Bone最新文献

{"title":"Altered tissue-to-fibril strain transfer in cortical bone from juvenile female Klotho-deficient mice.","authors":"Kaiyuan Xue, Fanshuo Wang, Li Xi, Himadri Gupta, Nick Terrill, Ying Li","doi":"10.1016/j.bone.2026.118133","DOIUrl":"10.1016/j.bone.2026.118133","url":null,"abstract":"<p><p>Juvenile female Klotho-deficient mice have severe cortical abnormalities, but how the remaining bone matrix partitions deformation across length scales is unclear. We combined in situ tensile testing with synchrotron small-angle X-ray diffraction to quantify tissue and mineralized-collagen-fibril deformation in femoral cortical bone from 5-week-old female wild-type, heterozygous, and homozygous Klotho-deficient mice (n = 5 per group). Quantitative backscattered electron imaging (qBEI) assessed tibial cortical thickness, mineralization and the dark-area fraction of the same sections; phase-contrast microtomography of femur provided qualitative structural context. Effective fibril modulus was higher in heterozygous (14.6 ± 3.5 GPa, p = 0.012) and homozygous mice (13.4 ± 5.5 GPa, p = 0.010) than in wild-type mice (4.3 ± 0.8 GPa). The fibril-to-tissue strain ratio was lower in both Klotho-deficient groups (p = 0.018 and p = 0.028), whereas fibril-orientation dispersion did not differ (p = 0.823). Tissue modulus, failure strain, and failure stress showed no statistically significant genotype differences. Tibial cortices were thinner in both Klotho-deficient groups; the dark-area fraction was higher in homozygous mice (p < 0.001), and mean calcium content was lower in homozygous than wild-type mice (p = 0.011). Mineralization heterogeneity did not differ (p = 0.811). These exploratory findings are consistent with altered tissue-to-fibril strain partitioning associated with Klotho deficiency, together with marked cortical structural deficits, but do not establish the molecular origin of the response. The local X-ray dose was estimated at approximately 0.6 kGy per measurement location, well below the absorbed dose at which X-ray irradiation alters the deformation behaviour of cortical bone. Only female mice at a single juvenile age were studied, so the findings cannot be extrapolated to males or to older animals.</p>","PeriodicalId":93913,"journal":{"name":"Bone","volume":" ","pages":"118133"},"PeriodicalIF":3.6,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149198304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient-specific zebrafish models reveal a complex phenotypic spectrum in NBAS-associated atypical osteogenesis imperfecta.","authors":"Daniel A Baird, Sookyung Seo, Zuzanna Matelowska, Karthick Navin Mohandass, Alagumeenal Subramanian Annamalai, Aynour Abouelkhair, Myar Zafar, Nurhaziqah Supari, Sarah Baxendale, Catherine A Loynes, Freek van Eeden, Meena Balasubramanian","doi":"10.1016/j.bone.2026.118129","DOIUrl":"https://doi.org/10.1016/j.bone.2026.118129","url":null,"abstract":"<p><p>Neuroblastoma amplified sequence gene (NBAS) variants are associated with short stature, optic atrophy, and Pelger-Huët anomaly (SOPH) syndrome. We previously identified compound heterozygous variants in NBAS to cause atypical Osteogenesis Imperfecta (OI), with these patients presenting with short stature, developmental delay, and recurrent long-bone fractures. However, skeletal disease progression due to these variants and the disease mechanisms underlying NBAS-associated OI remain poorly understood. Here, we provide a clinical update on previously identified patients and investigate the role of NBAS during skeletal development using zebrafish knockout and patient-specific missense variant zebrafish models. Homozygous knockout larvae exhibited delayed operculum development, reduced bone ossification, and defects in Meckel's cartilage morphology and its underlying cellular structure. Homozygous missense larvae displayed milder cartilage defects without any major defects to early skeletal structures. Seemingly opposing phenotypes were observed in compound heterozygous zebrafish carrying the knockout and missense alleles in trans, with no obvious phenotypes seen in the Meckel's cartilage and accelerated operculum development observed. Together, our results demonstrate that different nbas variants differentially affect skeletal development, suggesting complex spectrums of phenotypic and pathogenic mechanisms in NBAS-associated atypical OI.</p>","PeriodicalId":93913,"journal":{"name":"Bone","volume":" ","pages":"118129"},"PeriodicalIF":3.6,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bound and pore water contributions to fracture in lateral femoral cortex: MRI-based extended finite element and fuzzy approximation modeling.","authors":"Ashkan Sedigh, Nada Kamona, Brandon C Jones, Brian-Tinh Vu, Christiana L Cottrell, Alyssa Rosen, Chamith S Rajapakse, Ani Ural","doi":"10.1016/j.bone.2026.118131","DOIUrl":"10.1016/j.bone.2026.118131","url":null,"abstract":"<p><p>Atypical femoral fracture (AFF) is a rare fracture associated with long-term bisphosphonate treatment that occurs in the subtrochanteric and midshaft femur. AFF is not fully explained by bone mineral density motivating identification of biomarkers of AFF risk that reflect bone quality. Cortical bone water has been linked to mechanical properties of bone. Ultra-short echo time magnetic resonance imaging (UTE MRI) can quantify water compartments in bone, including matrix-associated bound water (BW) and porosity-related pore water (PW). Here, we investigated the influence of BW and PW on fracture formation in the AFF region using MRI-based extended finite element modeling and applied fuzzy approximation to capture nonlinear effects between BW and PW on mechanical outcomes. Simulation results showed that individually, mean BW and PW were not correlated with FE-predicted whole femur fracture load or stiffness. Mean porosity index, which is the ratio of PW to total water, was negatively associated with strain at the crack site (R = -0.54, p = 0.026). Stiffness values from BW and PW-based FE simulations were significantly correlated (R = 0.92, p < 0.01), whereas no correlation was observed for fracture load. Fuzzy sensitivity analysis showed that PW and BW are interrelated variables, and their relative influence on stiffness and strain at crack location depends on their levels within the AFF region. These results provide new insights into mechanisms of AFF using MRI including the compartment-specific roles of bone water.</p>","PeriodicalId":93913,"journal":{"name":"Bone","volume":" ","pages":"118131"},"PeriodicalIF":3.6,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of the response to romosozumab in women with postmenopausal osteoporosis previously treated with denosumab.","authors":"Kazuaki Mineta, Toshihiko Nishisho, Masahiko Okada, Mitsuhiro Kamada, Koichi Sairyo","doi":"10.1016/j.bone.2026.118130","DOIUrl":"10.1016/j.bone.2026.118130","url":null,"abstract":"<p><p>Romosozumab is an antisclerostin antibody that increases bone formation and decreases bone resorption. This study aimed to identify predictors of the response to romosozumab in women with postmenopausal osteoporosis previously treated with denosumab. The study had an observational pre-post design and included 167 women with primary osteoporosis. Romosozumab 210 mg was administered subcutaneously every 4 weeks for 12 months in 87 patients who had been treated with denosumab (DMAb group) and 80 who had not (naïve group). The safety, incidence of new fractures, and changes in bone mineral density (BMD) and bone turnover markers were recorded. At 12 months, a response was defined as a ≥ 3.0% increase in BMD and a non-response as a ≥ 3.0% decrease in BMD. Non-serious adverse events occurred in six patients (3.6%) in each group. New fractures occurred in three patients (4.0%) in the DMAb group and one (1.6%) in the naïve group. At 12 months, the changes in BMD at the lumbar spine and total hip were + 12.6% and + 4.7%, respectively, in the naïve group and + 3.9% and + 0.5% in the DMAb group. Bone turnover markers increased gradually after discontinuation of denosumab. A higher P1NP at baseline was associated with a response to romosozumab and a higher TRACP-5b at 3 months and longer duration of treatment with denosumab were associated with no BMD response at the total hip (TH). Longer denosumab use (≥18 months) followed by romosozumab may be associated with reduced TH BMD response and requires validation in larger prospective studies.</p>","PeriodicalId":93913,"journal":{"name":"Bone","volume":" ","pages":"118130"},"PeriodicalIF":3.6,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Open access, China, and the changing geography of publishing in bone science: Lessons from Bone Research.","authors":"Hiroshi Kawaguchi","doi":"10.1016/j.bone.2026.118115","DOIUrl":"https://doi.org/10.1016/j.bone.2026.118115","url":null,"abstract":"<p><p>Open access has widened access to scientific knowledge while shifting much of the cost of publication from readers and libraries to authors and their funders. At the same time, China has rapidly expanded its research output and journal infrastructure, reshaping the geography of scientific publishing. This Commentary examines how these developments intersect in bone science, using Bone Research as a case study. The journal's rise reflects genuine scientific achievement as well as the strategic combination of full open access, international publishing infrastructure, a large research community, authoritative reviews, and strong representation of rapidly expanding interdisciplinary topics. Its high Journal Impact Factor should be understood as a measure of citation performance, not as evidence that every article is more rigorous or clinically consequential than work published elsewhere. These changes matter for osteoporosis research and care because publication costs and citation-oriented editorial priorities may influence which investigators, research questions, and forms of clinical evidence become visible. China did not create these rules; it adapted to them at exceptional scale and speed. A more equitable system should preserve reader access, improve transparency about article processing charges and manuscript transfers, reduce reliance on journal-level metrics, protect editorial independence from publication revenue, and support researchers with limited publication funding.</p>","PeriodicalId":93913,"journal":{"name":"Bone","volume":" ","pages":"118115"},"PeriodicalIF":3.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149120024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of trabecular microstructure using photon-counting CT: Influence of dose, spatial resolution and reconstruction algorithms.","authors":"Jaime A Peña, Felix Thomsen, Timo Damm, Sandra Freitag-Wolf, Matthias Frölich, Stefan O Schönberg, Heinz-Peter Schlemmer, Klaus Engelke, Claus-Christian Glüer, Jan-Bernd Hövener, Olav Jansen, Marc Kachelrieß, Stefan Sawall","doi":"10.1016/j.bone.2026.118066","DOIUrl":"https://doi.org/10.1016/j.bone.2026.118066","url":null,"abstract":"<p><strong>Purpose: </strong>Photon-counting CT (PCCT) for quantitative microstructural assessments of the central skeleton remains underexplored. This study evaluated the effects of radiation dose, spatial resolution, noise and reconstruction algorithm on bone mineral density (BMD), bone volume fraction (BV/TV), and trabecular separation (Tb.Sp).</p><p><strong>Materials and methods: </strong>Seven excised human vertebral bodies embedded in polymethylmethacrylate were scanned using a clinical PCCT scanner (Naeotom Alpha, Siemens Healthineers): natively at (20 mGy CTDI_{32 cm}) and within a thorax phantom at (6, 9, 12 and 15 mGy). Four repeated scans (15 mGy) served as reference. Filtered back projection (FBP) and iterative (QIR3) reconstructions were used with Br56u, Br76u and Br89u kernels. Bone parameters were estimated from cancellous volumes. Differences were tested with Wilcoxon signed-rank tests. Radiation dose impact was assessed using Bland-Altman analysis and Lin's concordance coefficient (rccc).</p><p><strong>Results: </strong>Median (IQR) ranges for BMD were 177.5 (55.1)-191.9 (57.5) mgHA/cm³, for BV/TV 0.25 (0.20)-0.47 (0.05), and Tb.Sp 0.29 (0.02)-1.50 (1.64) mm. FBP consistently yielded higher BMD and BV/TV than QIR3, albeit non-significant after statistical correction. BMD differences between algorithms were 6.6 mgHA/cm³ at 6.0 mGy Br89u, and 0.1 mgHA/cm³ for Br76u at 20 mGy. Noise was higher in FBP (range: 13-470 HU) vs QIR3 (6-124 HU). The mean absolute (%) differences to the reference across dose levels were best for Br76u/QIR3 (BMD: 0.83 mgHA/cm³ (0.5%); BV/TV: 0.01 (2.6%) and Tb.Sp: 0.79 mm (18%)). Best robustness against radiation dose changes was observed with Br56u/QIR3 and Br76u/QIR3.</p><p><strong>Conclusions: </strong>Br56u showed enlarged Tb.Sp levels despite excellent agreement, while Br89u showed increased noise levels and instability. Br76u/QIR3 showed the best balance between microstructural accuracy, image noise and stability at clinically diagnostic dose levels, for example 15 mGy CTDIvol.</p>","PeriodicalId":93913,"journal":{"name":"Bone","volume":" ","pages":"118066"},"PeriodicalIF":3.6,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148835253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the editor concerning 'Impact of diabetes on the risk of subsequent fractures in 92,600 patients with an incident hip fracture: A Danish nationwide cohort study 2004-2018'.","authors":"Junqing Miao, Xiaole Kong, Jingzhi Wang","doi":"10.1016/j.bone.2024.117124","DOIUrl":"https://doi.org/10.1016/j.bone.2024.117124","url":null,"abstract":"","PeriodicalId":93913,"journal":{"name":"Bone","volume":"38 23","pages":"117124"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141045049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Swim training induces distinct osseous gene expression pattern in anosteocytic and osteocytic teleost fish.","authors":"Josephine T Tauer, Tobias Thiele, Catherine Julien, Lior Ofer, P. Zaslansky, Ron Shahar, Bettina M. Willie","doi":"10.1016/j.bone.2024.117125","DOIUrl":"https://doi.org/10.1016/j.bone.2024.117125","url":null,"abstract":"","PeriodicalId":93913,"journal":{"name":"Bone","volume":"210 S650","pages":"117125"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141040215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of synbiotics-glyconutrients on growth performance, nutrient digestibility, gas emission, meat quality, and fatty acid profile of finishing pigs.","authors":"Olivier Munezero, Sungbo Cho, In Ho Kim","doi":"10.5187/jast.2023.e52","DOIUrl":"10.5187/jast.2023.e52","url":null,"abstract":"<p><p>Glyconutrients help in the body's cell communication. Glyconutrients and synbiotics are promising options for improving immune function. Therefore, we hypothesized that combining synbiotics and glyconutrients will enhance pig nutrient utilization. 150 pigs (Landrace × Yorkshire × Duroc), initially weighing 58.85 ± 3.30 kg of live body weight (BW) were utilized to determine the effects of synbiotics-glyconutrients (SGN) on the pigs' performance, feed efficiency, gas emission, pork traits, and composition of fatty acids. The pigs were matched by BW and sex and chosen at random to 1 of 3 diet treatments: control = Basal diet; TRT1 = Basal diet + SGN 0.15%; TRT2 = Basal diet + SGN 0.30%%. The trials were conducted in two phases (weeks 1-5 and weeks 5-10). The average daily gain was increased in pigs fed a basal diet with SGN (<i>p</i> = 0.036) in weeks 5-10. However, the apparent total tract digestibility of dry matter, nitrogen, and gross energy did not differ among the treatments (<i>p</i> > 0.05). Dietary treatments had no effect on NH₃, H₂S, methyl mercaptans, acetic acids, and CO₂ emissions (<i>p</i> > 0.05). Improvement in drip loss on day 7 (<i>p</i> = 0.053) and tendency in the cooking loss were observed (<i>p</i> = 0.070) in a group fed basal diets and SGN at 0.30% inclusion level. The group supplemented with 0.30% of SGN had higher levels of palmitoleic acid (C16:1), margaric acid (C17:0), omega-3 fatty acid, omega-6 fatty acid, and ω-6: ω-3 ratio (<i>p</i> = 0.034, 0.020, 0.025, 0.007, and 0.003, respectively) in the fat of finishing pigs. Furthermore, group supplemented with 0.30% of SGN improved margaric acid (C17:0), linoleic acid (C18:2n6c), arachidic acid (C20:0), omega 6 fatty acid, omega-6 to omega-3 ratio, unsaturated fatty acid, and monounsaturated fatty acid (p = 0.037, 0.05, 0.0142, 0.036, 0.033, 0.020, and 0.045, respectively) in the lean tissues of finishing pigs compared to pigs fed with the control diets. In conclusion, the combination of probiotics, prebiotics, and glyconutrients led to higher average daily gain, improved the quality of pork, and more favorable fatty acid composition. Therefore, these results contributed to a better understanding of the potential of SGN combinations as a feed additive for pigs.</p>","PeriodicalId":93913,"journal":{"name":"Bone","volume":"29 1","pages":"310-325"},"PeriodicalIF":2.3,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11016742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78886618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TNF-α promotes osteocyte necroptosis by upregulating TLR4 in postmenopausal osteoporosis","authors":"Hongwang Cui, Ji Li, Xiangtao Li, Tian Su, Peng Wen, Chuanling Wang, Xiaozhong Deng, Yonghua Fu, Weijie Zhao, Changjia Li, Pengbing Hua, Yongjun Zhu, Wei Wan","doi":"10.1016/j.bone.2024.117050","DOIUrl":"https://doi.org/10.1016/j.bone.2024.117050","url":null,"abstract":"","PeriodicalId":93913,"journal":{"name":"Bone","volume":"878 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139831347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}