo milk, which has an important value in human nutrition. It is recommended to confirm the results of similar measurements in milk from other animal species used for human consumption.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49292810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Modified vulval truss technique to prevent recurrence of cervico-vaginal prolapse in water buffaloes","authors":"Maniprabhu Selvaraju, Subramanian Prakash, V. Varudharajan, Mani Periyannan, Kaliannan Ravikumar, Mahakrishnan . Palanisamy","doi":"10.56825/bufbu.2023.4223888","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4223888","url":null,"abstract":"A retrospective study was conducted in a total of 89 water buffaloes reported with cervico-vaginal prolapse (CVP) in various degrees and were initially examined for their physiological and haematological parameters. The affected buffaloes were classified into 3 groups based on the degree of CVP and were treated with 8 'R' principles and as a recurrence prevention strategy, they were managed with modified conventional vulval truss method, vulval retention suture method and both in all the three groups. The buffaloes handled with modified conventional vulval truss method showed reduced straining, genital injury and no obstetrical complications. The buffaloes treated with vulval retention suture showed mild straining, wound around the sutured site and those buffaloes treated with both modified conventional vulval truss method and vulval retention suture showed relatively effective results. Based on this study, it is concluded that the buffaloes affected with any degree of CVP and treated with modified conventional vulval truss method showed effective recurrence prevention with relatively no swelling, straining, haemorrhage, injury, maggot wound and postpartum obstetrical complications.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44606945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buffalo BulletinPub Date : 2023-06-30DOI: 10.56825/bufbu.2023.4225055
S. A. Shergojry, A. Verma, I. D. Gupta, N. A. Mir
{"title":"Single nucleotide polymorphism, haplotypes and genotypes of Mannose-binding lectin (MBL1) gene and their association with clinical mastitis in Murrah buffalo","authors":"S. A. Shergojry, A. Verma, I. D. Gupta, N. A. Mir","doi":"10.56825/bufbu.2023.4225055","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4225055","url":null,"abstract":"The Mannose-binding lectin (MBL1) gene is an important component of immune response system. It plays vital role in activation of the complement system and act as chief defense molecule of host cell to provide protection against various diseases. In the recent study, six novel single nucleotide polymorphisms (SNPs), at 2689G>C, 2751A>G, 4822T>C, 4853A>G, 4855T>C, and 4978T>C resulted in 2 non synonymous type of change at 4853A>G, 4855T>C resulting in one amino acid substitution Ser150Gly of MBL1 protein and their association with clinical mastitis were investigated in two hundred (200) Murrah buffaloes. These SNPs in MBL1 were genotyped by using the polymerase chain reaction (PCR) and Deoxyribose nucleic acid (DNA) sequencing methods in order to reveal the association with clinical mastitis. SHEsis software tool was used for construction of haplotypes and Linkage disequilibrium analysis. Twenty one (21) haplotypes were constructed. Allelic association analysis showed that C allele of 2689 G>C, A allele of 2751 A>G, C allele of 4822 T>C, A allele of 4853 A>G, C allele of 4855 T>C, and C allele of 4978 T>C had significant association with increased risk of clinical mastitis in Murrah buffaloes (P<0.05). Murrah buffaloes with CG genotype of 2689 G>C, AG or GG genotype of 2751 A>G, CC genotype of 4822T>C, AG or GG genotype of 4853 A>G, TC or CC genotype of 4855 T>C and CC genotype of 4978 T>C loci had significantly lower incidence of clinical mastitis compared to their counter genotypes. Haplotype association analysis showed that Hap21 (TATTGA) was found significantly related to higher risk of clinical mastitis (P<0.05). Hap14 (TGTTGG) and Hap5 (CGCCCA) were found significantly associated with lower risk of clinical mastitis in Murrah buffalo (P<0.05).","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49593993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}