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Influence of semolina characteristics and pasta-making process on the physicochemical, structural, and sensorial properties of commercial durum wheat spaghetti 粗粉特性和制面工艺对商用硬质小麦意大利面的理化、结构和感官特性的影响
Frontiers in food science and technology Pub Date : 2024-06-07 DOI: 10.3389/frfst.2024.1416654
S. Carpentieri, Dominique Larrea-Wachtendorff, Giovanna Ferrari
{"title":"Influence of semolina characteristics and pasta-making process on the physicochemical, structural, and sensorial properties of commercial durum wheat spaghetti","authors":"S. Carpentieri, Dominique Larrea-Wachtendorff, Giovanna Ferrari","doi":"10.3389/frfst.2024.1416654","DOIUrl":"https://doi.org/10.3389/frfst.2024.1416654","url":null,"abstract":"Introduction: This study aimed to investigate the effects of semolina characteristics and pasta-making processing on the quality of durum wheat spaghetti (A, B, and C) using a reverse engineering approach.Methods: Proximate composition, color, granulometry, pasting and thermal properties were determined to characterize semolina, while pasting, thermal and cooking properties, microstructure and sensory profile were evaluated for spaghetti.Results and Discussion: The sensory profile of sample A, in terms of higher yellowness and hardness, lower stickiness, agreed with the higher transition temperatures (60.07–74.6 °C) and enthalpy of amylose-lipid complexes, degree of starch gelatinization, pasting viscosities and compression force, lower water absorption, cooking losses (4.78 g/100 g), and adhesiveness (0.39 N/mm). Moreover, the microstructural analysis showed a network with denser areas, and starch granules strongly embedded in the gluten matrix for sample A with respect to samples B and C. These results might be ascribed to the semolina characteristics. Sample A was richer in starch (71.4%), lipid (1.6%), and protein (14.0%), and the higher drying temperatures affected protein crosslinking, starch organization, cooking properties, and appearance of spaghetti. The approach used in this study could be suitable in gathering the different phenomena occurring in pasta production process which concur to give spaghetti its distinctive quality.","PeriodicalId":93753,"journal":{"name":"Frontiers in food science and technology","volume":" 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141373025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ozone treatment of meat and meat products: a review 肉和肉制品的臭氧处理:综述
Frontiers in food science and technology Pub Date : 2024-06-06 DOI: 10.3389/frfst.2024.1351801
B. Giménez, Noemí Zaritzky, N. Graiver
{"title":"Ozone treatment of meat and meat products: a review","authors":"B. Giménez, Noemí Zaritzky, N. Graiver","doi":"10.3389/frfst.2024.1351801","DOIUrl":"https://doi.org/10.3389/frfst.2024.1351801","url":null,"abstract":"Ozone treatment is a non-thermal method for disinfection; ozone is a powerful oxidizing agent that has been shown to be effective in reducing microbial load, extending the shelf life of meat products. This mini-review covers the analysis of the antimicrobial ozone activity in different meats (beef, poultry, pork, seafood, etc.), emphasizing the ozone application methods (liquid or gaseous phase), the applied concentrations and contact times and the effects of ozone treatment on meat quality, safety, and sensory properties. It has been demonstrated that ozone is effective against a broad range of microorganisms, including both Gram-positive and Gram-negative bacteria, spores, and vegetative cells. The efficacy of ozone depends on various factors, such as concentration, type of treatment, temperature, and presence of organic material. Ozone treatment, known for its rapid decomposition and lack of residue, provides an environmentally friendly alternative to traditional chemical sanitizers. Ozone treatments exhibit promising results in enhancing the safety and extending the shelf life of meat products. According to the findings, the application of ozone is an effective technology for prolonging the shelf life of different types of meats and meat products, requiring careful establishment of conditions on a case-by-case basis.","PeriodicalId":93753,"journal":{"name":"Frontiers in food science and technology","volume":"17 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141378285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioactive composition and anti-hyperglycemic properties of biofortified yellow maize-based gluten-free cookies 生物强化黄玉米无筋饼干的生物活性成分和抗高血糖特性
Frontiers in food science and technology Pub Date : 2024-05-22 DOI: 10.3389/frfst.2024.1365021
K. O. Abdulrazaaq, Yunus Temitayo Imam, E. A. Irondi, E. Ajani
{"title":"Bioactive composition and anti-hyperglycemic properties of biofortified yellow maize-based gluten-free cookies","authors":"K. O. Abdulrazaaq, Yunus Temitayo Imam, E. A. Irondi, E. Ajani","doi":"10.3389/frfst.2024.1365021","DOIUrl":"https://doi.org/10.3389/frfst.2024.1365021","url":null,"abstract":"The use of gluten-free ingredients in the baking of cookies is increasing due to gluten-associated disorder and other nutritional-related non-communicable diseases, and consumer's pursuit for health-promoting foods. In this study, gluten-free cookies (GFC) were made from biofortified yellow maize supplemented with sodium carboxyl methylcellulose (SCMC), a reference hydrocolloid, and Brachystegia eurycoma flour (BEF) at four different proportions (2.5, 5, 7.5, and 10%), respectively. The GFC were then assessed for their bioactive components (flavonoids, tannins, and saponins), carotenoids, starch-hydrolyzing enzymes (alpha-amylase and alphaglucosidase), and glycemic index. Cookies made from commercial wheat flour (100% CWF) served as control. The total flavonoid contents of the 100% BYF cookies (144.48 ± 0.18 mg/g) and saponins (1.04 ± 0.00 mg/g) were significantly (p < 0.05) higher than the 100% CWF cookies (93.84 ± 0.36 mg/g; 1.02 ± 0.00 mg/g). The total tannin contents of the cookies ranged from 3.12 ± 0.00 mg/g (100% CWF) to 4.87 ± 0.00 mg/g (2.5%BEF-97.5% BYF). The carotenoid profiles indicated the presence of lutein (range from 0.62 to 1.63 μg/g), β-carotene (range from 3.81 to 6.06 μg/g), β-cryptoxanthin (range from 0.19 to 1.75 μg/g) and zeaxanthin (range from 0.83 to 2.48 μg/g) in the cookies. Further, the GF cookies significantly (p < 0.05) inhibited the alpha-amylase (IC50 value ranged from 1.42 ± 0.01 μg/mL (100% BYF) to 2.63 ± 0.01 μg/mL (100% CWF)) and alpha-glucosidase with IC50 value range from 1.12 ± 0.01 μg/mL (2.5%SCMC-97.5% BYF) to 6.72 ± 0.01 (7.5%BEF-92.5% BYF). The GF cookies showed a low glycemic index (GI) value (GI < 50). The biofortified yellow maize-based GFC have significant amounts of bioactive compounds and anti-hyperglycemic properties, and low GI. Hence, they can serve as functional baked food with potential health benefits.","PeriodicalId":93753,"journal":{"name":"Frontiers in food science and technology","volume":"86 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141112104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Binderless particleboards obtained 100% from winery by-products for the packaging industry 用于包装工业的无粘合剂刨花板 100% 由酿酒厂副产品制成
Frontiers in food science and technology Pub Date : 2024-04-18 DOI: 10.3389/frfst.2024.1376415
Raquel A. Fernandes, Sandro Lopes, N. Ferreira, Jorge Santos, Jorge M. Martins, Luisa H. Carvalho
{"title":"Binderless particleboards obtained 100% from winery by-products for the packaging industry","authors":"Raquel A. Fernandes, Sandro Lopes, N. Ferreira, Jorge Santos, Jorge M. Martins, Luisa H. Carvalho","doi":"10.3389/frfst.2024.1376415","DOIUrl":"https://doi.org/10.3389/frfst.2024.1376415","url":null,"abstract":"The valorization of bioeconomy by-products holds immense significance in achieving sustainability goals and fostering resource efficiency, not only to reduce the amount of waste generated but also to significantly decrease the carbon footprint associated with several industrial fields, by promoting circular economy. The wine industry is not only one of the major contributors for world’s economy but also a great producer of by-products, with no valorization process associated. Grape stalks are a great source of chemical molecules, as polyphenols, that can be applied in the production of bio-adhesives. In the present work, grape stalk particles were used as raw material to obtain a highly rich extract with bonding properties through solid/liquid extraction. Different extraction agents were tested (water, NaOH, and Na2CO3) at varied concentrations (0.1%, 0.5%, and 1.0%, respectively). Additionally, extracts were chemically characterized, and the bonding capacity was also evaluated. Using NaOH 1.0%, an extraction efficiency of 8.9% and a total content of polyphenols of 1.4% were achieved. Moreover, all extracts presented an interesting bonding capacity (>1.0 MPa) by hot-press at 120°C for 120 s. The composite panel produced using grape stalk adhesive and vine strain particles fulfills the requirements for type P1 particleboards in terms of internal bond strength, reaching 0.66 MPa. The mechanical and wettability properties of composite material allow foreseeing a promising application in the food packaging industry.","PeriodicalId":93753,"journal":{"name":"Frontiers in food science and technology","volume":" 33","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140688141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hygienic practices and factors of complementary food preparation among mothers of children aged 6–24 months in Ethiopia: a systematic review and meta-analysis 埃塞俄比亚 6-24 个月儿童母亲的卫生习惯和辅食制作因素:系统回顾和荟萃分析
Frontiers in food science and technology Pub Date : 2024-04-11 DOI: 10.3389/frfst.2024.1240979
Agerie Mengistie Zeleke, Worku Chekol Tassew, Yeshiwas Ayale Ferede, Tadele Maru Andargie
{"title":"Hygienic practices and factors of complementary food preparation among mothers of children aged 6–24 months in Ethiopia: a systematic review and meta-analysis","authors":"Agerie Mengistie Zeleke, Worku Chekol Tassew, Yeshiwas Ayale Ferede, Tadele Maru Andargie","doi":"10.3389/frfst.2024.1240979","DOIUrl":"https://doi.org/10.3389/frfst.2024.1240979","url":null,"abstract":"Background: A child’s transition to complementary food (CF) feeding is important, and it should be ensured that CF is prepared, stored, and fed in a hygienic manner using clean hands, dishes, and utensils to prevent infections. However, there are no comprehensive review statistics available at the national level. Hence, the aim of this study is to determine the hygienic practices of complementary food preparation and its factors among Ethiopian women with children between the ages of 6 and 24 months.Methods: On the nationwide prevalence of hygienic practices during the preparation of complementary foods and their determinants among mothers of children aged 6–24 months in Ethiopia, a systematic review and meta-analysis were performed. We searched in PubMed, ScienceDirect, Scopus, African Journal Online (AJOL), Google Scholar, and the websites to identify studies that were published until March 2023. We performed this review following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. A random-effects meta-analysis was done using StatsDirect statistical software and Open Meta Analyst to estimate the prevalence of hygienic practices, and the results were reported in a forest plot. Simple charts and tables were used to summarize the evidence on complementary food preparation and determinates.Results: Nine studies involving 4,403 mothers were included in this Ethiopian study. The overall prevalence of good hygienic practices during complementary food preparation among mothers who have children aged 6–24 months was 46.53% (95% CI: 28.13–64.94) (p < 0.0001). Having a separate kitchen (AOR = 6.78; 95% CI: 1.04–44.20), having a three-bowl washing facility in their houses (AOR = 10.61; 95% CI: 7.31–15.41), mothers’ access to media (AOR = 4.81; 95% CI: 1.23–18.82), and urban residence (AOR = 4.78; 95% CI: 2.73–8.38) were the predictors of good hygienic practice during complementary food preparation.Conclusion: Regarding the prevalence of hygienic procedures among the mothers of children aged 6–24 months nationwide when preparing complementary foods, there is a significant lack of hygienic behavior among Ethiopian mothers. Hence, the Ethiopian ministries of health focus on designing complementary food guidelines for the prenatal healthcare system. Furthermore, health extension workers are also better advised to maintain food hygiene during prenatal care visits, which is recommended to improve good hygienic practices during complementary food preparation in Ethiopia.","PeriodicalId":93753,"journal":{"name":"Frontiers in food science and technology","volume":"44 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140716191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unreliability of clustering results in sensory studies and a strategy to address the issue 感官研究中聚类结果的不稳定性及解决策略
Frontiers in food science and technology Pub Date : 2024-04-10 DOI: 10.3389/frfst.2024.1271193
Rajesh Kumar, Edgar Chambers
{"title":"Unreliability of clustering results in sensory studies and a strategy to address the issue","authors":"Rajesh Kumar, Edgar Chambers","doi":"10.3389/frfst.2024.1271193","DOIUrl":"https://doi.org/10.3389/frfst.2024.1271193","url":null,"abstract":"Researchers commonly use hierarchical clustering (HC) or k-means (KM) for grouping products, attributes, or consumers. However, the results produced by these approaches can differ widely depending on the specific methods used or the initial “seed” aka “starting cluster centroid” chosen in clustering. Although recommendations for various clustering techniques have been made, the realities are that objects in groups can, and do, change their clusters. That can impact interpretation of the data. Researchers usually does not run the clustering algorithms multiple times to determine stability, nor do they often run multiple methods of clustering although that has been recommended previously. This study applied hierarchical agglomerative clustering (HAC), KM and fuzzy clustering (FC) to a large descriptive sensory data set and compared attribute clusters from the methods, including multiple iterations of same methods. Sensory attributes (objects) shuffled among clusters in varying ways, which could provide different interpretations of the data. That frequency was captured in the KM output and used to form the “best possible” clusters via manual clustering (MC). The HAC and FC results were studied and compared with KM results. Attribute correlation coefficients also were compared with clustering information. Using results from one clustering approach may not be reliable, and results should be confirmed using other clustering approaches. A strategy that combines multiple clustering approaches, including a MC process is suggested to determine consistent clusters in sensory data sets.","PeriodicalId":93753,"journal":{"name":"Frontiers in food science and technology","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140717765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microbial ecology and fermentation of Coffea canephora 可可豆的微生物生态和发酵
Frontiers in food science and technology Pub Date : 2024-04-05 DOI: 10.3389/frfst.2024.1377226
Patrícia Campos Bernardes, J. Coelho, P. Martins, R. Schwan
{"title":"Microbial ecology and fermentation of Coffea canephora","authors":"Patrícia Campos Bernardes, J. Coelho, P. Martins, R. Schwan","doi":"10.3389/frfst.2024.1377226","DOIUrl":"https://doi.org/10.3389/frfst.2024.1377226","url":null,"abstract":"Recent investigations into the microbiota and fermentation of Coffea canephora fruits and beans have yielded limited advancements globally, highlighting a developing field of study. Consequently, this review seeks to consolidate existing literature through an analysis focusing on the microbiota and fermentation processes inherent in the postharvest processing of Canephora coffee. To this end, a comprehensive examination of the principal microorganisms inherent to this species, the application of starter cultures in fermentation, and the repercussions of fermentation on the chemical and sensory attributes of the beverage will be expounded. These investigations underscore the influence exerted by the fermentation process and the introduction of microorganism inoculation on Canephora coffees’ chemical composition and sensory characteristics. Fermentation emerges as a mechanism facilitating the modification of coffee flavor and aroma, thereby presenting avenues for innovative enhancements in producing distinct Canephora coffee beverages. Research to assess the microbiota of Canephora coffees from various origins has the potential to advance our understanding of the microbial ecology specific to this species. Such studies will play a crucial role in identifying pertinent starter cultures that could be used to produce high-quality coffees.","PeriodicalId":93753,"journal":{"name":"Frontiers in food science and technology","volume":"16 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140738053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of processing conditions of starch-based hydrogels produced by high-pressure processing (HPP) using response surface methodology 利用响应面方法优化通过高压加工(HPP)生产的淀粉基水凝胶的加工条件
Frontiers in food science and technology Pub Date : 2024-03-13 DOI: 10.3389/frfst.2024.1376044
Katerina Koshenaj, Giovanna Ferrari
{"title":"Optimization of processing conditions of starch-based hydrogels produced by high-pressure processing (HPP) using response surface methodology","authors":"Katerina Koshenaj, Giovanna Ferrari","doi":"10.3389/frfst.2024.1376044","DOIUrl":"https://doi.org/10.3389/frfst.2024.1376044","url":null,"abstract":"Introduction: This study aimed to determine the optimal processing conditions to produce stable starch-based hydrogels by high-pressure processing (HPP) via response surface methodology.Methods: The experiments were carried out with different starch suspensions, namely rice, corn, wheat, and tapioca starch, at a concentration in the range of 10%–40% w/w, processed at a pressure level of 600 MPa and holding times between 5 min and 15 min. Gel formation was assessed by determining the gelatinization extent and structuring level of the samples.Results and discussion: The results demonstrated that starch/water ratio and holding time had a significant impact on gel formation in HPP treatments. Various degrees of gelatinization were observed in the treated samples due to the water absorption capacity of the starch and the molecular interactions between water and starch occurring during gelatinization. Moreover, a highly structured hydrogel formed at starch concentrations higher than 25% (w/w), whereas when starch concentration was less than 20% (w/w) lower-structured hydrogels formed, as confirmed by the values of the efficiency index measured. Completely gelatinized, highly structured, and stable HPP hydrogels were obtained from starch solutions treated at the optimized processing conditions.","PeriodicalId":93753,"journal":{"name":"Frontiers in food science and technology","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140246125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seed storage allergens tackled via next-generation research assistant 通过新一代研究助手解决种子储藏过敏原问题
Frontiers in food science and technology Pub Date : 2024-03-12 DOI: 10.3389/frfst.2024.1372770
Adriana Rita Evangelista, C. G. Amoroso, Chiara Nitride, Giuseppe Andolfo
{"title":"Seed storage allergens tackled via next-generation research assistant","authors":"Adriana Rita Evangelista, C. G. Amoroso, Chiara Nitride, Giuseppe Andolfo","doi":"10.3389/frfst.2024.1372770","DOIUrl":"https://doi.org/10.3389/frfst.2024.1372770","url":null,"abstract":"The expanding consumption of plant proteins in the diet to overcome the environmental issues associated with animal proteins is increasing the incidence of food-induced allergic reactions. One of the 21st-century research drivers in agriculture sciences is the development and validation of concrete approaches for modulating the expression of allergenic proteins in crops before harvesting. The increasing incidence of plant food allergies is primarily induced by seed storage proteins that clinicians are experiencing recently because of the more predominant use of plant-derived proteins in the food industry. Increased availability of high-throughput technologies has generated an ever-growing number of omics data, allowing us to have better structural knowledge of SSPs and molecular properties that can inform the allergenicity assessment. The recent systems for targeted genome engineering, without double-strand DNA breaks, allow the introduction of precise modifications directly into commercial plant species. Artificial intelligence is significantly transforming scientific research across every stage, assisting scientists, processing large-scale data, making predictions, automating tasks. During this epochal change, marked by the encounter between artificial intelligence and synthetic biology, a next-generation research assistant (NGA) is coming alive. Here, we propose a new conceptual vision to facilitate and speed up the editing of cross-reactivity sites to obtain hypoallergenic cultivars and avoid pleiotropic effects. Finally, we discuss the potential applications of this new way to conceive the research. NGA may be undoubtedly capable of managing the evolution of SPP allergies through the prediction of novel epitopes, as well as the prediction of immunological response mechanisms.","PeriodicalId":93753,"journal":{"name":"Frontiers in food science and technology","volume":"121 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140250473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mechanisms of microbial photoinactivation by curcumin’s micellar delivery 姜黄素胶束递送的微生物光活化机制
Frontiers in food science and technology Pub Date : 2024-03-08 DOI: 10.3389/frfst.2024.1361817
Victor Ryu, M. Ghoshal, Piyanan Chuesiang, Silvette Ruiz-Ramírez, Lynne McLandsborough, M. Corradini
{"title":"Mechanisms of microbial photoinactivation by curcumin’s micellar delivery","authors":"Victor Ryu, M. Ghoshal, Piyanan Chuesiang, Silvette Ruiz-Ramírez, Lynne McLandsborough, M. Corradini","doi":"10.3389/frfst.2024.1361817","DOIUrl":"https://doi.org/10.3389/frfst.2024.1361817","url":null,"abstract":"Introduction: Microbial photoinactivation using UV light can be enhanced by the addition of food-grade photosensitizers (PSs), such as curcumin. Micellization of curcumin can improve its stability and antimicrobial activity. The objective of this study was to investigate the potential mechanisms that contribute to the photoinactivation of Escherichia coli O157: H7 and Listeria innocua by curcumin-loaded surfactant solutions produced with Surfynol 465 (S465) or Tween 80 (T80) below, near, and above their critical micelle concentration (CMC).Methods: Stock curcumin-surfactant solutions were produced with S465 or T80 (5 mM sodium citrate buffer, pH 3.5). Mixtures of each bacterial suspension (initial inoculum = 6 LogCFU/mL), 1 µM curcumin, and surfactants were irradiated with UV-A light (λ = 365 nm) for 5 min. Microbial recovery after treatments was assessed by monitoring the growth of the treated E. coli O157: H7 or L. innocua using an oCelloscope™. The growth curves were characterized using a modified logistic model.Results and Discussion: Both gram-positive and gram-negative bacteria showed less and slower recovery when treated with curcumin-S465 (near or at CMC) than curcumin-T80 solutions after irradiation. FLIM micrographs suggested that curcumin was preferentially localized at the cell membrane when S465 was present, as evidenced by its longer lifetimes in samples treated with curcumin-S465 solutions. Washing after treatment resulted in the removal of loosely bound or unbound S465-curcumin micelles; hence, both E. coli O157: H7 and L. innocua recovery was faster. This suggested that curcumin partitioning has a significant role in microbial photoinactivation, possibly due to the production of reactive oxygen species (ROS) closer to/within the membrane. The permeability of the membrane of E. coli O157: H7, as inferred from the Live/Dead cell assay, increased when S465 was present, suggesting that S465 can also facilitate inactivation by disrupting the membrane and by favoring the localization of curcumin adjacent to the cell membrane. Therefore, a synergistic antimicrobial effect is observed when curcumin is present alongside S465 at concentrations below or near its CMC due to the disruption of the cell membrane by S465.","PeriodicalId":93753,"journal":{"name":"Frontiers in food science and technology","volume":"44 26","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140076955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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