Animal models and experimental medicine最新文献_第2页

Brassinin from Brassica campestris L. inhibits colorectal cancer by inducing p62/NRF2/GPX4-regulated ferroptosis.

Animal models and experimental medicine Pub Date : 2025-01-23 DOI: 10.1002/ame2.12521

Shi-Yuan Wen, Rui-Rui Gao, Yan-Yan Chen, Yi-Jie Wang, Xin-Tong Wang, Hai-Xin Liu

{"title":"Brassinin from Brassica campestris L. inhibits colorectal cancer by inducing p62/NRF2/GPX4-regulated ferroptosis.","authors":"Shi-Yuan Wen, Rui-Rui Gao, Yan-Yan Chen, Yi-Jie Wang, Xin-Tong Wang, Hai-Xin Liu","doi":"10.1002/ame2.12521","DOIUrl":"https://doi.org/10.1002/ame2.12521","url":null,"abstract":"Background: Indole phytoalexins, plant-derived compounds present in cruciferous vegetables, have demonstrated anticancer properties. Brassinin (BSN), derived from Brassica campestris L. var. campestris, is known for its potent antitumor effects on various cancers. However, the role of ferroptosis in regulating the antitumor effects of BSN has not been fully elucidated.Methods: The components of B. campestris L. against colorectal cancer (CRC) were analyzed by network pharmacology. CCK-8 assay and colony formation assay detected cell viability induced by BSN. Molecular docking verified the binding of BSN to the target protein. Western blot and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay revealed whether BSN can inactivate the NRF2 signaling and inhibit the expression of p62 and HO-1. The RKO-xenograft tumor models were established and then were treated by 75 or 150 mg/kg BSN to verify the antitumor efficacy and side effects of BSN.Results: Network pharmacology suggested that BSN is the most important component of B. campestris L. against CRC. BSN inhibits CRC cell viability in a dose- and time-dependent manner. Furthermore, this inhibitory effect is associated with the induction of ferroptosis, as BSN suppresses the cell viability of CRC by inducing GPX4-regulated ferroptosis. BSN may bind to NRF2 protein to inactivate the NRF2 signaling, inhibiting the expression of p62 and HO-1. Importantly, a low dose or a high dose of BSN significantly reduced the tumor growth in vivo.Conclusions: Our findings reveal that BSN blocks CRC growth by inducing p62/NRF2/GPX4-regulated ferroptosis, which may be a novel lead compound for tumor treatment.","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Animal models of cisplatin-induced neuropathic pain.

Animal models and experimental medicine Pub Date : 2025-01-23 DOI: 10.1002/ame2.12548

Ningxin Li, Mingzhu Li, Shengbo Jin, Jun Yu, Hongzhe Wei, Wenping Wang, Siyao Ma, Yuxin Jiang, Qian Liu, Huini Yao

引用次数: 0

Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model.

Animal models and experimental medicine Pub Date : 2025-01-23 DOI: 10.1002/ame2.12549

Ping Tang, Yi Sun, Chunsheng Yang, Nan Zhang

{"title":"Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model.","authors":"Ping Tang, Yi Sun, Chunsheng Yang, Nan Zhang","doi":"10.1002/ame2.12549","DOIUrl":"https://doi.org/10.1002/ame2.12549","url":null,"abstract":"Background: Subcortical ischemic vascular dementia (SIVD) is a common subtype of vascular dementia. Currently, the bilateral common carotid artery stenosis (BCAS) mouse model is the most suitable SIVD rodent model. In this study, we investigated the functional and structural impairments in the hippocampus 1 month after BCAS.Methods: We used behavioral tests, laser speckle flowmetry, long-term potentiation, histochemical staining, molecular experiments, and voxel-based morphometry to evaluate the hippocampal impairments.Results: Behavioral studies revealed that BCAS mice exhibited worse performance. Laser speckle flowmetry detected an obvious decrease in cerebral blood flow. The synaptic plasticity of the perforant path-dentate gyrus pathway was inhibited. Decreased fractional anisotropy and increased mean diffusivity were detected in the hippocampus via diffusion tensor imaging data. A reduction in gray matter volume, which was most prominent in the hippocampus and its surrounding areas, was detected via voxel-based morphometry analysis. Impairments in cell morphology and myelin integrity were validated using histochemical staining and molecular biology techniques. In addition, the numbers of GFAP+ astrocytes and Iba1+ microglia increased in the hippocampus.Conclusions: Overall, our study demonstrates early functional and structural impairments in the hippocampus contributing to learning and memory deficits after 1 month of BCAS, indicating that the hippocampus is vulnerable to chronic cerebral ischemia.","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectively simplified Adriamycin-induced chronic kidney disease mouse model: Retro-orbital vein injection versus tail-vein injection.

Animal models and experimental medicine Pub Date : 2025-01-22 DOI: 10.1002/ame2.12553

Masaki Watanabe, Hayato R Takimoto, Kazuki Hashimoto, Yuki Ishii, Nobuya Sasaki

{"title":"Effectively simplified Adriamycin-induced chronic kidney disease mouse model: Retro-orbital vein injection versus tail-vein injection.","authors":"Masaki Watanabe, Hayato R Takimoto, Kazuki Hashimoto, Yuki Ishii, Nobuya Sasaki","doi":"10.1002/ame2.12553","DOIUrl":"https://doi.org/10.1002/ame2.12553","url":null,"abstract":"This study aimed to investigate the impact of administration routes in establishing the Adriamycin (ADR)-induced chronic kidney disease (CKD) model. Using BALB/c mice, we compared the effects of conventional tail-vein injection (TV10, 10 mg/kg) to those of retro-orbital sinus (orbital vein) injection (OV10, 10 mg/kg; OV8, 8 mg/kg). The results indicated that the OV10 group exhibited CKD pathology similar to the TV10 group, with both groups demonstrating significantly higher urinary albumin/creatinine ratio (p < 0.05), tubular injury (p < 0.05), and degree of renal fibrosis (p < 0.05) than the OV8 group. No significant differences were observed between the OV10 and TV10 groups in urinary albumin/creatinine ratio, tubular injury, and degree of renal fibrosis. These findings demonstrated that retro-orbital administration of 10 mg/kg ADR induces comparable effects to conventional tail-vein administration. This technique's technical simplicity may improve experimental efficiency, reproducibility, and animal welfare in CKD research. In conclusion, this study validates the utility of retro-orbital injection in CKD model establishment, demonstrating its potential to standardize and improve the reliability of future CKD research protocols.","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting signaling pathways in neurodegenerative diseases: Quercetin's cellular and molecular mechanisms for neuroprotection.

Animal models and experimental medicine Pub Date : 2025-01-22 DOI: 10.1002/ame2.12551

Md Rezaul Islam, Md Ibrahim Khalil Al-Imran, Mehrukh Zehravi, Sherouk Hussein Sweilam, Mohammad Rakib Mortuza, Jeetendra Kumar Gupta, Thukani Sathanantham Shanmugarajan, Kadirvel Devi, Tanuja Tummala, Mohammed Ali Alshehri, Kalirajan Rajagopal, Mohammed Asiri, Irfan Ahmad, Talha Bin Emran

{"title":"Targeting signaling pathways in neurodegenerative diseases: Quercetin's cellular and molecular mechanisms for neuroprotection.","authors":"Md Rezaul Islam, Md Ibrahim Khalil Al-Imran, Mehrukh Zehravi, Sherouk Hussein Sweilam, Mohammad Rakib Mortuza, Jeetendra Kumar Gupta, Thukani Sathanantham Shanmugarajan, Kadirvel Devi, Tanuja Tummala, Mohammed Ali Alshehri, Kalirajan Rajagopal, Mohammed Asiri, Irfan Ahmad, Talha Bin Emran","doi":"10.1002/ame2.12551","DOIUrl":"https://doi.org/10.1002/ame2.12551","url":null,"abstract":"Background: Neurodegenerative diseases (NDs), including Alzheimer's disease, Parkinson's disease, and Huntington's disease, are complex and challenging due to their intricate pathophysiology and limited treatment options.Methods: This review systematically sourced articles related to neurodegenerative diseases, neurodegeneration, quercetin, and clinical studies from primary medical databases, including Scopus, PubMed, and Web of Science.Results: Recent studies have included quercetin to impact the cellular and molecular pathways involved in neurodegeneration. Quercetin, a flavonoid abundant in vegetables and fruits, is gaining attention for its antioxidant, anti-inflammatory, and antiapoptotic properties. It regulates signaling pathways such as nuclear factor-κB (NF-κB), sirtuins, and phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt). These pathways are essential for cellular survival, inflammation regulation, and apoptosis. Preclinical and clinical studies have shown that quercetin improves symptoms and pathology in neurodegenerative models, indicating promising outcomes.Conclusions: The study explores the potential of incorporating laboratory research into practical medical treatment, focusing on quercetin's neuroprotective effects on NDs and its optimal dosage.","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mouse coccygeal intervertebral disc degeneration model with tail-looping constructed using a suturing method. 采用缝合法建立小鼠尾椎椎间盘退变伴尾环模型。

Animal models and experimental medicine Pub Date : 2025-01-14 DOI: 10.1002/ame2.12501

Wei Xie, Zemao Huang, Ziyang Huang, Deqing Luo, Zhangxin Chen, Li Xie, Lingqi Zhu, Hui Liu, Kejian Lian, Paolo Alberton, Denitsa Docheva, Dasheng Lin

{"title":"A mouse coccygeal intervertebral disc degeneration model with tail-looping constructed using a suturing method.","authors":"Wei Xie, Zemao Huang, Ziyang Huang, Deqing Luo, Zhangxin Chen, Li Xie, Lingqi Zhu, Hui Liu, Kejian Lian, Paolo Alberton, Denitsa Docheva, Dasheng Lin","doi":"10.1002/ame2.12501","DOIUrl":"https://doi.org/10.1002/ame2.12501","url":null,"abstract":"Backgroud: Intervertebral disc degeneration (IDD) is one of the common degenerative diseases. Due to ethical constraints, it is difficult to obtain sufficient research on humans, so the use of an animal model of IDD is very important to clarify the pathogenesis and treatment mechanism of the disease.Methods: In this study, thirty 2-month-old mice were selected for operation to establish a coccygeal IDD model. The distal tail portion of the tail (beyond the 17th coccygeal vertebra) and a small piece of skin above the 8th coccygeal vertebra were excised, and the two incisions were brought together after flexion, and secured with sutures. The heights and signal intensities of the intervertebral discs (IVDs) were assessed using microcomputed tomography (μCT) and magnetic resonance imaging (MRI) at 0, 6, 12 weeks postoperatively. The overall tissue morphology, cell distribution and density, and extracellular matrix of the IVDs were also assessed using Hematoxylin and Eosin (HE), Safranin O-Fast Green and immunohistochemical staining.Results: All mice in the experimental group survived after the operation, and there were no complications such as wound infection, tail necrosis and suture shedding. The experimental results demonstrated that the suturing method can successfully initiate IDD. Different severity levels of IDD can be induced by controlling the bending angle of the IVDs within the tail loop; however, for consistency, histologic and imaging results should be obtained at the same bending angle and looping period.Conclusions: This IDD model is an effective method for studying the etiology and treatment of degenerative IVD disease.","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A study on the variation of cytokine and electrolyte levels in rhesus macaques, cynomolgus monkeys, and Assamese macaques. 关于猕猴、眼镜猴和阿萨姆猕猴细胞因子和电解质水平变化的研究。

Animal models and experimental medicine Pub Date : 2025-01-14 DOI: 10.1002/ame2.12500

Suqin Duan, Jinghan Hou, Yanyan Li, Wenting Sun, Mingxue Li, Lixiong Chen, Hongjie Xu, Weihua Jin, Quan Liu, Jie Tang, Zijun Deng, Fengmei Yang, Zhanlong He

{"title":"A study on the variation of cytokine and electrolyte levels in rhesus macaques, cynomolgus monkeys, and Assamese macaques.","authors":"Suqin Duan, Jinghan Hou, Yanyan Li, Wenting Sun, Mingxue Li, Lixiong Chen, Hongjie Xu, Weihua Jin, Quan Liu, Jie Tang, Zijun Deng, Fengmei Yang, Zhanlong He","doi":"10.1002/ame2.12500","DOIUrl":"https://doi.org/10.1002/ame2.12500","url":null,"abstract":"Background: Non-human primates (NPHs), such as rhesus macaques, cynomolgus monkeys, and Assamese macaques, play a crucial role in biomedical research. However, baseline cytokine and electrolyte data for these three species, particularly data stratified by age and sex, are limited. Therefore, the aim of this study was to establish and analyze age- and sex-specific cytokine and electrolyte profiles in these three species.Methods: This study included 40 rhesus macaques (21 males, 19 females), 33 cynomolgus monkeys (17 males, 16 females), and 45 Assamese macaques (25 males, 20 females) classified by age (1-5 years, 6-12 years, >13 years) and sex. The levels of 23 immune function indicators and 5 electrolyte indicators were measured.Results: Among the three monkey species, the levels of sCD40L, IL-18, MCP-1, MIP-1β, TGFa, K+, Na+, and Cl- exhibited species-, sex-, and age-related differences. Comparison within the same species，sex had no significant impact on cytokine levels in NHPs but did affect electrolyte levels, particularly Cl- and Na+ levels, in cynomolgus monkeys and Assamese macaques. Electrolyte levels in NHPs were not affected by age, whereas the levels of certain cytokines, particularly sCD40L, GM-CSF, and IL-10, varied with age. The remaining 21 cytokines demonstrated no significant age-related changes.Conclusions: Significant variations in cytokine and electrolyte levels exist among different monkey species, sexes, and age groups. This research provides valuable resources for NHP researchers and sets the stage for further exploring the impacts of sex and age on NHP physiology and immune function.","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the neuroprotective benefits of phytochemicals extracted from indigenous edible fruits in Bangladesh. 探索从孟加拉国本土可食用水果中提取的植物化学物质的神经保护作用。

Animal models and experimental medicine Pub Date : 2025-01-14 DOI: 10.1002/ame2.12522

Sumon Roy, Sajib Chandra Roy, Mehrukh Zehravi, Sherouk Hussein Sweilam, Rajib Das, Mylsamy Palanisamy, Venkata Lakshamana Sagar Dantinapalli, Selvaraja Elumalai, Jeetendra Kumar Gupta, Mohammed Ali Alshehri, Mohammed Asiri, Irfan Ahmad, Mohamed H Nafady, Talha Bin Emran

{"title":"Exploring the neuroprotective benefits of phytochemicals extracted from indigenous edible fruits in Bangladesh.","authors":"Sumon Roy, Sajib Chandra Roy, Mehrukh Zehravi, Sherouk Hussein Sweilam, Rajib Das, Mylsamy Palanisamy, Venkata Lakshamana Sagar Dantinapalli, Selvaraja Elumalai, Jeetendra Kumar Gupta, Mohammed Ali Alshehri, Mohammed Asiri, Irfan Ahmad, Mohamed H Nafady, Talha Bin Emran","doi":"10.1002/ame2.12522","DOIUrl":"https://doi.org/10.1002/ame2.12522","url":null,"abstract":"The increasing incidence of neurodegenerative diseases (NDs) and the constraints of existing treatment methods have spurred a keen interest in investigating alternative therapies. Medicinal plants, renowned for their long-standing use in traditional medicine, offer a hopeful avenue for discovering new neuroprotective agents. This study emphasizes the potential neuroprotective characteristics of edible fruit plants in Bangladesh, specifically focusing on their traditional folk medicine uses for neurological disorders. This study provides an in-depth overview of the different types of edible fruit trees in Bangladesh and their phytochemicals, including flavonoids, terpenoids, and phenolic acids. This work examines the scientific data supporting the neuroprotective properties of bioactive chemicals from plants. It further explores the mechanisms by which these compounds work to counteract oxidative stress, decrease inflammation, and stimulate neurogenesis. Moreover, the study investigates toxicological characteristics and bioactive components of some fruits, emphasizing the importance of further investigation to measure their safety profile comprehensively. This thorough study highlights the potential benefits of Bangladesh's edible fruit trees as a rich source of neuroprotective chemicals. It also shows that additional research might lead to novel approaches for improving brain functioning and preventing NDs.","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenols as Wnt/β-catenin pathway modulators: A promising strategy in clinical neurodegeneration. 多酚作为 Wnt/β-catenin 通路调节剂：临床神经退行性病变的前景看好的策略。

Animal models and experimental medicine Pub Date : 2025-01-14 DOI: 10.1002/ame2.12525

Biswajit Kumar Utpal, Sajib Chandra Roy, Mehrukh Zehravi, Sherouk Hussein Sweilam, A Dinesh Raja, M Akiful Haque, Chandan Nayak, Senthilkumar Balakrishnan, Laliteshwar Pratap Singh, Saswati Panigrahi, Mohammed Ali Alshehri, Safia Obaidur Rab, Najmus Sakib Minhaj, Talha Bin Emran

{"title":"Polyphenols as Wnt/β-catenin pathway modulators: A promising strategy in clinical neurodegeneration.","authors":"Biswajit Kumar Utpal, Sajib Chandra Roy, Mehrukh Zehravi, Sherouk Hussein Sweilam, A Dinesh Raja, M Akiful Haque, Chandan Nayak, Senthilkumar Balakrishnan, Laliteshwar Pratap Singh, Saswati Panigrahi, Mohammed Ali Alshehri, Safia Obaidur Rab, Najmus Sakib Minhaj, Talha Bin Emran","doi":"10.1002/ame2.12525","DOIUrl":"https://doi.org/10.1002/ame2.12525","url":null,"abstract":"Polyphenols, a diverse group of naturally occurring compounds found in plants, have garnered significant attention for their potential therapeutic properties in treating neurodegenerative diseases (NDs). The Wnt/β-catenin (WβC) signaling pathway, a crucial player in neurogenesis, neuronal survival, and synaptic plasticity, is involved in several cellular mechanisms related to NDs. Dysregulation of this pathway is a hallmark in the development of various NDs. This study explores multiple polyphenolic compounds, such as flavonoids, stilbenes, lignans, and phenolic acids, and their potential to protect the nervous system. It provides a comprehensive analysis of their effects on the WβC pathway, elucidating their modes of action. The study highlights the dual function of polyphenols in regulating and protecting the nervous system, providing reassurance about the research benefits. This review provides a comprehensive analysis of the results obtained from both in vitro studies and in vivo research, shedding light on how these substances influence the various components of the pathway. The focus is mainly on the molecular mechanisms that allow polyphenols to reduce oxidative stress, inflammation, and apoptotic processes, ultimately improving the function and survival of neurons. This study aims to offer a thorough understanding of the potential of polyphenols in targeting the WβC signaling pathway, which could lead to the development of innovative therapeutic options for NDs.","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A canine model of reversible urethral sphincter insufficiency. 犬可逆性尿道括约肌功能不全模型。

Animal models and experimental medicine Pub Date : 2025-01-03 DOI: 10.1002/ame2.12519

Pourya Shokri, Shahin Tabatabaei, Seyed Amir Mohsen Ziaee, Mir Sepehr Pedram, Mohammad Mehdi Dehghan, Nasser Shakhssalim

{"title":"A canine model of reversible urethral sphincter insufficiency.","authors":"Pourya Shokri, Shahin Tabatabaei, Seyed Amir Mohsen Ziaee, Mir Sepehr Pedram, Mohammad Mehdi Dehghan, Nasser Shakhssalim","doi":"10.1002/ame2.12519","DOIUrl":"https://doi.org/10.1002/ame2.12519","url":null,"abstract":"This study developed an animal model with internal and external urethral sphincter insufficiency by bypassing the sphincter without major damage so that the animal under study can return to normal life after the study. There is a need for a reliable, applicable, and reproducible animal model for studying urinary incontinency disease due to incorrect sphincter function. Seven adult male dogs were used for this study. The urethral sphincter was bypassed by inserting a catheter between the bladder neck and the distal sphincter. The animals' physical condition was closely monitored for 9 weeks, and standard urodynamic and radiologic studies were performed before and 1-2 months after surgery. The animals were killed at 9 weeks after surgery for pathological assessment. Catheter placement caused complete incontinence in the animal, with urodynamic assessments indicating that the animal was unable to control urination and radiological assessments indicating an empty bladder with a residual volume of 50 ± 10 cc. Tissue analysis did not show significant histological damage and inflammation. The study shows that by bypassing the urethral sphincter, which is a reliable and reproducible method, an animal model of urinary incontinence can be developed, which can be used in various studies such as assessing the adequacy of artificial sphincter function. The animals under study did not have any permanent defect, so they were able to return to their normal life.","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0