Animal models and experimental medicine最新文献

{"title":"Comparative analysis of two newly established Cre rat lines, NeuN-Cre and Thy1-Cre, for neurological research.","authors":"Keru Li, Chenyang Liu, Gefan Wan, Lei Zhang, Rui Fan, Xu Zhang, Yuanlong Su, Jiayue He, Ning Liu, Feifei Guan, Wei Dong, Shan Gao, Weining Kong, Xiaolong Qi, Xiaolin Zhang, Yuanwu Ma","doi":"10.1002/ame2.70051","DOIUrl":"https://doi.org/10.1002/ame2.70051","url":null,"abstract":"<p><strong>Background: </strong>The Cre/loxP system is most popular in mice, but its application in rats has largely lagged far behind. The rat is vital laboratory animal, especially in toxicological and neurological studies. Generating genetic tools to manipulate neurons in rats could benefit neurological research.</p><p><strong>Methods: </strong>Using the CRISPR/Cas9 system, we inserted a Cre cassette into endogenous Thy1 and NeuN loci. Thy1-Cre rats featured a downstream P2A-linked insertion, while NeuN-Cre was inserted at the transcriptional start site. The Cre activity was assessed by crossing with a Cre reporter (Rosa26^imCherry) rat and through analyzing mCherry expression patterns. The specificity of cell type was further confirmed by immunofluorescence with NeuN antibody. Phenotypic consequences were assessed by crossing with ND1^LSL rats to deplete ND1, followed by monitoring weight/survival and conducting motor function tests.</p><p><strong>Results: </strong>We generated two neuron-specific rats (Thy1-Cre and NeuN-Cre), which exhibited high neuron-specific Cre expression in brain and spinal cord with minor leakage in other tissues. Thy1-Cre showed minor leakage in spleen, lung and kidney while NeuN-Cre showed minor leakage in spleen and kidney. ND1^Thy1-Cre and ND1^NeuN-Cre rats both showed decreased body weights and survival times. The ND1^NeuN-Cre rats died within two weeks, while ND1^Thy1-Cre rats lived longer with impaired motor function.</p><p><strong>Conclusions: </strong>We successfully generated two neuron-specific NeuN-Cre and Thy1-Cre rats, and systemically analyzed their expression pattern.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advances in dysphagia animal models.","authors":"Junhui Bai, Keling Cheng, Nannan Zhang, Yunfang Chen, Jun Ni, Zhiyong Wang","doi":"10.1002/ame2.70054","DOIUrl":"https://doi.org/10.1002/ame2.70054","url":null,"abstract":"<p><p>Dysphagia is a common complication of stroke, Parkinson's disease (PD), and amyotrophic lateral sclerosis (ALS). The construction of animal models of dysphagia is an important way to explore its pathogenesis and treatment. At present, the animal models of dysphagia mainly include rodents, nonhuman primates, and other mammals, such as pigs and dogs. This review systematically summarizes the establishment and evaluation of dysphagia animal models in stroke, PD, and ALS in three kinds of experimental animals, providing a basis for the selection of appropriate animal models of dysphagia.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scleral reactions to different suture materials: A comparative quantitative histological study in a rabbit model","authors":"Lenka Vaňková,&nbsp;Věra Křížková,&nbsp;Martina Grajciarová,&nbsp;Veronika Hátlová,&nbsp;Lenka Hecová,&nbsp;Markéta Štefková,&nbsp;Jiří Cendelín,&nbsp;Pavel Klein,&nbsp;Štěpán Rusňák,&nbsp;Pavel Studený","doi":"10.1002/ame2.70036","DOIUrl":"10.1002/ame2.70036","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Scleral fixation of intraocular lenses is a surgical technique that involves anchoring an artificial lens to the sclera. Traditional approaches, such as capsular bag placement, may not be feasible in certain situations, making scleral fixation a valuable alternative. The scleral reactions to different types of suture materials are not fully understood. Therefore, the present study describes the microscopic structure of normal scleral tissue and its changes with suture materials.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We compared six groups of rabbit eyes focusing on the sclera: group with polytetrafluoroethylene (PTFE) chain, PTFE fiber, polypropylene (PPE) fiber and control groups. multilevel sampling and stereological methods were used for histological quantification of the leukocyte infiltration fractions and type I and type III collagen.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Quantitative histological evaluation revealed the following: (1) For all materials used, inflammation was present in the surrounding scleral tissue compared with healthy controls. However, leukocyte infiltration in the sclera was not statistically different between the materials. (2) As part of the evaluation of collagen, the greatest changes occurred in the PTFE fiber group at 2 weeks postoperatively. In the PTFE chain group, more significant changes were visible at 4 weeks. (3) The changes in the PPE fiber group compared to healthy scleral tissue were the least significant.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>From a histological point of view, it is evident that there are differences in the quantitative parameters between the untouched sclera and the sclera with suture material. Furthermore, distinctions were observed among various materials and across different time intervals.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 6","pages":"1119-1129"},"PeriodicalIF":0.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.70036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a humanized SCA2 mouse model carrying a CAA disruption preventing CAG repeat expansion in pathogenic genes.","authors":"Yao Zhang, Yufei Li, Lin Zhang, Zhaoqing Li, Keqin Lin, Kai Huang, Zhaoqing Yang, Shaohui Ma, Hao Sun, Xiaochao Zhang","doi":"10.1002/ame2.70047","DOIUrl":"https://doi.org/10.1002/ame2.70047","url":null,"abstract":"<p><strong>Background: </strong>Spinocerebellar ataxia type 2 (SCA2) is a neurodegenerative disease marked by significant clinical and genetic heterogeneity, primarily caused by expanded CAG mutations in the ATXN2 gene. The unstable expansion of CAG repeats disrupts the genetic stability of animal models, which is detrimental to disease research.</p><p><strong>Methods: </strong>In this study, we established a mouse model in which CAG repeats do not undergo microsatellite instability (MSI) across generations. A humanized ATXN2 cDNA with four CAA interruptions within 73 CAG expansions was inserted into the Rosa26 locus of C57BL/6J mice. A 23 CAG control mouse model was also generated to verify ATXN2 integration and expression.</p><p><strong>Results: </strong>In our model, the number of CAG repeats remained stable during transmission, with no CAG repeat expansion observed in 64 parent-to-offspring transmissions. Compared with SCA2-Q23 mice, SCA2-Q73 mice exhibited progressive motor impairment, reduced Purkinje cell count and volume (indicative of cell atrophy), and muscle atrophy. These observations in the mice suggest that the behavioral and neuropathological phenotypes may reflect the features of SCA2 patients. RNA-seq analysis of the gastrocnemius muscle in SCA2-Q73 mice showed significant changes in muscle differentiation and development gene expression at 56 weeks, with no significant differences at 16 weeks compared to SCA2-Q23 mice. The expression level of the Myf6 gene significantly changed in the muscles of aged mice.</p><p><strong>Conclusion: </strong>In summary, the establishment of this model not only provides a stable animal model for studying CAG transmission in SCA2 but also indicates that the lack of long-term neural stimulation leads to muscle atrophy.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple low dose streptozotocin-induced diabetes as a model for studying autoimmune diabetes in humans.","authors":"Ivan Koprivica, Suzana Stanisavljević, Dragica Mićanović, Ivana Stojanović, Đorđe Miljković","doi":"10.1002/ame2.70050","DOIUrl":"https://doi.org/10.1002/ame2.70050","url":null,"abstract":"<p><p>The autoimmune response directed against pancreatic β cells is the most essential pathogenic process in type 1 diabetes (T1D) in humans. Spontaneous animal models of T1D greatly contribute to our understanding of the disease pathogenesis and therapeutic options. Amongst many disease models, a significant proportion of T1D research is performed on multiple low dose streptozotocin induced diabetes in experimental animals, in parallel. Here, we discuss advantages of this model for contemporary T1D research. Additionally, challenges and perspectives for further improvement of the model are presented.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and evaluation of a novel rat model of the fourth ventricle hemorrhage.","authors":"Chuhua Fu, Aoqi Liu, Yijia He, Fei Li, Zhouyang Jiang, Peiwen Guo, Zhi Chen, Yujie Chen, Hua Feng","doi":"10.1002/ame2.70049","DOIUrl":"https://doi.org/10.1002/ame2.70049","url":null,"abstract":"<p><strong>Background: </strong>Hemorrhagic expansion into the fourth ventricle is an independent risk factor for poor outcomes in intraventricular hemorrhage (IVH) patients. However, to date, available animal models of IVH are limited to models of supratentorial ventricular hemorrhage, and there are no specific models of fourth ventricle hemorrhage. This limitation hinders comprehensive basic research and the understanding of the pathophysiological changes that occur following fourth ventricle hemorrhage. Therefore, the development of an animal model of fourth ventricle hemorrhage is highly important.</p><p><strong>Methods: </strong>In this study, a novel rat model of fourth ventricle hemorrhage was established via autologous blood injection through the foramen of Magendie. Anesthetized rats were positioned in a stereotaxic apparatus with their heads tilted downward at an angle of approximately 20° relative to the vertical axis. A needle was inserted through the foramen, and autologous blood obtained from the rat's heart was injected into the fourth ventricle via a microinfusion pump. Systematic evaluations of the model were conducted using small-animal magnetic resonance imaging, histopathological analysis, and neurological function assessment.</p><p><strong>Results: </strong>The rats developed stable and reproducible fourth ventricle hematomas and ventricular dilation. They also exhibited acute-phase hydrocephalus and pathological features of perilesional brain tissue injury, with observed neurological deficits comparable to patients with fourth ventricle hemorrhage.</p><p><strong>Conclusion: </strong>This model successfully recapitulates the clinicopathological and pathophysiological characteristics of patients with fourth ventricle hemorrhage and can be utilized for further investigation into the pathophysiological mechanisms underlying posthemorrhagic hydrocephalus and perilesional brainstem tissue injury.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a novel alloxan-induced rabbit model exhibiting unique diabetic retinal neuropathy features assessed via ERG + VEP.","authors":"Xinlu Li, Xiaojing Dong, Defei Feng, Han Hu, Bai Li, Zhongjian Liu, Wei He, Chenchen Huang, Zhizhou Shi, Yan Mei","doi":"10.1002/ame2.70032","DOIUrl":"https://doi.org/10.1002/ame2.70032","url":null,"abstract":"<p><strong>Background: </strong>Diabetic retinal neuropathy (DRN) leads to significant visual impairment; however, no existing animal model fully replicates its neural alterations, and inconsistent induction protocols with high mortality rates hinder long-term investigations.</p><p><strong>Methods: </strong>Adult male rabbits were randomly assigned to four experimental groups, each receiving a single intravenous injection of varying doses of alloxan and one control group. The safety and efficacy of alloxan in inducing diabetes were evaluated to determine the optimal dose. At 9 weeks following injection with alloxan, retinal function was assessed using full-field electroretinography (ERG) and visual evoked potentials (VEPs). Retinal structure was examined in rabbits using spectral-domain optical coherence tomography (SD-OCT), Optos ultra-widefield (Optos UWF) false-color imaging, and widefield fundus fluorescein angiography (WF-FFA).</p><p><strong>Results: </strong>Rabbits in the 80 mg/kg alloxan group exhibited fewer complications, lower mortality, and a higher model success rate compared to other groups. At 9 weeks post-injection, these rabbits demonstrated significantly elevated hemoglobin A1c and total cholesterol (p < 0.05) relative to controls. ERG revealed statistically significant reductions in oscillatory potential and b-wave amplitudes (p < 0.05), while VEP indicated decreased P2 amplitude (p < 0.001) and prolonged P2 latency (p < 0.05). SD-OCT, Optos UWF imaging, and WF-FFA demonstrated no significant changes in vascular abnormalities. Additionally, Hematoxylin and Eosin staining revealed retinal swelling (p < 0.05), and immunofluorescence confirmed glial activation and neuronal loss.</p><p><strong>Conclusions: </strong>A single intravenous injection of 80 mg/kg alloxan effectively and safely induced DRN in rabbits, resulting in neural retina damage, thereby establishing this model as an ideal model for DRN research.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of allicin against stanozolol-induced cardiotoxicity: Physiological and histopathological evidence in a rabbit model.","authors":"Mohammed Hayder Asker, Noor Al-Huda Salah Al-Zuhairy, Wassan Mhammed Husain, Mustafa Riyadh Abdullah","doi":"10.1002/ame2.70035","DOIUrl":"https://doi.org/10.1002/ame2.70035","url":null,"abstract":"<p><strong>Background: </strong>There are many forms of anabolic steroids, including stanozolol (Winstrol), which are popular for their muscle-building effects but dangerous to the heart. This present work is aimed at evaluating the pharmacologica impact of allicin, a natural attribute obtained from garlic, on obstructing cardiac injury in rabbits that received stanozolol.</p><p><strong>Methods: </strong>Thirty rabbits were divided into three groups: control, stanozolol-treated, and stanozolol plus allicin. Cardiac function was assessed by measuring troponin, creatine kinase (CK), Galectin-3, and GDF-15. Oxidative stress and antioxidant markers, including malondialdehyde (MDA), glutathione, and catalase, were analyzed. Inflammatory mediators such as C-reactive protein (CRP), interleukin-6 (IL-6), NF-κB, iNOS, nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) were evaluated. Lipid profile parameters, including total cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein (HDL), were measured. Histopathological examination assessed myocardial damage, fibrosis, and collagen deposition.</p><p><strong>Results: </strong>Stanozolol administration significantly increased cardiac damage markers, oxidative stress, and inflammatory mediators while causing dyslipidemia, characterized by elevated LDL and total cholesterol and reduced HDL. Allicin co-administration effectively countered these effects by reducing oxidative stress and inflammation, restoring antioxidant balance, and improving lipid profiles. Histopathological analysis revealed severe myocardial disorganization, necrosis, and fibrosis in the stanozolol group, whereas the allicin-treated group exhibited preserved myocardial structure with reduced collagen deposition.</p><p><strong>Conclusion: </strong>Allicin significantly mitigates stanozolol-induced cardiotoxicity by reducing oxidative stress, inflammation, lipid dysregulation, and myocardial damage, as evidenced by biochemical and histopathological findings. These results suggest that allicin may serve as a potential therapeutic agent to counteract the cardiovascular risks associated with anabolic steroid use.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advancements and evaluation of multifactor-induced murine models for gastric cancer.","authors":"Yiqing Wang, Liang Zhang, Weixu Feng, Wanfeng Liu, Xiangyang Xue, Shiyu Feng","doi":"10.1002/ame2.70043","DOIUrl":"https://doi.org/10.1002/ame2.70043","url":null,"abstract":"<p><p>As one of the most prevalent gastrointestinal malignancies in humans, gastric cancer (GC) is often detected at an advanced stage, resulting in a poor prognosis and ranking it the fifth leading cause of cancer-related deaths. Due to their high genomic correlation with humans, mice are ideal in vivo models for investigating GC-related pathogenesis and therapeutic interventions. This review provides an overview of different GC models, including genetically engineered, transplantation-based models, and chemically or biologically induced models, and discusses the recent advancements for each type, highlighting their unique contributions to the field. In addition, it summarizes the strengths, limitations, and typical applications of these models and offers a critical assessment of their applicability in research while acknowledging their current limitations in fully mirroring human GC progression. Furthermore, we analyze how each model accurately recapitulates the complexities of human GC and evaluate their potential for clinical translation. This review provides a reference for model selection in future GC research.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term immunosuppression of rabbits through oral tacrolimus administration.","authors":"Alexane Thibodeau, Todd Galbraith, Oumayma Hayouni, Hélène T Khuong, François Berthod","doi":"10.1002/ame2.70038","DOIUrl":"https://doi.org/10.1002/ame2.70038","url":null,"abstract":"<p><strong>Background: </strong>The absence of well-established immunosuppressed rabbit models poses a significant hurdle in xenograft experiments. Tacrolimus has been identified as a highly promising immunosuppressive agent for rabbits. However, determining the optimal dosage and route of administration to minimize toxicity while maintaining efficacy remains challenging.</p><p><strong>Methods: </strong>In this study, we investigated the effect of orally administered tacrolimus in rabbits, with an aim to achieve a whole blood target trough level of 3-10 ng/mL, and looked at signs of tissue rejection after the transplantation of a human nerve conduit to repair a severed fibular nerve. An oral dosage range of 0.25-1.5 mg/kg/d was studied for up to 1 year in 63 New Zealand rabbits.</p><p><strong>Results: </strong>We demonstrated the feasibility of long-term grafting in rabbits while maintaining safe immunosuppression, with side effects mainly limited to diarrhea. Customizing the administered dose proved crucial for graft efficacy and low toxicity, which translated into 100% individual survival. We suggest an oral tacrolimus dose of 1.0-1.5 mg/kg depending on individual heterogeneity and recommend to implement a close therapeutic drug monitoring in the rabbits to maintain a whole blood tacrolimus trough level within the range of 5-12 ng/mL, as levels below 5 ng/mL showed signs of inflammation in the graft.</p><p><strong>Conclusion: </strong>The oral administration of tacrolimus enabled efficient immunosuppression of rabbits over a 1-year period without significant side effects or loss of animals.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}