Journal of analytical techniques and research最新文献

Plasmon-Induced Flexibility and Refractive Index Measurement in A Sensor Designed by a Cavity, Two Rings, Two Teeth and Two Plasmonic Waveguides 由一个腔、两个环、两个齿和两个等离子体波导设计的传感器的等离子体诱导柔韧性和折射率测量

Journal of analytical techniques and research Pub Date : 2023-01-01 DOI: 10.26502/jatr.36

Hamid Abbasi

引用次数: 0

Design of an Advanced Plasmonic Sensor (Consisting of A Quadrilateral Cavity, Three Rings with Different Dimensions and Two Waveguides) Using Refractive Index Change 利用折射率变化设计一种先进的等离子体传感器(由一个四边形腔、三个不同尺寸的环和两个波导组成)

Journal of analytical techniques and research Pub Date : 2023-01-01 DOI: 10.26502/jatr.37

Hamid Abbasi

引用次数: 0

Dynamic Regulation of MALAT1 in Leader Cell Formation and Collective Cancer Invasion MALAT1在领导细胞形成和集体肿瘤侵袭中的动态调控

Journal of analytical techniques and research Pub Date : 2023-01-01 DOI: 10.26502/jatr.35

Purnima Tyagi, Akhilesh Kumar Saini, Jitendra Kumar.

引用次数: 0

Conventional Frontal Air Sinus Imaging in Personal Identification among Adult Bangladeshi 常规额空气窦成像在孟加拉成人个人识别中的应用

Journal of analytical techniques and research Pub Date : 2023-01-01 DOI: 10.26502/jatr.34

Farzana Maqsood, ShamimAra ., Md. Abdur Rashid

引用次数: 0

Phenotypic variability in expressivity and deleterious capability of the homozygous UGT1A1*28 genotype 纯合子UGT1A1*28基因型表达能力和有害能力的表型变异

Journal of analytical techniques and research Pub Date : 2023-01-01 DOI: 10.26502/jatr.33

R. Rodríguez-López, J. Vivancos, Ana Cristina Requena Piedrabuena, Irene Ferrer Bolufer

引用次数: 0

One for All and All in One: Modified Silica Kit-based Protocol for simultaneous sample-specific Extraction of DNA from a Variety of Source Materials 一对一和一体化：基于改良二氧化硅试剂盒的方案，用于从各种源材料中同时提取DNA的样品特异性

Journal of analytical techniques and research Pub Date : 2022-01-10 DOI: 10.21203/rs.3.pex-1696/v1

W. M. Schmerer

引用次数: 0

Comparison of Bis-Plus-D and API 20 Strep for the Identification of Streptococci in the Laboratory of the University Hospital of Befelatanana Antananarivo Madagascar Bis-Plus-D与API 20 Strep在马达加斯加塔那那利佛贝费拉塔纳纳大学医院实验室鉴定链球菌的比较

Journal of analytical techniques and research Pub Date : 2022-01-01 DOI: 10.26502/jatr.30

Z. Rakotovao-Ravahatra, Jimmy Anders Antilahy, Joely Nirina Rakotovao-Ravahatra, Andriamiadana Luc Rakotovao

{"title":"Comparison of Bis-Plus-D and API 20 Strep for the Identification of Streptococci in the Laboratory of the University Hospital of Befelatanana Antananarivo Madagascar","authors":"Z. Rakotovao-Ravahatra, Jimmy Anders Antilahy, Joely Nirina Rakotovao-Ravahatra, Andriamiadana Luc Rakotovao","doi":"10.26502/jatr.30","DOIUrl":"https://doi.org/10.26502/jatr.30","url":null,"abstract":"Background: In medical analysis laboratories, techniques for identifying bacteria are currently becoming more and more numerous. The objective of this study is to compare the Bis-Plus-D and the API 20 Strep for the identification of streptococci Methods: During the study period, 22 Gram-positive coci isolates were identified using Api 20 Strep and Bis-Plus-D. Results: During the study period, 22 isolates of streptococci were identified simultaneously using Bis-Plus-D and Api 20 Strep. The streptococci identified were represented by Streptococcus anginosus, Streptococcus agalactiae, Streptococcus uberis, Enterococcus columbae, Streptococcus pyogenes, Streptococcus constellatus, Enterococcus durans Enterococcus casseliflavus, Enterococcus faecalis and Enterococcus faecium. This study showed 50% of discordant results between Bis-Plus-D and Api 20 Strep. Regarding the bacterial identification qualities, they were all excellent (100%) for Api 20 Strep. For Bis-Plus-D, the identification qualities were excellent for 36.4% of bacteria, very good for 36.4% of bacteria, good for 22.7% of bacteria and intermediate for 4.5% of bacteria. Concerning the probability scores for exact identification of bacteria, they vary between 82% to 100% for Api 20 Strep and between 62.6% to 99.9% for Bis-Plus-D. Conclusion: Api 20 Strep remains the best identification bacterial system and can be used by all bacteriology laboratories for good identification of bacterial species.","PeriodicalId":93773,"journal":{"name":"Journal of analytical techniques and research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69348042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Measurement of Low Avidity, Polyreactive Immunoglobulin G Antibodies with Increased Sensitivity by Using Low Ionic Strength Buffers 使用低离子强度缓冲液测量低亲和度、多反应性免疫球蛋白G抗体的灵敏度

Journal of analytical techniques and research Pub Date : 2022-01-01 DOI: 10.26502/jatr.24

A. Engelmaier, Harald Arno Butterweck, A. Weber

{"title":"Measurement of Low Avidity, Polyreactive Immunoglobulin G Antibodies with Increased Sensitivity by Using Low Ionic Strength Buffers","authors":"A. Engelmaier, Harald Arno Butterweck, A. Weber","doi":"10.26502/jatr.24","DOIUrl":"https://doi.org/10.26502/jatr.24","url":null,"abstract":"Measurement of Low Avidity, Polyreactive Immunoglobulin G Antibodies with Increased Sensitivity by Using Low Abstract Low avidity polyreactive immunoglobulin G (IgG) antibodies have a broad range of affinity with dissociation constants of 10 -5 to 10 -8 . Both their low concentration and avidity require low dilutions for measurement in solid-phase assays, which can cause issues due to the high total IgG levels probably interacting with the solid support. Here, we show that using an assay buffer system with low conductivity obviously increases the sensitivity of direct ELISAs. The 40 42 carbonate 9.5, were coated to HEPES, pH 7.4. Both buffers contained 10 mg/mL human serum albumin. Serial dilution series were loaded to antigen-coated and blank wells after blocking the plate with the respective dilution buffer. Detection of binding was achieved by an anti-human IgG peroxidase and peroxidase staining. Reduced dilution buffer’s conductivity increased signals by at least 50-fold without affecting the binding selectivity. Within the NaCl concentration range from 0 to 150 mM in 20 mM HEPES buffer, the conductivity correlated well with the signal height (R²=0.98). Competition experiments confirmed the assay’s adequate selectivity. In summary, buffer systems with low conductivity significantly increase the signals of direct ELISA without negatively affecting their selectivity. This simple assay modification increases the validity of results obtained by direct ELISAs for measurement of autoantibodies. and regulation of","PeriodicalId":93773,"journal":{"name":"Journal of analytical techniques and research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69348002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Cognitive Reserve using Near Infrared Spectroscopy. 近红外光谱评价认知储备。

Journal of analytical techniques and research Pub Date : 2022-01-01 Epub Date: 2022-05-19

Andrei V Medvedev

{"title":"Assessment of Cognitive Reserve using Near Infrared Spectroscopy.","authors":"Andrei V Medvedev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cognitive reserve (CR) is the ability to preserve cognitive functions in the presence of brain pathology. In the context of Alzheimer's disease (AD), patients with higher CR show better cognitive performance relative to brain damage therefore higher CR reduces the risk of dementia. There is a strong need to develop a neurophysiological biomarker of CR given the growing interest in understanding protective brain mechanisms in AD. FMRI studies indicate that frontoparietal network plays an important role in cognitive reserve. We calculated intraregional functional connectivity of lateral prefrontal cortex (FC LPFC) using functional near infrared spectroscopy (fNIRS) in the resting state of 13 healthy individuals who were also assessed for IQ and motoric skills (the Purdue Pegboard test, PPT). FC LPFC was found to positively correlate with IQ (a proxy measure of cognitive reserve) while showing a lack of or negative correlation with the PPT scores. The results demonstrate that the cost-effective, noninvasive and widely applicable fNIRS technology can be used to evaluate cognitive reserve in individuals at risk for and patients with AD with possible numerous applications in the context of healthy aging and other age-related cognitive disorders.</p>","PeriodicalId":93773,"journal":{"name":"Journal of analytical techniques and research","volume":"4 2","pages":"89-101"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9394433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40634681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hetero-Molecular Interactions in Polar-Nonpolar Binary Mixture of Different Compositions and Studies on their Thermodynamic Properties 不同组成极性-非极性二元混合物中的杂分子相互作用及其热力学性质研究

Journal of analytical techniques and research Pub Date : 2022-01-01 DOI: 10.26502/jatr.26

M. Rahman, M. Habibullah

引用次数: 0